scholarly journals Isolation, Screening, and Characterization of Antibiotic-Degrading Bacteria for Penicillin V Potassium (PVK) from Soil on a Pig Farm

2019 ◽  
Vol 16 (12) ◽  
pp. 2166 ◽  
Author(s):  
Xuanjiang Yang ◽  
Miao Li ◽  
Panpan Guo ◽  
Hualong Li ◽  
Zelin Hu ◽  
...  
Keyword(s):  
Contaminated Soils ◽  
Antibiotic Sensitivity ◽  
Animal Husbandry ◽  
Farm Animals ◽  
Bacterial Strains ◽  
Penicillin V ◽  
Degradation Rates ◽  
Antibiotic Susceptibility Test ◽  
Degrading Bacteria ◽  
Pig Farm

(1) Background: Antibiotics are frequently used on farm animals, making animal husbandry a relatively large source of antibiotic pollution of the environment. The present study aims to isolate and acclimatize antibiotic-degrading bacterial strains for penicillin V potassium (PVK) from the contaminated soil of a pig farm. (2) Methods: Bacterial strains were isolated and acclimatized by continuous enrichment of cultures with PVK as the sole carbon source. The antibiotic susceptibility test, thiol mercury salt ultraviolet spectrophotometry (TMSUS), morphological observations, and 16S rDNA sequence analysis were used to identify and characterize the isolated strains. (3) Results: Four bacterial isolates (denoted as LM-1, LM-2, LM-3, LM-4) were obtained, and two of them (LM-1, LM-2) with the highest degradation rates were identified to belong to the same genera as Bacillus. These two isolates were found to be resistant to PVK antibiotic in an antibiotic sensitivity test. The TMSUS indicated that the strains LM-1 and LM-2 had good performance in PVK degradation (68% for LM-1, 66% for LM-2 in 48 h) when the initial PVK concentration was about 100 μg/mL. (4) Conclusions: Two bacterial strains isolated from the soil on a pig farm are effective in degrading PVK and can be potentially used for bioremediation of PVK antibiotic-contaminated soils.

Characteristics of phenanthrene-degrading bacteria isolated from soils contaminated with polycyclic aromatic hydrocarbons

1998 ◽  
Vol 44 (8) ◽  
pp. 743-752 ◽  
Author(s):  
Michael D Aitken ◽  
William T Stringfellow ◽  
Robert D Nagel ◽  
Chikoma Kazunga ◽  
Shu-Hwa Chen
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Contaminated Soils ◽  
Acid Methyl Ester ◽  
Aqueous Solubility ◽  
Bacterial Strains ◽  
Phenanthrene Degradation ◽  
Acid Methyl ◽  
Degrading Bacteria ◽  
Polycyclic Aromatic

Ten bacterial strains were isolated from seven contaminated soils by enrichment with phenanthrene as the sole carbon source. These isolates and another phenanthrene-degrading strain were examined for various characteristics related to phenanthrene degradation and their ability to metabolize 12 other polycyclic aromatic hydrocarbons (PAH), ranging in size from two to five rings, after growth in the presence of phenanthrene. Fatty acid methyl ester analysis indicated that at least five genera (Agrobacterium, Bacillus, Burkholderia, Pseudomonas, and Sphingomonas) and at least three species of Pseudomonas were represented in this collection. All of the strains oxidized phenanthrene according to Michaelis-Menten kinetics, with half-saturation coefficients well below the aqueous solubility of phenanthrene in all cases. All but one of the strains oxidized 1-hydroxy-2-naphthoate following growth on phenanthrene, and all oxidized at least one downstream intermediate from either or both of the known phenanthrene degradation pathways. All of the isolates could metabolize (oxidize, mineralize, or remove from solution) a broad range of PAH, although the exact range and extent of metabolism for a given substrate were unique to the particular isolate. Benz[a]anthracene, chrysene, and benzo[a]pyrene were each mineralized by eight of the strains, while pyrene was not mineralized by any. Pyrene was, however, removed from solution by all of the isolates, and the presence of at least one significant metabolite from pyrene was observed by radiochromatography for the five strains in which such metabolites were sought. Our results support earlier indications that the mineralization of pyrene by bacteria may require unique metabolic capabilities that do not appear to overlap with the determinants for mineralization of phenanthrene or other high molecular weight PAH.Key words: kinetics, polycyclic aromatic hydrocarbons, phenanthrene, mineralization, benzo[a]pyrene.

scholarly journals Erratum: Yang, X., et al. Isolation, Screening, and Characterization of Antibiotic-Degrading Bacteria for Penicillin V Potassium (PVK) from Soil on a Pig Farm. Int. J. Environ. Res. Public Health 2019, 16, 2166

2021 ◽  
Vol 18 (2) ◽  
pp. 678
Author(s):  
Xuanjiang Yang ◽  
Miao Li ◽  
Panpan Guo ◽  
Hualong Li ◽  
Zelin Hu ◽  
...  
Keyword(s):  
Public Health ◽  
Penicillin V ◽  
Degrading Bacteria ◽  
Pig Farm

The second affiliation of the paper should have been included in our original article [...]

Misuse of Antibiotics and the Dissemination of Antibiotic Resistant Bacteria in the Community

2020 ◽  
Vol 4 (1) ◽  
pp. 91-106
Author(s):  
Cassandra Sturgeon Delia
Keyword(s):  
Clinical Setting ◽  
Animal Husbandry ◽  
Medical Professional ◽  
Farm Animals ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Health Related ◽  
Behavioural Factors

A crucial issue that threatens humanity worldwide, is the misuse of antibiotics (Marquard & Li, 2018). However, the terms misuse and overuse of antibiotics are widely misunderstood, as many assume that antibiotics are only acquired directly through a prescription by a medical professional. The reality of the situation is much more complex, and many do not realise the indirect intake through ingestion with food (Philips et al., 2003) and recreational actions (Schwartz et al., 2003). Moreover, such information is kept out of the limelight, keeping the community unaware of this pressing issue. Antibiotic resistance is escalating globally as social behaviour is leading to selective pressure creating resistant strains of bacteria through excessive exploitation of antibiotics (Okeke & Edelman, 1999). This article aims to address the mechanisms of antibiotic-resistant bacteria and the link to healthy individuals’ gut flora, creating asymptomatic carriers within the community. Since many students at MCAST are undertaking courses that may aid in the transportation of antibiotic-resistant bacteria, such as animal husbandry, they need to be aware of bacterial strains found in farm animals, which pose a potential risk to humans via the food chain. Students undergoing courses that lead to health-related work, seeking future employment in a clinical setting, also need to be aware of the threat antibiotic-resistant pathogens pose to humans advancing from a clinical setting to the community. Persons working within such industries need to understand both how pathogens gain resistance, and how they spread, to apprehend methods of avoiding transmission. This study endeavours to increase local awareness within the community, and avoid this socioeconomic threat, by addressing behavioural factors.

scholarly journals Antibiotic Sensitivity Pattern of Diverse Soil and Air Flora of Federal University of Technology campus, Akure, Nigeria

2021 ◽  
pp. 18-39
Author(s):  
Kayode Anthony Onifade ◽  
Naomi Titilayo Akinsola
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Cereus ◽  
Health Centre ◽  
Antibiotic Sensitivity ◽  
Bacterial Strains ◽  
Potential Threat ◽  
Agar Disc ◽  
Sensitivity Pattern ◽  
Antibiotic Susceptibility Test ◽  
Ondo State

Aim: This study was conducted to comparatively investigate the soil and air microflora of selected locations within the Federal University of Akure (FUTA), Ondo State, Nigeria. Study design: Experimental design Place and Duration of Study: The study was conducted at The Federal University Technology, Akure (FUTA), Ondo State, Nigeria comprising; School of Sciences, School of Agricultural and Agricultural Technology, School of Engineering and Engineering Technology, School of Health and Health Technology, Microbiology department, FUTA Health Centre, postgraduate hostel, female hostels (main Jibowu and Jibowu annex 1) and the male hostels (Akindeko and Abiola hall of residence) between June and July, 2019. Methodology: Isolation of bacteria and fungi from soil samples and air was conducted using specified techniques. Antibiotic susceptibility test was conducted via agar disc diffusion technique. Plasmid analysis and curing was conducted via standard protocols. Results: Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Proteus mirabilis, Bacillus cereus, B. subtilis, Staphylococcus aureus, S. saprophyticus were isolated as soil and air microflora while Candida albicans, Aspergillus fumigatus, with Fusarium oxysporium were isolated from soil as A. flavus, A. niger, Penicillium chrysogenum was implicated in air. Bacillus cereus and B. subtilis showed utmost resistance to ceftazidime, cefuroxime, cloxacillin, augmentin, ceftriaxone, whereas Enterobacter aerogenes and Proteus vulgaris were susceptible to gentamycin at 17.66±1.52 mm and 16.00±2.00 mm respectively. Staphylococcus aureus was sensitive to gentamycin and ofloxacin at 17.30±0.57 mm and 18.66±0.57 mm. The multidrug resistant (MDR) bacterial strains were positive for plasmid DNA with 10 kilobase pairs, but were sensitive to all the antibiotics after curing indicating plasmid-borne resistance. Conclusion: Findings from this study inferred possible health consequences of MDR soil and air microflora as potential threat to human health which necessitates proper sanitary practices across different sampling areas adopted for this study to reduce potential incidence of bacterial and mycotic infections.

scholarly journals Resistance profile and virulence characterization of Escherichia coli isolated from diarrheic neonatal farm animals

2021 ◽  
Vol 42 (2) ◽  
pp. 735-746
Author(s):  
Raffaella Menegheti Mainardi ◽  
◽  
Arthur Roberto da Costa ◽  
Roberta Torres Chideroli ◽  
Leonardo Mantovani Favero ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Clinical Signs ◽  
Animal Husbandry ◽  
Multidrug Resistant ◽  
Farm Animals ◽  
Bacterial Strains ◽  
Antimicrobial Drugs ◽  
Mortality And Morbidity ◽  
E Coli

Neonatal diarrhea is the main cause of early mortality and morbidity in farm animals and the source of huge, direct and indirect, economic husbandry losses. Escherichia coli, a common harmless commensal bacterium, can turn into a main diarrheal pathogen through antibiotic resistance and the expression of genetically acquired virulence factors. In this study, fecal samples obtained from eight farms of animals with clinical signs characteristic of diarrhea were subjected to culture and bacterial isolation. Colonies suggestive of E. coli were identified through morphological and biochemical characteristics. Susceptibility tests to the main veterinary antibacterial agents were conducted using agar disk diffusion followed by phenotypical detection of extended-spectrum ?-lactamase (ESBL). A total of 301 colonies were characterized as E. coli and, out of the 192 that were tested, 134 showed resistance to three or more classes of antimicrobial drugs and were classified as multidrug resistant (MDR), and 14 were ESBL positive. Bacterial DNA was extracted for multiplex PCR (mPCR) using primers to detect ten different genes of diarrheagenic E. coli (DEC). Thirty-six bacterial strains were positive in the mPCR assay, 28 of which were classified as enterotoxigenic E. coli (ETEC) and eight as enteropathogenic E. coli (EPEC). The high prevalence of MDR strains and the detection of ESBL denote the presence of resistance genes in animal husbandry; thus, it is important to isolate and characterize those pathogens and test antimicrobial sensitivity in vitro to avoid ineffective treatments and the spread of antimicrobial resistance, which are the major concerns of Public Health and One Health.

scholarly journals Kinetic Study of the Biodegradation of Acephate by Indigenous Soil Bacterial Isolates in the Presence of Humic Acid and Metal Ions

Biomolecules ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 433 ◽  
Author(s):  
Simranjeet Singh ◽  
Vijay Kumar ◽  
Sourav Singla ◽  
Minaxi Sharma ◽  
Dhananjaya P. Singh ◽  
...  
Keyword(s):  
Humic Acid ◽  
Metal Ions ◽  
Contaminated Soils ◽  
Mass Spectrometry Analysis ◽  
Bacterial Strains ◽  
Spectrometry Analysis ◽  
First Order ◽  
First Order Kinetics ◽  
Degradation Rates ◽  
Uv Visible

Many bacteria have the potential to use specific pesticides as a source of carbon, phosphorous, nitrogen and sulphur. Acephate degradation by microbes is considered to be a safe and effective method. The overall aim of the present study was to identify acephate biodegrading microorganisms and to investigate the degradation rates of acephate under the stress of humic acid and most common metal ions Fe(III) and copper Cu(II). Pseudomonas azotoformanss strain ACP1, Pseudomonas aeruginosa strain ACP2, and Pseudomonas putida ACP3 were isolated from acephate contaminated soils. Acephate of concentration 100 ppm was incubated with separate strain inoculums and periodic samples were drawn for UV—visible, FTIR (Fourier-transform infrared spectroscopy) and MS (Mass Spectrometry) analysis. Methamidophos, S-methyl O-hydrogen phosphorothioamidate, phosphenothioic S-acid, and phosphenamide were the major metabolites formed during the degradation of acephate. The rate of degradation was applied using pseudo-first-order kinetics to calculate the half-life (t1/2) values, which were 14.33–16.72 d−1 (strain(s) + acephate), 18.81–21.50 d−1 (strain(s) + acephate + Cu(II)), 20.06 –23.15 d−1 (strain(s) + acephate + Fe(II)), and 15.05–17.70 d−1 (strains + acephate + HA). The biodegradation efficiency of the three bacterial strains can be ordered as P. aeruginosa > P. putida > P. azotoformans. The present study illustrated the decomposition mechanism of acephate under different conditions, and the same may be applied to the removal of other xenobiotic compounds.

scholarly journals Bacteriological profile and antibiotic resistance in cases of chronic otitis media and its clinical implications

2018 ◽  
Vol 4 (4) ◽  
pp. 918
Author(s):  
Ajay Mallick ◽  
Harikesh Sharma ◽  
Awadhesh K. Mishra ◽  
Nidhi V. Maggon ◽  
Ashwani Sethi
Keyword(s):  
Antibiotic Resistance ◽  
Otitis Media ◽  
Tertiary Care ◽  
Chronic Otitis Media ◽  
Antibiotic Sensitivity ◽  
Bacterial Strains ◽  
Tertiary Care Centre ◽  
Bacteriological Diagnosis ◽  
Sensitivity Pattern ◽  
Antibiotic Susceptibility Test

<p class="abstract"><strong>Background:</strong> Chronic otitis media (COM) is the commonest otologic conditions worldwide with great risk of irreversible complications. Early bacteriological diagnosis is essential for appropriate effective therapy to reduce the disease burden. However in our clinical practice, it was noticed that the morbidity of the disease was mostly non responsive to standard antibiotic medical care. The poor response to routine treatment gave rise to suspicion of antibiotic resistance. This prompted us to undertake this study with the objective to isolate and identify the causative organisms in COM in our clientele and study their antimicrobial susceptibility pattern for a better analysis and institution of accurate therapy.</p><p class="abstract"><strong>Methods:</strong> A prospective cross sectional analysis was performed using culture and antibiotic susceptibility test results of 500 patients who visited the ENT OPD at a tertiary care centre between July 2016 to June 2017.  </p><p class="abstract"><strong>Results:</strong> A positive culture was obtained in 466/500 (93.2%) of the samples. No growth was seen in 34 patients (6.8%). 454 samples showed single bacterium while 12 samples had mixed growth. <em>Pseudomonas aeruginosa</em> was the <em>predominant organism</em> (54%) followed by <em>Staphylococcus aureus</em>. On antibiotic sensitivity, high resistance patterns to most common first line antibiotics used in COM was observed.</p><p class="abstract"><strong>Conclusions:</strong> Irrational use of antibiotics for management of COM has led to the emergence of multidrug-resistant bacterial strains. Evaluation of micro bacterial growth and their antibiotic sensitivity pattern in local area helps in prescribing empirical antibiotics for successful treatment of COM.</p><p class="abstract"> </p><p class="keywords"> </p>

A RETROSPECTIVE STUDY ON ANTIBIOTIC USAGE IN A TERTIARY CARE HOSPITAL

2020 ◽  
Vol 4 (6) ◽  
Author(s):  
Anna Joy ◽  
Aparna Anand ◽  
Arathy R Nath ◽  
Meera S Nair ◽  
Dr. K. G. Prasanth
Keyword(s):  
Retrospective Study ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
Actual Process ◽  
Care Hospital ◽  
Rational Use ◽  
Antibiotic Susceptibility Test ◽  
Antibiotic Sensitivity Test ◽  
Rational Use Of Antibiotics

Antibiotics are one of the most commonly prescribed drugs today. Rational use of antibiotics is therefore extremely important as their injudicious use can adversely affect the patient. Drug Utilization Evaluation (DUE) is a system of ongoing systematic criteria based evaluation of drug that will help to ensure that medicines are used appropriately. It is drug/disease specific and can be structured so that it will assess the actual process of prescribing, dispensing, or administration of drug. The retrospective study was conducted At Pk Das Institute of Medical Sciences, Palakkad, Kerala for a duration of 6 months (February 2017 - January 2018). A source of data includes Patient case sheets &medication charts, nursing charts, culture & sensitivity reports. The inclusion criterion includes Patients aged between 18- 80 year, prescribed with oral and parenteral antibiotics. . On analyzing the gender, male gender (n= 111, 55.5%) were higher in numbers as compared to female counterparts (n=89, 44.5%). In our study the majority of the patients prescribed with antibiotics were with the clinical assessment of COPD (n=39, 19.5%), UTI (n=37, 18.5%) and LRTI (n=28, 14%), Bronchial asthma (n=19, 9.5%) respectively. On analyzing the data based on antibiotic sensitivity test, antibiotic test were performed and followed in (n=64,32%)prescriptions and in (n=47,23.5%)prescriptions were test is not followed respectively. In (n=89,44.5%) prescriptions, antibiotic sensitivity test is not performed. On analysis of antibiotics prescribed, the most commonly prescribed antibiotics were cephalosporins, of these ceftriaxone was highly prescribed of all (n=95). The high percentage of antibiotic prescriptions may indicate a high probability of irrational use. This study also point out irrational use of antibiotics are more leading to resistance, misuse and serious problems. So certain strategies should be put forward to strengthen rational use of antibiotics. Keywords: Antibiotics, Antibiotic Susceptibility Test, Irrational use, Resistance

Biodegradation of organophosphorus Pollutants by Soil Bacteria: Biochemical Aspects and Unsolved Problems

2020 ◽  
Vol 36 (4) ◽  
pp. 126-135
Author(s):  
T.V. Shushkova ◽  
D.O. Epiktetov ◽  
S.V. Tarlachkov ◽  
I.T. Ermakova ◽  
A.A. Leontievskii
Keyword(s):  
Genome Sequencing ◽  
Soil Bacteria ◽  
Activity Regulation ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Russian Science ◽  
Degrading Bacteria ◽  
Phosphorus Source ◽  
Glyphosate Herbicide ◽  
Enzymatic Pathways

The degradation of persistent organophosphorus pollutants have been studied in 6 soil bacterial isolates and in 3 bacterial strains adapted for utilization of glyphosate herbicide (GP) under laboratory conditions. Significant differences in the uptake of organophosphonates were found in taxonomically close strains possessing similar enzymatic pathways of catabolism of these compounds, which indicates the existence of unknown mechanisms of activity regulation of these enzymes. The effect of adaptation for GP utilization as a sole phosphorus source on assimilation rates of several other phosphonates was observed in studied bacteria. The newly found efficient stains provided up to 56% of GP decomposition after application to the soil in the laboratory. The unresolved problems of microbial GP metabolism and the trends for further research on the creation of reliable biologicals capable of decomposing organophosphonates in the environment are discussed. organophosphonates, glyphosate, biodegradation, bioremediation, C-P lyase, phosphonatase, degrading bacteria Investigation of phosphonatase and genome sequencing were supported by Russian Science Foundation Grant no. 18-074-00021.

Biological granulated activated carbon fluidized bed reactor for atrazine remediation

2004 ◽  
Vol 49 (11-12) ◽  
pp. 215-222 ◽  
Author(s):  
M. Herzberg ◽  
C.G. Dosoretz ◽  
S. Tarre ◽  
M. Beliavski ◽  
M. Green
Keyword(s):  
Fluidized Bed ◽  
Fluidized Bed Reactor ◽  
Batch Adsorption ◽  
Pseudomonas Sp ◽  
Partial Penetration ◽  
Biofilm Carrier ◽  
Degradation Rates ◽  
Degrading Bacteria ◽  
Atrazine Degradation ◽  
Granulated Activated Carbon

To show that an adsorbing biofilm carrier (GAC) can be advantageous for atrazine bioremediation over a non-adsorbing carrier, fluidized bed (FB) reactors were operated under atrazine limiting concentrations using Pseudomonas sp. strain ADP as the atrazine degrading bacteria. The following interrelated subjects were investigated: 1) atrazine adsorption to GAC under conditions of atrazine partial penetration in the biofilm, 2) differences in atrazine degradation rates and 3) stability of atrazine biodegradation under non-sterile anoxic conditions in the GAC reactor versus a reactor with a non-adsorbing biofilm carrier. Results from batch adsorption tests together with modeling best described the biofilm as patchy in nature with covered and non-biofilm covered areas. Under conditions of atrazine partial penetration in the biofilm, atrazine adsorption occurs in the non-covered areas and is consequently desorbed at the base of the biofilm substantially increasing the active biofilm surface area. The double flux of atrazine to the biofilm in the GAC reactor results in lower effluent atrazine concentrations as compared to a FB reactor with a non-adsorbing carrier. Moreover, under non-sterile denitrification conditions, atrazine degradation stability was found to be much higher (several months) using GAC as a biofilm carrier while non-adsorbing carrier reactors showed sharp deterioration within 30 days due to contamination of non-atrazine degrading bacteria.

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