scholarly journals Eutypella parasitica and Other Frequently Isolated Fungi in Wood of Dead Branches of Young Sycamore Maple (Acer pseudoplatanus) in Slovenia

Forests ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 467 ◽  
Author(s):  
Ana Brglez ◽  
Barbara Piškur ◽  
Nikica Ogris
Keyword(s):  
Species Diversity ◽  
Fungal Community ◽  
Sampling Site ◽  
Outer Part ◽  
Similarity Index ◽  
Asymptomatic Infection ◽  
Fungal Species ◽  
Acer Pseudoplatanus ◽  
Pure Cultures ◽  
Sycamore Maple

Eutypella parasitica R.W. Davidson and R.C. Lorenz is the causative agent of Eutypella canker of maple, a destructive disease of maples in Europe and North America. The fungus E. parasitica infects the trunk through a branch stub or bark wound. Because the fungal community may have an impact on infection and colonization by E. parasitica, the composition of fungi colonizing wood of dead branches of sycamore maple (Acer pseudoplatanus L.) was investigated in five sampling sites in Slovenia. Forty samples from each sampling site were collected between the November 2017 and March 2018 period. Isolations were made from the wood in the outer part of dead branches and from discoloured wood in the trunk that originated from a dead branch. Pure cultures were divided into morphotypes, and one representative culture per morphotype was selected for further molecular identification. From a total of 2700 cultured subsamples, 1744 fungal cultures were obtained, which were grouped into 212 morphotypes. The investigated samples were colonized by a broad spectrum of fungi. The most frequently isolated species were Eutypa maura (Fr.) Sacc., Eutypa sp. Tul. and C. Tul., Fusarium avenaceum (Fr.) Sacc., Neocucurbitaria acerina Wanas., Camporesi, E.B.G. Jones and K.D. Hyde and E. parasitica. In this study, we distinguished species diversity and the fungal community. There were no significant differences in the diversity of fungal species between the five sampling sites, and branch thickness did not prove to be a statistically significant factor in fungal species diversity. Nevertheless, relatively low Jaccard similarity index values suggested possible differences in the fungal communities from different sampling sites. This was confirmed by an analysis of similarities, which showed that the isolated fungal community distinctly differed between the five sampling sites and between the different isolation sources. Eutypella parasitica was isolated from all five investigated sampling sites, although Eutypella cankers were observed in only three sampling sites, indicating the possibility of asymptomatic infection.

scholarly journals Keanekaragaman dan Kelimpahan Belalang dan Kerabatnya (Orthoptera) pada Dua Ekosistem Pegunungan di Taman Nasional Gunung Halimun-Salak

2015 ◽  
Vol 7 (2) ◽  
pp. 100
Author(s):  
NETY VIRGO ERAWATI ◽  
SIH KAHONO
Keyword(s):  
Species Richness ◽  
Species Diversity ◽  
National Park ◽  
Diversity Index ◽  
Sampling Site ◽  
Similarity Index ◽  
Line Transect ◽  
Light Traps ◽  
Set Up ◽  
Sweep Net

A study on diversity and abundance of grasshopper and its relatives (Orthoptera) was conducted at two mountainous rainforest ecosystems (Mounts Kendeng and Botol) of Gunung Halimun-Salak National Park. A hundred meters of a line transect was used to sample and set up several insect traps (yellow pan, malaise, pit fall, bait pit fall, sweep net, and light traps), and insect sweepings as well. The light traps were set up at about fifty meters distance from the end of the sampling sites. A total individual collected by traps was combined on every comparable sampling site. Total individuals of the Orthoptera captured were 414; consisted of 25 species of 9 families. Both species diversity and number of families were higher at Mount Kendeng rather than Mount Botol. Number of species of each family usually similar except on family of Grillidae was much higher at Mount Kendeng. Species belong to Phasmidae was not recorded at Mount Kendeng, while species belong to both families of Gryllotalpidae and Tettigonidae were not captured at Mount Botol as well. Overal there was a difference in the species richness at each between. Shannon Diversity Index (H’) and evenness (E) were higher at Mount Kendeng (2.44 and 0.81) rather than Mount Botol (1.80 and 0.66). Similarity Index of Jaccard (Cj) and Sorenson (Cn) of both localities were similar (0.40 and 0.32). Herbivores were most dominant at both localities (Phasmidae, Tetrigidae, Acrididae, Gryllidae, dan Gryllotalpidae), followed by omnivores (Blattidae), scavenger (Gryllacrididae), and predator (Mantidae).

scholarly journals Ectomycorrhizal fungal community structure in a young orchard of grafted and ungrafted hybrid chestnut saplings

Mycorrhiza ◽  
2021 ◽  
Vol 31 (2) ◽  
pp. 189-201
Author(s):  
Serena Santolamazza-Carbone ◽  
Laura Iglesias-Bernabé ◽  
Esteban Sinde-Stompel ◽  
Pedro Pablo Gallego
Keyword(s):  
Species Diversity ◽  
Fungal Community ◽  
Sequence Data ◽  
Early Stage ◽  
Castanea Sativa ◽  
Fungal Species ◽  
Colonization Rate ◽  
Root Tips ◽  
Scleroderma Citrinum ◽  
The Impact

AbstractEctomycorrhizal (ECM) fungal community of the European chestnut has been poorly investigated, and mostly by sporocarp sampling. We proposed the study of the ECM fungal community of 2-year-old chestnut hybrids Castanea × coudercii (Castanea sativa × Castanea crenata) using molecular approaches. By using the chestnut hybrid clones 111 and 125, we assessed the impact of grafting on ECM colonization rate, species diversity, and fungal community composition. The clone type did not have an impact on the studied variables; however, grafting significantly influenced ECM colonization rate in clone 111. Species diversity and richness did not vary between the experimental groups. Grafted and ungrafted plants of clone 111 had a different ECM fungal species composition. Sequence data from ITS regions of rDNA revealed the presence of 9 orders, 15 families, 19 genera, and 27 species of ECM fungi, most of them generalist, early-stage species. Thirteen new taxa were described in association with chestnuts. The basidiomycetes Agaricales (13 taxa) and Boletales (11 taxa) represented 36% and 31%, of the total sampled ECM fungal taxa, respectively. Scleroderma citrinum, S. areolatum, and S. polyrhizum (Boletales) were found in 86% of the trees and represented 39% of total ECM root tips. The ascomycete Cenococcum geophilum (Mytilinidiales) was found in 80% of the trees but accounted only for 6% of the colonized root tips. These results could help to unveil the impact of grafting on fungal symbionts, improving management of chestnut agro-ecosystems and production of edible fungal species.

scholarly journals Development of a Co-Dominant Cleaved Amplified Polymorphic Sequences Assay for the Rapid Detection and Differentiation of Two Pathogenic Clarireedia spp. Associated with Dollar Spot in Turfgrass

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1489
Author(s):  
Tammy Stackhouse ◽  
Sumyya Waliullah ◽  
Alfredo D. Martinez-Espinoza ◽  
Bochra Bahri ◽  
Emran Ali
Keyword(s):  
Pcr Primers ◽  
The United States ◽  
Fungal Species ◽  
Dollar Spot ◽  
Direct Pcr ◽  
Specific Pcr ◽  
Specificity And Sensitivity ◽  
Pure Cultures ◽  
Speed Up ◽  
Cleaved Amplified Polymorphic Sequences

Dollar spot is one of the most destructive diseases in turfgrass. The causal agents belong to the genus Clarireedia, which are known for causing necrotic, sunken spots in turfgrass that coalesce into large damaged areas. In low tolerance settings like turfgrass, it is of vital importance to rapidly detect and identify the pathogens. There are a few methods available to identify the genus Clarireedia, but none of those are rapid enough and characterize down to the species level. This study produced a co-dominant cleaved amplified polymorphic sequences (CAPS) test that differentiates between C. jacksonii and C. monteithiana, the two species that cause dollar spot disease within the United States. The calmodulin gene (CaM) was targeted to generate Clarireedia spp. specific PCR primers. The CAPS assay was optimized and tested for specificity and sensitivity using DNA extracted from pure cultures of two Clarireedia spp. and other closely related fungal species. The results showed that the newly developed primer set could amplify both species and was highly sensitive as it detected DNA concentrations as low as 0.005 ng/µL. The assay was further validated using direct PCR to speed up the diagnosis process. This drastically reduces the time needed to identify the dollar spot pathogens. The resulting assay could be used throughout turfgrass settings for a rapid and precise identification method in the US.

scholarly journals Fine-scale spatial genetic structure of sycamore maple (Acer pseudoplatanus L.)

2011 ◽  
Vol 131 (3) ◽  
pp. 739-746 ◽  
Author(s):  
Madhav Pandey ◽  
Oliver Gailing ◽  
Hans H. Hattemer ◽  
Reiner Finkeldey
Keyword(s):  
Genetic Structure ◽  
Spatial Genetic Structure ◽  
Acer Pseudoplatanus ◽  
Fine Scale ◽  
Sycamore Maple

scholarly journals Growth performance and wood structure of wavy grain sycamore maple (Acer pseudoplatanus L.) in a progeny trial

2021 ◽  
Vol 78 (1) ◽  
Author(s):  
Mona Quambusch ◽  
Cornelia Bäucker ◽  
Volker Haag ◽  
Andreas Meier-Dinkel ◽  
Heike Liesebach
Keyword(s):  
Growth Performance ◽  
Vegetative Propagation ◽  
Seed Orchard ◽  
Breeding Systems ◽  
Acer Pseudoplatanus ◽  
Wood Structure ◽  
Progeny Trial ◽  
Plus Tree ◽  
Sycamore Maple ◽  
Figure Type

Abstract • Key message Wavy grain, a rare figure type of wood, leads to highly priced timber in Acer pseudoplatanus L. The influence of this trait on growth performance and its causes are not known. Analyzed wavy and straight grain sycamore maple progenies show comparable growth performance in a field trial. Stability of wavy grain after vegetative propagation is confirmed and genetic inheritance indicated. • Context Wavy grain is a rare figure type of wood resulting from undulating fiber growth that leads to a decorative and highly priced timber in Acer pseudoplatanus L. with top positions on auction sales. Nevertheless, neither the influence of this trait on growth performance is known, nor have the causes been disentangled. • Aims Our objectives were to find out if wavy grain figure influences growth parameters essential for log quality and to gain insight into the causes of wavy grain by the analysis of a progeny trial and a seed orchard. • Methods In a progeny trial with 30-year-old F-1-offspring from selected wavy grained and straight grained trees, trunk diameter, tree height, and trunk shape were evaluated. Additionally, 21 trees of the trial and selected plus tree-grafts of a seed orchard were felled and analyzed for occurrence and intensity of wavy grain structure. • Results No effect of the wavy and straight grain parentage on growth was observed in the progeny trial. Of the felled trees, over 30% showed evidence of wavy grain compared to rare occurrence in natural stands. Wood structure analysis of plus tree scions confirmed the stability of wavy grain after vegetative propagation. • Conclusion Wavy grain seems to be genetically inherited, and there seems to be no statistically significant difference in commercially relevant traits in the progeny. This highlights the value of including wavy grain as a desired attribute in breeding systems of maple.

The Mycobiota of Speck, a Traditional Tyrolean Smoked and Cured Ham

2000 ◽  
Vol 63 (10) ◽  
pp. 1399-1403 ◽  
Author(s):  
URSULA PEINTNER ◽  
JOHANNES GEIGER ◽  
REINHOLD PÖDER
Keyword(s):  
Species Diversity ◽  
Fungal Species ◽  
Penicillium Canescens ◽  
Penicillium Verrucosum ◽  
South Tyrol ◽  
Cured Meat ◽  
Penicillium Commune ◽  
Penicillium Solitum ◽  
Penicillium Spp

Speck is a ham specialty product traditionally produced in South Tyrol (Italy) and North Tyrol (Austria) by farmers, butcheries, and meat industries. To date, nothing has been learned about fungi associated with this smoked and cured meat. Therefore, it was the main objective of this study to assess the typical mycobiota of Speck in relation to the different production types and the geographic provenance. A total of 121 Speck samples from North Tyrol and South Tyrol was analyzed. From 63 isolated fungal species, only a few can be regarded as typical colonizers: Eurotium rubrum and Penicillium solitum were the dominating species in all types and parts of Speck (crust, meat, and fat). Eight other Penicillium spp. were relatively frequent. The species diversity increased from industrially produced Speck to products from butcheries and farmers, and it was higher in all types of South Tyrolean products. Among the typical mycobiota, Penicillium verrucosum, Penicillium canescens, and Penicillium commune are known as potentially mycotoxigenic.

scholarly journals ANALISIS VEGETASI DI PULAU BINTAN, KEPULAUAN RIAU

2019 ◽  
Vol 18 (3) ◽  
Author(s):  
Bayu Arief Pratama ◽  
Edi Mirmanto
Keyword(s):  
Species Diversity ◽  
Ecological Study ◽  
Similarity Index ◽  
Basal Area ◽  
Small Islands ◽  
Vegetation Analysis ◽  
Strong Relation ◽  
Study Sites ◽  
Riau Archipelago ◽  
Common Tree

Ecological study with special attention to vegetation analysis has been done in the Bintan forest area, Riau Archipelago. Based on three sampling plots of 30 × 30 m, there were 73 tree (dbh ≥ 5 cm) species were recorded, belong to 64 genera and 32 families. The density is fairly high (1,485 individuals /ha) with total basal area relatively low (27.71 m2/ha).  Its indicates that most of trees are small size. Similarity index among study sites are relatively low (< 25 %), indicates that there is strong relation between habitat and species composition. Out of the 6 most common tree species in the study area, only Dipterocarpus gracillis was dominant in 2 study sites. Overall species diversity is relatively low, which is characteristic of forest vegetation on small islands. 

scholarly journals THE MACROFUNGI DIVERSITY AND THEIR POTENTIAL UTILIZATION IN TANGALE NATURE RESERVE GORONTALO PROVINCE

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Diah Irawati Dwi Arini ◽  
Margaretta Christita ◽  
Julianus Kinho
Keyword(s):  
Species Diversity ◽  
Nature Reserve ◽  
Edible Mushroom ◽  
Fungal Species ◽  
Conservation Area ◽  
Scleroderma Citrinum ◽  
Lenzites Betulina ◽  
The Veil ◽  
Decaying Wood ◽  
Potential Use

Tangale Nature reserve is a conservation area located in Gorontalo that have the biodiversity typical of Wallacea bioregion including macroscopic fungi. The purpose of this study was to identify the fungal species diversity found in the Tangale Nature reserve as well as its potential use, considering that the information on species diversity of fungi in the Wallacea region is very limited. This research was conducted using the cruise method by identifying macroscopic fungi along the hiking trail of Tangale Nature Reserve. The macroscopic fungi were observed for its morphological characteristic include the colour, diameter, surface of the veil, the shape of the stem, the length and diameter of the stem, the lamella, including ring and pore, type of lamella and the type of volva. The research recorded the substrates and the location where the fungus was found. Data were analyzed descriptively qualitative. The results of the research identified 28 species of macroscopic fungi that included to the division Ascomycota and Basidiomycota. It belongs to 16 families and 8 orders  are Pezizales, Agaricales, Auriculariales, Boletales, Cantharellales, Gomphales, Polyporales, dan Russulales. Based on place to grow as much as 57,14% were found growing on decaying wood and 42,86% found growing in the soil/litter. Based on the potential of use, it has identified four species of mushrooms potentially as edible mushroom are Pleurotus ostreatus,  Auricularia auricula, Ramaria formosa, and Polyporus arcularius. 11 species of mushrooms potentially as medicinal mushroom are Calvatia craniiformis, Scleroderma citrinum, Lenzites betulina, Microporus flabelliformis, Coriolus versicolor, Microporus xanthopus, and Albatrellus confluens.  

scholarly journals The The diversity and abundance of Hymenoptera insects on tidal swamp rice field in Indragiri Hilir District, Indonesia

2020 ◽  
Vol 21 (3) ◽  
Author(s):  
ZAHLUL IKHSAN ◽  
HIDRAYANI ◽  
YAHERWANDI ◽  
HASMIANDY HAMID
Keyword(s):  
Species Diversity ◽  
Rice Field ◽  
Diversity Index ◽  
Human Life ◽  
Similarity Index ◽  
Pitfall Trap ◽  
Vital Role ◽  
Tidal Swamp ◽  
Abundance And Diversity ◽  
Number Of Individuals

Abstract. Ikhsan Z, Hidrayani, Yaherwandi, Hamid H. 2020. The diversity and abundance of Hymenoptera insects on tidal swamp rice field in Indragiri Hilir District, Indonesia. Biodiversitas 21: 1020-1026. Hymenoptera is one of the four largest insect orders and it plays a vital role in human life as pollinators, parasitoids, and predators. Hymenoptera parasitoids are particularly important as agents of biological control of pest. The objective of this research was to study the diversity and abundance of Hymenoptera on tidal swamp rice field in Indragiri Hilir District, Riau Province, Indonesia. Samplings were conducted in 3 sub-districts of rice production centers, namely Batang Tuaka, Keritang and Reteh, using four trapping techniques (i.e. insect net, malaise trap, pitfall trap, and yellow pan trap). Shannon diversity index (H '), Krebs evenness index (E), and Jaccard similarity index (Is) were calculated. We found 4,701 individuals consisting of 39 families and 319 species of Hymenoptera at the three studied areas. Tidal swamp rice in Indragiri Hilir District had a high species diversity of Hymenoptera parasitoids and Hymenoptera predators, but a moderate species diversity of Hymenoptera pollinators. Species diversity and evenness of Hymenoptera parasitoids and predators were higher in Keritang than in Batang Tuaka and Reteh. The families of Formicidae, Braconidae, Ichneumonidae, and Scelionidae had the highest number of species, while Formicidae, Scelionidae, Diapriidae, and Braconidae had the highest number of individuals. Based on these findings, the abundance and diversity of Hymenoptera insects in Indragiri Hilir District have the potentials to be developed as biological agents to control pests on tidal swamp rice in this area.

scholarly journals KEANEKARAGAMAN JENIS TUMBUHAN PAKU-PAKUAN (PTERIDOPHYTA) DAN KONDISI TEMPAT TUMBUHNYA PADA HUTAN RAWA GAMBUT SEKUNDER DAN LAHAN GAMBUT TERBUKA

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Furwoko Kirno ◽  
Dwi Astiani ◽  
Hanna Artuti Ekamawanti
Keyword(s):  
Species Diversity ◽  
Diversity Index ◽  
Similarity Index ◽  
Survey Method ◽  
Site Conditions ◽  
Swamp Forest ◽  
Peat Swamp Forest ◽  
Plot Size ◽  
Peat Swamp ◽  
Index Value

The activities of the community around the peat swamp forest cause disruption of the ecosystem from the sustainability of the forest area's resources. Land clearing and peatland fires also affect the presence of ferns. The new environmental conditions due to changes in land cover cause ferns to be affected by these changes. Kuala Dua Village Kubu Raya Regency has secondary peat swamp forest and open peat land due to fires 3-4 years earlier so it is overgrown with a variety of new types of flora, one of which is a type of fern. The objective of the study is to describe species diversity of ferns and their site conditions in secondary peat swamp forests and open peatlands of Kuala Dua Village, Kubu Raya District. This research was conducted with survey method using multiple plot samples. The plots determination was done by purposive sampling which was taken place on the site where there were many species of ferns land. Three plots were on each land covered condition with a plot size of 5 m x 5 m that divided into 25 of a 1 m x 1 m sub plots. The results show that 5 species of ferns found in peat swamp forest and 4 species of ferns were found on open peatlands. The species that has the highest INP value in the forest was Asplenium belangeri and the species that has the INP value in open peatland was Nephrolefis falcata. Dominant Index, Spesies Diversity Index and Spesies Abudance Index were 0,12 and 0,18; 0,30 and 0,36; 0,51 and 0,52 on the forest and open peatland respectively. The Similarity Index value (IS) of ferns in secondary peat swamp forest and open peatland is 44%. The study shows dissimility of species fern in peat swamp forests and open peatlands.Keywords: Open peatland, Pteridophyta, secondary peat swamp forest

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