scholarly journals Tumor-Associated Macrophages and Mast Cells Positive to Tryptase Are Correlated with Angiogenesis in Surgically-Treated Gastric Cancer Patients

2018 ◽  
Vol 19 (4) ◽  
pp. 1176 ◽  
Author(s):  
Giuseppe Sammarco ◽  
Cosmo Damiano Gadaleta ◽  
Valeria Zuccalà ◽  
Emre Albayrak ◽  
Rosa Patruno ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Mast Cells ◽  
Cancer Patients ◽  
Tumor Associated Macrophages ◽  
Gastric Cancer Patients

scholarly journals Infiltrating Mast Cells Correlate with Angiogenesis in Bone Metastases from Gastric Cancer Patients

2015 ◽  
Vol 16 (2) ◽  
pp. 3237-3250 ◽  
Author(s):  
Michele Ammendola ◽  
Ilaria Marech ◽  
Giuseppe Sammarco ◽  
Valeria Zuccalà ◽  
Maria Luposella ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Mast Cells ◽  
Bone Metastases ◽  
Cancer Patients ◽  
Gastric Cancer Patients

scholarly journals Extracellular vesicles shed from gastric cancer mediate protumor macrophage differentiation

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Atene Ito ◽  
Shunsuke Kagawa ◽  
Shuichi Sakamoto ◽  
Kazuya Kuwada ◽  
Hiroki Kajioka ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Ascitic Fluid ◽  
Extracellular Vesicles ◽  
Peritoneal Cavity ◽  
Peritoneal Dissemination ◽  
Tumor Associated Macrophages ◽  
Gastric Cancer Cells ◽  
Gastric Cancer Patients

Abstract Background Peritoneal dissemination often develops in gastric cancer. Tumor-associated macrophages (TAMs) are present in the peritoneal cavity of gastric cancer patients with peritoneal dissemination, facilitating tumor progression. However, the mechanism by which macrophages differentiate into tumor-associated macrophages in the peritoneal cavity is not well understood. In this study, the interplay between gastric cancer-derived extracellular vesicles (EVs) and macrophages was investigated. Methods The association between macrophages and EVs in peritoneal ascitic fluid of gastric cancer patients, or from gastric cancer cell lines was examined, and their roles in differentiation of macrophages and potentiation of the malignancy of gastric cancer were further explored. Results Immunofluorescent assays of the ascitic fluid showed that M2 macrophages were predominant along with the cancer cells in the peritoneal cavity. EVs purified from gastric cancer cells, as well as malignant ascitic fluid, differentiated peripheral blood mononuclear cell-derived macrophages into the M2-like phenotype, which was demonstrated by their morphology and expression of CD163/206. The macrophages differentiated by gastric cancer-derived EVs promoted the migration ability of gastric cancer cells, and the EVs carried STAT3 protein. Conclusion EVs derived from gastric cancer play a role by affecting macrophage phenotypes, suggesting that this may be a part of the underlying mechanism that forms the intraperitoneal cancer microenvironment.

scholarly journals Intratumor IL-17-Positive Mast Cells Are the Major Source of the IL-17 That Is Predictive of Survival in Gastric Cancer Patients

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e106834 ◽  
Author(s):  
Xiaosun Liu ◽  
Hailong Jin ◽  
Geer Zhang ◽  
Xianke Lin ◽  
Chao Chen ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Mast Cells ◽  
Cancer Patients ◽  
Gastric Cancer Patients

scholarly journals Mast Cells Positive to Tryptase and c-Kit Receptor Expressing Cells Correlates with Angiogenesis in Gastric Cancer Patients Surgically Treated

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Michele Ammendola ◽  
Rosario Sacco ◽  
Giuseppe Sammarco ◽  
Giuseppe Donato ◽  
Valeria Zuccalà ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Mast Cells ◽  
Cancer Patients ◽  
Tumour Tissue ◽  
7Th Edition ◽  
Complex Process ◽  
Kit Receptor ◽  
The Mean ◽  
Gastric Cancer Patients ◽  
Tryptase Inhibitors

Background. Angiogenesis is a complex process involved in both growth and progression of several human and animal tumours. Tryptase is a serin protease stored in mast cells granules, which plays a role in tumour angiogenesis. Mast cells (MCs) can release tryptase following c-Kit receptor (c-KitR) activation.Method. In a series of 25 gastric cancer patients with stage T3N2-3M0(by AJCC for Gastric Cancer 7th Edition), immunohistochemistry and image analysis methods were employed to evaluate in the tumour tissue the correlation between the number of mast cells positive to tryptase (MCPT), c-KitR expressing cells (c-KitR-EC), and microvascular density (MVD).Results. Data demonstrated a positive correlation between MCPT, c-KitR-EC, and MVD to each other. In tumour tissue the mean number of MCPT was 15, the mean number of c-KitR-EC was 20, and the mean number of MVD was 20. The Pearson test correlating MCPT and MVD, c-KitR-EC and MVD was significantly (r=0.64,P=0.001;r=0.66,P=0.041, resp.).Conclusion. In this pilot study, we suggest that MCPT and c-KitR-EC play a role in gastric cancer angiogenesis, so we think that several c-KitR or tryptase inhibitors such as gabexate mesilate and nafamostat mesilate might be evaluated in clinical trials as a new antiangiogenetic approach.

Infiltration Patterns of Immune Cells in Gastric Cancer and Their Clinical Correlation with Prognosis

2020 ◽  
Author(s):  
XiaoLi Wu ◽  
Hongbo Ma ◽  
YanYan Li
Keyword(s):  
Gastric Cancer ◽  
T Cells ◽  
Mast Cells ◽  
Nk Cells ◽  
Cancer Patients ◽  
Cd8 T Cells ◽  
Immune Cells ◽  
Immune Cell ◽  
M1 Macrophages ◽  
Gastric Cancer Patients

Abstract Abstract: Objective Gastric cancer is a malignant tumour that severely affects the health of patients. This study analyses the correlation between gastric cancer-infiltrating immune cell patterns and clinical prognosis and provides a scientific basis for the development of comprehensive tumour prevention and treatment strategies. Method Transcripts and related clinical data from 9-2019 for gastric cancer were downloaded from the TCGA database. The proportions of 22 kinds of immune cells were calculated by CIBERSORT software, and the correlation of each immune cell component ratio with tumour grade, clinical stage and overall survival (OS) was evaluated. Results A total of 413 gene transcript data sets were obtained from the TCGA database, including 381 for gastric cancer and 32 for normal tissues. The expression of various macrophages in tumour tissues was abundant. The immune cell composition, which included resting dendritic cells (p=0.02), M1 macrophages (p=0.031), resting mast cells (p=0.02), CD8 T cells (p=2.445e-04), M0 macrophages (p=6.353e-04), activated mast cells (p=0.006), neutrophils (p=0.003), resting NK cells (p=0.014), and gamma delta T cells (p=0.033), is related to the pathological grade. As the tumour stage of gastric cancer patients progresses, the proportion of some immune cells, including eosinophils (p=0.013), activated mast cells (p=0.042), neutrophils (p=0.007), and resting NK cells (p=0.036) gradually increases, while the proportion of other immune cells, for example, CD8 T cells (p=0.018), Tregs (p=0.039), M1 macrophages (p=0.018), and activated NK cells (p=0.042) gradually decreases. Higher expression of CD8 T cells suggests a better prognosis. Conclusion The composition of tumour-infiltrating immune cells differed greatly in different pathological grades and stages of gastric cancer. CD8 T cells can be used as a prognostic factor for gastric cancer patients.

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