scholarly journals Cyclopamine Induced Expression of Immune-related Genes in Rainbow trout (Oncorhynchus mykiss) Head Kidney Leukocytes

2018 ◽  
Author(s):  
Y.K. zdemit ◽  
S. Bilen ◽  
R.C. Özdemir ◽  
A.Y. Sõnmev
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Head Kidney ◽  
Induced Expression ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Immune Related Genes

PAMP induced expression of immune relevant genes in head kidney leukocytes of rainbow trout (Oncorhynchus mykiss)

2011 ◽  
Vol 35 (4) ◽  
pp. 476-482 ◽  
Author(s):  
Jiwan K. Chettri ◽  
Martin K. Raida ◽  
Lars Holten-Andersen ◽  
Per W. Kania ◽  
Kurt Buchmann
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Head Kidney ◽  
Induced Expression ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals The Early Immune Response of Lymphoid and Myeloid Head-Kidney Cells of Rainbow Trout (Oncorhynchus mykiss) Stimulated with Aeromonas salmonicida

Fishes ◽  
2022 ◽  
Vol 7 (1) ◽  
pp. 12
Author(s):  
Fabio Sarais ◽  
Ruth Montero ◽  
Sven Ostermann ◽  
Alexander Rebl ◽  
Bernd Köllner ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Gene Expression Pattern ◽  
Myeloid Cells ◽  
Head Kidney ◽  
Aeromonas Salmonicida ◽  
Adaptive Immune ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Cell Subpopulations ◽  
Immune Related Genes ◽  
Head Kidney Cells

The teleost head kidney is a highly relevant immune organ, and myeloid cells play a major role in this organ’s innate and adaptive immune responses. Because of their complexity, the early phases of the innate immune reaction of fish against bacteria are still poorly understood. In this study, naïve rainbow trout were stimulated with inactivated A. salmonicida and sampled at 12 h, 24 h and 7 d poststimulation. Cells from the head kidney were magnetically sorted with a monoclonal antibody mAB21 to obtain one (MAb21-positive) fraction enriched with myeloid cells and one (MAb21-negative) fraction enriched with lymphocytes and thrombocytes. The gene expression pattern of the resulting cell subpopulations was analysed using a panel of 43 immune-related genes. The results show an overall downregulation of the complement pathway and cytokine production at the considered time points. Some of the selected genes may be considered as parameters for diagnosing bacterial furunculosis of rainbow trout.

scholarly journals Optimisation of the lymphocyte proliferation assay in rainbow trout (Oncorhynchus mykiss)

2019 ◽  
Vol 64 (No. 12) ◽  
pp. 547-557
Author(s):  
H Minarova ◽  
M Palikova ◽  
J Mares ◽  
E Syrova ◽  
J Blahova ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Bovine Serum ◽  
Lymphocyte Proliferation ◽  
Head Kidney ◽  
Pokeweed Mitogen ◽  
Lymphocyte Proliferation Assay ◽  
Proliferation Assay ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Foetal Bovine Serum

The lymphocyte proliferation assay is a valuable method used for the evaluation of the fish immune system. However, there are many variations and optimal results are not always obtained. Unification is necessary to ensure the comparability between different studies. The aim of this study was to optimise the lymphocyte proliferation assay in rainbow trout (Oncorhynchus mykiss). This goal included the determination of the optimal incubation length, serum type, incubation temperature, type of mitogen and its concentration, and anticoagulant. The peripheral blood and head kidney lymphocytes were isolated by density gradient centrifugation. Subsequently, the cells were incubated for 3–8 days with different mitogens (pokeweed mitogen 5, 10 and 50 µg/ml, concanavalin A 1, 10 and 20 µg/ml, phytohaemagglutinin 25, 50 and 100 µg/ml, lipopolysaccharide 1, 50 and 100 µg/ml). The use of the different serum types (foetal bovine serum, trout serum), incubation temperatures (10–20 °C) and anticoagulants (heparin, EDTA) was compared. Labelled thymidine was used to evaluate the assay. The best results were obtained after seven days of incubation at 15 °C with foetal bovine serum (FBS). The head kidney lymphocytes showed the highest proliferative response with 50 µg/ml phytohaemagglutinin. With the peripheral blood lymphocytes (heparin and EDTA), the best results were obtained with 50 µg/ml pokeweed mitogen. The highest proliferation levels were detected with heparinised blood. In conclusion, optimisation of this assay contributes to the improved assessment of the rainbow trout immune function.

Transcriptomic responses to heat stress in rainbow trout Oncorhynchus mykiss head kidney

2018 ◽  
Vol 82 ◽  
pp. 32-40 ◽  
Author(s):  
Jinqiang Huang ◽  
Yongjuan Li ◽  
Zhe Liu ◽  
Yujun Kang ◽  
Jianfu Wang
Keyword(s):  
Heat Stress ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Head Kidney ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Transcriptomic Responses

scholarly journals 11-Deoxycorticosterone Is a Potent Agonist of the Rainbow Trout (Oncorhynchus mykiss) Mineralocorticoid Receptor

Endocrinology ◽  
2005 ◽  
Vol 146 (1) ◽  
pp. 47-55 ◽  
Author(s):  
A. Sturm ◽  
N. Bury ◽  
L. Dengreville ◽  
J. Fagart ◽  
G. Flouriot ◽  
...  
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Mineralocorticoid Receptor ◽  
Head Kidney ◽  
Luciferase Reporter ◽  
Similar Response ◽  
Hormonal Stimulation ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Juvenile Trout

The teleost fish are thought to lack the mineralocorticoid hormone aldosterone but possess mineralocorticoid receptor (MR) homologs. Here we describe the characterization of two rainbow trout (Oncorhynchus mykiss) MRs, called rtMRa and rtMRb. The open reading frame of rtMRa cDNA encoded a protein of 1041 amino acids. The rtMRb predicted protein sequence is similar, differing in only 10 amino acids in the nonconserved A/B domain and lacking a three-amino acid insertion between the two zinc fingers of the C domain. Expression of rtMR mRNA (sum of both forms), measured in juvenile trout by real-time RT-PCR, shows that the transcripts are ubiquitous. Expression was significantly higher in brain than the other tissues studied (eye, trunk kidney, head kidney, gut, gills, liver, spleen, ovary, heart, white muscle, skin). Hormonal stimulation of receptor transactivation activity was studied in COS-7 cells transiently cotransfected with receptor cDNA and a mouse mammary tumor virus-luciferase reporter. The mineralocorticoids 11-deoxycorticosterone and aldosterone were more potent enhancers of rtMRa transcriptional activity (EC50 = 1.6 ± 0.5 × 10−10 and 1.1 ± 0.4 × 10−10m, respectively) than the glucocorticoids cortisol and 11-deoxycortisol (EC50 = 1.1 ± 0.3 × 10−9 and 3.7 ± 1.9 × 10−9m, respectively). A similar response was observed in transactivation assays with rtMRb. These results are discussed in the view of reported circulating levels of corticosteroids in trout.

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