scholarly journals Nrf2 Inhibits Periodontal Ligament Stem Cell Apoptosis under Excessive Oxidative Stress

2017 ◽  
Vol 18 (5) ◽  
pp. 1076 ◽  
Author(s):  
Yanli Liu ◽  
Hongxu Yang ◽  
Yi Wen ◽  
Bingyi Li ◽  
Yinhua Zhao ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cell ◽  
Cell Apoptosis ◽  
Periodontal Ligament ◽  
Periodontal Ligament Stem Cell

scholarly journals Effect of YAP on an Immortalized Periodontal Ligament Stem Cell Line

2019 ◽  
Vol 2019 ◽  
pp. 1-15
Author(s):  
Xiyan Chen ◽  
Qi Wang ◽  
Ke Gu ◽  
Aonan Li ◽  
Xucheng Fu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Line ◽  
Signaling Pathway ◽  
Cell Apoptosis ◽  
Periodontal Ligament ◽  
Hippo Signaling ◽  
Stem Cell Line ◽  
Tert Gene ◽  
Periodontal Ligament Stem Cell ◽  
Proliferation Ability

Objective. To establish an immortalized human periodontal ligament stem cell line (hPDLSC) and investigate whether and how YAP mediates the establishment of the stem cell line. Methods. Primary hPDLSCs were cultured and transfected with lentivirus containing the telomerase reverse transcriptase (TERT) gene. The expression of TERT was detected via the polymerase chain reaction (PCR) and real-time quantitative PCR (RT-PCR). Flow cytometry was employed to detect surface markers of hPDLSCs and TERT-hPDLSCs. The cell counting kit-8 (CCK-8) and 5-ethynyl-2’-deoxyuridine (EdU) methods were used to examine the proliferation ability of the cells. Flow cytometry and TUNEL staining were employed to examine the cell apoptosis rate. The β-galactosidase staining assay was used to assess the rate of cell senescence. The osteogenic differentiation ability of the cells was detected via alkaline phosphatase (ALP) staining and Alizarin red staining assays. BALB/c mice were employed to determine the tumorigenicity of TERT-hPDLSCs. The expression levels of YAP and other proteins in the Hippo signaling pathway were detected by Western blotting. Verteporfin was used to inhibit the binding of YAP to the downstream target gene TEAD. Results. TERT-hPDLSCs showed stable high expression of TERT, even at the thirtieth passage after transfection with lentivirus containing the TERT gene. Compared with primary hPDLSCs, TERT-hPDLSCs exhibited a stronger proliferation ability and lower cell apoptosis and senescence rates while maintaining the same osteogenetic differentiation ability as primary hPDLSCs. The transfection of hPDLSCs with lentivirus containing the TERT gene did not lead to tumorigenesis in nude mice. The Hippo signaling pathway was inactivated in TERT-hPDLSCs compared to hPDLSCs. When treated with verteporfin, the proliferation of TERT-hPDLSCs decreased, while the apoptosis and senescence rates of these cells increased. However, TERT-hPDLSCs still showed a stronger proliferation ability and lower cell apoptosis and senescence rates than hPDLSCs treated with verteporfin at the same concentration. Conclusions. Overexpression of TERT in hPDLSCs resulted in the successful establishment of an immortalized periodontal ligament stem cell line. TERT may regulate the biological characteristics of hPDLSCs through the Hippo/YAP signaling pathway. hPDLSCs could be a feasible resource for stem cell research and a promising resource for stem cell therapy.

A compound of concentrated growth factor and periodontal ligament stem cell-derived conditioned medium

2020 ◽  
Vol 65 ◽  
pp. 101373
Author(s):  
Z. Aghamohamadi ◽  
M. Kadkhodazadeh ◽  
M. Torshabi ◽  
F. Tabatabaei
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Conditioned Medium ◽  
Periodontal Ligament ◽  
Periodontal Ligament Stem Cell

Bioprinting 3D cell-laden hydrogel microarray for screening human periodontal ligament stem cell response to extracellular matrix

2015 ◽  
Vol 7 (4) ◽  
pp. 044105 ◽  
Author(s):  
Yufei Ma ◽  
Yuan Ji ◽  
Guoyou Huang ◽  
Kai Ling ◽  
Xiaohui Zhang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Cell Response ◽  
Periodontal Ligament ◽  
Periodontal Ligament Stem Cell

scholarly journals Osteocyte-derived exosomes induced by mechanical strain promote human periodontal ligament stem cell proliferation and osteogenic differentiation in an inflammatory environment via the miR-181b-5p/PTEN/AKT signaling pathway

2020 ◽  
Author(s):  
Peiying Lv ◽  
Pengfei Gao ◽  
Yanyan Yang ◽  
Zihui Wang ◽  
Lu Sun ◽  
...  
Keyword(s):  
Stem Cell ◽  
Osteogenic Differentiation ◽  
Periodontal Ligament ◽  
Mechanical Strain ◽  
Akt Signaling ◽  
Luciferase Reporter ◽  
Periodontal Tissue ◽  
Reporter Assay ◽  
Akt Signaling Pathway ◽  
Periodontal Ligament Stem Cell

Abstract Background: The oral cavity is a complex environment in which periodontal tissue is constantly stimulated by external microorganisms and mechanical forces. Proper mechanical force helps maintain periodontal tissue homeostasis and improper inflammatory response can break the balance. Periodontal ligament (PDL) cells plays crucial roles in responding these challenges and maintaining the homeostasis of periodontal tissue. However, the mechanisms underlying PDL cell property changes induced by inflammatory and mechanical force microenvironments are still unclear. Recent studies have shown that exosomes function as a mean of cell-cell and cell-matrix communication in biological processes. Methods: Human periodontal ligament stem cells (HPDLSCs) were tested by the CCK8 assay, EdU, alizarin red and ALP staining to evaluate the functions of exosomes induced by mechanical strain. MicroRNA sequencing was used to find the discrepancy miRNA in exosomes. In addition, RT-PCR, FISH, luciferase reporter assay and western blotting assay were used to investigated the mechanism of miR-181b-5p regulating proliferation and osteogenic differentiation through the PTEN/AKT pathway. Results: In this study, the exosomes secreted by MLO-Y4 cells exposed to mechanical strain (Exosome-MS) contributed to human periodontal ligament stem cell (HPDLSC) proliferation and osteogenic differentiation. High-throughput miRNA sequencing showed that miR181b-5p was upregulated in Exosome-MS compared to the exosomes derived from MLO-Y4 cells lacking MS. The luciferase reporter assay demonstrated that miR-181b-5p may target Phosphatase tension homolog deletion (PTEN). In addition, PTEN was negatively regulated by overexpressing miR-181b-5p. PCR and western blot analyses verified that miR‐181b-5p enhanced the protein kinase B (AKT) activity and improved downstream factor transcription. Furthermore, miR-181b-5p effectively ameliorated the inhibition of HPDLSC proliferation and osteogenesis induced by inflammation. Conclusions: This study concluded that exosomes induced by mechanical strain promote HPDLSC proliferation and osteogenic differentiation via the miR-181b-5p/PTEN/AKT signaling pathway, suggesting a potential mechanism for maintaining periodontal homeostasis.

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