scholarly journals Production, Characterization, and Industrial Application of Pectinase Enzyme Isolated from Fungal Strains

Fermentation ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 59 ◽  
Author(s):  
Sudeep KC ◽  
Jitendra Upadhyaya ◽  
Dev Raj Joshi ◽  
Binod Lekhak ◽  
Dhiraj Kumar Chaudhary ◽  
...  
Keyword(s):  
Substrate Concentration ◽  
Agar Medium ◽  
Soil Samples ◽  
Food Industries ◽  
Fungal Strains ◽  
Pectinolytic Activity ◽  
Soluble Solid ◽  
Pectinase Enzyme ◽  
Pectinase Production ◽  
Potential Use

Pectinases are the group of enzymes that catalyze the degradation of pectic substances. It has wide applications in food industries for the production and clarification of wines and juices. The aim of this study was to isolate, screen and characterize pectinase from fungi isolated from various soil samples and evaluate its application in juice clarification. Fungal strains were isolated and screened primarily using 1% citruspectin incorporated potato dextrose agar (PDA) and secondarily using pectinase screening agar medium (PSAM) for pectinolytic organisms. The enzyme was produced by submerged state fermentation and assayed using the dinitro salicylic acid (DNS) method. From 20 different soil samples, 55 fungal isolates were screened primarily and, among them, only 14 isolates were subjected for secondary screening. Out of 14, only four strains showed the highest pectinolytic activity. Among four strains, Aspergillus spp. Gm showed the highest enzyme production at a 48-h incubation period, 1% substrate concentration, and 30 °C temperature. The thermal stability assessment resulted that the activity of pectinase enzyme declines by 50% within 10 min of heating at 60 °C. The optimum temperature, pH, and substrate concentration for the activity of enzyme was 30 °C (75.4 U/mL), 5.8 (72.3 U/mL), and 0.5% (112.0 U/mL), respectively. Furthermore, the yield of the orange juice, the total soluble solid (TSS), and clarity (% transmittance) was increased as the concentration of the pectinase increased, indicating its potential use in juice processing. Overall, the strain Aspergillus spp. Gm was identified as a potent strain for pectinase production in commercial scale.

scholarly journals Optimization of Cultural Conditions for the Production of Pectinase from Selected Fungal Strain

2014 ◽  
Vol 8 ◽  
pp. 65-70 ◽  
Author(s):  
Rajiv Dhital ◽  
Om Prakash Panta ◽  
Tika Bahadur Karki
Keyword(s):  
Culture Medium ◽  
Soil Samples ◽  
Fungal Strain ◽  
Cultural Conditions ◽  
Pectinolytic Activity ◽  
Soluble Solid ◽  
Pectinase Production ◽  
Enzyme Application ◽  
Selection Of ◽  
Pectin Substances

Pectinase are the group of enzymes that catalyze the degradation of pectin substances through de-polymerisation and de-esterification. This study is concerned on the isolation, screening and selection of suitable strain of pectinolytic organism and optimization of cultural conditions for the biosynthesis of pectinase. From the soil samples collected from Lalitpur, Kathmandu, Gulmi, Manang and Dang, 18 fungal colonies were isolated on the basis of halozone formation on Potato dextrose agar and identified. Enzyme production was carried out by submerged state fermentation. The partially purified enzymes showing higher pectinolytic activity were characterized on the basis of temperature of incubation, substrate concentration and pH of the substrate by Dinitro salicylic acid assay (DNSA) method. The fungal isolate showing highest enzyme activity was subjected to optimization of culture medium for the production of enzymes. On optimization, it was found that MG1 (Aspergillus niger) was the most potent strain at 1.5% substrate (pectin) concentration, pH 4.5 and temperature of 30°C. On the enzyme application, the yield of the orange juice, Total Soluble Solid and absorbance increased as the concentration of the enzyme increased and hence increasing the possibility of being used commercially for maximum pectinase production. DOI: http://dx.doi.org/10.3126/jfstn.v8i0.11752 J. Food Sci. Technol. Nepal, Vol. 8 (65-70), 2013

scholarly journals Pectinase production by Aspergillus niger isolated from decomposed apple skin

2013 ◽  
Vol 48 (1) ◽  
pp. 25-32 ◽  
Author(s):  
S Islam ◽  
B Feroza ◽  
AKMR Alam ◽  
S Begum
Keyword(s):  
Aspergillus Niger ◽  
Incubation Temperature ◽  
Nitrogen Sources ◽  
Inoculum Size ◽  
Point Of View ◽  
Media Composition ◽  
Maximal Level ◽  
Pectinolytic Activity ◽  
Pectinase Activity ◽  
Pectinase Production

Pectinase activity among twelve different fungal strains, Aspergillus niger IM09 was identified as a potential one to produce maximal level 831 U/g at pH 4.0. Media composition, incubation temperature, incubation time, substrate concentration, aeration, inoculum size, assay temperature and nitrogen sources were found to effect pectinase activity. Moisture content did not affect the activity significantly. Media composition was varied to optimize the enzyme production in solid state fermentation. It was observed that the highest pectinase activity of 831.0 U/g was found to produce in presence of yeast extract as a nitrogen source in combination with ammonium sulfate in assay media. Aeration showed positive significant effects on pectinase production 755 U/g at 1000 ml flasks. The highest pectinase production was found at 2 g pectin (521 U/g) used as a substrate. Pectinolytic activity was found to have undergone catabolite repression with higher pectin concentration (205 U/g at 5 g pectin). The incubation period to achieve maximum pectinase activity by the isolated strain Aspergillus niger IM09 was 3 days, which is suitable from the commercial point of view. DOI: http://dx.doi.org/10.3329/bjsir.v48i1.15410 Bangladesh J. Sci. Ind. Res. 48(1), 25-32, 2013

scholarly journals Pectinase production by fungal strains in solid-state fermentation using agro-industrial bioproduct

2004 ◽  
Vol 47 (5) ◽  
pp. 813-819 ◽  
Author(s):  
Natalia Martin ◽  
Simone Regina de Souza ◽  
Roberto da Silva ◽  
Eleni Gomes
Keyword(s):  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Sugar Cane Bagasse ◽  
Pectin Lyase ◽  
Optimum Activity ◽  
Fungal Strains ◽  
Penicillium Sp ◽  
Polygalacturonase Production ◽  
Pectinase Production

Pectin lyase and polygalacturonase production by newly isolated fungal strains was carried out in solid-state fermentation. Moniliella SB9 and Penicillium sp EGC5 produced polygalcturonase (PG) and pectin lyase (PL) on mixture of orange bagasse, sugar cane bagasse and wheat bran as substrate. PG and PL produced by Moniliella presented optimum activity at pH 4.5 and 10.0 and at 55 and 45°C, respectively, while these enzymes from Penicillium sp presented optimum activity at pH 4.5-5.0 and 9.0 and 40°C, respectively.

scholarly journals Potential use of a chemical leaching reject from a kaolin industry as agricultural fertilizer

2007 ◽  
Vol 31 (5) ◽  
pp. 939-946
Author(s):  
Fabiana Rodrigues Ribeiro ◽  
Fernando Barboza Egreja Filho ◽  
José Domingos Fabris ◽  
Wagner da Nova Mussel ◽  
Roberto Ferreira Novais
Keyword(s):  
Paper Industry ◽  
Soil Samples ◽  
Limiting Factor ◽  
Metallic Zinc ◽  
Chemical Leaching ◽  
Industrial Processing ◽  
Industrial Residue ◽  
Potential Use ◽  
Agricultural Fertilizer ◽  
Soil Fertilizer

The industrial refining of kaolin involves the removal of iron oxides and hydroxides along with other impurities that cause discoloration of the final product and depreciate its commercial value, particularly undesirable if destined to the paper industry. The chemical leaching in the industrial processing requires treatments with sodium hyposulfite, metallic zinc, or sulfuric and phosphoric acids, in order to reduce, dissolve and remove ferruginous compounds. To mitigate the environmental impact, the acidic effluent from the leaching process must be neutralized, usually with calcium oxide. The resulting solid residue contains phosphorous, zinc, and calcium, among other essential nutrients for plant growth, suggesting its use as a macro and micronutrient source. Samples of such a solid industrial residue were used here to evaluate their potential as soil fertilizer in an incubation greenhouse experiment with two soil samples (clayey and medium-textured). The small pH shift generated by applying the residue to the soil was not a limiting factor for its use in agriculture. The evolution of the concentrations of exchangeable calcium, and phosphorous and zinc extractability by Mehlich-1 extractant during the incubation period confirms the potential use of this industrial residue as agricultural fertilizer.

scholarly journals The influence of silica functionalized with silanes on migration of heavy metals in soil

2016 ◽  
Vol 18 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Piotr Grzesiak ◽  
Joanna Łukaszyk ◽  
Elżbieta Gabała ◽  
Joanna Kurczewska ◽  
Grzegorz Schroeder
Keyword(s):  
Heavy Metals ◽  
Copper Smelter ◽  
Soil Samples ◽  
Functionalized Silica ◽  
Impact Zone ◽  
Unmodified Silica ◽  
Metal Migration ◽  
The Impact ◽  
Potential Use ◽  
Heavy Metals And Arsenic

Abstract 3-Mercaptopropyl-trimethoxysilane and [3-(2-aminoethylamino)propyl]trimethoxysilane were used to functionalize the surface of silica from Piotrowice in Poland to stabilize heavy metals (HMs) and arsenic in soil. The soil for the study was sampled from the impact zone of Głogów Copper Smelter and Refinery. The soil samples were exposed to five-step Tessier sequential extraction. The speciation studies were limited to five sequentially defined fractions in which metal content was determined. The addition of unmodified silica did not affect significantly the concentration of metals in individual fractions. Significant changes were noted upon introduction of functionalized silica in the soil. The hybrid formulations obtained significantly reduce the release of heavy metals and arsenic from soil sorption complex. The results indicate the potential use of functional formulations for reduction of metal migration in soil in the areas of exceeded concentration of heavy metals and arsenic in the soil, caused by industrial activity.

Potential use of native fungal strains for assisted uranium retention

2015 ◽  
Vol 81 ◽  
pp. 173-178 ◽  
Author(s):  
Anna Grandin ◽  
Anna Ogar ◽  
Viktor Sjöberg ◽  
Bert Allard ◽  
Stefan Karlsson
Keyword(s):  
Fungal Strains ◽  
Potential Use

scholarly journals Assessment of Antioxidant and Antibacterial Activities of Cystoseira Mediterranea and Padina Pavonica from Algerian North-East for A Potential Use as a Food Preservative

2020 ◽  
Vol 49 (4) ◽  
pp. 498-504
Author(s):  
B. Keramane ◽  
N. Touati ◽  
K. Saidani ◽  
A. Taguelmimt ◽  
I. Zair ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Antibacterial Activities ◽  
Food Preservative ◽  
Food Industries ◽  
E Coli ◽  
North East ◽  
Staphyloccocus Aureus ◽  
Activity Screening ◽  
Extraction Solvents ◽  
Potential Use

The aim of this work was to evaluate the antioxidant and antibacterial activities of two marine algae, Cystoseira mediterranea and Padina pavonica, extracts. Total phenols (TPC), carotenoids, and phlorotannins contents of the extracts obtained by four extraction solvents were determined and compared. The highest TPC content was observed for aqueous extract of C. mediterranea with 37.09±0.46 mg GAE/g DE followed by ethanol extract of P. pavonica (24.28 ±0.99 mg GAE/g DE), which showed the highest phlorotannins content (1.18±0.18 mg PE/g DE), while its methanol extract held carotenoids content of 66.96 ±4.78 μg g–1 DE. Ethanol extract of C. mediterranea exhibited the best antioxidant activity with an EC50 of 58.3 ±1.16 μg ml . The antibacterial activity screening against MRSA and E. coli showed that ethanol extract of C. mediterranea towards a Methicillin resistant Staphyloccocus aureus (20.33±0.28 mm) and E. coli (15.66±0.57 mm) was more efficient with MICs about 80 mg ml–1 and 20 mg ml–1, respectively. Ethanol extract of C. mediterranea seems to have the highest potential for use in food industries.

scholarly journals Bio-remedial Effect of Specific Micro-organisms (Bacteria) on Used Engine Oil: A Case Study of Some Mechanic Workshops in Maiduguri Metropolitan Council (MMC)

2020 ◽  
Vol 1 (3) ◽  
Author(s):  
Ibrahim Yerima ◽  
G. A. Felix ◽  
M. I. Ahmad
Keyword(s):  
Zea Mays ◽  
Agar Medium ◽  
Bacterial Species ◽  
Soil Samples ◽  
Engine Oil ◽  
Bacillus Sp ◽  
Used Engine Oil ◽  
Micro Organisms ◽  
Effective Growth

The potential of three micro-organisms (Pseudomonas, Streptococcus and Bacillus sp) were isolated from hydrocarbon contaminated soil and were evaluated for their biodegradation ability. The rate of biodegradation of the engine oil in the soil samples were exposed to used engine oil with different exposure rates of 5,10,15 and 20 years  were studied for a period of three (3) weeks under greenhouse experiment. The soil samples were obtained from four different mechanic workshops in M.M.C and they were plated on nutrients agar and oil agar medium to isolate the bacterial species from the spilled soil samples. All the micro-organisms used in this study showed their abilities to remediate soil exposed to used engine oil and the remediated soil samples were able to support the growth of Maize ( Zea mays) after 10 years  effective growth

scholarly journals Fungi associated with free-living soil nematodes in Turkey

2015 ◽  
Vol 67 (4) ◽  
pp. 1173-1183
Author(s):  
Salih Karabörklü ◽  
Abdurrahman Ayvaz ◽  
Semih Yilmaz ◽  
Ugur Azizoglu
Keyword(s):  
Phylogenetic Trees ◽  
World Wide ◽  
Large Subunit ◽  
Soil Samples ◽  
Soil Types ◽  
Soil Nematodes ◽  
Free Living ◽  
Fungal Strains

Free-living soil nematodes have successfully adapted world-wide to nearly all soil types from the highest to the lowest of elevations. In the current study, nematodes were isolated from soil samples and fungi associated with these free-living soil nematodes were determined. Large subunit (LSU) rDNAs of nematode-associated fungi were amplified and sequenced to construct phylogenetic trees. Nematode-associated fungi were observed in six nematode strains belonging to Acrobeloides, Steinernema and Cephalobus genera in different habitats. Malassezia and Cladosporium fungal strains indicated an association with Acrobeloides and Cephalobus nematodes, while Alternaria strains demonstrated an association with the Steinernema strain. Interactions between fungi and free-living nematodes in soil are discussed. We suggest that nematodes act as vectors for fungi.

scholarly journals Comparison of Biological Activity of Field Isolates of Steinernema feltiae with a Commercial S. feltiae Biopesticide Product

Insects ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 816
Author(s):  
Joanna Matuska-Łyżwa ◽  
Paulina Żarnowiec ◽  
Wiesław Kaca
Keyword(s):  
Entomopathogenic Nematodes ◽  
First Generation ◽  
Biological Activities ◽  
Soil Samples ◽  
Steinernema Feltiae ◽  
Adult Males ◽  
Field Isolates ◽  
Insect Trap ◽  
The Family ◽  
Potential Use

Insect trap studies were carried out to determine the presence of entomopathogenic nematodes (EPN) from the family Steinernematidae in the soils of Poland and to compare the biological activities of field nematode isolates with nematodes from commercial biopesticide. The fauna of these organisms in central Poland is poorly studied in both taxonomic and biological terms. Tilled soils representative of this region were sampled from cultivated fields. EPN were isolated from soil samples under laboratory conditions and identified using a key for species identification and molecular analysis. Basic morphometric parameters of infective juveniles and adult males of the first generation were determined. The research showed that males and infective juveniles Steinernema feltiae from Łoniów were the largest. The smallest infective juveniles were found in the isolate from Oblasy, and the smallest males in the isolate from Danków. In Poland, new field isolates showed close genetic similarity to other S. feltiae isolates. The research showed that the field isolates from Poland had greater infectivity and rate of reproduction compared with nematodes from the commercial biopesticide. The findings indicate the potential use of field S. feltiae isolates from Poland (iso1Lon, iso1Dan and iso1Obl) to develop new biopesticide products.

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