scholarly journals Molecular diversity assessment of AVRDC–The World Vegetable Center elite-parental mungbeans

2009 ◽  
Vol 59 (2) ◽  
pp. 149-157 ◽  
Author(s):  
Prakit Somta ◽  
Warunee Sommanas ◽  
Peerasak Srinives
Keyword(s):  
Molecular Diversity ◽  
Diversity Assessment ◽  
The World

scholarly journals SSR DNA marker aided genetic diversity assessment of selected willow clones

Genetika ◽  
2013 ◽  
Vol 45 (2) ◽  
pp. 527-536 ◽  
Author(s):  
N.B. Singh ◽  
S. Joshi ◽  
P. Choudhary ◽  
J.P. Sharma
Keyword(s):  
Genetic Diversity ◽  
Molecular Diversity ◽  
Dna Marker ◽  
Similarity Coefficient ◽  
Gene Pools ◽  
Morphometric Traits ◽  
Genetic Base ◽  
Diversity Assessment ◽  
Ssr Primers ◽  
Clustering Pattern

Around 100 clones of tree willows were subjected for nursery screening twice on morphometric traits. Genetic diversity was assessed in twenty-five genetically superior willow clones hailing from six countries using 16 SSR primers. Fourteen primers amplified the DNA but only ten showed polymorphism. Total 34 bands were scored, out of that 27 were found to be polymorphic and 7 were monomorphic. Three primers showed 100% polymorphism whereas 79.4% polymorphism was recorded in total. The dendrogram obtained from SSR markers revealed that clone SE-69-002 (S. matsudana) and NZ-1040 (S. matsudana X S. alba) as most similar clones (Jaccards coefficient of 0.97), and clone PN-721(S. matsudana X S. alba) and PN-731 (S. nigra), as most divergent clones (Jaccards coefficient of 0.63). All the genotypes were grouped into 4 distinct clusters. On the basis of similarity coefficient analysis the first cluster comprised of 11 genotypes, the second cluster have 8 genotypes where as third one has only one genotype and fourth cluster retained five genotypes. The clustering pattern further indicated that the geographic distribution may not be the reflection of genetic diversity in willow clones. Genotypes with high molecular diversity could be used in breeding programme in order to obtain heterotic hybrids and development of gene pools with broad genetic base. The genotype specific bands developed by the SSR primers could also be used for identification of cultivar.

scholarly journals Microbial Transformations of Organically Fermented Foods

Metabolites ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 165 ◽  
Author(s):  
Ruma Raghuvanshi ◽  
Allyssa G. Grayson ◽  
Isabella Schena ◽  
Onyebuchi Amanze ◽  
Kezia Suwintono ◽  
...  
Keyword(s):  
Natural Products ◽  
Fermentation Process ◽  
Molecular Diversity ◽  
Food Type ◽  
Fermented Foods ◽  
Microbial Fermentation ◽  
Soil Community ◽  
Specialized Metabolites ◽  
The World ◽  
Salt Brine

Fermenting food is an ancient form of preservation ingrained many in human societies around the world. Westernized diets have moved away from such practices, but even in these cultures, fermented foods are seeing a resurgent interested due to their believed health benefits. Here, we analyze the microbiome and metabolome of organically fermented vegetables, using a salt brine, which is a common ‘at-home’ method of food fermentation. We found that the natural microbial fermentation had a strong effect on the food metabolites, where all four foods (beet, carrot, peppers and radishes) changed through time, with a peak in molecular diversity after 2–3 days and a decrease in diversity during the final stages of the 4-day process. The microbiome of all foods showed a stark transition from one that resembled a soil community to one dominated by Enterobacteriaceae, such as Erwinia spp., within a single day of fermentation and increasing amounts of Lactobacillales through the fermentation process. With particular attention to plant natural products, we observed significant transformations of polyphenols, triterpenoids and anthocyanins, but the degree of this metabolism depended on the food type. Beets, radishes and peppers saw an increase in the abundance of these compounds as the fermentation proceeded, but carrots saw a decrease through time. This study showed that organically fermenting vegetables markedly changed their chemistry and microbiology but resulted in high abundance of Enterobacteriaceae which are not normally considered as probiotics. The release of beneficial plant specialized metabolites was observed, but this depended on the fermented vegetable.

scholarly journals Genetic Diversity Analysis of Sugarcane Parents in Chinese Breeding Programmes Using gSSR Markers

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Qian You ◽  
Liping Xu ◽  
Yifeng Zheng ◽  
Youxiong Que
Keyword(s):  
Genetic Diversity ◽  
Molecular Diversity ◽  
Genetic Relationships ◽  
Breeding Programs ◽  
Sugarcane Varieties ◽  
Breeding Station ◽  
The World ◽  
Breeding Programmes ◽  
Genetic Structures ◽  
Simple Sequence

Sugarcane is the most important sugar and bioenergy crop in the world. The selection and combination of parents for crossing rely on an understanding of their genetic structures and molecular diversity. In the present study, 115 sugarcane genotypes used for parental crossing were genotyped based on five genomic simple sequence repeat marker (gSSR) loci and 88 polymorphic alleles of loci (100%) as detected by capillary electrophoresis. The values of genetic diversity parameters across the populations indicate that the genetic variation intrapopulation (90.5%) was much larger than that of interpopulation (9.5%). Cluster analysis revealed that there were three groups termed as groups I, II, and III within the 115 genotypes. The genotypes released by each breeding programme showed closer genetic relationships, except the YC series released by Hainan sugarcane breeding station. Using principle component analysis (PCA), the first and second principal components accounted for a cumulative 76% of the total variances, in which 43% were for common parents and 33% were for new parents, respectively. The knowledge obtained in this study should be useful to future breeding programs for increasing genetic diversity of sugarcane varieties and cultivars to meet the demand of sugarcane cultivation for sugar and bioenergy use.

scholarly journals Phenological, Nutritional and Molecular Diversity Assessment among 35 Introduced Lentil (Lens culinaris Medik.) Genotypes Grown in Saudi Arabia

2013 ◽  
Vol 15 (1) ◽  
pp. 277-295 ◽  
Author(s):  
Salem Alghamdi ◽  
Altaf Khan ◽  
Megahed Ammar ◽  
Ehab El-Harty ◽  
Hussein Migdadi ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Lens Culinaris ◽  
Molecular Diversity ◽  
Diversity Assessment

scholarly journals Molecular Diversity Assessment of PGPR Using Enterobacterial Repetitive Intergeneric Consensus (ERIC) PCR

2018 ◽  
Vol 7 ◽  
pp. 64-70
Author(s):  
Umesh Prasad Shrivastava
Keyword(s):  
Plant Growth ◽  
Molecular Diversity ◽  
Protein Profile ◽  
Plant Growth Promoting Rhizobacteria ◽  
Rice Fields ◽  
Border Region ◽  
Diversity Assessment ◽  
Plant Growth Promoting ◽  
Growth Promoting

In the present investigation, an attempt has been made to study the diversity of Plant Growth Promoting Rhizobacteria isolated from various rice fields of Indo-Nepal border region. It is reported here that diversity in PGPR of East Champaran, West Champaran and Varanasi district of India and Bara and Parsa district of Nepal showed 76 to 97% similarity among them based on ERIC PCR. Serratia, Agrobacterium, Klebsiella, Pseudomonas etc are predominantly present in the rhizosphere of rice fields of these areas. Protein profile showed that all the isolates were 49-79% similar among each other. The three members consisting species of Klebsiella showed 72% similarity to each other and two species of Agrobacterium showed 80% similarity to each other.

MOLECULAR DIVERSITY ASSESSMENT OF CUMIN ECOTYPES USING AFLP MARKERS

2014 ◽  
pp. 263-270
Author(s):  
A. Bahraminejad ◽  
G. Mohammadinejad ◽  
M. Abdul Kadir
Keyword(s):  
Molecular Diversity ◽  
Aflp Markers ◽  
Diversity Assessment

scholarly journals Genetic Diversity Assessment in Pea (Pisum sativum L.) using Microsatellite Markers

2021 ◽  
Vol 12 (5) ◽  
pp. 402-408
Author(s):  
Hanuman Ram ◽  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Molecular Diversity ◽  
Polymorphic Information Content ◽  
Association Studies ◽  
Pisum Sativum L ◽  
Diversity Assessment ◽  
Potential Use ◽  
Simple Sequence

The present study was conducted on genetic diversity analyses among 24 pea genotypes during 2017–2018 to assess the molecular diversity of pea genotypes using SSR markers. Out of 62, eleven markers were found to be polymorphic and the polymorphic information content (PIC) of the simple sequence repeat (SSR) markers ranged from 0.19 to as high as 0.64. Molecular profiling of these genotypes using 11 SSRs distributed throughout the genome generated 32 alleles with a mean of 2.91 alleles per locus. The genetic dissimilarity based on simple matching coefficient for 24 genotypes ranged from 0.00 to 0.91 with an average of 0.52. Cluster analyses grouped 24 genotypes into two major clusters with one outlier and supported by principal coordinate analysis (PCoA) in which genotypes were distributed across four quadrangles. Analysis of molecular variance (AMOVA) showed significant estimated value at degree of 1000 permutations. Percentage of variability was higher among individual (67%) than among populations (11%). Percentage of variability within individual was also higher (22%) than among populations (11%). Pop1 (I=0.707, He=0.446, and uHe=0.466) shows higher diversity than pop2 (I=0.630, He=0.381 and uHe=0.398). The percentage of polymorphic loci per population (PPL) ranged from 81.82% (pop2) to 90.91% (pop1) with an average of 86.36%. The present study demonstrates the utility of microsatellite markers for estimating molecular diversity as well as genotype identification in pea. This study also suggests a potential use of these markers in further association studies.

scholarly journals Identification of Two New Isolates of Chilli veinal mottle virus From Different Regions in China: Molecular Diversity, Phylogenetic and Recombination Analysis

2020 ◽  
Vol 11 ◽  
Author(s):  
Shaofei Rao ◽  
Xuwei Chen ◽  
Shiyou Qiu ◽  
Jiejun Peng ◽  
Hongying Zheng ◽  
...  
Keyword(s):  
Molecular Diversity ◽  
Important Determinant ◽  
Full Length ◽  
Mottle Virus ◽  
Recombination Analysis ◽  
Yield Losses ◽  
The World ◽  
Evolutionary Event ◽  
First Time ◽  
Chilli Veinal Mottle Virus

Chilli veinal mottle virus (ChiVMV) is an important plant pathogen with a wide host range, causing serious yield losses in pepper production all over the world. Recombination is a major evolutionary event for single-stranded RNA viruses, which helps isolates adapt to new environmental conditions and hosts. Recombination events have been identified in multiple potyviruses, but so far, there have been no reports of recombination events among the ChiVMV population. We here detected ChiVMV in pepper samples collected from Guangxi and Yunnan provinces for the first time and amplified the nearly full-length sequences. Phylogenetic and recombination analysis were performed using the new sequences and the 14 full-length and 23 capsid protein (CP) sequences available in GenBank. Isolates tend to cluster on a geographical basis, indicating that geographic-driven evolution may be an important determinant of ChiVMV genetic differences. A total of 10 recombination events were detected among the ChiVMV sequences using RDP4 with a strict algorithm, and both the Guangxi and Yunnan isolates were identified as recombinants. Recombination appears to be a significant factor affecting the diversity of ChiVMV isolates.

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