An apparatus to facilitate the preparation of primary culture cells

1963 ◽  
Vol 12 (5) ◽  
pp. 672-677 ◽  
Author(s):  
G. H. Bergold ◽  
H. Herburger
Keyword(s):  
Primary Culture ◽  
Culture Cells

scholarly journals Histochemical and electron microscopic study on primary culture cells from mucoepidermoid tumor of the oral region

1976 ◽  
Vol 22 (5) ◽  
pp. 631-638
Author(s):  
Masanao IWATA ◽  
Emiko YOKOO ◽  
Masatsugu SHIMIZU ◽  
Tadashi UENO ◽  
Gen-iku KOHAMA ◽  
...  
Keyword(s):  
Primary Culture ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Electron Microscopic ◽  
Oral Region ◽  
Culture Cells

scholarly journals Incorporation of 3H-thymidine and 3H-cytidine into Primary Culture Cells of the Corneal Epithelium

1962 ◽  
Vol 1962 (3) ◽  
pp. 136-140
Author(s):  
Takashi MIZUKAWA ◽  
Masakazu IGA ◽  
Jiro HARA ◽  
Shinichi OKUHARA ◽  
Toshifumi OTORI
Keyword(s):  
Primary Culture ◽  
Corneal Epithelium ◽  
Culture Cells

2-Deoxy-D-glucose uptake by rat granular pneumocytes in primary culture

1982 ◽  
Vol 243 (1) ◽  
pp. C14-C19 ◽  
Author(s):  
J. S. Kerr ◽  
J. Reicherter ◽  
A. B. Fisher
Keyword(s):  
Glucose Uptake ◽  
Primary Culture ◽  
Free Form ◽  
Free Sugar ◽  
Type Ii ◽  
Minimal Essential Medium ◽  
External Concentration ◽  
Culture Cells ◽  
Carrier Mediated Transport ◽  
Sodium Dependent

Uptake of 2-deoxy-D-glucose (DG) was investigated with rat granular pneumocytes isolated in primary culture. Cells attached to flasks were incubated in Minimal Essential Medium usually containing 5 mM DG in place of glucose. Uptake of DG increased progressively with time of incubation and approached a plateau value of 35-40 mumol/10(6) cells at 60 min. Uptake increased as a function of external DG concentration with half-maximal uptake at approximately 2.0 mM DG. DG uptake was inhibited by the presence of glucose, alpha-methylglucoside, phlorizin, ouabain, or sodium-free medium. After 60 min incubation, approximately 20% of total intracellular DG was in the free form, and the calculated mean intracellular concentration of free DG (n = 4) was approximately twice the external concentration. Phosphatase activity was indicated by increase in free DG and efflux from cells after removal of external DG. In comparison with pneumocytes, uptake of DG by alveolar macrophages showed different kinetics, and intracellular free DG did not exceed the extracellular concentration. These findings indicate that type II cells take up DG by a sodium-dependent, carrier-mediated transport process that results in accumulation of free sugar against a concentration gradient.

Expression and function of laminin and integrins on adhesion/migration of primary culture cells derived from rat oral epithelium

2010 ◽  
Vol 45 (2) ◽  
pp. 284-291 ◽  
Author(s):  
M. Sugisawa ◽  
T. Masaoka ◽  
Y. Enokiya ◽  
T. Muramatsu ◽  
S. Hashimoto ◽  
...  
Keyword(s):  
Primary Culture ◽  
Oral Epithelium ◽  
Culture Cells ◽  
And Function
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