scholarly journals Proteomic Analysis: Explosive Salt Accumulation in Leaves of Morus alba L. under Salt Stress

Forests ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1384
Author(s):  
Jiajun Yang ◽  
Yongbo Wu
Keyword(s):  
Salt Stress ◽  
Internal State ◽  
Redox Homeostasis ◽  
Sodium Content ◽  
Morus Alba ◽  
Label Free ◽  
Salt Accumulation ◽  
Ion Migration ◽  
Quantitative Analyses ◽  
Treatment Concentration

The salt tolerance of glycophytes is thought to be related to their ability to restrict sodium access to their aboveground parts. A previous study on the mulberry (Morus alba L.) revealed a phenomenon of explosive salt accumulation in the leaves after exceeding a certain treatment concentration. Here, we aim to observe the internal state of mulberry seedlings under salt stress by the proteomic method and to identify the possible inducements associated with salt bursts. In this study, the target treatments for TMT-label free quantitative analyses were determined by measuring the sodium content in the roots and leaves. The results showed that the expressions of proteins classified as “plant hormones”, “ion channels”, “REDOX homeostasis”, “cytoskeleton” and “cell wall” changed significantly after salt bursts. This phenotype is associated with the destruction of the apoplast, in which the assembly of the Casparian strip may be affected by the inhibition of some key proteins, indirectly increasing the rate of ion migration through the endodermis into the shoots.

scholarly journals Comparative Label-Free Quantitative Proteomics Analysis Reveals the Essential Roles of N-Glycans in Salt Tolerance by Modulating Protein Abundance in Arabidopsis

2021 ◽  
Vol 12 ◽  
Author(s):  
Chuanfa Liu ◽  
Guanting Niu ◽  
Xiaowen Li ◽  
Huchen Zhang ◽  
Huawei Chen ◽  
...  
Keyword(s):  
Salt Stress ◽  
Quantitative Proteomics ◽  
Regulatory Networks ◽  
Salt Sensitivity ◽  
Label Free ◽  
Salt Stress Response ◽  
Protein Levels ◽  
Substrate Preferences ◽  
Associated Proteins ◽  
Quantitative Analyses

Many pieces of evidence show that the adaptive response of plants to salt stress requires the maturation of N-glycan on associated proteins. However, it is still little known about the salt-responsive glycoproteins that function in this process. In the present study, we identified salt-responsive glycoproteins in wild-type (WT) Arabidopsis and two mutants defective in N-glycan maturation, mns1 mns2 and cgl1. A total of 97 proteins with abundance changes of >1.5‐ or <0.67-fold were identified against salt stress by label-free liquid chromatography coupled mass spectrometry (LC-MS/MS) quantitative analyses. A comparison of differentially abundant glycoproteins (DAGs) indicated the substrate preferences regulated by MNS1/MNS2 and CGL1. In addition, the DAGs in mns1 mns2 hardly form functional regulatory networks in STRING analysis. Comparably, the regulatory network in cgl1 was visible and shared overlapping with that in WT. Such difference may supply the evidence to partially explain the lower salt sensitivity of mutant cgl1 than mns1 mns2. We further confirmed that two N-glycosylation clients, peroxidases PRX32 and PRX34, were involved in the salt stress response since the double mutants showed enhanced salt sensitivity. Together, our study provided proteomic evidence that N-glycans are crucial for modulating stress-responsive protein levels, and several novel glycoproteins responsible for salt stress tolerance in Arabidopsis were listed. Data are available via ProteomeXchange with identifier PXD006893.

scholarly journals Phosphoproteomic Analysis of Two Contrasting Maize Inbred Lines Provides Insights into the Mechanism of Salt-Stress Tolerance

2019 ◽  
Vol 20 (8) ◽  
pp. 1886 ◽  
Author(s):  
Xiaoyun Zhao ◽  
Xue Bai ◽  
Caifu Jiang ◽  
Zhen Li
Keyword(s):  
Salt Stress ◽  
Inbred Line ◽  
Carbon Metabolism ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Glutathione Metabolism ◽  
Label Free ◽  
Plasma Membrane Intrinsic Protein ◽  
Sodium Hydrogen ◽  
Salt Response ◽  
Sodium Hydrogen Exchanger

Salinity is a major abiotic stress that limits maize yield and quality throughout the world. We investigated phosphoproteomics differences between a salt-tolerant inbred line (Zheng58) and a salt-sensitive inbred line (Chang7-2) in response to short-term salt stress using label-free quantitation. A total of 9448 unique phosphorylation sites from 4116 phosphoproteins in roots and shoots of Zheng58 and Chang7-2 were identified. A total of 209 and 243 differentially regulated phosphoproteins (DRPPs) in response to NaCl treatment were detected in roots and shoots, respectively. Functional analysis of these DRPPs showed that they were involved in carbon metabolism, glutathione metabolism, transport, and signal transduction. Among these phosphoproteins, the expression of 6-phosphogluconate dehydrogenase 2, pyruvate dehydrogenase, phosphoenolpyruvate carboxykinase, glutamate decarboxylase, glutamate synthase, l-gulonolactone oxidase-like, potassium channel AKT1, high-affinity potassium transporter, sodium/hydrogen exchanger, and calcium/proton exchanger CAX1-like protein were significantly regulated in roots, while phosphoenolpyruvate carboxylase 1, phosphoenolpyruvate carboxykinase, sodium/hydrogen exchanger, plasma membrane intrinsic protein 2, glutathione transferases, and abscisic acid-insensitive 5-like protein were significantly regulated in shoots. Zheng58 may activate carbon metabolism, glutathione and ascorbic acid metabolism, potassium and sodium transportation, and the accumulation of glutamate to enhance its salt tolerance. Our results help to elucidate the mechanisms of salt response in maize seedlings. They also provide a basis for further study of the mechanism underlying salt response and tolerance in maize and other crops.

scholarly journals Identification of Phosphorylated Cyclin-Dependent Kinase 1 Associated with Colorectal Cancer Survival Using Label-Free Quantitative Analyses

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0158844 ◽  
Author(s):  
Peng-Chan Lin ◽  
Yi-Fang Yang ◽  
Yu-Chang Tyan ◽  
Eric S. L. Hsiao ◽  
Po-Chen Chu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Survival ◽  
Cyclin Dependent Kinase ◽  
Label Free ◽  
Colorectal Cancer Survival ◽  
Quantitative Analyses

scholarly journals Variation in Salinity through the Soil Profile in South Coastal Region of Bangladesh

2018 ◽  
Vol 42 (1) ◽  
pp. 11-23
Author(s):  
Mohammad Asadul Haque
Keyword(s):  
Electrical Conductivity ◽  
Salt Stress ◽  
Soil Profile ◽  
Ph Value ◽  
Soil Depth ◽  
Coastal Region ◽  
Soil Samples ◽  
Salt Accumulation ◽  
Top Soil ◽  
Academy Of Sciences

The spatial variability of salt accumulation through the soil profile was studied at Latachapali union of Kalapara upazila, Patuakhali district, Bangladesh. The soil samples were collected from 30 locations covering six villages of the union: Kuakata, Malapara, Fashipara, Khajura, Mothaopara and Tajepara. Five locations were randomly selected from each village. From each location soil samples were collected from three soil depths at 0-2 cm, 2.1-4 cm and 4.1-6 cm. Electrical conductivity of top 0-2 cm soil depth was 20.49 dS/m, in 2.1-4 cm soil depth was 7.14 dS/m and in 4.1-6 cm soil depth 4.15 dS/m. The study soils were strongly acidic having pH value 4.73, 4.99 and 5.20 in 0-2, 2.1-4 and 4.1-6 cm soil depth, respectively. The highest of 8.8 Na:K ratio was found in 0-2 cm soil depth. The Na:K ratio gradually decreased with the increase of soil depth, having 6.59 in 2.1-4 cm and 5.42. in 4.1-6 cm soil depth. The results clearly reveal that the top soil is very much sensitive to salt stress. Based on the electrical conductivity and Na:K ratio the Fashipara, Kuakata and Tajepara village were found seriously affected by salinity.Journal of Bangladesh Academy of Sciences, Vol. 42, No. 1, 11-23, 2018

A Functional Alternative Oxidase Modulates Plant Salt Tolerance in Physcomitrella patens

2019 ◽  
Vol 60 (8) ◽  
pp. 1829-1841 ◽  
Author(s):  
Guochun Wu ◽  
Sha Li ◽  
Xiaochuan Li ◽  
Yunhong Liu ◽  
Shuangshuang Zhao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Alternative Oxidase ◽  
Physcomitrella Patens ◽  
Redox Homeostasis ◽  
Respiratory Pathway ◽  
Functional Link ◽  
Salt Stress Condition ◽  
Plant Salt Tolerance

Abstract Alternative oxidase (AOX) has been reported to be involved in mitochondrial function and redox homeostasis, thus playing an essential role in plant growth as well as stress responses. However, its biological functions in nonseed plants have not been well characterized. Here, we report that AOX participates in plant salt tolerance regulation in moss Physcomitrella patens (P. patens). AOX is highly conserved and localizes to mitochondria in P. patens. We observed that PpAOX rescued the impaired cyanide (CN)-resistant alternative (Alt) respiratory pathway in Arabidopsis thaliana (Arabidopsis) aox1a mutant. PpAOX transcription and Alt respiration were induced upon salt stress in P. patens. Using homologous recombination, we generated PpAOX-overexpressing lines (PpAOX OX). PpAOX OX plants exhibited higher Alt respiration and lower total reactive oxygen species accumulation under salt stress condition. Strikingly, we observed that PpAOX OX plants displayed decreased salt tolerance. Overexpression of PpAOX disturbed redox homeostasis in chloroplasts. Meanwhile, chloroplast structure was adversely affected in PpAOX OX plants in contrast to wild-type (WT) P. patens. We found that photosynthetic activity in PpAOX OX plants was also lower compared with that in WT. Together, our work revealed that AOX participates in plant salt tolerance in P. patens and there is a functional link between mitochondria and chloroplast under challenging conditions.

scholarly journals The Arabidopsis glutathione transferases, AtGSTF8 and AtGSTU19 are involved in the maintenance of root redox homeostasis affecting meristem size and salt stress sensitivity

Plant Science ◽  
2019 ◽  
Vol 283 ◽  
pp. 366-374 ◽  
Author(s):  
Edit Horváth ◽  
Krisztina Bela ◽  
Botond Holinka ◽  
Riyazuddin Riyazuddin ◽  
Ágnes Gallé ◽  
...  
Keyword(s):  
Salt Stress ◽  
Redox Homeostasis ◽  
Stress Sensitivity ◽  
Glutathione Transferases ◽  
Meristem Size

Proteomic analysis of Mesembryanthemum crystallinum leaf microsomal fractions finds an imbalance in V-ATPase stoichiometry during the salt-induced transition from C3 to CAM

2013 ◽  
Vol 450 (2) ◽  
pp. 407-415 ◽  
Author(s):  
Cristian Cosentino ◽  
Dario Di Silvestre ◽  
Elke Fischer-Schliebs ◽  
Ulrike Homann ◽  
Antonella De Palma ◽  
...  
Keyword(s):  
Salt Stress ◽  
Protein Composition ◽  
Mesembryanthemum Crystallinum ◽  
Strong Increase ◽  
Salt Adaptation ◽  
Label Free ◽  
Atpase Subunit ◽  
Proteomic Data ◽  
Subunit Stoichiometry

The halophyte Mesembryanthemum crystallinum adapts to salt stress by salt uptake and switching from C3 photosynthesis to CAM (crassulacean acid metabolism). An important role in this process is played by transport proteins in the tonoplast of the central vacuole. In the present study we examine dynamic changes in the protein composition during salt-stress adaptation in microsomes from M. crystallinum leaves. Plants challenged with 400 mM NaCl accumulate salt by day 4 of treatment and malic acid only at day 12; a switching to CAM hence follows any initial steps of salt adaptation with a delay. Using a label-free and semiquantitative approach, we identified the most dramatic changes between the proteome of control plants and plants harvested after 12 days of the treatment; the abundance of 14 proteins was significantly affected. The proteomic data revealed that the majority of the subunits of V-ATPase (vacuolar H+-ATPase) holoenzyme. The salt treatment somewhat decreased the abundance of all subunits in the short term (4 days). Long-term adaptation, including the switching to CAM, goes together with a strong increase in the representation of all detectable subunits. Because this increase is subunit-specific, with the highest rise occurring for subunits E and c, the data suggest that long-term adaptation to salt stress correlates with a change in V-ATPase subunit stoichiometry and highlight the structural plasticity of this holoenzyme.

scholarly journals Quantitative Proteome and PTMome Analysis of Arabidopsis Thaliana Root Responses to Persistent Osmotic and Salinity Stress.

2021 ◽  
Author(s):  
M C Rodriguez ◽  
D Mehta ◽  
M Tan ◽  
R G Uhrig
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Stress Responses ◽  
Protein Level ◽  
Economic Losses ◽  
Plant Responses ◽  
Protein Abundance ◽  
Lysine Acetylation ◽  
Label Free ◽  
Proteome Level

ABSTRACT Abiotic stresses such as drought result in large annual economic losses around the world. As sessile organisms, plants cannot escape the environmental stresses they encounter, but instead must adapt to survive. Studies investigating plant responses to osmotic and/or salt stress have largely focused on short-term systemic responses, leaving our understanding of intermediate to longer-term adaptation (24 h - days) lacking. In addition to protein abundance and phosphorylation changes, evidence suggests reversible lysine acetylation may also be important for abiotic stress responses. Therefore, to characterize the protein-level effects of osmotic and salt stress, we undertook a label-free proteomic analysis of Arabidopsis thaliana roots exposed to 300 mM Mannitol and 150 mM NaCl for 24 h. We assessed protein phosphorylation, lysine acetylation and changes in protein abundance, detecting significant changes in 245, 35 and 107 total proteins, respectively. Comparison with available transcriptome data indicates that transcriptome- and proteome-level changes occur in parallel, while PTMs do not. Further, we find significant changes in PTMs and protein abundance involve different proteins from the same networks, indicating a multifaceted regulatory approach to prolonged osmotic and salt stress. In particular, we find extensive protein-level changes involving sulphur metabolism under both osmotic and salt conditions as well as changes in protein kinases and transcription factors that may represent new targets for drought stress signaling. Collectively, we find that protein-level changes continue to occur in plant roots 24 h from the onset of osmotic and salt stress and that these changes differ across multiple proteome levels.

scholarly journals Comparative Proteomic Analysis of Tolerant and Sensitive Varieties Reveals That Phenylpropanoid Biosynthesis Contributes to Salt Tolerance in Mulberry

2021 ◽  
Vol 22 (17) ◽  
pp. 9402
Author(s):  
Tiantian Gan ◽  
Ziwei Lin ◽  
Lijun Bao ◽  
Tian Hui ◽  
Xiaopeng Cui ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Molecular Mechanism ◽  
Calcium Content ◽  
Heavy Metal Stress ◽  
Sodium Content ◽  
Proline Content ◽  
Salt Tolerant ◽  
Phenylpropanoid Biosynthesis ◽  
Selection Of

Mulberry, an important woody tree, has strong tolerance to environmental stresses, including salinity, drought, and heavy metal stress. However, the current research on mulberry resistance focuses mainly on the selection of resistant resources and the determination of physiological indicators. In order to clarify the molecular mechanism of salt tolerance in mulberry, the physiological changes and proteomic profiles were comprehensively analyzed in salt-tolerant (Jisang3) and salt-sensitive (Guisangyou12) mulberry varieties. After salt treatment, the malondialdehyde (MDA) content and proline content were significantly increased compared to control, and the MDA and proline content in G12 was significantly lower than in Jisang3 under salt stress. The calcium content was significantly reduced in the salt-sensitive mulberry varieties Guisangyou12 (G12), while sodium content was significantly increased in both mulberry varieties. Although the Jisang3 is salt-tolerant, salt stress caused more reductions of photosynthetic rate in Jisang3 than Guisangyou12. Using tandem mass tags (TMT)-based proteomics, the changes of mulberry proteome levels were analyzed in salt-tolerant and salt-sensitive mulberry varieties under salt stress. Combined with GO and KEGG databases, the differentially expressed proteins were significantly enriched in the GO terms of amino acid transport and metabolism and posttranslational modification, protein turnover up-classified in Guisangyou12 while down-classified in Jisang3. Through the comparison of proteomic level, we identified the phenylpropanoid biosynthesis may play an important role in salt tolerance of mulberry. We clarified the molecular mechanism of mulberry salt tolerance, which is of great significance for the selection of excellent candidate genes for saline-alkali soil management and mulberry stress resistance genetic engineering.

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