Spatiotemporal Control over Cell Proliferation and Differentiation for Tissue Engineering and Regenerative Medicine Applications Using Silk Fibroin Scaffolds

2020 ◽  
Vol 3 (6) ◽  
pp. 3476-3493 ◽  
Author(s):  
Smita Patil ◽  
Vartika Dhyani ◽  
Tejinder Kaur ◽  
Neetu Singh
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Regenerative Medicine ◽  
Silk Fibroin ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Spatiotemporal Control

Neural stem cell proliferation and differentiation in the conductive PEDOT-HA/Cs/Gel scaffold for neural tissue engineering

2017 ◽  
Vol 5 (10) ◽  
pp. 2024-2034 ◽  
Author(s):  
Shuping Wang ◽  
Shui Guan ◽  
Jianqiang Xu ◽  
Wenfang Li ◽  
Dan Ge ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Regenerative Medicine ◽  
Neural Tissue ◽  
Neural Tissue Engineering ◽  
Stem Cell Proliferation ◽  
Neural Stem Cell Proliferation ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

Engineering scaffolds with excellent electro-activity is increasingly important in tissue engineering and regenerative medicine.

scholarly journals Electrical stimulation as a novel tool for regulating cell behavior in tissue engineering

2019 ◽  
Vol 23 (1) ◽  
Author(s):  
Cen Chen ◽  
Xue Bai ◽  
Yahui Ding ◽  
In-Seop Lee
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Electrical Stimulation ◽  
Intracellular Signaling ◽  
Cell Alignment ◽  
Mechanism Study ◽  
Regeneration Medicine ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Underlying Mechanisms

AbstractRecently, electrical stimulation as a physical stimulus draws lots of attention. It shows great potential in disease treatment, wound healing, and mechanism study because of significant experimental performance. Electrical stimulation can activate many intracellular signaling pathways, and influence intracellular microenvironment, as a result, affect cell migration, cell proliferation, and cell differentiation. Electrical stimulation is using in tissue engineering as a novel type of tool in regeneration medicine. Besides, with the advantages of biocompatible conductive materials coming into view, the combination of electrical stimulation with suitable tissue engineered scaffolds can well combine the benefits of both and is ideal for the field of regenerative medicine. In this review, we summarize the various materials and latest technologies to deliver electrical stimulation. The influences of electrical stimulation on cell alignment, migration and its underlying mechanisms are discussed. Then the effect of electrical stimulation on cell proliferation and differentiation are also discussed.

scholarly journals Regulation of Redox Homeostasis by Nonthermal Biocompatible Plasma Discharge in Stem Cell Differentiation

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Ying Li ◽  
Eun Ha Choi ◽  
Ihn Han
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Redox Homeostasis ◽  
Stem Cell Differentiation ◽  
Redox Biology ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

Recently, a growing body of evidence has shown the role of reactive species as secondary messengers in cell proliferation and differentiation, as opposed to the harmful metabolism byproducts that they were previously solely recognized as. Thus, the balance of intracellular reduction-oxidation (redox) homeostasis plays a vital role in the regulation of stem cell self-renewal and differentiation. Nonthermal biocompatible plasma (NBP) has emerged as a novel tool in biomedical applications. Recently, NBP has also emerged as a powerful tool in the tissue engineering field for the surface modification of biomaterial and the promotion of stem cell differentiation by the regulation of intracellular redox biology. NBP can generate various kinds of reactive oxygen species (ROS) and reactive nitrogen species (RNS), which may play the role of the second passenger in the cell signaling network and active antioxidant system in cells. Herein, we review the current knowledge on mechanisms by which NBP regulates cell proliferation and differentiation through redox modification. Considering the importance of redox homeostasis in the regulation of stem cell differentiation, understanding the underlying molecular mechanisms involved will provide important new insights into NBP-induced stem cell differentiation for tissue engineering.

Polypyrrole-coated poly(l-lactic acid-co-ε-caprolactone)/silk fibroin nanofibrous membranes promoting neural cell proliferation and differentiation with electrical stimulation

2016 ◽  
Vol 4 (41) ◽  
pp. 6670-6679 ◽  
Author(s):  
Binbin Sun ◽  
Tong Wu ◽  
Juan Wang ◽  
Dawei Li ◽  
Jing Wang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Electrical Stimulation ◽  
Lactic Acid ◽  
Silk Fibroin ◽  
Oxidative Polymerization ◽  
Neural Cell ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Nanofiber Membranes

Conductive nanofiber membranes were developed by coating Ppy on PLCL/SF nanofibers via in situ oxidative polymerization.

Nanomaterial integration into the scaffolding materials for nerve tissue engineering: a review

2020 ◽  
Vol 31 (8) ◽  
pp. 843-872
Author(s):  
Hamidreza Arzaghi ◽  
Bashir Adel ◽  
Hossein Jafari ◽  
Shaghayegh Askarian-Amiri ◽  
Amin Shiralizadeh Dezfuli ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cell Proliferation ◽  
Nervous System ◽  
Stem Cell ◽  
Complex Network ◽  
Neural Regeneration ◽  
Stem Cell Proliferation ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

AbstractThe nervous system, which consists of a complex network of millions of neurons, is one of the most highly intricate systems in the body. This complex network is responsible for the physiological and cognitive functions of the human body. Following injuries or degenerative diseases, damage to the nervous system is overwhelming because of its complexity and its limited regeneration capacity. However, neural tissue engineering currently has some capacities for repairing nerve deficits and promoting neural regeneration, with more developments in the future. Nevertheless, controlling the guidance of stem cell proliferation and differentiation is a challenging step towards this goal. Nanomaterials have the potential for the guidance of the stem cells towards the neural lineage which can overcome the pitfalls of the classical methods since they provide a unique microenvironment that facilitates cell–matrix and cell–cell interaction, and they can manipulate the cell signaling mechanisms to control stem cells’ fate. In this article, the suitable cell sources and microenvironment cues for neuronal tissue engineering were examined. Afterward, the nanomaterials that impact stem cell proliferation and differentiation towards neuronal lineage were reviewed.

Icariin Stimulates hFOB 1.19 Osteoblast Proliferation and Differentiation via OPG/RANKL Mediated by the Estrogen Receptor

2020 ◽  
Vol 22 (1) ◽  
pp. 168-175 ◽  
Author(s):  
Lin-Jun Sun ◽  
Chong Li ◽  
Xiang-hao Wen ◽  
Lu Guo ◽  
Zi-Fen Guo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Estrogen Receptor ◽  
Protein Expression ◽  
Chinese Herb ◽  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Expression Ratio ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Mrna And Protein Expression

Background:: Icariin (ICA), one of the main effective components isolated from the traditional Chinese herb Epimedium brevicornu Maxim., has been reported to possess extensive pharmacological actions, including enhanced sexual function, immune regulation, anti-inflammation, and antiosteoporosis. Methods:: Our study was designed to investigate the effect of ICA on cell proliferation and differentiation and the molecular mechanism of OPG/RANKL mediated by the Estrogen Receptor (ER) in hFOB1.19 human osteoblast cells. Results:: The experimental results show that ICA can stimulate cell proliferation and increase the activity of Alkaline Phosphatase (ALP), Osteocalcin (BGP) and I Collagen (Col I) and a number of calcified nodules. Furthermore, the mRNA and protein expression of OPG and RANKL and the OPG/ RANKL mRNA and protein expression ratios were upregulated by ICA. The above-mentioned results indicated that the optimal concentration of ICA for stimulating osteogenesis was 50ng/mL. Subsequent mechanistic studies comparing 50ng/mL ICA with an estrogen receptor antagonist demonstrated that the effect of the upregulated expression is connected with the estrogen receptor. In conclusion, ICA can regulate bone formation by promoting cell proliferation and differentiation and upregulating the OPG/RANKL expression ratio by the ER in hFOB1.19 human osteoblast cells.

scholarly journals Costimulation by B7 Modulates Specificity of Cytotoxic T Lymphocytes: A Missing Link That Explains Some Bystander T Cell Activation

1997 ◽  
Vol 186 (10) ◽  
pp. 1787-1791 ◽  
Author(s):  
Pan Zheng ◽  
Yang Liu
Keyword(s):  
Cell Proliferation ◽  
T Cells ◽  
Influenza Virus ◽  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
T Cell Proliferation ◽  
Target Cells ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

It has been proposed that some bystander T cell activation may in fact be due to T cell antigen receptor (TCR) cross-reactivity that is too low to be detected by the effector cytotoxic T lymphocyte (CTL). However, this hypothesis is not supported by direct evidence since no TCR ligand is known to induce T cell proliferation and differentiation without being recognized by the effector CTL. Here we report that transgenic T cells expressing a T cell receptor to influenza virus A/NT/68 nucleoprotein (NP) 366-374:Db complexes clonally expand and become effector CTLs in response to homologous peptides from either A/PR8/34 (H1N1), A/AA/60 (H2N2), or A/NT/68 (H3N2). However, the effector T cells induced by each of the three peptides kill target cells pulsed with NP peptides from the H3N2 and H2N2 viruses, but not from the H1N1 virus. Thus, NP366–374 from influenza virus H1N1 is the first TCR ligand that can induce T cell proliferation and differentiation without being recognized by CTLs. Since induction of T cell proliferation was mediated by antigen-presenting cells that express costimulatory molecules such as B7, we investigated if cytolysis of H1N1 NP peptide–pulsed targets can be restored by expressing B7-1 on the target cells. Our results revealed that this is the case. These data demonstrated that costimulatory molecule B7 modulates antigen specificity of CTLs, and provides a missing link that explains some of the bystander T cell activation.

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