scholarly journals Urinary mRNA Expression of Glomerular Podocyte Markers in Glomerular Disease and Renal Transplant

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1499
Author(s):  
Silvia Armelloni ◽  
Deborah Mattinzoli ◽  
Masami Ikehata ◽  
Carlo Alfieri ◽  
Mirco Belingheri ◽  
...  
Keyword(s):  
Pilot Study ◽  
Renal Transplant ◽  
Renal Disease ◽  
Renal Biopsy ◽  
Mrna Expression ◽  
Renal Damage ◽  
Clinical Indication ◽  
Cytoskeletal Regulation ◽  
Alternative Approach ◽  
Present Pilot Study

The research of novel markers in urinary samples, for the description of renal damage, is of high interest, and several works demonstrated the value of urinary mRNA quantification for the search of events related to renal disease or affecting the outcome of transplant kidneys. In the present pilot study, a comparison of the urine mRNA expression of specific podocyte markers among patients who had undergone clinical indication to renal transplanted (RTx, n = 20) and native (N, n = 18) renal biopsy was performed. The aim of this work was to identify genes involved in podocytes signaling and cytoskeletal regulation (NPHS1, NPHS2, SYNPO, WT1, TRPC6, GRM1, and NEUROD) in respect to glomerular pathology. We considered some genes relevant for podocytes signaling and for the function of the glomerular filter applying an alternative normalization approach. Our results demonstrate the WT1 urinary mRNA increases in both groups and it is helpful for podocyte normalization. Furthermore, an increase in the expression of TRPC6 after all kinds of normalizations was observed. According to our data, WT1 normalization might be considered an alternative approach to correct the expression of urinary mRNA. In addition, our study underlines the importance of slit diaphragm proteins involved in calcium disequilibrium, such as TRPC6.

scholarly journals The modern spectrum of biopsy-proven renal disease in Chinese diabetic patients—a retrospective descriptive study

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4522 ◽  
Author(s):  
Diankun Liu ◽  
Ting Huang ◽  
Nan Chen ◽  
Gang Xu ◽  
Ping Zhang ◽  
...  
Keyword(s):  
Renal Disease ◽  
Renal Biopsy ◽  
Descriptive Study ◽  
Clinical Indication ◽  
Diabetic Patients ◽  
Tubular Necrosis ◽  
Diabetic Renal Disease ◽  
Tubulointerstitial Nephropathy ◽  
Renal Biopsies ◽  
Male Patients

Background Renal biopsies performed in diabetic patients are increasing and becoming more complex. Comprehensive data on modern spectrum of biopsy-proven renal disease in Chinese diabetic patients are lacking. Methods In a nationwide renal biopsy survey including 71,151 native biopsies from 2004 to 2014, diabetic patients were identified according to the clinical diagnosis from referral records. The clinical data were extracted from referral records and pathological reports. Results A total of 1,604 diabetic patients, including 61 patients with T1DM, were analyzed in this study. The median age is 51.39 ± 11.37 years. Male patients accounted for 58% of the population. We found that only 44.7% of diabetic patients had the isolated pathological diagnosis of diabetic nephropathy (DN), while 49.1% had non-diabetic renal disease (NDRD) alone, and 6.2% had NDRD superimposed on DN. Nephrotic syndrome (n = 824, 51.4%) was the most common clinical indication for renal biopsy. Among 887 patients with NDRD, membranous nephropathy (n = 357) was the leading diagnosis, followed by IgA nephropathy (n = 179). Hypertensive renal disease (n = 32), tubulointerstitial nephropathy (n = 27) and acute tubular necrosis (n = 16) accounted for 3.5%, 2.9%, 1.7% of the NDRD cases respectively. Nearly a half (49.2%) of patients with T1DM had NDRD. Discussion Over 55% diabetic patients with kidney disease were diagnosed as non-diabetic renal disease, among which MN and IgAN were the most common two pathological types.

Is Hepatitis C More Aggressive in Renal Transplant Patients Than in Patients With End-Stage Renal Disease?

2006 ◽  
Vol 40 (5) ◽  
pp. 444-448 ◽  
Author(s):  
Renata M. Perez ◽  
Adalgisa S. P. Ferreira ◽  
Jos?? O. Medina-Pestana ◽  
Miguel Cendoroglo-Neto ◽  
Valeria P. Lanzoni ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Renal Transplant ◽  
Renal Disease ◽  
End Stage Renal Disease ◽  
Transplant Patients ◽  
Renal Transplant Patients ◽  
Stage Renal Disease ◽  
End Stage

Mineralocorticoid Receptor Blockade Reduced Oxidative Stress in Renal Transplant Recipients: A Double-Blind, Randomized Pilot Study

2013 ◽  
Vol 37 (5) ◽  
pp. 481-490 ◽  
Author(s):  
Marcos Ojeda-Cervantes ◽  
Jonatan Barrera-Chimal ◽  
Josefina Alberú ◽  
Rosalba Pérez-Villalva ◽  
Luis Eduardo Morales-Buenrostro ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pilot Study ◽  
Renal Transplant ◽  
Mineralocorticoid Receptor ◽  
Renal Transplant Recipients ◽  
Receptor Blockade ◽  
Transplant Recipients ◽  
Double Blind ◽  
Mineralocorticoid Receptor Blockade

scholarly journals FP873IMMUNOGLOBULIN INDUCTION THERAPY IN RENAL TRANSPLANT RECIPIENTS - FIVE YEAR DATA OF A PROSPECTIVE RANDOMIZED PILOT STUDY

2015 ◽  
Vol 30 (suppl_3) ◽  
pp. iii369-iii369 ◽  
Author(s):  
Rolf Weimer ◽  
Bogumila Bartylak ◽  
Anne Staak ◽  
Fabrice Renner ◽  
Caner Suesal ◽  
...  
Keyword(s):  
Pilot Study ◽  
Renal Transplant ◽  
Induction Therapy ◽  
Renal Transplant Recipients ◽  
Transplant Recipients

scholarly journals Serum albumin fragmentation in end-stage renal disease patients – a pilot study

2009 ◽  
Vol 47 (11) ◽  
Author(s):  
Elena Donadio ◽  
Francesco Piccolomini ◽  
Veronica Dimuccio ◽  
Antonio Felicioli ◽  
Ettore Balestreri ◽  
...  
Keyword(s):  
Pilot Study ◽  
Renal Disease ◽  
Serum Albumin ◽  
End Stage Renal Disease ◽  
Stage Renal Disease ◽  
End Stage

RENAL HISTOLOGY DURING TREATMENT WITH OXAZOLIDINE-DIONES (TRIMETHADIONE, ETHADIONE, AND PARAMETHADIONE)

PEDIATRICS ◽  
1962 ◽  
Vol 30 (4) ◽  
pp. 601-607
Author(s):  
A. Bergstrand ◽  
C. G. Bergstrand ◽  
N. Engström ◽  
K. M. Herrlin
Keyword(s):  
Electron Microscopy ◽  
Nephrotic Syndrome ◽  
Renal Disease ◽  
Renal Biopsy ◽  
Clinical Symptoms ◽  
Clinical Signs ◽  
Present Knowledge ◽  
Short Report ◽  
Renal Histology ◽  
Renal Changes

Seven patients with petit mal seizures treated with trimethadione, ethadione, or paramethadione for long periods were subjected to renal biopsy at the age of 14½ 16½ years. None of the patients had clinical signs of renal disease at the time of the biopsy, but three of them had previously shown transient hematuria or proteinuria. The renal histology was studied by conventional methods and by electron microscopy, the latter method only including the glomeruli. The changes found must with the present knowledge be regarded as physiological. A short report is given of a patient with phenylketonuria and epilepsy who developed a nephrotic syndrome during treatment with tridione. No definite pathologic renal changes were demonstrated at the when the clinical symptoms had disappeared.

PERCUTANEOUS RENAL BIOPSY IN CHILDREN

PEDIATRICS ◽  
1962 ◽  
Vol 30 (2) ◽  
pp. 287-296
Author(s):  
W. F. Dodge ◽  
C. W. Daeschner ◽  
J. C. Brennan ◽  
H. S. Rosenberg ◽  
L. B. Travis ◽  
...  
Keyword(s):  
Renal Disease ◽  
Renal Biopsy ◽  
Clinical Importance ◽  
Renal Diseases ◽  
Renal Lesion ◽  
Bleeding Tendency ◽  
Percutaneous Renal Biopsy ◽  
The One ◽  
Selection Of

Since 1951, when the percutaneous renal biopsy was introduced as an adjunctive method for study of patients with renal disease, reports of some 4,000 kidney biopsies have appeared in the literature. Only about 250 of these, however, have been performed in children. A biopsy specimen containing 5 to 10 glomeruli has been reported to be adequate for interpretation and to be representative of the total renal parenchyma in 84% of the cases with diffuse renal disease. Using a biopsy technique similar to that described by Kark, we have obtained an adequate specimen in 92% of 205 kidney biopsies performed in 168 children with diffuse renal diseases. Seven deaths have been previously reported in the literature. The circumstances surrounding the death of these seven patients and of the one death that occurred in our series are described. Perirenal hematoma has had a reported incidence of 0.4%. It has been our experience, as well as that of the other investigators, that if blood boss is replaced, the patient has an otherwise uneventful course and the mass subsequently disappears. Gross hematuria has had a reported incidence of 5.2%. Microscopic hematuria, lasting for 6 to 12 hours after biopsy, has been found to be the rule rather than the exception. The complications which have occurred have been associated with bleeding, and therefore a careful history concerning bleeding tendency and a study of the clotting mechanism is essential if the risk of needle renal biopsy is to be minimized. In addition to a bleeding tendency or defect in clotting mechanism, most investigators are agreed that the presence of only one kidney or an uncooperative patient are absolute contraindications to renal biopsy. The renal biopsy is primarily, at present, an additional and most useful investigative tool in the elucidation of the pathogenesis, natural history (by serial studies) and effectiveness of specific therapy upon the various renal diseases. It is of practical clinical importance in the selection of those patients with the nephrotic syndrome in whom glucocorticoid therapy is likely to be beneficial or the patient with anuria whose renal lesion is probably reversible with time; and, as a guide to the effectiveness of therapy in patients with pyelonephritis or lupus nephritis. It is not a technique that can be recommended for general or casual use. A classification of the pathohistobogic findings of diffuse glomerulonephritis, patterned after Ellis, is presented and discussed. This classification will be used in the description and discussion of various renal diseases and systemic diseases with associated nephritis in the three subsequent papers.

Sign in / Sign up

Export Citation Format

Share Document