scholarly journals Phenological, Nutritional and Molecular Diversity Assessment among 35 Introduced Lentil (Lens culinaris Medik.) Genotypes Grown in Saudi Arabia

2013 ◽  
Vol 15 (1) ◽  
pp. 277-295 ◽  
Author(s):  
Salem Alghamdi ◽  
Altaf Khan ◽  
Megahed Ammar ◽  
Ehab El-Harty ◽  
Hussein Migdadi ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Lens Culinaris ◽  
Molecular Diversity ◽  
Diversity Assessment

scholarly journals SSR DNA marker aided genetic diversity assessment of selected willow clones

Genetika ◽  
2013 ◽  
Vol 45 (2) ◽  
pp. 527-536 ◽  
Author(s):  
N.B. Singh ◽  
S. Joshi ◽  
P. Choudhary ◽  
J.P. Sharma
Keyword(s):  
Genetic Diversity ◽  
Molecular Diversity ◽  
Dna Marker ◽  
Similarity Coefficient ◽  
Gene Pools ◽  
Morphometric Traits ◽  
Genetic Base ◽  
Diversity Assessment ◽  
Ssr Primers ◽  
Clustering Pattern

Around 100 clones of tree willows were subjected for nursery screening twice on morphometric traits. Genetic diversity was assessed in twenty-five genetically superior willow clones hailing from six countries using 16 SSR primers. Fourteen primers amplified the DNA but only ten showed polymorphism. Total 34 bands were scored, out of that 27 were found to be polymorphic and 7 were monomorphic. Three primers showed 100% polymorphism whereas 79.4% polymorphism was recorded in total. The dendrogram obtained from SSR markers revealed that clone SE-69-002 (S. matsudana) and NZ-1040 (S. matsudana X S. alba) as most similar clones (Jaccards coefficient of 0.97), and clone PN-721(S. matsudana X S. alba) and PN-731 (S. nigra), as most divergent clones (Jaccards coefficient of 0.63). All the genotypes were grouped into 4 distinct clusters. On the basis of similarity coefficient analysis the first cluster comprised of 11 genotypes, the second cluster have 8 genotypes where as third one has only one genotype and fourth cluster retained five genotypes. The clustering pattern further indicated that the geographic distribution may not be the reflection of genetic diversity in willow clones. Genotypes with high molecular diversity could be used in breeding programme in order to obtain heterotic hybrids and development of gene pools with broad genetic base. The genotype specific bands developed by the SSR primers could also be used for identification of cultivar.

scholarly journals Isolation, identification, and molecular diversity of indigenous isolates of Beauveria bassiana from Taif region, Saudi Arabia

2018 ◽  
Vol 28 (1) ◽  
Author(s):  
Samy Mahmoud Sayed ◽  
Esmat Farouk Ali ◽  
Sayed Ashraf El-Arnaouty ◽  
Samy Farouk Mahmoud ◽  
Sayed Amin Amer
Keyword(s):  
Saudi Arabia ◽  
Beauveria Bassiana ◽  
Molecular Diversity

scholarly journals Molecular diversity assessment of AVRDC–The World Vegetable Center elite-parental mungbeans

2009 ◽  
Vol 59 (2) ◽  
pp. 149-157 ◽  
Author(s):  
Prakit Somta ◽  
Warunee Sommanas ◽  
Peerasak Srinives
Keyword(s):  
Molecular Diversity ◽  
Diversity Assessment ◽  
The World

scholarly journals Molecular Diversity Assessment of PGPR Using Enterobacterial Repetitive Intergeneric Consensus (ERIC) PCR

2018 ◽  
Vol 7 ◽  
pp. 64-70
Author(s):  
Umesh Prasad Shrivastava
Keyword(s):  
Plant Growth ◽  
Molecular Diversity ◽  
Protein Profile ◽  
Plant Growth Promoting Rhizobacteria ◽  
Rice Fields ◽  
Border Region ◽  
Diversity Assessment ◽  
Plant Growth Promoting ◽  
Growth Promoting

In the present investigation, an attempt has been made to study the diversity of Plant Growth Promoting Rhizobacteria isolated from various rice fields of Indo-Nepal border region. It is reported here that diversity in PGPR of East Champaran, West Champaran and Varanasi district of India and Bara and Parsa district of Nepal showed 76 to 97% similarity among them based on ERIC PCR. Serratia, Agrobacterium, Klebsiella, Pseudomonas etc are predominantly present in the rhizosphere of rice fields of these areas. Protein profile showed that all the isolates were 49-79% similar among each other. The three members consisting species of Klebsiella showed 72% similarity to each other and two species of Agrobacterium showed 80% similarity to each other.

MOLECULAR DIVERSITY ASSESSMENT OF CUMIN ECOTYPES USING AFLP MARKERS

2014 ◽  
pp. 263-270
Author(s):  
A. Bahraminejad ◽  
G. Mohammadinejad ◽  
M. Abdul Kadir
Keyword(s):  
Molecular Diversity ◽  
Aflp Markers ◽  
Diversity Assessment

scholarly journals Genetic Diversity Assessment in Pea (Pisum sativum L.) using Microsatellite Markers

2021 ◽  
Vol 12 (5) ◽  
pp. 402-408
Author(s):  
Hanuman Ram ◽  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Molecular Diversity ◽  
Polymorphic Information Content ◽  
Association Studies ◽  
Pisum Sativum L ◽  
Diversity Assessment ◽  
Potential Use ◽  
Simple Sequence

The present study was conducted on genetic diversity analyses among 24 pea genotypes during 2017–2018 to assess the molecular diversity of pea genotypes using SSR markers. Out of 62, eleven markers were found to be polymorphic and the polymorphic information content (PIC) of the simple sequence repeat (SSR) markers ranged from 0.19 to as high as 0.64. Molecular profiling of these genotypes using 11 SSRs distributed throughout the genome generated 32 alleles with a mean of 2.91 alleles per locus. The genetic dissimilarity based on simple matching coefficient for 24 genotypes ranged from 0.00 to 0.91 with an average of 0.52. Cluster analyses grouped 24 genotypes into two major clusters with one outlier and supported by principal coordinate analysis (PCoA) in which genotypes were distributed across four quadrangles. Analysis of molecular variance (AMOVA) showed significant estimated value at degree of 1000 permutations. Percentage of variability was higher among individual (67%) than among populations (11%). Percentage of variability within individual was also higher (22%) than among populations (11%). Pop1 (I=0.707, He=0.446, and uHe=0.466) shows higher diversity than pop2 (I=0.630, He=0.381 and uHe=0.398). The percentage of polymorphic loci per population (PPL) ranged from 81.82% (pop2) to 90.91% (pop1) with an average of 86.36%. The present study demonstrates the utility of microsatellite markers for estimating molecular diversity as well as genotype identification in pea. This study also suggests a potential use of these markers in further association studies.

Characterisation of cereal cyst nematodes in Egypt based on morphometrics, RFLP and rDNA-ITS sequence analyses

Nematology ◽  
2015 ◽  
Vol 17 (1) ◽  
pp. 103-115 ◽  
Author(s):  
Mohamed Baklawa ◽  
Björn Niere ◽  
Holger Heuer ◽  
Samia Massoud
Keyword(s):  
Saudi Arabia ◽  
Molecular Diversity ◽  
Its Region ◽  
Restriction Enzymes ◽  
Its Sequence ◽  
Cyst Nematodes ◽  
Sequence Analyses ◽  
Second Stage ◽  
Rdna Its ◽  
Production Areas

Morphological and molecular diversity among populations of cereal cyst nematodes (CCN) from wheat production areas in Ismailia province, Egypt, was investigated using light microscopy, ITS-RFLP and sequencing of the rDNA-ITS. CCN were found in five out of seven regions in Ismailia, the highest incidence being found in El Shark (West Sinai). The Egyptian populations were identified as H. avenae according to morphometrics of cyst vulval cone and second-stage juveniles. No differences in ITS-RFLP patterns generated by 17 restriction enzymes were detected among the Egyptian populations although the Egyptian populations could be distinguished from German populations of H. avenae and H. filipjevi. The analyses of ITS region sequences confirmed the species identification of the Egyptian populations as they clustered with H. avenae populations from Iran, Saudi Arabia, India, Israel and China.

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