scholarly journals Capnophilic Lactic Fermentation from Thermotoga neapolitana: A Resourceful Pathway to Obtain Almost Enantiopure L-lactic Acid

Fermentation ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 34 ◽  
Author(s):  
Genoveffa Nuzzo ◽  
Simone Landi ◽  
Nunzia Esercizio ◽  
Emiliano Manzo ◽  
Angelo Fontana ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Enantiomeric Excess ◽  
Thermophilic Bacterium ◽  
Racemic Mixture ◽  
Thermotoga Neapolitana ◽  
High Concentration ◽  
Lactic Fermentation ◽  
Bacterial Fermentation ◽  
First Time

The industrial production of lactic acid (LA) is mainly based on bacterial fermentation. This process can result in enantiopure or racemic mixture according to the producing organism. Between the enantiomers, L-lactic acid shows superior market value. Recently, we reported a novel anaplerotic pathway called capnophilic lactic fermentation (CLF) that produces a high concentration of LA by fermentation of sugar in the anaerobic thermophilic bacterium Thermotoga neapolitana. The aim of this work was the identification of the enantiomeric characterization of the LA produced by T. neapolitana and identification of the lactate dehydrogenase in T. neapolitana (TnLDH) and related bacteria of the order Thermotogales. Chemical derivatization and GC/MS analysis were applied to define the stereochemistry of LA from T. neapolitana. A bioinformatics study on TnLDH was carried out for the characterization of the enzyme. Chemical analysis showed a 95.2% enantiomeric excess of L-LA produced by T. neapolitana. A phylogenetic approach clearly clustered the TnLDH together with the L-LDH from lactic acid bacteria. We report for the first time that T. neapolitana is able to produce almost enantiopure L-lactic acid. The result was confirmed by bioinformatics analysis on TnLDH, which is a member of the L-LDH sub-family.

scholarly journals Neutralizing Effects of Nectandra angustifolia Extracts against Bothrops neuwiedi Snake Venom

2011 ◽  
Vol 6 (9) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Ana M. Torres ◽  
Francisco J. Camargo ◽  
Gabriela A. L. Ricciardi ◽  
Armando I. A. Ricciardi ◽  
Eduardo Dellacassa
Keyword(s):  
Essential Oil ◽  
Snake Venom ◽  
Enantiomeric Excess ◽  
Ethanol Extract ◽  
Active Constituents ◽  
Neutralizing Capacity ◽  
Coagulant Activity ◽  
First Time ◽  
Bothrops Neuwiedi

Leaves extracts and essential oil of Nectandra angustifolia were explored for the first time for neutralization of Bothrops neuwiedi diporus snake venom. The ethanol extract was the most active and inhibited both venom activities (hemolytic and coagulant), while the oil was only active on the coagulant activity. These observations confirmed that certain medicinal plants from Corrientes and Chaco Provinces possess significant snake venom neutralizing capacity and need further examination for their active constituents. Analysis by GC and GC-MS of the essential oil and the enantiomeric excess found for α-pinene, β-pinene and limonene allowed a better characterization of this species.

Kinetic modeling of hydrogen and L-lactic acid production by Thermotoga neapolitana via capnophilic lactic fermentation of starch

2021 ◽  
Vol 332 ◽  
pp. 125127
Author(s):  
Nirakar Pradhan ◽  
Giuliana d'Ippolito ◽  
Laura Dipasquale ◽  
Giovanni Esposito ◽  
Antonio Panico ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Kinetic Modeling ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Thermotoga Neapolitana ◽  
Lactic Fermentation

scholarly journals Bioremediation of Cadmium Contaminated Effluent by Sporosarcina luteola: A Bacterium Isolated from Soil near Wazirpur Industrial Area, New Delhi, India

2019 ◽  
Vol 31 (11) ◽  
pp. 2642-2646
Author(s):  
R. Gupta ◽  
T. Kumar ◽  
A. Mittal
Keyword(s):  
Chemical Characterization ◽  
Industrial Area ◽  
New Delhi ◽  
High Concentration ◽  
Phylogeny Analysis ◽  
Physico Chemical ◽  
Iron Manganese ◽  
First Time ◽  
Metal Tolerant Bacteria

Heavy metals pollution is emerging as a threat to ecological systems causing various problems to mankind, plants and animals. Aim of the present study was to isolate and identify cadmium tolerant bacteria from the soil of Wazirpur industrial area of New Delhi (India). The study involved physico-chemical characterization of the polluted soil which was found to contain high concentration of iron, manganese and cadmium at 352, 15.3 g/kg soil and 3.16 ppm, respectively. One bacterial strain was identified as Sporosarcina luteola on the basis of morphological, biochemical and phylogeny analysis. Strain Sporosarcina luteola was highly resistant to Cd up to 5mM (mM= millimolar) when cultured in solidified nutrient agar plates and 7.2 mM in nutrient broth. Sporosarcina luteola has also showed substantial growth in presence of Co, Pb, Fe and Mn upto 2.0, 2.0, 3.5 and 4.0 mM, respectively in liquid medium. Optimum growth of identified bacteria was shown at 37 ºC, 7.0 pH and it tolerated up to 3 % sodium chloride (w/v). This is reported for the first time that Sporosarcina luteola (metal-tolerant bacteria) has potential of removal of cadmium from industrially contaminated soil.

scholarly journals Characterization of Indigenous Lactic Acid Bacteria in Cow Milk of the Maltese Islands: A Geographical and Seasonal Assessment

2020 ◽  
Vol 8 (6) ◽  
pp. 812
Author(s):  
Elisa Garroni ◽  
Agapi I. Doulgeraki ◽  
Foteini Pavli ◽  
David Spiteri ◽  
Vasilis P. Valdramidis
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Geographical Distribution ◽  
Lactobacillus Casei ◽  
Lactobacillus Rhamnosus ◽  
Cow Milk ◽  
Eastern Region ◽  
First Time ◽  
Lactobacillus Spp

A geographical and seasonal assessment of indigenous lactic acid bacteria (LAB) in Maltese cow milk was conducted in this study. To investigate this, milk was collected from different regions of Malta during winter and summer seasons. Total viable counts (TVC) and LAB population were enumerated. Afterwards, LAB were isolated and identified by molecular methods. According to the results, similar TVC were enumerated on winter and summer samples, while highest LAB population was detected on summer samples. LAB isolates were grouped in seven different clusters which were assigned to Lactobacillus casei, Pediococcus pentosaceus, Lactobacillus plantarum, Weissella paramesenteroides, Lactobacillus rhamnosus, Lactococcus lactis, and Lactococcus garvieae. In addition, Enterococcus and Streptococcus species were also isolated. Season seemed to affect the genus/species of LAB since Lactobacillus were mainly isolated from winter samples, while Lactococcus and Enterococcus species were the main genera identified in summer samples. Regarding the geographical distribution, the majority of the Lactobacillus spp. were isolated from the South-eastern region in both seasons. In conclusion, through this study the diversity of indigenous LAB in the Maltese cow milk was monitored for the first time and highlighted that the microbial communities are affected by seasonality and geographical distribution of the farms.

scholarly journals Simultaneous synthesis of lactic acid and hydrogen from sugars via capnophilic lactic fermentation by Thermotoga neapolitana cf capnolactica

2019 ◽  
Vol 125 ◽  
pp. 17-22 ◽  
Author(s):  
Nirakar Pradhan ◽  
Giuliana d’Ippolito ◽  
Laura Dipasquale ◽  
Giovanni Esposito ◽  
Antonio Panico ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Thermotoga Neapolitana ◽  
Lactic Fermentation ◽  
Simultaneous Synthesis

TBE in Sweden

2020 ◽  
Keyword(s):  
Genetic Characterization ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Second Phase ◽  
Serum Samples ◽  
Tick Borne Encephalitis ◽  
Specific Immunoglobulin ◽  
Tick Borne Encephalitis Virus ◽  
First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

Management and engineering solutions to improve stripping equipment efficiency in Chernogorksy open pit mine

2018 ◽  
pp. 36-48
Author(s):  
G. N. Shapovalenko ◽  
S. N. Radionov ◽  
V. V. Gorbunov ◽  
V. A. Khazhiev ◽  
V. Yu. Zalyadnov ◽  
...  
Keyword(s):  
Open Pit Mine ◽  
Open Pit ◽  
High Concentration ◽  
Open Pit Mines ◽  
Blasting Operation ◽  
Internal Dump ◽  
Mining Conditions ◽  
Mining Front ◽  
Comparison Of Equipment

Chernogosky open pit mine integrates truck-and-shovel system of mining with overburden rehandling to internal dump with a set of walking excavators for rehandling of overburden to mined-out area of the pit. It is possible to improve efficiency of stripping in the conditions of Chernogorsky OPM by reducing percentage of stripping with more expensive handling system. The relevant research and solutions to this effect are presented in this article. Comparative characterization of mining conditions and parameters of mining systems applied is given for open pit mines Chernogorsky, Turnui, Nazarovsky, Vostochno-Beisky and Izykh. The comparative analysis points at the need to account for difficulty of mining and process sites in comparison of equipment productivity. High concentration of mining machines, which is conditioned by narrow mining front and simultaneous operation of five faces, as well as blasting operation implemented every 1-2 days, are recognized as the main constraints of excavator capacity in mining with direct dumping in Chernogorsky open pit mine. The management and engineering solutions implemented in the mine and resulted in higher efficiency of draglines are described.

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