scholarly journals Sargassum Inundations in Turks and Caicos: Methane Potential and Proximate, Ultimate, Lipid, Amino Acid, Metal and Metalloid Analyses

Energies ◽  
2020 ◽  
Vol 13 (6) ◽  
pp. 1523 ◽  
Author(s):  
John James Milledge ◽  
Supattra Maneein ◽  
Elena Arribas López ◽  
Debbie Bartlett
Keyword(s):  
Amino Acid ◽  
Biogas Production ◽  
Dry Weight ◽  
World Health Organisation ◽  
Amino Acid Profile ◽  
World Health ◽  
Individual Species ◽  
Methane Potential ◽  
The Individual ◽  
Calcium Iron

The Caribbean has been experiencing beach inundations of pelagic Sargassum, causing environmental, health and financial issues. This study showed variations in the composition and methane potential (MP) between the species of Sargassum. The MPs for S. natans VIII, S. natans I and S. fluitans (145, 66 and 113 mL CH4 g−1 Volatile Solids) were considerably below theoretical potentials, possibly due to the high levels of indigestible fibre and inhibitors. The mixed mats Sargassum composition was substantially different from the individual species, being higher in ash, calcium, iron, arsenic and phenolics. The mixed mats produced no methane, perhaps due to the high levels of phenolics. There was a strong correlation between MP and phenolic content. Heavy metals and metalloids were at levels that should not cause concern, except for arsenic (21–124 mg kg−1 dry weight). Further work on the speciation of arsenic in Sargassum is required to fully determine the risk to health and agriculture. Both protein and lipid levels were low. The ‘indispensable amino acid’ profile compares favourably with that recommended by the World Health Organisation. Lipids had a high proportion of Polyunsaturated Fatty Acids. The use of Sargassum for biogas production could be challenging, and further work is required.

scholarly journals Proximate composition, mineral content and amino acid profile of Irvingia gabonensis O’Rorke baill leaf

2016 ◽  
Vol 5 (1) ◽  
pp. 23
Author(s):  
Efosa Ewere ◽  
Oboso Etim ◽  
Usunomena Usunobun
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Proximate Composition ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Antinutritional Factors ◽  
World Health ◽  
Isoleucine Leucine ◽  
Irvingia Gabonensis ◽  
Mineral Analyses

Several plants are utilized for medicinal and nutritional purposes. Irvingia gabonensis O’Rorke Baill leaf is used in herbal medicine for treatment of a number of ailments. This study was therefore carried out to investigate the proximate composition, antinutritional factors, mineral composition and amino acid profile of Irvingia gabonensis O’Rorke Baill leaf. The proximate and antinutritional factors analyses were done using standard procedures. The mineral analyses were done using flame photometry, titrimetic method, molybdo vanadate method and atomic absorption spectrophotometry and the amino acid profile was done with the aid of Applied Biosystems PTH amino acid analyzer. Results of proximate analyses were carbohydrates (75.15±1.29 %), protein (11.43±1.07 %), fat (1.99±0.74 %), fibre (4.89±0.61 %), ash (6.71±0.28 %), moisture (5.12±0.03 %) and caloric value (364.30±5.95 Kcal). Antinutrients (phytate, oxalate and cyanide) levels in the leaf were also very low. Results from mineral analyses obtained revealed that the leaf is also a very rich source of calcium, potassium, sodium, magnesium and so on. Compared with the World health organization (WHO) standards, results of the amino acid profile showed that the leaf is very rich in isoleucine, leucine, lysine, phenylalanine, threonine, valine and tyrosine which are nutritionally essential amino acids. Furthermore, extraction of the leaf using ethanol reduced the levels of these amino acids but not below the recommended WHO standard levels for most of the essential amino acids. Irvingia gabonensis O’Rorke Baill leaf is therefore a potential source of key nutrients.

scholarly journals Nutrient Composition of Dried Seaweed Gracilaria gracilis

2019 ◽  
Vol 24 (1) ◽  
pp. 1 ◽  
Author(s):  
Abdullah Rasyid ◽  
Ardi Ardiansyah ◽  
Ratih Pangestuti
Keyword(s):  
Fatty Acid ◽  
Amino Acid ◽  
Dietary Fiber ◽  
Essential Amino Acid ◽  
Dry Weight ◽  
Amino Acid Profile ◽  
Nutrient Composition ◽  
Raw Material ◽  
Gracilaria Gracilis ◽  
South Sulawesi

The nutrient composition of dried red seaweed Gracilaria gracilis collected from Barru waters, South Sulawesi including proximate, dietary fiber, minerals, fatty acid and amino acid profile has been investigated. The objective of this study was to evaluate the various nutritional parameters of G. gracilis for utilization in human nutrition. Results show that the content of moisture (19.045), protein (10.86%), ash (6.78%), fat (0.18%), carbohydrate (63.13%) and dietary fiber (27.48%) basis on the dry weight. The content of calcium (429.11 mg.100 g-1), sodium (290.89 mg.100 g-1), phosphor (57.01 mg.100 g-1), iron (15.20 mg.100 g-1) and potassium (1380.42 mg.100 g-1). Leucine was the major essential amino acid found to be 9374.22 mg.kg-1, while glutamic acid was the major non-essential amino acid found to be 10848.98 mg.kg-1. Palmitic acid was the major saturated fatty acid found to be 0.08%, while oleic acid was the major unsaturated fatty acid found to be 0.05%. The nutrient composition of G. gracilis was discussed in this study and suggested that the seaweed species have potentially be used as raw material or ingredient of a healthy food for human.

scholarly journals Heterogeneity of rat goblet-cell mucin before and after reduction

1983 ◽  
Vol 209 (1) ◽  
pp. 117-124 ◽  
Author(s):  
R E F Fahim ◽  
G G Forstner ◽  
J F Forstner
Keyword(s):  
Amino Acid ◽  
Goblet Cell ◽  
Sodium Dodecyl ◽  
Amino Acid Profile ◽  
Heterogeneous Structure ◽  
Silver Stain ◽  
Low Concentrations ◽  
Before And After ◽  
The Individual ◽  
Sepharose 4B

Goblet-cell mucin of rat small intestine was purified from mucosal scrapings by using centrifugation, Sepharose 4B and Sepharose 2B chromatography. The mucin was applied in low concentrations (1 microgram/track) to slab gels containing 0.5% agarose/2% (w/v) polyacrylamide, and bands were detected after electrophoresis by silver stain or by fluorography of 3H-labelled mucin. Before reduction the mucin contained three distinct components: a polymeric species at the top of the gel and two large glycoproteins of higher mobility. After reduction, the polymer disappeared, the two glycoproteins remained unchanged, and two glycopeptide bands of higher mobility appeared. In addition, a non-glycosylated, heavily stained peptide of mol.wt. 118000 was detected. The individual mucin components were partially separated on Sepharose 2B, 0.2M-NaCl/1% sodium dodecyl sulphate being used as eluant. Individual amino acid and carbohydrate analyses suggested that the glycosylated components, despite their differences in size, had identical profiles. The 118000-mol.wt. peptide had a very different amino acid profile, with much less serine, threonine and proline. Glycine and aspartic and glutamic acids comprised 34% of the total amino acids. Thus the ‘native’ mucin is a heterogeneous structure containing at least two non-covalently associated glycoproteins plus polymeric material. The latter is stabilized by disulphide bonds and consists of several glycopeptides of different size as well as a ‘link’ peptide of mol.wt. 118000.

Amino acids in the octocoralVeretillum cynomorium: the effect of seasonality and differences from scleractinian hexacorals

2012 ◽  
Vol 93 (4) ◽  
pp. 913-918 ◽  
Author(s):  
Miguel Baptista ◽  
Ana Luísa Maulvault ◽  
Katja Trübenbach ◽  
Luis Narciso ◽  
António Marques ◽  
...  
Keyword(s):  
Amino Acid ◽  
Principal Component ◽  
Dry Weight ◽  
Amino Acid Profile ◽  
Temporal Variations ◽  
Total Amino Acid ◽  
Interspecific Differences ◽  
Biochemical Studies ◽  
Almost All ◽  
First Time

The majority of biochemical studies in corals has been focused on the lipidic composition and little attention has been given to the amino acid profile of these invertebrates. The objectives of this work were to investigate, for the first time, the temporal variations in the total amino acid (AA) composition of an octocoral, namely the sea penVeretillum cynomorium, and to evaluate possible interspecific differences in AA profile between this octocoral and hexacorals. The quantitatively most important AAs inV. cynomoriumcolonies were: glutamic acid, varying from 3.92 to 5.94% dry weight (dw) and representing around 14–15% of total AA content; aspartic acid (3.34–4.99% dw; 11–12%); and glycine (2.87–4.57% dw; 9–12%). On the other hand, the minor AAs were methionine (0.41–0.73% dw; 1–2%) and histidine (0.54–0.76% dw; 2%). Almost all AAs showed the same significant seasonal variations, with the highest values in February, second highest in October and the lowest in June. Some AAs, namely lysine, phenylalanine and methionine did not follow this trend and showed the major peak in October. Most of the AA variations seemed to be linked to changes in food availability and/or gametogenesis. Principal component analysis clearly separated the octocoral from the group of hexacorals, mainly due to the higher percentages of arginine, tyrosine and glycine inV. cynomorium, and valine, serine, histidine, isoleucine and alanine in hexacorallia species. We speculate that this differentiation possibly derived from physiological differences related to phylogeny, and was not affected by reproductive or environmental seasonality.

scholarly journals Fatty and amino acid profile of mealworm larvae (Tenebrio molitor L.)

2020 ◽  
Vol 36 (2) ◽  
pp. 167-180
Author(s):  
Igor Jajic ◽  
Aleksandra Popovic ◽  
Miroslav Urosevic ◽  
Sasa Krstovic ◽  
Milos Petrovic ◽  
...  
Keyword(s):  
Amino Acids ◽  
Fatty Acid ◽  
Amino Acid ◽  
Animal Feed ◽  
Amino Acid Content ◽  
Dry Weight ◽  
Essential Amino Acids ◽  
Tenebrio Molitor ◽  
Amino Acid Profile ◽  
Scale Production

The yellow mealworm (Tenebrio molitor L., Coleoptera: Tenebrionidae) is an edible insect, distributed worldwide and a convenient candidate for industrial-scale production. Mealworms could be commercially used for the substitution of conventional protein sources. In our previous study, it was found that T. molitor larvae predominantly contained crude protein (55.83%) and crude fat (25.19%), as well as low content of nitrogen-free extract (based on dry weight). Mealworm specimens were maintained in an incubator under controlled conditions in plastic containers. Insects were sieved and put into the container with boiling water and cooked for 180 seconds. Moisture content was determined as weight loss after drying of larvae. Amino acids were determined on an Agilent Technologies 1260 series HPLC system. Fatty acid composition was determined on a Thermo Scientific TRACE 1300 gas chromatograph equipped with a flame ionization detector using TR-FAME column. The results showed that the content of unsaturated fatty acid is very high, i.e. oleic acid (C18:1) formed the major lipid component in 40.83%, which was followed by linoleic acid (C18:2, omega-6 fatty acid) with 29.80% and linolenic acid (C18:3) with 1.08%. The essential amino acids are highly represented in the samples (in % dry matter). This primarily refers to isoleucine (4.12), tyrosine (3.86), phenylalanine (3.06), leucine (2.96), lysine (2.67) and methionine (1.76). The differences in essential fatty and amino acid content between our results and discussed literature data, could be the consequence of different substrates used for rearing of insects. After everything stated above, the biological value of T. molitor larvae proves that it could be suitable as animal feed.

scholarly journals Comunicação em Saúde: Evitar o Contágio da Doença por Vírus Ébola nos PALOP – Metodologia KISS & KEYWORDS

2015 ◽  
Vol 28 (2) ◽  
pp. 141
Author(s):  
Isabel De Santiago ◽  
José Pereira Miguel ◽  
Francisco Antunes
Keyword(s):  
Health Communication ◽  
Ebola Virus ◽  
Virus Disease ◽  
World Health Organisation ◽  
Population Based ◽  
World Health ◽  
African Countries ◽  
Communication Plan ◽  
Sao Tome And Principe ◽  
The Individual

In this work, Health Communication is considered as an important discipline in medicine and health sciences for his role as true determinant of health. We highlight their contribution to health promotion and disease prevention. Thus, the Health Communication Plan (PCS): Preventing the spread of Ebola virus disease in the Portuguese Speaking African Countries - KISS &amp; KEYWORDS methodology is a tool that aims to minimize the risk of infection by Ebola virus in the Portuguese Speaking African Countries and also train for a general<br />improvement of health conditions of the local populations. In the PCS design are especially considered the social and cultural contexts of the target populations, especially the customs, traditions and religion. Health Communication is considered as an Essential Function of Public Health and its main is to provide a population-based approach. The target of communication actions are population groups in addition to the individual communication, target-audiences are people without access to the media, in Guinea Bissau, Cape Verde and Sao Tome and Principe. Under the communication plan uses the methodology, models and practices both by media professionals as health. A proximity approach and cultural mediation, previously identified key facts, are defined objectives; outlines to the Plan in concrete and its implementation methodology (target-audience and following intervention, materials to be used and key-messages and partners to mobilize) following the World Health Organisation standards.

The free- and protein-amino acids of the rumen phycomycete fungi Neocallimastix frontalis and Piromonas communis

1985 ◽  
Vol 105 (3) ◽  
pp. 523-526 ◽  
Author(s):  
P. Kemp ◽  
D. J. Jordan ◽  
C. G. Orpin
Keyword(s):  
Amino Acid ◽  
Polyacrylamide Gel Electrophoresis ◽  
Dry Weight ◽  
Amino Acid Profile ◽  
Native Proteins ◽  
Amino Acid Profiles ◽  
Protein Amino Acids ◽  
Neocallimastix Frontalis ◽  
True Protein

SUMMARYThe proteins and the amino acid profile of the rumen fungi Neocallimastix frontalis and Piromonas communis have been examined and the in vitro digestibility of the vegetative growth determined.The mean true protein content of N. frontalis was 24% of the dry weight and 30% for P. communis, which has a lower chitin content. 50% of the protein extracted from cells and examined by HPLC was in the 200000 MW range and 40% in the 50000 MW range. Polyacrylamide gel electrophoresis after treatment with sodium dodecylsulphate and 2-mercaptoethanol indicated that the native proteins were composed of smaller units in the MW range 25000–50000.The amino acid profiles of the two fungi were similar and compared favourably with those of casein and fraction 1 protein of lucerne.

scholarly journals Study on analytical characteristics of Nicotiana tabacum L., cv. Solaris biomass for potential uses in nutrition and biomethane production

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Antonella Fatica ◽  
Francesco Di Lucia ◽  
Stefano Marino ◽  
Arturo Alvino ◽  
Massimo Zuin ◽  
...  
Keyword(s):  
Amino Acid ◽  
Nicotiana Tabacum ◽  
Dry Matter ◽  
Amino Acid Profile ◽  
Biochemical Methane Potential ◽  
Total Alkaloids ◽  
Whole Plant ◽  
Methane Potential ◽  
Stem Leaf ◽  
Potential Use

AbstractIn order to limit the smoking tobacco sector crisis, a new non-GMO Nicotiana tabacum L. cv. Solaris was proposed as oil seed crop. Residues of oil extraction were successfully used in swine nutrition. The aim of this study was to explore the full potential of this innovative tobacco cultivar as multitasking feedstock non interfering with the food chain. In the triennium 2016–2018, samples from whole plant, inflorescence and stem-leaf biomass were collected in three experimental sites and analysed for chemical constituents, including fibre fractions, sugars and starch, macro-minerals and total alkaloids. The KOH soluble protein content and the amino-acid profile were also investigated as well as the biochemical methane potential. All the analyses were performed according to official methods and results were compared with values reported in literature for conventional lignocellulosic crops and agro-industry residues. The average protein content, ranging from 16.01 to 18.98 g 100 g−1 dry matter respectively for stem-leaf and whole plant samples, and their amino-acid profile are consistent with values reported for standard grass plant. These findings suggest the potential use of cv. Solaris in industrial food formulations. Moreover, considering the average content of both fibre available for fermentations (72.6% of Neutral Detergent Fibre) and oils and fats (7.92 g 100 g−1 dry matter), the whole plant biomass of cv. Solaris showed good attitude to anaerobic fermentation, confirmed by the biochemical methane potential of whole plant (168 Nm3 t−1 organic matter). Similarly, results allow to define the cv. Solaris biomass as a good quality forage apt to ensiling for its chemical composition. The low total alkaloids content of cv. Solaris, in average 0.3 g 100 g−1 dry matter, was previously reported not to affect growth performances and welfare traits of dairy heifers. These are the first results showing the multitasking potential use of cv. Solaris biomass, that could allow the recovery of tobacco cultivation know-how especially in marginal areas.

scholarly journals Amino acid profile of raw and locally processed seeds of Prosopis africana and Ricinus communis: potential antidotes to protein malnutrition

2012 ◽  
Vol 2 (4) ◽  
pp. 107 ◽  
Author(s):  
Chidi U. Igwe ◽  
Okey A. Ojiako ◽  
Ken C. Anugweje ◽  
Linus A. Nwaogu ◽  
Cosmas O. Ujowundu
Keyword(s):  
Amino Acid ◽  
Essential Amino Acid ◽  
Crude Protein ◽  
Ricinus Communis ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Protein Sources ◽  
Plant Protein Sources ◽  
Prosopis Africana ◽  
The Individual

Background: Increasing incidence of malnutrition occasioned by high incidence of hunger, worsening food situation in the world, insufficient availability and high cost of animal protein sources, has necessitated extensive research into and use of alternative plant protein sources especially underexploited leguminous seeds.Methods: Flours from raw, boiled and fermented seeds of Prosopis africana and Ricinus communis were evaluated for crude protein and amino acid (AA) profiles, and their protein qualities determined. Results: Fermentation improved the protein contents of raw seeds of P. africana and R. communis by 18.70% and 3.95% respectively. In the raw and fermented P. africana seeds, glutamate at 132.60 ± 1.30 and 182.70 ± 3.02 mg/g crude protein (mg/gcp) was the most abundant amino acid (AA), while leucine (62.80 ± 0.60 and 79.50 ± 2.01 mg/gcp) was the most concentrated essential amino acid (EAA). Aspartate (151.90 ± 2.01 and 170.10 ± 2.00 mg/gcp) and arginine (72.80 ± 2.01 and 78.60 ± 2.00 mg/gcp) were the most concentrated and abundant non-essential amino acid (NEAA) and EAA in the raw and fermented samples of R. communis respectively. The total AA concentrations (mg/gcp) of raw and fermented P. africana were 733.00 and 962.60 respectively, while those of R. communis were 823.50 and 894.10 respectively. The total EAA contents (mg/gcp) for P. africana were 311.00 (raw) and 404.50 (fermented), and for R. communis; 401.10 (raw) and 430.30 (fermented). Threonine was the limiting EAA in raw and fermented P. africana, whereas lysine was the limiting EAA in R. communis raw sample. Fermentation significantly (p<0.05) increased the individual AA compositions of P. africana and R. communis by 94% and 53% respectively, while boiling reduced these parameters significantly (p<0.05) by 47% and 82% respectively.Conclusion: P. africana and R. communis seeds are potentially important plant sources of protein and essential amino acids, and so could be of great importance in combating malnutrition and food security problems generally.Key words: Amino acid score; condiments; crude protein; fermentation; legumes; nutrition

A comprehensive model of the development of mental handicap in children treated for acute lymphoblastic leukaemia: A synthesis of the literature

2000 ◽  
Vol 24 (1) ◽  
pp. 44-58 ◽  
Author(s):  
Philippe Robaey ◽  
Patricia Dobkin ◽  
Jean-Marie Leclerc ◽  
Francine Cyr ◽  
Catherine Sauerwein ◽  
...  
Keyword(s):  
Clinical Studies ◽  
Current Knowledge ◽  
World Health Organisation ◽  
Cranial Irradiation ◽  
World Health ◽  
Complex Interactions ◽  
Biological Variables ◽  
Resilience Factors ◽  
Related Stress ◽  
The Individual

Most clinical studies addressing sequelae in children with leukaemia are not theory-driven. Nonetheless, the role of different mediating biological (e.g. cranial irradiation, chemotherapy) and psychosocial variables (e.g. family functioning) has been empirically acknowledged. In these studies, a cause-effect relationship between biological variables and cognitive deficits, sometimes complex due to multiple agents, has been hypothesised. As for the psychosocial consequences, adaptation to the cancer-related stress has been the main focus, at both the individual and family levels. In this paper, we advocate the use of a global model for the development of handicap, derived from the International Classification of Impairments, Disabilities and Handicaps (ICIDH) by the World Health Organisation. This revised model proposes that handicap is the result of complex interactions between the characteristics of a person’s impairment, disabilities, and the characteristics of the environment. At each of these levels, risk/resilience factors are defined. This means that depending on the environmental obstacles they face, persons with an impairment or a disability may or may not experience a situation creating a handicap which is no longer seen as a stable status resulting from a disease to which the individual must adapt. By reviewing animal and clinical studies, current knowledge pertaining to leukaemia sequelae are integrated into the different levels defined by the model: organic impairment, disabilities, environmental obstacles, and handicap situation. Practical implications for research, policies, and individual treatments, and comparisons with existing models, are also outlined.

Sign in / Sign up

Export Citation Format

Share Document