scholarly journals The GhMYB36 transcription factor confers resistance to biotic and abiotic stress by enhancing PR1 gene expression in plants

2021 ◽  
Author(s):  
Tingli Liu ◽  
Tianzi Chen ◽  
Jialiang Kan ◽  
Yao Yao ◽  
Dongshu Guo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Abiotic Stress ◽  
Biotic And Abiotic Stress

scholarly journals R2R3-MYB transcription factors, StmiR858 and sucrose mediate potato flavonol biosynthesis

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Sen Lin ◽  
Rajesh K. Singh ◽  
Moehninsi ◽  
Duroy A. Navarre
Keyword(s):  
Gene Expression ◽  
Abiotic Stress ◽  
Feedback Loop ◽  
Regulatory Mechanisms ◽  
Phenylpropanoid Metabolism ◽  
Biotic And Abiotic Stress ◽  
Health Promoting ◽  
Transient Overexpression ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors

AbstractFlavonols and other phenylpropanoids protect plants from biotic and abiotic stress and are dietarily desirable because of their health-promoting properties. The ability to develop new potatoes (Solanum tuberosum) with optimal types and amounts of phenylpropanoids is limited by lack of knowledge about the regulatory mechanisms. Exogenous sucrose increased flavonols, whereas overexpression of the MYB StAN1 induced sucrolytic gene expression. Heterologous StAN1 protein bound promoter fragments from sucrolytic genes (SUSY1 and INV1). Two additional MYBs and one microRNA were identified that regulated potato flavonols. Overexpression analysis showed MYB12A and C increased amounts of flavonols and other phenylpropanoids. Endogenous flavonol amounts in light-exposed organs were much higher those in the dark. Expression levels of StMYB12A and C were high in flowers but low in tubers. Transient overexpression of miR858 altered potato flavonol metabolism. Endogenous StmiR858 expression was much lower in flowers than leaves and correlated with flavonol amounts in these organs. Collectively, these findings support the hypothesis that sucrose, MYBs, and miRNA control potato phenylpropanoid metabolism in a finely tuned manner that includes a feedback loop between sucrose and StAN1. These findings will aid in the development of potatoes with phenylpropanoid profiles optimized for crop performance and human health.

scholarly journals Transcriptome-based identification and validation of optimal reference genes for quantitative real-time PCR normalization in Psathyrostachys huashanica

2020 ◽  
Author(s):  
Chuan Shen ◽  
Jingyuan Li ◽  
Caiyan Wei ◽  
Xudong Zhang ◽  
Yunfeng Wu
Keyword(s):  
Gene Expression ◽  
Abiotic Stress ◽  
Real Time ◽  
Temperature Stress ◽  
Reference Genes ◽  
18S Rrna ◽  
Stress Conditions ◽  
Psathyrostachys Huashanica ◽  
Biotic And Abiotic Stress ◽  
Ubiquitin Conjugating Enzyme

Abstract Background: P. huashanica ( Psathyrostachys huashanica ), known as an important resistance resource reservoir, is a rare and endangered plant growing suitably in Huashan mount region and would be urgently exploited in wheat genetic improvements sooner. During the utilization process, different IRGs (internal reference genes) need to be appropriately selected as standards based on biotic and abiotic stress conditions. It is crucial that Real-time RT-qPCR with combination of bioinformatics were adopted to explore the reliable IRGs from transcriptome of P . huashanica.Results: The present work reported new 3 species of IRGs, UBC2 , UBC17, 18S rRNA , which were screened from transcriptome of P. huashanica under biotic and abiotic stress conditions, using RT-qPCR and four algorithms, including geNorm, NormFinder, BestKeeper, and RefFinder, to analyse expression of sixteen candidate reference genes. These genes appear as following 18S rRNA (18S ribosomal RNA), EF1-α (eukaryotic elongation factor 1 alpha), UBC2 (ubiquitin-conjugating enzyme E2-2), UBC17 (ubiquitin-conjugating enzyme E2-17), α-TUB2A (alpha tubulin-2A), β-TUB3 (beta tubulin 3), ADF4 (Actin-depolymerising factor 4), ACTIN (actin), GAPDH (Glyceraldehyde-3-phosphate dehydrogenase), 60SARP (60S acidic ribosomal protein), UBQ (polyubiquitin), SamDC (S-Adenosylmethionine decarboxylase), EIF4A (eukaryotic initiation factor 4A), ARF (ADP-ribosylation factor), HIS1 (histone H1), and HIS2B (histone H2B). Analysis of gene expression demonstrated that the expression of UBC2 gene was most stable under ABA hormone stress, low temperature stress and high temperature stress, similarly, UBC17 gene under IAA hormone stress, salinity stress and drought stress, both UBC17 genes and 18S rRNA genes under abiotic and biotic stress, respectively. The most stable gene was UBC2 gene in the root, UBC17 gene in stem and leaf. In this study, α-TUB2A , UBC and ACTIN genes were verified as the suitable reference genes across all tested samples. To further validate the suitability of the selected reference genes, we evaluated the relative expression of PsaCPK3 (Calcium-dependent protein kinase) and PsaHSP70-1 (heat shock protein 70-1), which are stress-related genes that may be involved in response to adversity.Conclusions: This study has identified a set of the most stable IRGs suiting for RT-qPCR detection of a few target gene expressions from P . huashanica in different experimental conditions. In addition, this study should provide the accuracy information for gene expression analysis in P . huashanica .

scholarly journals Association of transcription factor WRKY56 gene from Populus simonii × P. nigra with salt tolerance in Arabidopsis thaliana

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7291 ◽  
Author(s):  
Lei Wang ◽  
Wenjing Yao ◽  
Yao Sun ◽  
Jiying Wang ◽  
Tingbo Jiang
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Germination Rate ◽  
Wrky Transcription Factor ◽  
Biotic And Abiotic Stress ◽  
Populus Simonii ◽  
Reading Frame

The WRKY transcription factor family is one of the largest groups of transcription factor in plants, playing important roles in growth, development, and biotic and abiotic stress responses. Many WRKY genes have been cloned from a variety of plant species and their functions have been analyzed. However, the studies on WRKY transcription factors in tree species under abiotic stress are still not well characterized. To understand the effects of the WRKY gene in response to abiotic stress, mRNA abundances of 102 WRKY genes in Populus simonii × P. nigra were identified by RNA sequencing under normal and salt stress conditions. The expression of 23 WRKY genes varied remarkably, in a tissue-specific manner, under salt stress. Since the WRKY56 was one of the genes significantly induced by NaCl treatment, its cDNA fragment containing an open reading frame from P. simonii × P. nigra was then cloned and transferred into Arabidopsis using the floral dip method. Under salt stress, the transgenic Arabidopsis over-expressed the WRKY56 gene, showing an increase in fresh weight, germination rate, proline content, and peroxidase and superoxide dismutase activity, when compared with the wild type. In contrast, transgenic Arabidopsis displayed a decrease in malondialdehyde content under salt stress. Overall, these results indicated that the WRKY56 gene played an important role in regulating salt tolerance in transgenic Arabidopsis.

DIFFERATATION OF WHEAT WRKY TRANSCRIPTION FACTOR TaWRKY10 GENE EXPRESSION IN ABIOTIC STRESS RESISTANCE

2015 ◽  
Vol 13 (2) ◽  
pp. 136-140 ◽  
Author(s):  
Odgerel Bold ◽  
R Jana Jeevan ◽  
Yong Pyo Lim ◽  
Enkhchimeg Vanjildorj
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Abiotic Stress ◽  
Triticum Aestivum L ◽  
Wrky Transcription Factor ◽  
Expression Level ◽  
Wheat Varieties ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Polymerase Chain

Drought, cold and salinity are the primary factors limiting wheat production. It has been shown that a wheat stress-responsive WRKY transcription factor encoded by the wheat WRKY10 gene plays an important role in drought, cold and salinty stress tolerance. The aim of the current study was to WRKY10 gene expression in drought, cold and salinity up-regulated in six varieties of Mongolian local wheat (Triticum aestivum L.), to select the stress tolerant best variety for breeding program. Plants expressing TaWRKY10 was up-regulated 48 hours stress by treatment with polyethylene glycol (PEG6000), 200 mM NaCl and 40C cold. Results of RT-PCR indicate that the all varieties show the gene expression level. Furthermore, reverse transcription polymerase chain reactions revealed that the expressions of WRKY 10 genes involved in abiotic stress signaling of six wheat varieties. These results indicated highest expression level of the WRKY10 in Darkhan-131 and lowest expression in Darkhan-34. And our study provided a promising approach to improve the tolerances of wheat cultivars to drought and salinity varieties of selection materials.Mongolian Journal of Agricultural Sciences Vol.13(2) 2014: 136-140

scholarly journals Strawberry FaWRKY25 Transcription Factor Negatively Regulated the Resistance of Strawberry Fruits to Botrytis cinerea

Genes ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 56
Author(s):  
Sizhen Jia ◽  
Yuanhua Wang ◽  
Geng Zhang ◽  
Zhiming Yan ◽  
Qingsheng Cai
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Jasmonic Acid ◽  
Botrytis Cinerea ◽  
Crucial Role ◽  
Defense Mechanism ◽  
Resistance Mechanism ◽  
Expression Level ◽  
Biotic And Abiotic Stress ◽  
Strawberry Resistance

WRKY genes and jasmonic acid (JA) play a crucial role in plants’ responses against biotic and abiotic stress. However, the regulating mechanism of WRKY genes on strawberry fruits’ resistance against Botrytis cinerea is largely unknown, and few studies have been performed on their effect on the JA-mediated defense mechanism against B. cinerea. This study explored the effect of FaWRKY25 on the JA-mediated strawberry resistance against B. cinerea. Results showed that the JA content decreased significantly as the fruits matured, whereas the FaWRKY25 expression rose substantially, which led to heightened susceptibility to B. cinerea and in strawberries. External JA treatment significantly increased the JA content in strawberries and reduced the FaWRKY25 expression, thereby enhancing the fruits’ resistance against B. cinerea. FaWRKY25 overexpression significantly lowered the fruits’ resistance against B. cinerea, whereas FaWRKY25 silencing significantly increased resistance. Moreover, FaWRKY25 overexpression significantly lowered the JA content, whereas FaWRKY25 silencing significantly increased it. FaWRKY25 expression level substantially affects the expression levels of genes related to JA biosynthesis and metabolism, other members of the WRKY family, and defense genes. Accordingly, FaWRKY25 plays a crucial role in regulating strawberries’ resistance against B. cinerea and may negatively regulate their JA-mediated resistance mechanism against B. cinerea.

Sign in / Sign up

Export Citation Format

Share Document