scholarly journals Cultivar-Specific Changes in Primary and Secondary Metabolites in Pak Choi (Brassica Rapa, Chinensis Group) by Methyl Jasmonate

2017 ◽  
Vol 18 (5) ◽  
pp. 1004 ◽  
Author(s):  
Moo Kim ◽  
Yu-Chun Chiu ◽  
Na Kim ◽  
Hye Park ◽  
Choong Lee ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Methyl Jasmonate ◽  
Brassica Rapa ◽  
Pak Choi ◽  
Primary And Secondary Metabolites

scholarly journals Metabolomic analysis reveals the interaction of primary and secondary metabolism in white, pale green, and green pak choi (Brassica rapa subsp. chinensis)

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Hyeon Ji Yeo ◽  
Seung-A Baek ◽  
Ramaraj Sathasivam ◽  
Jae Kwang Kim ◽  
Sang Un Park
Keyword(s):  
Secondary Metabolites ◽  
Brassica Rapa ◽  
High Performance ◽  
Reducing Power ◽  
Total Phenolic ◽  
Pak Choi ◽  
Primary And Secondary Metabolites ◽  
Chlorophyll Contents ◽  
Significant Difference ◽  
Pale Green

AbstractThis study aimed to comprehensively analyze primary and secondary metabolites of three different-colored (white, pale green, and green) pak choi cultivars (Brassica rapa subsp. chinensis) using gas chromatography attached with time-of-flight mass spectrometry (GC-TOFMS) and high-performance liquid chromatography (HPLC). In total, 53 primary metabolites were identified and subjected to partial least-squares discriminant analysis. The result revealed a significant difference in the primary and secondary metabolites between the three pak choi cultivars. In addition, 49 hydrophilic metabolites were detected in different cultivars. Total phenolic and glucosinolate contents were highest in the pale green and green cultivars, respectively, whereas total carotenoid and chlorophyll contents were highest in the white cultivar. Superoxide dismutase activity, 2,2-diphenyl-1-picrylhydraz scavenging, and reducing power were slightly increased in the white, pale green, and green cultivars, respectively. In addition, a negative correlation between pigments and phenylpropanoids was discovered by metabolite correlation analysis. This approach will provide useful information for the development of strategies to enhance the biosynthesis of phenolics, glucosinolates, carotenoids, and chlorophyll, and to improve antioxidant activity in pak choi cultivars. In addition, this study supports the use of HPLC and GC-TOFMS-based metabolite profiling to explore differences in pak choi cultivars.

scholarly journals Boiling and steaming induced changes in secondary metabolites in three different cultivars of pak choi (Brassica rapa subsp. chinensis)

2019 ◽  
Vol 82 ◽  
pp. 103232 ◽  
Author(s):  
Xiaomin Chen ◽  
Franziska S. Hanschen ◽  
Susanne Neugart ◽  
Monika Schreiner ◽  
Sara A. Vargas ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Brassica Rapa ◽  
Pak Choi ◽  
Induced Changes

Glucosinolate enhancement in leaves and roots of pak choi (Brassica rapa ssp. chinensis) by methyl jasmonate

2015 ◽  
Vol 56 (6) ◽  
pp. 830-840 ◽  
Author(s):  
Yunxiang Zang ◽  
Hao Zhang ◽  
Linghui Huang ◽  
Fang Wang ◽  
Fei Gao ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Brassica Rapa ◽  
Pak Choi ◽  
Leaves And Roots

scholarly journals Comparative Phytochemical Analyses and Metabolic Profiling of Different Phenotypes of Chinese Cabbage (Brassica Rapa ssp. Pekinensis)

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 587 ◽  
Author(s):  
Chang Ha Park ◽  
Hyeon Ji Yeo ◽  
Soo-Yun Park ◽  
Jae Kwang Kim ◽  
Sang Un Park
Keyword(s):  
Secondary Metabolites ◽  
Brassica Rapa ◽  
Chinese Cabbage ◽  
High Performance ◽  
Total Carotenoids ◽  
Primary Metabolites ◽  
Primary And Secondary Metabolites ◽  
Phenolic Contents ◽  
Flight Mass Spectrometry ◽  
The Relationship

This study aimed to comprehensively examine the interface between primary and secondary metabolites in oval- and rectangular-shaped Chinese cabbage (Brassica rapa ssp. pekinensis) using gas chromatography coupled with time-of-flight mass spectrometry (GC-TOFMS) and high-performance liquid chromatography (HPLC). In addition to differences in shape, there was significant morphological variation between the two cultivars. The rectangular variety had greater height and deeper green color, whereas the oval variety had more leaves and greater width. A total of 42 primary metabolites identified by GC-TOFMS were subjected to partial least-squares discriminant, which indicated significant differences in the primary and secondary metabolisms of the two cultivars. Furthermore, total glucosinolate and phenolic contents were higher in the oval cultivar, whereas the rectangular cultivar contained a higher level of total carotenoids. This metabolome study comprehensively describes the relationship between primary and secondary metabolites in the oval and rectangular cultivars of Chinese cabbage and provides information useful for developing strategies to enhance the biosynthesis of glucosinolates, phenolics, and carotenoids in Chinese cabbage. Additionally, this work highlights that HPLC and GC-TOFMS–based metabolite profiling is suitable techniques to determine metabolic differences in Chinese cabbage.

scholarly journals Induced Production of 1-Methoxy-indol-3-ylmethyl Glucosinolate by Jasmonic Acid and Methyl Jasmonate in Sprouts and Leaves of Pak Choi (Brassica rapa ssp. chinensis)

2013 ◽  
Vol 14 (7) ◽  
pp. 14996-15016 ◽  
Author(s):  
Melanie Wiesner ◽  
Franziska Hanschen ◽  
Monika Schreiner ◽  
Hansruedi Glatt ◽  
Rita Zrenner
Keyword(s):  
Jasmonic Acid ◽  
Methyl Jasmonate ◽  
Brassica Rapa ◽  
Pak Choi

Comparison Among Five Eucalyptus Species Based on Their Leaf Contents of Some Primary and Secondary Metabolites

2019 ◽  
Vol 20 (7) ◽  
pp. 573-587
Author(s):  
Alyaa Nasr ◽  
Tehmina Saleem Khan ◽  
Shi-Ping Huang ◽  
Bin Wen ◽  
Jian-Wen Shao ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Protocatechuic Acid ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Renewable Resource ◽  
Total Phenolic ◽  
Eucalyptus Species ◽  
Primary And Secondary Metabolites ◽  
Acetone Water ◽  
Phenolic Constituents

Background: Eucalyptus belongs to the Myrtaceae family. It is the most planted hardwood forest crop worldwide, representing a global renewable resource of fiber, pharmaceuticals and energy. Objective: To compare the five species, E. maidenii, E. robusta, E. citriodora, E. tereticornis and E. camaldulensis, seeking for the richest source of nutrients and pharmaceuticals. Methodology: Eucalyptus samples were subjected to some chemical determinations for both primary and secondary metabolites to verify their nutritional and pharmaceutical importance related to different extracts. GC-MS analysis was applied to detect the presence of some individual phenolic constituents in their leaves. Results: E. robusta recorded the maximum contents of carbohydrates (40.07%) and protein (31.91%). While E. camaldulensis contained the highest contents of total phenolic compounds (46.56 mg/g), tannins (40.01 mg/g) and antioxidant activities assayed by the phosphomolybednum method (57.60 mg/g), followed by E. citridora. However, E. tereticornis exhibited the highest reducing power ability (151.23 mg/g). The GC-MS highlighted 20 phenolic constituents and antioxidants which varied in their abundance in Eucalyptus leaves, 8 individual phenolics (hydroquinone, hesperitin, pyrogallol, resorcinol, protocatechuic acid, naringenin, chlorogenic acid and catechin) were maximally recorded with E. camaldulensis and secondly, with E. citridora in case of at least 5 components. Nevertheless, gallic and quinic acids were more abundant in the leaves of E. tereticornis, which may explain its high corresponding reducing powers. Conclusion: Acetone-water combination has enhanced phenolics extraction from Eucalyptus tissues. This is the first report aiming to compare between the aforementioned Eucalyptus species highlighting either their nutritional or medicinal importance.

scholarly journals Profiles of Secondary Metabolites (Phenolic Acids, Carotenoids, Anthocyanins, and Galantamine) and Primary Metabolites (Carbohydrates, Amino Acids, and Organic Acids) during Flower Development in Lycoris radiata

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 248
Author(s):  
Chang Ha Park ◽  
Hyeon Ji Yeo ◽  
Ye Jin Kim ◽  
Bao Van Nguyen ◽  
Ye Eun Park ◽  
...  
Keyword(s):  
Amino Acids ◽  
Secondary Metabolites ◽  
Organic Acids ◽  
Phenolic Acids ◽  
Flower Development ◽  
Developmental Stages ◽  
Tca Cycle ◽  
Primary And Secondary Metabolites ◽  
Hydrophilic Compounds ◽  
Tca Cycle Intermediates

This study aimed to elucidate the variations in primary and secondary metabolites during Lycorisradiata flower development using high performance liquid chromatography (HPLC) and gas chromatography time-of-flight mass spectrometry (GC-TOFMS). The result showed that seven carotenoids, seven phenolic acids, three anthocyanins, and galantamine were identified in the L. radiata flowers. Most secondary metabolite levels gradually decreased according to the flower developmental stages. A total of 51 metabolites, including amines, sugars, sugar intermediates, sugar alcohols, amino acids, organic acids, phenolic acids, and tricarboxylic acid (TCA) cycle intermediates, were identified and quantified using GC-TOFMS. Among the hydrophilic compounds, most amino acids increased during flower development; in contrast, TCA cycle intermediates and sugars decreased. In particular, glutamine, asparagine, glutamic acid, and aspartic acid, which represent the main inter- and intracellular nitrogen carriers, were positively correlated with the other amino acids and were negatively correlated with the TCA cycle intermediates. Furthermore, quantitation data of the 51 hydrophilic compounds were subjected to partial least-squares discriminant analyses (PLS-DA) to assess significant differences in the metabolites of L. radiata flowers from stages 1 to 4. Therefore, this study will serve as the foundation for a biochemical approach to understand both primary and secondary metabolism in L. radiata flower development.

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