scholarly journals Combining Low Temperature Fluorescence DNA-Hybridization, Immunostaining, and Super-Resolution Localization Microscopy for Nano-Structure Analysis of ALU Elements and Their Influence on Chromatin Structure

2017 ◽  
Vol 18 (5) ◽  
pp. 1005 ◽  
Author(s):  
Matthias Krufczik ◽  
Aaron Sievers ◽  
Annkathrin Hausmann ◽  
Jin-Ho Lee ◽  
Georg Hildenbrand ◽  
...  
Keyword(s):  
Low Temperature ◽  
Structure Analysis ◽  
Chromatin Structure ◽  
Dna Hybridization ◽  
Super Resolution ◽  
Nano Structure ◽  
Alu Elements ◽  
Localization Microscopy

scholarly journals Comparing Super-Resolution Microscopy Techniques to Analyze Chromosomes

2021 ◽  
Vol 22 (4) ◽  
pp. 1903
Author(s):  
Ivona Kubalová ◽  
Alžběta Němečková ◽  
Klaus Weisshart ◽  
Eva Hřibová ◽  
Veit Schubert
Keyword(s):  
Single Molecule ◽  
Imaging Techniques ◽  
Chromatin Condensation ◽  
Super Resolution ◽  
Stimulated Emission ◽  
Wide Field ◽  
Structured Illumination Microscopy ◽  
Metaphase Chromosomes ◽  
Localization Microscopy ◽  
Super Resolution Microscopy

The importance of fluorescence light microscopy for understanding cellular and sub-cellular structures and functions is undeniable. However, the resolution is limited by light diffraction (~200–250 nm laterally, ~500–700 nm axially). Meanwhile, super-resolution microscopy, such as structured illumination microscopy (SIM), is being applied more and more to overcome this restriction. Instead, super-resolution by stimulated emission depletion (STED) microscopy achieving a resolution of ~50 nm laterally and ~130 nm axially has not yet frequently been applied in plant cell research due to the required specific sample preparation and stable dye staining. Single-molecule localization microscopy (SMLM) including photoactivated localization microscopy (PALM) has not yet been widely used, although this nanoscopic technique allows even the detection of single molecules. In this study, we compared protein imaging within metaphase chromosomes of barley via conventional wide-field and confocal microscopy, and the sub-diffraction methods SIM, STED, and SMLM. The chromosomes were labeled by DAPI (4′,6-diamidino-2-phenylindol), a DNA-specific dye, and with antibodies against topoisomerase IIα (Topo II), a protein important for correct chromatin condensation. Compared to the diffraction-limited methods, the combination of the three different super-resolution imaging techniques delivered tremendous additional insights into the plant chromosome architecture through the achieved increased resolution.

scholarly journals Camera-based localization microscopy optimized with calibrated structured illumination

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Martin Schmidt ◽  
Adam C. Hundahl ◽  
Henrik Flyvbjerg ◽  
Rodolphe Marie ◽  
Kim I. Mortensen
Keyword(s):  
Data Analysis ◽  
Super Resolution ◽  
Wide Field ◽  
Structured Illumination ◽  
Uniform Illumination ◽  
Localization Microscopy ◽  
Localization And Tracking ◽  
Field Imaging ◽  
Wide Field Imaging ◽  
Localization Information

AbstractUntil very recently, super-resolution localization and tracking of fluorescent particles used camera-based wide-field imaging with uniform illumination. Then it was demonstrated that structured illuminations encode additional localization information in images. The first demonstration of this uses scanning and hence suffers from limited throughput. This limitation was mitigated by fusing camera-based localization with wide-field structured illumination. Current implementations, however, use effectively only half the localization information that they encode in images. Here we demonstrate how all of this information may be exploited by careful calibration of the structured illumination. Our approach achieves maximal resolution for given structured illumination, has a simple data analysis, and applies to any structured illumination in principle. We demonstrate this with an only slightly modified wide-field microscope. Our protocol should boost the emerging field of high-precision localization with structured illumination.

scholarly journals Characterization of Locus Specific Chromatin Structure and Dynamics using Correlative Conventional Super Resolution Crispr dCas9/Ms2 Imaging

2021 ◽  
Vol 120 (3) ◽  
pp. 9a
Author(s):  
Dushyant Mehra ◽  
Chiranjib Banerjee ◽  
Santosh Adhikari ◽  
Jacob M. Ritz ◽  
Angel Mancebo ◽  
...  
Keyword(s):  
Chromatin Structure ◽  
Super Resolution ◽  
Structure And Dynamics ◽  
Chromatin Structure And Dynamics

Comparison of the NMR coupling paths in leucodrin and conocarpin by low-temperature X-ray structure analysis

1991 ◽  
pp. 695 ◽  
Author(s):  
D. G. Billing ◽  
J. C. A. Boeyens ◽  
L. Carlton ◽  
D. C. Levendis ◽  
G. W. Perold
Keyword(s):  
Low Temperature ◽  
Structure Analysis ◽  
X Ray

scholarly journals Optimizing Imaging Conditions for Demanding Multi-Color Super Resolution Localization Microscopy

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0158884 ◽  
Author(s):  
Leila Nahidiazar ◽  
Alexandra V. Agronskaia ◽  
Jorrit Broertjes ◽  
Bram van den Broek ◽  
Kees Jalink
Keyword(s):  
Super Resolution ◽  
Localization Microscopy ◽  
Imaging Conditions

Low-Temperature Liquid-Phase Synthesis and Electrochemical Activity of α-Nickel Hydroxide with Flower-Like Micro-/Nano-Structure

2011 ◽  
Vol 347-353 ◽  
pp. 3379-3383
Author(s):  
Zhi Wei Li ◽  
Xu Xiang ◽  
Zong Min Tian
Keyword(s):  
Liquid Phase ◽  
Low Temperature ◽  
Ethylene Glycol ◽  
Nickel Hydroxide ◽  
Variable Temperature ◽  
Mixed Solvents ◽  
Reaction Medium ◽  
Electrochemical Activity ◽  
Nano Structure ◽  
Pure Phase

The synthesis of α-nickel hydroxide has been achieved via a facile liquid-phase precipitation approach, using the mixed solvents of ethylene glycol and water as reaction medium at low temperature. The XRD characterization indicates that pure phase α-Ni(OH)2can be obtained under variable temperature and pH value. The products present a flower-like micro-/nano-structure assembled with curved nanosheets. The nanosheets have the width of 100~500 nm and the thickness of 20~70 nm. The cavities are formed in the structure due to the interconnection of curved nanosheets. The solvents play a key role in the formation of Ni(OH)2with different forms. Pure phase α-Ni(OH)2can only be synthesized in the mixed solvents of ethylene glycol and water. Cyclic voltammetry was applied to test the electrochemical activity of the as-synthesized α-Ni(OH)2. The findings suggest that the α-Ni(OH)2with a micro-/nano-structure exhibits excellent electrochemical activity, which may be considered as a promising candidate of electrode material.

scholarly journals Super-resolution binding activated localization microscopy through reversible change of DNA conformation

Nucleus ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 182-189 ◽  
Author(s):  
Aleksander Szczurek ◽  
Udo Birk ◽  
Hans Knecht ◽  
Jurek Dobrucki ◽  
Sabine Mai ◽  
...  
Keyword(s):  
Super Resolution ◽  
Dna Conformation ◽  
Reversible Change ◽  
Localization Microscopy

Aberration Characterization and Correction in Super-Resolution Localization Microscopy

2017 ◽  
Vol 37 (3) ◽  
pp. 0318004
Author(s):  
赵泽宇 Zhao Zeyu ◽  
张肇宁 Zhang Zhaoning ◽  
黄振立 Huang Zhenli
Keyword(s):  
Super Resolution ◽  
Localization Microscopy
Sign in / Sign up

Export Citation Format

Share Document