scholarly journals Systematic Analysis of Kelch Repeat F-box (KFB) Protein Family and Identification of Phenolic Acid Regulation Members in Salvia miltiorrhiza Bunge

Genes ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 557
Author(s):  
Haizheng Yu ◽  
Mengdan Jiang ◽  
Bingcong Xing ◽  
Lijun Liang ◽  
Bingxue Zhang ◽  
...  
Keyword(s):  
Secondary Metabolism ◽  
Phenolic Acids ◽  
Salvia Miltiorrhiza ◽  
Chinese Herb ◽  
Cerebrovascular Diseases ◽  
Regulation Mechanism ◽  
Salvia Miltiorrhiza Bunge ◽  
Specific Expression ◽  
Systematic Analysis ◽  
Meja Treatment

S. miltiorrhiza is a well-known Chinese herb for the clinical treatment of cardiovascular and cerebrovascular diseases. Tanshinones and phenolic acids are the major secondary metabolites and significant pharmacological constituents of this plant. Kelch repeat F-box (KFB) proteins play important roles in plant secondary metabolism, but their regulation mechanism in S. miltiorrhiza has not been characterized. In this study, we systematically characterized the S. miltiorrhiza KFB gene family. In total, 31 SmKFB genes were isolated from S. miltiorrhiza. Phylogenetic analysis of those SmKFBs indicated that 31 SmKFBs can be divided into four groups. Thereinto, five SmKFBs (SmKFB1, 2, 3, 5, and 28) shared high homology with other plant KFBs which have been described to be regulators of secondary metabolism. The expression profile of SmKFBs under methyl jasmonate (MeJA) treatment deciphered that six SmKFBs (SmKFB1, 2, 5, 6, 11, and 15) were significantly downregulated, and two SmKFBs (SmKFB22 and 31) were significantly upregulated. Tissue-specific expression analysis found that four SmKFBs (SmKFB4, 11, 16, and 17) were expressed preferentially in aerial tissues, while two SmKFBs (SmKFB5, 25) were predominantly expressed in roots. Through a systematic analysis, we speculated that SmKFB1, 2, and 5 are potentially involved in phenolic acids biosynthesis.

scholarly journals Overview of Salvia miltiorrhiza as a Potential Therapeutic Agent for Various Diseases: An Update on Efficacy and Mechanisms of Action

Antioxidants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 857
Author(s):  
Inyong Jung ◽  
Hyerin Kim ◽  
Seongcheol Moon ◽  
Hyuk Lee ◽  
Bonglee Kim
Keyword(s):  
Oxidative Stress ◽  
Therapeutic Agent ◽  
Salvia Miltiorrhiza ◽  
Cerebrovascular Diseases ◽  
Mechanisms Of Action ◽  
Therapeutic Effects ◽  
Nervous System Diseases ◽  
Salvia Miltiorrhiza Bunge ◽  
Pubmed Central ◽  
Therapeutic Benefits

Salvia miltiorrhiza Bunge (S. miltiorrhiza) is a medicinal herb that has been used for the treatment for various diseases such as cardiovascular and cerebrovascular diseases in East Asia including Korea. Considering its extensive usage as a therapeutic agent for multiple diseases, there is a need to review previous research regarding its therapeutic benefits and their mechanisms. Therefore, we searched PubMed and PubMed Central for articles reporting its therapeutic effects on certain disease groups including cancers, cardiovascular, liver, and nervous system diseases. This review provides an overview of therapeutic benefits and targets of S. miltiorrhiza, including inflammation, fibrosis, oxidative stress, and apoptosis. The findings on multi-functional properties of S. miltiorrhiza discussed in this article support the efficacy of S. miltiorrhiza extract on various diseases, but also call for further research on the multiple mechanisms that mediate its therapeutic effects.

scholarly journals Diterpenoid Tanshinones and Phenolic Acids from Cultured Hairy Roots of Salvia miltiorrhiza Bunge and Their Antimicrobial Activities

Molecules ◽  
2011 ◽  
Vol 16 (3) ◽  
pp. 2259-2267 ◽  
Author(s):  
Jianglin Zhao ◽  
Jingfeng Lou ◽  
Yan Mou ◽  
Peiqin Li ◽  
Jianyong Wu ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Hairy Roots ◽  
Salvia Miltiorrhiza ◽  
Antimicrobial Activities ◽  
Salvia Miltiorrhiza Bunge

scholarly journals Transcriptomes Analysis Reveals the Regulatory Roles of Noncoding RNA in Tanshinones Synthesis Pathway of Salvia Miltiorrhiza

2021 ◽  
Author(s):  
Caicai Lin ◽  
Changhao Zhou ◽  
Zhongqian Liu ◽  
Xingfeng Li ◽  
Zhenqiao Song
Keyword(s):  
Noncoding Rna ◽  
Target Genes ◽  
Salvia Miltiorrhiza ◽  
Expression Patterns ◽  
Noncoding Rnas ◽  
Synthesis Process ◽  
Circular Rnas ◽  
Regulation Mechanism ◽  
Pentatricopeptide Repeat ◽  
Specific Expression

Abstract Background: Long noncoding RNAs (lncRNAs), circular RNAs (circRNAs), and microRNAs (miRNAs) have been shown to play fundamental roles in plant development. However, the information of these noncoding RNAs (ncRNAs) in Salvia miltiorrhiza remains largely unexplored. In this study, the expression pattern of ncRNAs in six tissues from the same strain of S. miltiorrhiza was analyzed to study the biological function of ncRNAs on active ingredients synthesis.Methods: Analysis of tanshinone content differences of two root simples was carried out on high-performance liquid chromatography (HPLC). RNA sequencing, GO and KEGG enrichment analysis were applied to analyzing the targets of diferentially expressed ncRNAs in different organs.Results: A total of 6,929 lncRNAs, 6,239 circRNAs, and 360 miRNAs were identified. Forty-eight lncRNAs, 70 miRNAs, and 26 circRNAs expressed differentially between red and white root tissues with significantly different tanshinone content. GO and KEGG pathway analysis of target genes of differently expressed ncRNAs indicated that some target genes are involved in the synthesis pathway of terpene, including diterpene and sesquiterpene. We also found many target genes related to secondary metabolites, including 2-C-Methyl-d-erythritol 2,4-cyclodiphosphate Synthase (SmMCS) and several CYP450s. Furthermore, most target genes may be related to the resistance of pathogens, such as receptor kinases, disease-resistant proteins, and pentatricopeptide repeat-containing proteins. Conclusions: The present study exhibited the tissue-specific expression patterns of ncRNAs preliminarily in S. miltiorrhiza, which may reflect that the formation of white root or red root is related to regulation by ncRNAs. It would provide a basis for further research about the regulation mechanism in the tanshinone synthesis process.

scholarly journals The effect of mineral fertilization on the content of phenolic acids and mass of roots of Salvia miltiorrhiza Bunge

2013 ◽  
Vol 57 (1-2) ◽  
pp. 169-173
Author(s):  
Waldemar Buchwald
Keyword(s):  
Phenolic Acids ◽  
Salvia Miltiorrhiza ◽  
Dry Mass ◽  
Pure Component ◽  
Average Content ◽  
Atmospheric Conditions ◽  
Salvia Miltiorrhiza Bunge ◽  
Mineral Fertilization ◽  
Component Content ◽  
One Year

Research on the mineral fertilization of Salvia miltiorrhiza Bunge was carried out in the years 2000 - 2002. It was stated that the potassium fertilization has the strongest effect on the content of phenolic acids and on mass of roots of <i>Salvia miltiorrhiza</i>. The effect of atmospheric conditions in particular years is also essential. The optimal doses of fertilizers (pure component content in kg · ha<sup>-</sup>) were the following: N - 100, P<sub>2</sub>O<sub>5</sub> - 30, K<sub>2</sub>0 - 180. Having used this fertilizing combination the average content of phenolic acids was 4,62% and the average aerial dry mass of roots was 13,21 g per 1 plant after one year of cultivation.

Quantitative determination and validation of four phenolic acids in Salvia Miltiorrhiza Bunge using 1H-NMR spectroscopy

2019 ◽  
Vol 16 ◽  
Author(s):  
Zhuoni Hou ◽  
Zongsuo Liang ◽  
Yuanyuan Li ◽  
Feng Su ◽  
Jipeng Chen ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
1H Nmr ◽  
Phenolic Acids ◽  
Rosmarinic Acid ◽  
1H Nmr Spectroscopy ◽  
Salvia Miltiorrhiza ◽  
Salvianolic Acid B ◽  
Salvia Miltiorrhiza Bunge ◽  
Salvianolic Acid ◽  
Root Extracts

Background: Although chromatography and spectrometry based methods have been used to analyse phenolic acids in Chinese traditional medicine Salvia miltiorrhiza Bunge (SMB), quantitative nuclear magnetic resonance (qNMR) has never previously been used to analyse fresh SMB root extracts. Objective: To establish a fast and simple method of quantitating danshensu, lithospermic acid, rosmarinic acid and salvianolic acid B content in fresh SMB root using 1H-NMR spectroscopy. Method: Fresh SMB root were extracted using a 70% methanol aqueous solution and quantitatively analysed for danshensu, lithospermic acid, rosmarinic acid and salvianolic acid B using 1H-NMR spectroscopy. Different internal standards were compared and the results were validated using high performance liquid chromatography. Results: The established method was accurate and precise with good recovery. The LOD and LOQ indicated the excellent sensitivity of the method. The robustness was testified by modification of four different parameters, and the differences among each parameter were all less than 2%. Conclusion: qNMR offers a fast, reliable and accurate method of identifying and quantifying danshensu, lithospermic acid, rosmarinic acid and salvianolic acid B in fresh SMB root extracts.

Downregulation of Cinnamoyl CoA Reductase Affects Lignin and Phenolic Acids Biosynthesis in Salvia miltiorrhiza Bunge

2012 ◽  
Vol 30 (5) ◽  
pp. 1229-1236 ◽  
Author(s):  
Zhengjun Wang ◽  
Langjun Cui ◽  
Chen Chen ◽  
Xiaojing Liu ◽  
Yaping Yan ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Salvia Miltiorrhiza ◽  
Salvia Miltiorrhiza Bunge ◽  
Cinnamoyl Coa Reductase ◽  
Coa Reductase

scholarly journals Plasma Membrane H+-ATPase SmPHA4 Negatively Regulates the Biosynthesis of Tanshinones in Salvia miltiorrhiza

2021 ◽  
Vol 22 (7) ◽  
pp. 3353
Author(s):  
Xiuhong Li ◽  
Bin Zhang ◽  
Pengda Ma ◽  
Ruizhi Cao ◽  
Xiaobing Yang ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Salvia Miltiorrhiza ◽  
Pharmacological Action ◽  
Cerebrovascular Diseases ◽  
Salvia Miltiorrhiza Bunge ◽  
Active Components ◽  
Phylogenetic Tree Analysis ◽  
Physiological Processes ◽  
Atpase Gene ◽  
Candidate Regulator

Salvia miltiorrhiza Bunge has been widely used in the treatment of cardiovascular and cerebrovascular diseases, due to the pharmacological action of its active components such as the tanshinones. Plasma membrane (PM) H+-ATPase plays key roles in numerous physiological processes in plants. However, little is known about the PM H+-ATPase gene family in S. miltiorrhiza (Sm). Here, nine PM H+-ATPase isoforms were identified and named SmPHA1–SmPHA9. Phylogenetic tree analysis showed that the genetic distance of SmPHAs was relatively far in the S. miltiorrhiza PM H+-ATPase family. Moreover, the transmembrane structures were rich in SmPHA protein. In addition, SmPHA4 was found to be highly expressed in roots and flowers. HPLC revealed that accumulation of dihydrotanshinone (DT), cryptotanshinone (CT), and tanshinone I (TI) was significantly reduced in the SmPHA4-OE lines but was increased in the SmPHA4-RNAi lines, ranging from 2.54 to 3.52, 3.77 to 6.33, and 0.35 to 0.74 mg/g, respectively, suggesting that SmPHA4 is a candidate regulator of tanshinone metabolites. Moreover, qRT-PCR confirmed that the expression of tanshinone biosynthetic-related key enzymes was also upregulated in the SmPHA4-RNAi lines. In summary, this study highlighted PM H+-ATPase function and provided new insights into regulatory candidate genes for modulating secondary metabolism biosynthesis in S. miltiorrhiza.

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