scholarly journals The Effect of VPA on Increasing Radiosensitivity in Osteosarcoma Cells and Primary-Culture Cells from Chemical Carcinogen-Induced Breast Cancer in Rats

2017 ◽  
Vol 18 (5) ◽  
pp. 1027 ◽  
Author(s):  
Guochao Liu ◽  
Hui Wang ◽  
Fengmei Zhang ◽  
Youjia Tian ◽  
Zhujun Tian ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Primary Culture ◽  
Chemical Carcinogen ◽  
Osteosarcoma Cells ◽  
Culture Cells

scholarly journals Histochemical and electron microscopic study on primary culture cells from mucoepidermoid tumor of the oral region

1976 ◽  
Vol 22 (5) ◽  
pp. 631-638
Author(s):  
Masanao IWATA ◽  
Emiko YOKOO ◽  
Masatsugu SHIMIZU ◽  
Tadashi UENO ◽  
Gen-iku KOHAMA ◽  
...  
Keyword(s):  
Primary Culture ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Electron Microscopic ◽  
Oral Region ◽  
Culture Cells

scholarly journals Incorporation of 3H-thymidine and 3H-cytidine into Primary Culture Cells of the Corneal Epithelium

1962 ◽  
Vol 1962 (3) ◽  
pp. 136-140
Author(s):  
Takashi MIZUKAWA ◽  
Masakazu IGA ◽  
Jiro HARA ◽  
Shinichi OKUHARA ◽  
Toshifumi OTORI
Keyword(s):  
Primary Culture ◽  
Corneal Epithelium ◽  
Culture Cells

Abstract 1396: Response of primary culture of breast cancer to hypoxia and drug treatment.

2013 ◽  
Author(s):  
Hong Yin ◽  
Patrick Adegboyega ◽  
Mylinh Smith Smith ◽  
Jonathan Glass
Keyword(s):  
Breast Cancer ◽  
Drug Treatment ◽  
Primary Culture

2-Deoxy-D-glucose uptake by rat granular pneumocytes in primary culture

1982 ◽  
Vol 243 (1) ◽  
pp. C14-C19 ◽  
Author(s):  
J. S. Kerr ◽  
J. Reicherter ◽  
A. B. Fisher
Keyword(s):  
Glucose Uptake ◽  
Primary Culture ◽  
Free Form ◽  
Free Sugar ◽  
Type Ii ◽  
Minimal Essential Medium ◽  
External Concentration ◽  
Culture Cells ◽  
Carrier Mediated Transport ◽  
Sodium Dependent

Uptake of 2-deoxy-D-glucose (DG) was investigated with rat granular pneumocytes isolated in primary culture. Cells attached to flasks were incubated in Minimal Essential Medium usually containing 5 mM DG in place of glucose. Uptake of DG increased progressively with time of incubation and approached a plateau value of 35-40 mumol/10(6) cells at 60 min. Uptake increased as a function of external DG concentration with half-maximal uptake at approximately 2.0 mM DG. DG uptake was inhibited by the presence of glucose, alpha-methylglucoside, phlorizin, ouabain, or sodium-free medium. After 60 min incubation, approximately 20% of total intracellular DG was in the free form, and the calculated mean intracellular concentration of free DG (n = 4) was approximately twice the external concentration. Phosphatase activity was indicated by increase in free DG and efflux from cells after removal of external DG. In comparison with pneumocytes, uptake of DG by alveolar macrophages showed different kinetics, and intracellular free DG did not exceed the extracellular concentration. These findings indicate that type II cells take up DG by a sodium-dependent, carrier-mediated transport process that results in accumulation of free sugar against a concentration gradient.

An apparatus to facilitate the preparation of primary culture cells

1963 ◽  
Vol 12 (5) ◽  
pp. 672-677 ◽  
Author(s):  
G. H. Bergold ◽  
H. Herburger
Keyword(s):  
Primary Culture ◽  
Culture Cells

scholarly journals Generation and Characterization of an IGFBP-7 Antibody: Identification of 31kD IGFBP-7 in Human Biological Fluids and Hs578T Human Breast Cancer Conditioned Media

1997 ◽  
Vol 82 (4) ◽  
pp. 1301-1303 ◽  
Author(s):  
Elizabeth M. Wilson ◽  
Youngman Oh ◽  
Ron G. Rosenfeld
Keyword(s):  
Breast Cancer ◽  
Mammary Epithelial Cells ◽  
Polyclonal Antibodies ◽  
Biological Fluids ◽  
Physiological Role ◽  
Cellular Level ◽  
Conditioned Media ◽  
Igf Binding Proteins ◽  
Culture Cells

Abstract The cDNA encoding mac25 (IGFBP-7) was first derived from mRNA isolated from leptomeningial and senescent human mammary epithelial cells (1, 2). The open reading frame was shown to predict a protein with homology to the amino terminus of the IGF binding proteins, (IGFBP)1-6. Studies in our laboratory have shown that baculovirus generated mac25 binds IGF-I and-II in a specific manner, leading to the renaming of mac25 as IGFBP-7 (3). Further studies at the cellular level, to identify the involvement of IGFBP-7 in IGF regulation and cell growth, require a specific antibody against the protein, which has yet to be identified in either culture cells or in vivo. We have now generated three polyclonal antibodies against the purified baculovirus peptide, and, by western immunoblots and immunoprecipitation, demonstrated the existence of a specific 31,000 dalton protein. It is a secreted protein and can be identified in the conditioned media of Hs578T breast cancer cells, as well as in normal human urine, cerebrospinal fluid, and amniotic fluid. Subsequent studies with these antibodies should help elucidate the physiological role(s) of this protein.

scholarly journals Effect of the Anti-Breast Cancer Drug Tamoxifen on Ca2+ Movement in Human Osteosarcoma Cells

2002 ◽  
Vol 91 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Yih-Chau Lu ◽  
Bang-Ping Jiann ◽  
Hong-Tai Chang ◽  
Jong-Khing Huang ◽  
Wei-Chung Chen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Drug ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

scholarly journals MARCADORES TUMORALES PRONÓSTICOS EN CÁNCER DE MAMA: BAX y Bcl-2

2022 ◽  
Vol 7 (1) ◽  
pp. 24
Author(s):  
Lizette Elena Leiva Suero ◽  
Yenddy Carrero Castillo ◽  
Graciela de las Mercedes Quishpe Jara ◽  
Sandra Villacís Valencia ◽  
Jesús Onorato Chicaiza Tayupanta ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Breast Carcinoma ◽  
Tumor Markers ◽  
Inhibitory Effect ◽  
Cáncer De Mama ◽  
Cell Models ◽  
Culture Cells ◽  
Carcinoma De Mama ◽  
Marcadores Tumorales ◽  
Mcf 7

Introducción: El carcinoma de mama representa el 16% de todos los cánceres femeninos, cada año en las Américas, más de 462,000 mujeres son diagnosticadas y casi 100,000 mueren por esta causa, por lo que la detección de nuevos marcadores pronósticos se hace imprescindible para el desarrollo de nuevas terapias oncológicas. Objetivo: Identificar los marcadores tumorales con fines pronósticos en cáncer de mama en células de cultivo de carcinoma de mama MCF7. Método: Se desarrolló un estudio explicativo longitudinal y prospectivo observacional para identificar marcadores tumorales con valor pronóstico en modelos celulares MCF7 de Cáncer de Mama, se utilizarán métodos estadísticos descriptivos e inferenciales. Resultados: Se identificó un predominio de Bcl-2 en las células de cultivo MCF7 de Carcinoma de Mama. Conclusión: La identificación de BAX y Bcl-2 en modelos celulares MCF 7 de Carcinoma de Mama permite evaluar nuevas opciones terapéuticas relacionadas con la inducción de la apoptosis. La predominancia Bcl-2 pudiera constituir un factor de mal pronóstico y menor supervivencia debido a su efecto inhibidor de la apoptosis, donde subyacen alteraciones mitocondriales, que inciden en la regulación del nivel de calcio intracelular, o por su efecto antioxidante.   Palabras clave: Neoplasias de la mama, Pronóstico, Apoptosis, Proteína de Susceptibilidad a Apoptosis Celular   ABSTRACT    Introduction: Breast carcinoma represents 16% of all female cancers, each year in the Americas, more than 462,000 women are diagnosed and almost 100,000 die from this cause, so the detection of new prognostic markers is essential for the development of new oncological therapies. Objective: To identify tumor markers for prognostic purposes in breast cancer in MCF7 breast carcinoma culture cells. Method: A longitudinal and prospective observational explanatory study was developed to identify tumor markers with prognostic value in MCF7 cell models of Breast Cancer, descriptive and inferential statistical methods will be used. Results: A predominance of Bcl-2 was identified in the MCF7 culture cells of Breast Carcinoma. Conclusion: The identification of BAX and Bcl-2 in MCF 7 cell models of Breast Carcinoma allows to evaluate new therapeutic options related to the induction of apoptosis. The Predominance bcl-2 could constitute a factor of poor prognosis and lower survival due to its inhibitory effect of apoptosis, where mitochondrial alterations underlie, which affect the regulation of the level of intracellular calcium, or for its antioxidant effect. Keywords: Breast Neoplasms, Prognosis, Apoptosis, Cellular Apoptosis Susceptibility Protein

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