scholarly journals Comparative Analysis of the Transcriptome and Distribution of Putative SNPs in Two Rainbow Trout (Oncorhynchus mykiss) Breeding Strains by Using Next-Generation Sequencing

Genes ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 841 ◽  
Author(s):  
Lidia de los Ríos-Pérez ◽  
Ronald Marco Brunner ◽  
Frieder Hadlich ◽  
Alexander Rebl ◽  
Carsten Kühn ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Gene Networks ◽  
Expression Patterns ◽  
High Stress ◽  
Head Kidney ◽  
Fish Farming ◽  
Specific Gene ◽  
Nucleotide Polymorphisms ◽  
Rainbow Trout Oncorhynchus Mykiss

Selective breeding can significantly improve the establishment of sustainable and profitable aquaculture fish farming. For rainbow trout (Oncorhynchus mykiss), one of the main aquaculture coldwater species in Europe, a variety of selected hatchery strains are commercially available. In this study, we investigated the genetic variation between the local Born strain, selected for survival, and the commercially available Silver Steelhead strain, selected for growth. We sequenced the transcriptome of six tissues (gills, head kidney, heart, liver, spleen, and white muscle) from eight healthy individuals per strain, using RNA-seq technology to identify strain-specific gene-expression patterns and single nucleotide polymorphisms (SNPs). In total, 1760 annotated genes were differentially expressed across all tissues. Pathway analysis assigned them to different gene networks. We also identified a set of SNPs, which are heterozygous for one of the two breeding strains: 1229 of which represent polymorphisms over all tissues and individuals. Our data indicate a strong genetic differentiation between Born and Silver Steelhead trout, despite the relatively short time of evolutionary separation of the two breeding strains. The results most likely reflect their specifically adapted genotypes and might contribute to the understanding of differences regarding their robustness toward high stress and pathogenic challenge described in former studies.

scholarly journals Optimisation of the lymphocyte proliferation assay in rainbow trout (Oncorhynchus mykiss)

2019 ◽  
Vol 64 (No. 12) ◽  
pp. 547-557
Author(s):  
H Minarova ◽  
M Palikova ◽  
J Mares ◽  
E Syrova ◽  
J Blahova ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Bovine Serum ◽  
Lymphocyte Proliferation ◽  
Head Kidney ◽  
Pokeweed Mitogen ◽  
Lymphocyte Proliferation Assay ◽  
Proliferation Assay ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Foetal Bovine Serum

The lymphocyte proliferation assay is a valuable method used for the evaluation of the fish immune system. However, there are many variations and optimal results are not always obtained. Unification is necessary to ensure the comparability between different studies. The aim of this study was to optimise the lymphocyte proliferation assay in rainbow trout (Oncorhynchus mykiss). This goal included the determination of the optimal incubation length, serum type, incubation temperature, type of mitogen and its concentration, and anticoagulant. The peripheral blood and head kidney lymphocytes were isolated by density gradient centrifugation. Subsequently, the cells were incubated for 3–8 days with different mitogens (pokeweed mitogen 5, 10 and 50 µg/ml, concanavalin A 1, 10 and 20 µg/ml, phytohaemagglutinin 25, 50 and 100 µg/ml, lipopolysaccharide 1, 50 and 100 µg/ml). The use of the different serum types (foetal bovine serum, trout serum), incubation temperatures (10–20 °C) and anticoagulants (heparin, EDTA) was compared. Labelled thymidine was used to evaluate the assay. The best results were obtained after seven days of incubation at 15 °C with foetal bovine serum (FBS). The head kidney lymphocytes showed the highest proliferative response with 50 µg/ml phytohaemagglutinin. With the peripheral blood lymphocytes (heparin and EDTA), the best results were obtained with 50 µg/ml pokeweed mitogen. The highest proliferation levels were detected with heparinised blood. In conclusion, optimisation of this assay contributes to the improved assessment of the rainbow trout immune function.

Daily Rhythmic Expression Patterns ofClock1a,Bmal1, andPer1Genes in Retina and Hypothalamus of the Rainbow Trout,Oncorhynchus Mykiss

2011 ◽  
Vol 28 (5) ◽  
pp. 381-389 ◽  
Author(s):  
Marcos A. López Patiño ◽  
Arnau Rodríguez-Illamola ◽  
Marta Conde-Sieira ◽  
José L. Soengas ◽  
Jesús M. Míguez
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Expression Patterns ◽  
Rhythmic Expression ◽  
Rainbow Trout Oncorhynchus Mykiss

Transcriptomic responses to heat stress in rainbow trout Oncorhynchus mykiss head kidney

2018 ◽  
Vol 82 ◽  
pp. 32-40 ◽  
Author(s):  
Jinqiang Huang ◽  
Yongjuan Li ◽  
Zhe Liu ◽  
Yujun Kang ◽  
Jianfu Wang
Keyword(s):  
Heat Stress ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Head Kidney ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Transcriptomic Responses

Depletion of selected polychlorinated biphenyl, dibenzodioxin, and dibenzofuran congeners in farmed rainbow trout (Oncorhynchus mykiss): A hint for safer fish farming

Chemosphere ◽  
2007 ◽  
Vol 66 (6) ◽  
pp. 1019-1030 ◽  
Author(s):  
Gianfranco Brambilla ◽  
Elena Dellatte ◽  
Igor Fochi ◽  
Nicola Iacovella ◽  
Roberto Miniero ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Polychlorinated Biphenyl ◽  
Fish Farming ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals 11-Deoxycorticosterone Is a Potent Agonist of the Rainbow Trout (Oncorhynchus mykiss) Mineralocorticoid Receptor

Endocrinology ◽  
2005 ◽  
Vol 146 (1) ◽  
pp. 47-55 ◽  
Author(s):  
A. Sturm ◽  
N. Bury ◽  
L. Dengreville ◽  
J. Fagart ◽  
G. Flouriot ◽  
...  
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Mineralocorticoid Receptor ◽  
Head Kidney ◽  
Luciferase Reporter ◽  
Similar Response ◽  
Hormonal Stimulation ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Juvenile Trout

The teleost fish are thought to lack the mineralocorticoid hormone aldosterone but possess mineralocorticoid receptor (MR) homologs. Here we describe the characterization of two rainbow trout (Oncorhynchus mykiss) MRs, called rtMRa and rtMRb. The open reading frame of rtMRa cDNA encoded a protein of 1041 amino acids. The rtMRb predicted protein sequence is similar, differing in only 10 amino acids in the nonconserved A/B domain and lacking a three-amino acid insertion between the two zinc fingers of the C domain. Expression of rtMR mRNA (sum of both forms), measured in juvenile trout by real-time RT-PCR, shows that the transcripts are ubiquitous. Expression was significantly higher in brain than the other tissues studied (eye, trunk kidney, head kidney, gut, gills, liver, spleen, ovary, heart, white muscle, skin). Hormonal stimulation of receptor transactivation activity was studied in COS-7 cells transiently cotransfected with receptor cDNA and a mouse mammary tumor virus-luciferase reporter. The mineralocorticoids 11-deoxycorticosterone and aldosterone were more potent enhancers of rtMRa transcriptional activity (EC50 = 1.6 ± 0.5 × 10−10 and 1.1 ± 0.4 × 10−10m, respectively) than the glucocorticoids cortisol and 11-deoxycortisol (EC50 = 1.1 ± 0.3 × 10−9 and 3.7 ± 1.9 × 10−9m, respectively). A similar response was observed in transactivation assays with rtMRb. These results are discussed in the view of reported circulating levels of corticosteroids in trout.

scholarly journals Draft Genome Sequence of Epilithonimonas sp. FP211-J200, Isolated from an Outbreak Episode on a Rainbow Trout (Oncorhynchus mykiss) Farm

2017 ◽  
Vol 5 (37) ◽  
Author(s):  
Manuel Ayala ◽  
Cristopher Segovia ◽  
Rodrigo Rojas ◽  
Claudio Miranda ◽  
Javier Santander
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Genome Sequence ◽  
Draft Genome ◽  
Head Kidney ◽  
Fish Host ◽  
Draft Genome Sequence ◽  
Kidney Cells ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Head Kidney Cells

ABSTRACT Here, we report the draft genome sequence of Epilithonimonas sp. FP211-J200, isolated from rainbow trout head kidney cells. The size of the genome is 4,110,772 bp, with a G+C content of 37.1%. The Epilithonimonas sp. FP211-J200 genome has genes related to tetracycline and β-lactam resistance. This is the first reported Epilithonimonas species genome isolated from a fish host.

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