scholarly journals Expression Profile of Laccase Gene Family in White-Rot Basidiomycete Lentinula edodes under Different Environmental Stresses

Genes ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 1045
Author(s):  
Lianlian Yan ◽  
Ruiping Xu ◽  
Yinbing Bian ◽  
Hongxian Li ◽  
Yan Zhou
Keyword(s):  
Gene Family ◽  
Fruiting Body ◽  
Lentinula Edodes ◽  
Expression Profiles ◽  
Draft Genome ◽  
White Rot ◽  
Laccase Gene ◽  
Fruiting Body Formation ◽  
Body Formation ◽  
Laccase Genes

Laccases belong to ligninolytic enzymes and play important roles in various biological processes of filamentous fungi, including fruiting-body formation and lignin degradation. The process of fruiting-body development in Lentinula edodes is complex and is greatly affected by environmental conditions. In this paper, 14 multicopper oxidase-encoding (laccase) genes were analyzed in the draft genome sequence of L. edodes strain W1-26, followed by a search of multiple stress-related Cis-elements in the promoter region of these laccase genes, and then a transcription profile analysis of 14 laccase genes (Lelcc) under the conditions of different carbon sources, temperatures, and photoperiods. All laccase genes were significantly regulated by varying carbon source materials. The expression of only two laccase genes (Lelcc5 and Lelcc6) was induced by sodium-lignosulphonate and the expression of most laccase genes was specifically upregulated in glucose medium. Under different temperature conditions, the expression levels of most laccase genes decreased at 39 °C and transcription was significantly increased for Lelcc1, Lelcc4, Lelcc5, Lelcc9, Lelcc12, Lelcc13, and Lelcc14 after induction for 24 h at 10 °C, indicating their involvement in primordium differentiation. Tyrosinase, which is involved in melanin synthesis, was clustered with the same group as Lelcc4 and Lelcc7 in all the different photoperiod treatments. Meanwhile, five laccase genes (Lelcc8, Lelcc9, Lelcc12, Lelcc13, and Lelcc14) showed similar expression profiles to that of two blue light receptor genes (LephrA and LephrB) in the 12 h light/12 h dark treatment, suggesting the involvement of laccase genes in the adaptation process of L. edodes to the changing environment and fruiting-body formation. This study contributes to our understanding of the function of the different Lelcc genes and facilitates the screening of key genes from the laccase gene family for further functional research.

scholarly journals Transcriptional and Enzymatic Profiling of Pleurotus ostreatus Laccase Genes in Submerged and Solid-State Fermentation Cultures

2012 ◽  
Vol 78 (11) ◽  
pp. 4037-4045 ◽  
Author(s):  
Raúl Castanera ◽  
Gúmer Pérez ◽  
Alejandra Omarini ◽  
Manuel Alfaro ◽  
Antonio G. Pisabarro ◽  
...  
Keyword(s):  
Gene Family ◽  
Wheat Straw ◽  
Pleurotus Ostreatus ◽  
Submerged Fermentation ◽  
Expression Profiles ◽  
Water Soluble ◽  
White Rot ◽  
Accurate Estimation ◽  
Laccase Gene ◽  
Laccase Genes

ABSTRACTThe genome of the white rot basidiomycetePleurotus ostreatusincludes 12 phenol oxidase (laccase) genes. In this study, we examined their expression profiles in different fungal strains under different culture conditions (submerged and solid cultures) and in the presence of a wheat straw extract, which was used as an inducer of the laccase gene family. We used a reverse transcription-quantitative PCR (RT-qPCR)-based approach and focused on determining the reaction parameters (in particular, the reference gene set for the normalization and reaction efficiency determinations) used to achieve an accurate estimation of the relative gene expression values. The results suggested that (i) laccase gene transcription is upregulated in the induced submerged fermentation (iSmF) cultures but downregulated in the solid fermentation (SSF) cultures, (ii) theLacc2andLacc10genes are the main sources of laccase activity in the iSmF cultures upon induction with water-soluble wheat straw extracts, and (iii) an additional, as-yet-uncharacterized activity (Unk1) is specifically induced in SSF cultures that complements the activity of Lacc2 and Lacc10. Moreover, both the enzymatic laccase activities and theLaccgene family transcription profiles greatly differ between closely related strains. These differences can be targeted for biotechnological breeding programs for enzyme production in submerged fermentation reactors.

scholarly journals Molecular Cloning, Characterization, and Differential Expression of a Glucoamylase Gene from the Basidiomycetous Fungus Lentinula edodes

2000 ◽  
Vol 66 (6) ◽  
pp. 2531-2535 ◽  
Author(s):  
J. Zhao ◽  
Y. H. Chen ◽  
H. S. Kwan
Keyword(s):  
Gene Expression ◽  
Fruiting Body ◽  
Lentinula Edodes ◽  
Catalytic Domain ◽  
Coding Region ◽  
Fruiting Body Formation ◽  
Mycelium Growth ◽  
Body Formation ◽  
Basidiomycetous Fungus ◽  
Glucoamylase Gene

ABSTRACT The complete nucleotide sequence of putative glucoamylase genegla1 from the basidiomycetous fungus Lentinula edodes strain L54 is reported. The coding region of the genomic glucoamylase sequence, which is preceded by eukaryotic promoter elements CAAT and TATA, spans 2,076 bp. The gla1 gene sequence codes for a putative polypeptide of 571 amino acids and is interrupted by seven introns. The open reading frame sequence of thegla1 gene shows strong homology with those of other fungal glucoamylase genes and encodes a protein with an N-terminal catalytic domain and a C-terminal starch-binding domain. The similarity between the Gla1 protein and other fungal glucoamylases is from 45 to 61%, with the region of highest conservation found in catalytic domains and starch-binding domains. We compared the kinetics of glucoamylase activity and levels of gene expression in L. edodes strain L54 grown on different carbon sources (glucose, starch, cellulose, and potato extract) and in various developmental stages (mycelium growth, primordium appearance, and fruiting body formation). Quantitative reverse transcription PCR utilizing pairs of primers specific forgla1 gene expression shows that expression ofgla1 was induced by starch and increased during the process of fruiting body formation, which indicates that glucoamylases may play an important role in the morphogenesis of the basidiomycetous fungus.

scholarly journals Cloning and functional analysis of a laccase gene during fruiting body formation in Hypsizygus marmoreus

2015 ◽  
Vol 179 ◽  
pp. 54-63 ◽  
Author(s):  
Jinjing Zhang ◽  
Hui Chen ◽  
Mingjie Chen ◽  
Ang Ren ◽  
Jianchun Huang ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Fruiting Body ◽  
Laccase Gene ◽  
Fruiting Body Formation ◽  
Hypsizygus Marmoreus ◽  
Body Formation

scholarly journals Mapping of genes abundantly expressed during fruiting body formation of Lentinula edodes

2010 ◽  
Vol 60 (1) ◽  
pp. 81-86 ◽  
Author(s):  
Kazuhiro Miyazaki ◽  
Miho Sakai ◽  
Yasumasa Miyazaki
Keyword(s):  
Fruiting Body ◽  
Lentinula Edodes ◽  
Fruiting Body Formation ◽  
Body Formation

scholarly journals Draft Genome Sequence of the Fruiting Myxobacterium Nannocystis exedens DSM 71

2017 ◽  
Vol 5 (43) ◽  
Author(s):  
Anke Treuner-Lange ◽  
Marc Bruckskotten ◽  
Oliver Rupp ◽  
Alexander Goesmann ◽  
Lotte Søgaard-Andersen
Keyword(s):  
Genome Sequence ◽  
Fruiting Body ◽  
Draft Genome ◽  
Gc Content ◽  
Genetic Program ◽  
Draft Genome Sequence ◽  
Fruiting Bodies ◽  
Fruiting Body Formation ◽  
Content Type ◽  
Body Formation

ABSTRACT In response to starvation, members of the order Myxococcales form morphologically very different fruiting bodies. To determine whether fruiting myxobacteria share a common genetic program that leads to fruiting body formation, we sequenced and assembled the genome of Nannocystis exedens DSM 71 as two contigs with a total GC content of 72%.

Sign in / Sign up

Export Citation Format

Share Document