scholarly journals In Vitro Investigation of Self-Assembled Nanoparticles Based on Hyaluronic Acid-Deoxycholic Acid Conjugates for Controlled Release Doxorubicin: Effect of Degree of Substitution of Deoxycholic Acid

2015 ◽  
Vol 16 (12) ◽  
pp. 7195-7209 ◽  
Author(s):  
Wen-Hao Wei ◽  
Xue-Meng Dong ◽  
Chen-Guang Liu
Keyword(s):  
Hyaluronic Acid ◽  
Controlled Release ◽  
Deoxycholic Acid ◽  
Degree Of Substitution ◽  
In Vitro Investigation ◽  
Self Assembled

Redox-sensitive micelles self-assembled from amphiphilic hyaluronic acid-deoxycholic acid conjugates for targeted intracellular delivery of paclitaxel

Biomaterials ◽  
2012 ◽  
Vol 33 (7) ◽  
pp. 2310-2320 ◽  
Author(s):  
Jing Li ◽  
Meirong Huo ◽  
Jing Wang ◽  
Jianping Zhou ◽  
Jumah M. Mohammad ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Deoxycholic Acid ◽  
Intracellular Delivery ◽  
Self Assembled

scholarly journals Preparation and Characterization of Self-Assembled Nanoparticles of Hyaluronic Acid-Deoxycholic Acid Conjugates

2010 ◽  
Vol 2010 ◽  
pp. 1-9 ◽  
Author(s):  
Xuemeng Dong ◽  
Chenguang Liu
Keyword(s):  
Hyaluronic Acid ◽  
Deoxycholic Acid ◽  
Structural Characteristics ◽  
Cetylpyridinium Chloride ◽  
Coupling Reaction ◽  
Critical Aggregation Concentration ◽  
Transmission Electron ◽  
Fluorescence Quenching Method ◽  
Self Assembled ◽  
Quenching Method

Novel amphiphilic biopolymers were synthesized using hyaluronic acid (HA) as a hydrophilic segment and deoxycholic acid (DOCA) as a hydrophobic segment by a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide mediated coupling reaction. The structural characteristics of the HA-DOCA conjugates were investigated usingH1NMR. Self-assembled nanoparticles were prepared based on HA-DOCA conjugates, and its characteristics were investigated using dynamic laser light scattering, transmission electron microscopy (TEM), and fluorescence spectroscopy. The mean diameter was about 293.5 nm with unimodal size distribution in distilled water. The TEM images revealed that the shape of HA-DOCA self-aggregates was spherical. The critical aggregation concentration (CAC) was in the range of 0.025–0.056 mg/mL. The partition equilibrium constant (Kv) of pyrene in self-aggregates solution was from1.45×104to3.64×104. The aggregation number of DOCA groups per hydrophobic microdomain, estimated by the fluorescence quenching method using cetylpyridinium chloride, increased with increasing degree of substitution.

scholarly journals APTES monolayer coverage on self-assembled magnetic nanospheres for controlled release of anticancer drug Nintedanib

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
V. C. Karade ◽  
A. Sharma ◽  
R. P. Dhavale ◽  
R. P. Dhavale ◽  
S. R. Shingte ◽  
...  
Keyword(s):  
Controlled Release ◽  
Photoelectron Spectroscopy ◽  
Superparamagnetic Iron Oxide ◽  
In Vitro Cytotoxicity ◽  
Human Lung Cancer ◽  
Prolonged Release ◽  
Magnetic Nanospheres ◽  
Self Assembled ◽  
Monolayer Coverage

AbstractThe use of an appropriate delivery system capable of protecting, translocating, and selectively releasing therapeutic moieties to desired sites can promote the efficacy of an active compound. In this work, we have developed a nanoformulation which preserves its magnetization to load a model anticancerous drug and to explore the controlled release of the drug in a cancerous environment. For the preparation of the nanoformulation, self-assembled magnetic nanospheres (MNS) made of superparamagnetic iron oxide nanoparticles were grafted with a monolayer of (3-aminopropyl)triethoxysilane (APTES). A direct functionalization strategy was used to avoid the loss of the MNS magnetization. The successful preparation of the nanoformulation was validated by structural, microstructural, and magnetic investigations. X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR) were used to establish the presence of APTES on the MNS surface. The amine content quantified by a ninhydrin assay revealed the monolayer coverage of APTES over MNS. The monolayer coverage of APTES reduced only negligibly the saturation magnetization from 77 emu/g (for MNS) to 74 emu/g (for MNS-APTES). Detailed investigations of the thermoremanent magnetization were carried out to assess the superparamagnetism in the MNS. To make the nanoformulation pH-responsive, the anticancerous drug Nintedanib (NTD) was conjugated with MNS-APTES through the acid liable imine bond. At pH 5.5, which mimics a cancerous environment, a controlled release of 85% in 48 h was observed. On the other hand, prolonged release of NTD was found at physiological conditions (i.e., pH 7.4). In vitro cytotoxicity study showed dose-dependent activity of MNS-APTES-NTD for human lung cancer cells L-132. About 75% reduction in cellular viability for a 100 μg/mL concentration of nanoformulation was observed. The nanoformulation designed using MNS and monolayer coverage of APTES has potential in cancer therapy as well as in other nanobiological applications.

In vitro investigation of lipid implants as a controlled release system for interleukin-18

2006 ◽  
Vol 314 (2) ◽  
pp. 145-152 ◽  
Author(s):  
S. Koennings ◽  
E. Garcion ◽  
N. Faisant ◽  
P. Menei ◽  
J.P. Benoit ◽  
...  
Keyword(s):  
Controlled Release ◽  
Interleukin 18 ◽  
Release System ◽  
In Vitro Investigation ◽  
Controlled Release System
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