scholarly journals Analysis of Phenacylester Derivatives of Fatty Acids from Human Skin Surface Sebum by Reversed-Phase HPLC: Chromatographic Mobility as a Function of Physico-Chemical Properties

2007 ◽  
Vol 8 (11) ◽  
pp. 1111-1124 ◽  
Author(s):  
Juliana Bodoprost ◽  
Helmut Rosemeyer
Keyword(s):  
Fatty Acids ◽  
Human Skin ◽  
Chemical Properties ◽  
Skin Surface ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Chromatographic Mobility ◽  
Physico Chemical ◽  
Derivatives Of

scholarly journals Host/Malassezia Interaction: A Quantitative, Non-Invasive Method Profiling Oxylipin Production Associates Human Skin Eicosanoids with Malassezia

Metabolites ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 700
Author(s):  
Yohannes Abere Ambaw ◽  
Martin P. Pagac ◽  
Antony S. Irudayaswamy ◽  
Manfred Raida ◽  
Anne K. Bendt ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Immune System ◽  
Polyunsaturated Fatty Acids ◽  
Human Skin ◽  
Skin Surface ◽  
Lipid Mediators ◽  
Dependent Manner ◽  
Human Immune System ◽  
Plant Host

Malassezia are common components of human skin, and as the dominant human skin eukaryotic microbe, they take part in complex microbe–host interactions. Other phylogenetically related fungi (including within Ustilagomycotina) communicate with their plant host through bioactive oxygenated polyunsaturated fatty acids, generally known as oxylipins, by regulating the plant immune system to increase their virulence. Oxylipins are similar in structure and function to human eicosanoids, which modulate the human immune system. This study reports the development of a highly sensitive mass-spectrometry-based method to capture and quantify bioactive oxygenated polyunsaturated fatty acids from the human skin surface and in vitro Malassezia cultures. It confirms that Malassezia are capable of synthesizing eicosanoid-like lipid mediators in vitro in a species dependent manner, many of which are found on human skin. This method enables sensitive identification and quantification of bioactive lipid mediators from human skin that may be derived from metabolic pathways shared between skin and its microbial residents. This enables better cross-disciplinary and detailed studies to dissect the interaction between Malassezia and human skin, and to identify potential intervention points to promote or abrogate inflammation and to improve human skin health.

scholarly journals Chemical composition of germinating Tunisian almond (Prunus Amygdalus Mill.) seedlings oil

2021 ◽  
Vol 48 (2) ◽  
Author(s):  
Refka Dhouibi ◽  
◽  
Hanen Oueslati ◽  
Senda Bahri ◽  
Khaled Jabou ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Lipolytic Activity ◽  
Chemical Properties ◽  
Dry Mass ◽  
Chemical Components ◽  
Prunus Amygdalus ◽  
Almond Oil ◽  
Physico Chemical ◽  
Total Fatty Acids

Almonds (Prunus amygdalus) are a rich source of many essential nutrients. However, there is a lack of enough information on almond varieties' biochemical composition, especially at the germination stage. Therefore, this study was conducted to determine the chemical components of the germinating Tunisian almonds. The study included determining the content of oils, proteins, fatty acids, and triglycerides during germination. Results indicated that the oleaginous seeds are rich in oil (55 to 65% of the dry mass) and crude protein (21.825 mg/mL). The dominant polyunsaturated fatty acids are oleic and linoleic acids which represent 64.53% and 24.38%, respectively, while palmitic acid is the most dominant saturated fatty acid with 7.65% of the total fatty acids. Also, the primary molecular types of triglycerides detected by L.C. analysis are triolein (32.3%) and dioleolinolein (24.0%), followed by palmitodiolein (12.5%) and oleodilinolein (12.6%.). The physico-chemical properties study revealed that almond oil remains stable, thus preserving its quality and nutritional value, even during transition from dormancy to germination. On the other hand, we also detected the presence of a lipolytic activity which is maximum on the 3rd day of germination (4.66 mUI). Our results indicate that almond oil plays an important role in human nutrition due to the presence of unsaturated fatty acids, and it is more stable than other oils.

scholarly journals Interaction between SARS-CoV-2 spike glycoprotein and human skin models: a molecular dynamics study

2021 ◽  
Author(s):  
Marc Domingo ◽  
Jordi Faraudo
Keyword(s):  
Stratum Corneum ◽  
Human Skin ◽  
Skin Surface ◽  
Viral Envelope ◽  
Solid Surfaces ◽  
Spike Glycoprotein ◽  
Indirect Transmission ◽  
Physico Chemical ◽  
Human Skin Models ◽  
Structure Orientation

The possibility of contamination of human skin by infectious virions plays an important role in indirect transmission of respiratory viruses but little is known about the fundamental physico-chemical aspects of the virus-skin interactions. In the case of coronaviruses, the interaction with surfaces (including the skin surface) is mediated by their large glycoprotein spikes that protrude from (and cover) the viral envelope. Here, we perform all atomic simulations between the SARS-CoV-2 spike glycoprotein and human skin models. We consider an "oily" skin covered by sebum and a "clean" skin exposing the stratum corneum. The simulations show that the spike tries to maximize the contacts with stratum corneum lipids, particularly ceramides, with substantial hydrogen bonding. In the case of "oily" skin, the spike is able to retain its structure, orientation and hydration over sebum with little interaction with sebum components. Comparison of these results with our previous simulations of the interaction of SARS-CoV-2 spike with hydrophilic and hydrophobic solid surfaces, suggests that the"soft" or "hard" nature of the surface plays an essential role in the interaction of the spike protein with materials.

scholarly journals Photoaffinity labelling of cyanomethaemoglobin with derivatives of tryptophan and 5-bromotryptophan

1995 ◽  
Vol 308 (1) ◽  
pp. 251-260 ◽  
Author(s):  
M Li ◽  
Z Lin ◽  
M E Johnson
Keyword(s):  
Amino Acid ◽  
Binding Sites ◽  
Tryptic Peptide ◽  
Fast Atom Bombardment ◽  
Binding Mode ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Photoaffinity Labelling ◽  
Derivatives Of ◽  
Fast Atom Bombardment Ms

Tryptophan and 5-bromotryptophan (5-BrTrp) are relatively potent inhibitors of sickle-haemoglobin polymerization. The binding sites of these compounds to normal and sickle haemoglobin (HBA and HBS) have been suggested, but not firmly established, through the use of spin-labelled derivatives and/or computer modeling. In the present study we approached the problem by utilizing the technique of photoaffinity labelling. The cyanomet forms of HBA and HBS were subjected to photoaffinity labelling with N alpha-(4-azidotetrafluorobenzoyl)tryptophan and N alpha-(1-ethyl-2-diazomalonyl)-5-bromotryptophan respectively. Both irradiated samples of HBA and HBS were denatured, digested with trypsin, and then separated by reversed-phase HPLC. A labelled tryptic peptide was isolated from the photolabelling of HBS with N alpha-(1-ethyl-2-diazomalonyl)-5-bromotryptophan. The peptide was identified to be Val1(alpha)-Lys7(alpha), with the label attached to Val1(alpha), by virtue of amino acid analysis and sequencing, in conjunction with fast-atom-bombardment MS. The binding mode of N alpha-(1-ethyl-2-diazomalonyl)-5-bromotryptophan is proposed and its relevance to the potency of the 5-BrTrp-based anti-sickling agents is discussed.

Combination of solid-state NMR and 1H NMR relaxometry for the study of intercalated saponite clays with the macrocyclic derivatives of Gd(iii) and Y(iii)

2020 ◽  
Vol 49 (20) ◽  
pp. 6566-6571 ◽  
Author(s):  
Daniela Lalli ◽  
Stefano Marchesi ◽  
Fabio Carniato ◽  
Chiara Bisio ◽  
Lorenzo Tei ◽  
...  
Keyword(s):  
Solid State ◽  
1H Nmr ◽  
Solid State Nmr ◽  
Chemical Properties ◽  
H Nmr ◽  
Nmr Relaxometry ◽  
Physico Chemical ◽  
Derivatives Of ◽  
1H Nmr Relaxometry ◽  
Synthetic Saponite

A combination of solid-state NMR and 1H-NMR relaxometric investigations has been employed to characterize the structure and physico-chemical properties of a novel synthetic saponite intercalated with Gd(iii) and Y(iii) chelates.

The physico-chemical properties of some citrus seeds and seed oils

2016 ◽  
Vol 71 (3-4) ◽  
pp. 79-85 ◽  
Author(s):  
Fahad A.L. Juhaimi ◽  
Bertrand Matthäus ◽  
Mehmet Musa Özcan ◽  
Kashif Ghafoor
Keyword(s):  
Fatty Acids ◽  
Chemical Properties ◽  
Seed Oils ◽  
Mineral Contents ◽  
Bitter Orange ◽  
Physico Chemical ◽  
Citrus Seed ◽  
Citrus Seeds ◽  
Kinnow Mandarin ◽  
Oleic And Linoleic Acids

Abstract The chemical properties, mineral contents, fatty acid and tocopherol contents of seed and seed oils of some citrus genus provided from several locations in Turkey and Saudi Arabia were determined. While Ca contents of seeds were between 5018 mg/kg (Kütdiken lemon) and 7619 mg/kg (kinnow mandarin), K contents of seeds varied between 7007 mg/kg (Orlando orange) and 10334 mg/kg (kinnow mandarin). Glucose and fructose contents of citrus seed samples varied between 3.75 g/kg and 5.75 g/kg, and 4.09 g/kg and 6.03 g/kg. Palmitic, oleic and linoleic acids were established as dominant fatty acids. Palmitic, oleic and linoleic acid contents of citrus seed oils varied between 19.6% (Kütdiken lemon) and 26.2% (pineapple orange), 21.3% (kinnow mandarin) and 31.4% (Kütdiken lemon) and 32.3% (Kütdiken lemon) and 43.7% (kinnow mandarin), respectively. The total amount of tocopherols of Turkish citrus oil varied between 0.5 mg/100 g (Fremont mandarin) and 18.8 mg/100 g (bitter orange).

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