scholarly journals Strawberry FaWRKY25 Transcription Factor Negatively Regulated the Resistance of Strawberry Fruits to Botrytis cinerea

Genes ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 56
Author(s):  
Sizhen Jia ◽  
Yuanhua Wang ◽  
Geng Zhang ◽  
Zhiming Yan ◽  
Qingsheng Cai
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Jasmonic Acid ◽  
Botrytis Cinerea ◽  
Crucial Role ◽  
Defense Mechanism ◽  
Resistance Mechanism ◽  
Expression Level ◽  
Biotic And Abiotic Stress ◽  
Strawberry Resistance

WRKY genes and jasmonic acid (JA) play a crucial role in plants’ responses against biotic and abiotic stress. However, the regulating mechanism of WRKY genes on strawberry fruits’ resistance against Botrytis cinerea is largely unknown, and few studies have been performed on their effect on the JA-mediated defense mechanism against B. cinerea. This study explored the effect of FaWRKY25 on the JA-mediated strawberry resistance against B. cinerea. Results showed that the JA content decreased significantly as the fruits matured, whereas the FaWRKY25 expression rose substantially, which led to heightened susceptibility to B. cinerea and in strawberries. External JA treatment significantly increased the JA content in strawberries and reduced the FaWRKY25 expression, thereby enhancing the fruits’ resistance against B. cinerea. FaWRKY25 overexpression significantly lowered the fruits’ resistance against B. cinerea, whereas FaWRKY25 silencing significantly increased resistance. Moreover, FaWRKY25 overexpression significantly lowered the JA content, whereas FaWRKY25 silencing significantly increased it. FaWRKY25 expression level substantially affects the expression levels of genes related to JA biosynthesis and metabolism, other members of the WRKY family, and defense genes. Accordingly, FaWRKY25 plays a crucial role in regulating strawberries’ resistance against B. cinerea and may negatively regulate their JA-mediated resistance mechanism against B. cinerea.

scholarly journals Proteomic profile of Piper tuberculatum (Piperaceae)

2017 ◽  
Vol 78 (1) ◽  
pp. 117-124 ◽  
Author(s):  
F. Cotinguiba ◽  
S. N. López ◽  
I. G. F. Budzinski ◽  
C. A. Labate ◽  
M. J. Kato ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Abiotic Stress ◽  
Secondary Metabolites ◽  
Defense Mechanism ◽  
Biological Activities ◽  
Proteomic Profile ◽  
Biotic And Abiotic Stress ◽  
Sds Page ◽  
Proteomic Study ◽  
Molecular Information

Abstract Piper tuberculatum (Piperaceae) is a species that accumulates especially amides as secondary metabolites and several biological activities was previously reported. In this article, we report a proteomic study of P. tuberculatum. Bidimensional electrophoresis (2D SDS-PAGE) and mass spectrometry (ESI-Q-TOF) were used in this study. Over a hundred spots and various peptides were identified in this species and the putative functions of these peptides related to defense mechanism as biotic and abiotic stress were assigned. The information presented extend the range of molecular information of P. tuberculatum.

scholarly journals Association of transcription factor WRKY56 gene from Populus simonii × P. nigra with salt tolerance in Arabidopsis thaliana

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7291 ◽  
Author(s):  
Lei Wang ◽  
Wenjing Yao ◽  
Yao Sun ◽  
Jiying Wang ◽  
Tingbo Jiang
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Germination Rate ◽  
Wrky Transcription Factor ◽  
Biotic And Abiotic Stress ◽  
Populus Simonii ◽  
Reading Frame

The WRKY transcription factor family is one of the largest groups of transcription factor in plants, playing important roles in growth, development, and biotic and abiotic stress responses. Many WRKY genes have been cloned from a variety of plant species and their functions have been analyzed. However, the studies on WRKY transcription factors in tree species under abiotic stress are still not well characterized. To understand the effects of the WRKY gene in response to abiotic stress, mRNA abundances of 102 WRKY genes in Populus simonii × P. nigra were identified by RNA sequencing under normal and salt stress conditions. The expression of 23 WRKY genes varied remarkably, in a tissue-specific manner, under salt stress. Since the WRKY56 was one of the genes significantly induced by NaCl treatment, its cDNA fragment containing an open reading frame from P. simonii × P. nigra was then cloned and transferred into Arabidopsis using the floral dip method. Under salt stress, the transgenic Arabidopsis over-expressed the WRKY56 gene, showing an increase in fresh weight, germination rate, proline content, and peroxidase and superoxide dismutase activity, when compared with the wild type. In contrast, transgenic Arabidopsis displayed a decrease in malondialdehyde content under salt stress. Overall, these results indicated that the WRKY56 gene played an important role in regulating salt tolerance in transgenic Arabidopsis.

DIFFERATATION OF WHEAT WRKY TRANSCRIPTION FACTOR TaWRKY10 GENE EXPRESSION IN ABIOTIC STRESS RESISTANCE

2015 ◽  
Vol 13 (2) ◽  
pp. 136-140 ◽  
Author(s):  
Odgerel Bold ◽  
R Jana Jeevan ◽  
Yong Pyo Lim ◽  
Enkhchimeg Vanjildorj
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Abiotic Stress ◽  
Triticum Aestivum L ◽  
Wrky Transcription Factor ◽  
Expression Level ◽  
Wheat Varieties ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Polymerase Chain

Drought, cold and salinity are the primary factors limiting wheat production. It has been shown that a wheat stress-responsive WRKY transcription factor encoded by the wheat WRKY10 gene plays an important role in drought, cold and salinty stress tolerance. The aim of the current study was to WRKY10 gene expression in drought, cold and salinity up-regulated in six varieties of Mongolian local wheat (Triticum aestivum L.), to select the stress tolerant best variety for breeding program. Plants expressing TaWRKY10 was up-regulated 48 hours stress by treatment with polyethylene glycol (PEG6000), 200 mM NaCl and 40C cold. Results of RT-PCR indicate that the all varieties show the gene expression level. Furthermore, reverse transcription polymerase chain reactions revealed that the expressions of WRKY 10 genes involved in abiotic stress signaling of six wheat varieties. These results indicated highest expression level of the WRKY10 in Darkhan-131 and lowest expression in Darkhan-34. And our study provided a promising approach to improve the tolerances of wheat cultivars to drought and salinity varieties of selection materials.Mongolian Journal of Agricultural Sciences Vol.13(2) 2014: 136-140

scholarly journals Expression of a Grape VqSTS36-Increased Resistance to Powdery Mildew and Osmotic Stress in Arabidopsis but Enhanced Susceptibility to Botrytis cinerea in Arabidopsis and Tomato

2018 ◽  
Vol 19 (10) ◽  
pp. 2985 ◽  
Author(s):  
Li Huang ◽  
Xiangjing Yin ◽  
Xiaomeng Sun ◽  
Jinhua Yang ◽  
Mohammad Rahman ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Powdery Mildew ◽  
Botrytis Cinerea ◽  
Transgenic Arabidopsis ◽  
Stilbene Synthase ◽  
Biotic And Abiotic Stress ◽  
Salt And Drought Stress ◽  
Insight Into ◽  
Increased Susceptibility

Stilbene synthase genes make a contribution to improving the tolerances of biotic and abiotic stress in plants. However, the mechanisms mediated by these STS genes remain unclear. To provide insight into the role of STS genes defense against biotic and abiotic stress, we overexpressed VqSTS36 in Arabidopsis thaliana and tomato (Micro-Tom) via Agrobacterium-mediated transformation. VqSTS36-transformed Arabidopsis lines displayed an increased resistance to powdery mildew, but both VqSTS36-transformed Arabidopsis and tomato lines showed the increased susceptibility to Botrytis cinerea. Besides, transgenic Arabidopsis lines were found to confer tolerance to salt and drought stress in seed and seedlings. When transgenic plants were treated with a different stress, qPCR assays of defense-related genes in transgenic Arabidopsis and tomato suggested that VqSTS36 played a specific role in different phytohormone-related pathways, including salicylic acid, jasmonic acid, and abscisic acid signaling pathways. All of these results provided a better understanding of the mechanism behind the role of VqSTS36 in biotic and abiotic stress.

scholarly journals Overexpression of an AP2/ERF Type Transcription Factor OsEREBP1 Confers Biotic and Abiotic Stress Tolerance in Rice

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0127831 ◽  
Author(s):  
V. Jisha ◽  
Lavanya Dampanaboina ◽  
Jyothilakshmi Vadassery ◽  
Axel Mithöfer ◽  
Saivishnupriya Kappara ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Biotic And Abiotic Stress
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