scholarly journals FT-IR Examination of the Development of Secondary Cell Wall in Cotton Fibers

Fibers ◽  
2015 ◽  
Vol 3 (4) ◽  
pp. 30-40 ◽  
Author(s):  
Michael Cintrón ◽  
Doug Hinchliffe
Keyword(s):  
Cell Wall ◽  
Secondary Cell Wall ◽  
Cotton Fibers ◽  
Ft Ir

Comparative Investigation of Secondary Cell Wall Development in Cotton Fiber Near Isogenic Lines Using Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR FT-IR)

2019 ◽  
Vol 73 (3) ◽  
pp. 329-336 ◽  
Author(s):  
Yongliang Liu ◽  
Hee-Jin Kim
Keyword(s):  
Cell Wall ◽  
Fourier Transform ◽  
Secondary Cell Wall ◽  
Fiber Strength ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Near Isogenic Lines ◽  
Significant Difference ◽  
Ft Ir ◽  
Fiber Maturity

In this investigation, we applied previously proposed simple algorithms to analyze the attenuated total reflection Fourier transform infrared (ATR FT-IR) spectra of cotton fibers during secondary cell wall (SCW) biosynthesis. The infrared crystallinity ( CIIR) and maturity ( MIR) indices were compared from developmental fibers representing two pairs of upland cotton near isogenic lines (NILs). One pair of NILs consisted of Texas Marker-1 (TM-1) and an immature fiber ( im) mutant that differ in fiber maturity. The other pair of NILs included MD52ne and MD90ne that show variations in fiber strength. The observations revealed significant difference in the MIR values between developmental TM-1 and im NILs grown at a field in crop year 2015, and also a significant difference in the CIIR values between these NILs grown at the same field in crop year 2011. These different patterns of CIIR and MIR values during fiber development for the two different crop years indicated the impact of genetics and crop year on the development of fiber maturity and crystallinity of the TM-1 and im fibers. Furthermore, the tendency of linking CIIR with MIR values suggested that the im fibers have more CIIR development than the TM-1 fibers when the fibers have the similar MIR values. In contrast, the NIL pair having variations in fiber strength showed insignificant differences in the patterns of CIIR and MIR as well as the relationship between CIIR and MIR values. The results suggested that CIIR and MIR indices from ATR FT-IR measurement could be used to facilitate the understanding of how fiber genetics and crop year affect fiber maturity and crystallinity during SCW biosynthesis.

Strength and Crystalline Structure of Developing Acala Cotton

1997 ◽  
Vol 67 (7) ◽  
pp. 529-536 ◽  
Author(s):  
Y.-L. Hsieh ◽  
X.-P. Hu ◽  
A. Nguyen
Keyword(s):  
Cell Wall ◽  
Secondary Cell Wall ◽  
Fiber Development ◽  
Single Fiber ◽  
Cotton Fibers ◽  
Cellulose Biosynthesis ◽  
Cellulose I ◽  
Peak Intensity ◽  
Crystallite Sizes ◽  
Fiber Breaking

Single fiber strengths and crystalline structures of greenhouse-grown Maxxa Acala cotton fibers at varying stages of development and at maturity are reported and compared with those of SJ-2 cotton fibers. Single fiber breaking forces of the Maxxa variety increase most significantly during the fourth week of fiber development; these increases correlate to the 60 to 90 mg seed fiber weight range. The forces required to break single fibers are similar for the SJ-2 and Maxxa varieties through the end of the fourth week of development. Beyond 30 dpa, both single fiber breaking forces and tenacities of the Maxxa cotton fibers are higher than those of the SJ-2 cotton fibers. Four waxd peaks located near 2θ angles of 14.7, 16.6, 22.7, and 34.4° are characteristic of the 101, 101, 002, and 040 reflections of cellulose I, respectively. The 002 peak intensity increases greatly during cellulose biosynthesis, indicating improved alignment of the glucosidic rings and improved order of atoms located within the glucosidic rings as the secondary cell wall thickens. The overall crystallinity and apparent crystallite sizes normal to the 101, 101, and 002 planes increase with fiber development for both varieties. Within each variety, the single fiber breaking forces are positively related to both the overall crystallinity and crystallite sizes. Between these two varieties, increasing breaking forces and tenacities appear to be related more to crystallite size than to crystallinity.

scholarly journals Kinetics of Cellulose Deposition in Developing Cotton Fibers Studied by Thermogravimetric Analysis

Fibers ◽  
2019 ◽  
Vol 7 (9) ◽  
pp. 78 ◽  
Author(s):  
Luis Cabrales ◽  
Noureddine Abidi
Keyword(s):  
Cell Wall ◽  
Thermogravimetric Analysis ◽  
Secondary Cell Wall ◽  
Structural Changes ◽  
Developmental Stages ◽  
Analytical Techniques ◽  
Cotton Fibers ◽  
Cellulose Deposition ◽  
Cotton Genotypes ◽  
Cell Wall Development

During cotton fibers development, important structural changes occur, which lead to cellulose deposition and organization in the secondary cell wall. Several studies have focused on the analysis of the cell wall extracts of cotton fibers to gain an understanding of the changes in carbohydrate profiles and to determine the changes in crystallinity, cellulosic and non-cellulosic compounds at various stages of the fiber cell wall development. In this research, thermogravimetric analysis (TGA) was used to study intact fibers harvested from two cotton genotypes. Cellulose macromolecules structural changes occurring during different developmental stages were studied. The results from TGA technique were in agreement with results from other analytical techniques, which indicates that TGA could be a great tool to investigate the onset of cellulose deposition and to evaluate the cell wall composition during fiber development. The results obtained in this study demonstrated that the initiation of the secondary cell wall is genotype-dependent.

scholarly journals Auxin Directly Upregulates GhRAC13 Expression to Promote the Onset of Secondary Cell Wall Deposition in Cotton Fibers

2020 ◽  
Vol 11 ◽  
Author(s):  
Mi Zhang ◽  
Huizhen Cao ◽  
Jing Xi ◽  
Jianyan Zeng ◽  
Juan Huang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Secondary Cell Wall ◽  
Cotton Fibers ◽  
Wall Deposition ◽  
Cell Wall Deposition

Chemical imaging of secondary cell wall development in cotton fibers using a mid-infrared focal-plane array detector

2016 ◽  
Vol 87 (9) ◽  
pp. 1040-1051 ◽  
Author(s):  
Michael Santiago Cintrón ◽  
Chanel Fortier ◽  
Doug J Hinchliffe ◽  
James E Rodgers
Keyword(s):  
Cell Wall ◽  
Secondary Cell Wall ◽  
Chemical Imaging ◽  
Fiber Development ◽  
Array Detector ◽  
Intensity Increase ◽  
Ir Microscopy ◽  
Fiber Properties ◽  
Cell Wall Development ◽  
Ft Ir

Market demands for cotton varieties with improved fiber properties also call for the development of fast, reliable analytical methods for monitoring fiber development and measuring their properties. Currently, cotton breeders rely on instrumentation that can require significant amounts of sample, which complicates fiber development studies. Herein, we explored the use of high-resolution, Fourier-transform infrared (FT-IR) microscopy to examine cotton fiber secondary cell wall development in single fibers. Notably, there was a marked intensity increase for the C-O bending region near 1015 cm–1 and the C-H stretch at 2900 cm–1. These changes agree with those observed with macroscopic FT-IR tests. Chemical distribution maps and principal component analysis plots visually depict these spectral changes. Our results suggest the FT-IR microscopy can potentially be utilized as a tool to monitor and assess important fiber properties, such as cotton maturity, during fiber development.

scholarly journals Gibberellin Overproduction Promotes Sucrose Synthase Expression and Secondary Cell Wall Deposition in Cotton Fibers

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e96537 ◽  
Author(s):  
Wen-Qin Bai ◽  
Yue-Hua Xiao ◽  
Juan Zhao ◽  
Shui-Qing Song ◽  
Lin Hu ◽  
...  
Keyword(s):  
Cell Wall ◽  
Sucrose Synthase ◽  
Secondary Cell Wall ◽  
Cotton Fibers ◽  
Wall Deposition ◽  
Cell Wall Deposition

Brassinosteroid signaling affects secondary cell wall deposition in cotton fibers

2015 ◽  
Vol 65 ◽  
pp. 334-342 ◽  
Author(s):  
Yan Sun ◽  
Suresh Veerabomma ◽  
Mohamed Fokar ◽  
Noureddine Abidi ◽  
Eric Hequet ◽  
...  
Keyword(s):  
Cell Wall ◽  
Secondary Cell Wall ◽  
Cotton Fibers ◽  
Wall Deposition ◽  
Brassinosteroid Signaling ◽  
Cell Wall Deposition
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