scholarly journals Laboratory Diagnosis of SARS-CoV-2 Pneumonia

Diagnostics ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1270
Author(s):  
Melissa R. Gitman ◽  
Maryia V. Shaban ◽  
Alberto E. Paniz-Mondolfi ◽  
Emilia M. Sordillo
Keyword(s):  
Respiratory Tract ◽  
Early Stage ◽  
Laboratory Diagnosis ◽  
Nucleic Acid Amplification ◽  
Health Economy ◽  
Diagnostic Strategies ◽  
The Public ◽  
Viral Spread ◽  
The Past ◽  
Antigen Tests

The emergence and rapid proliferation of Coronavirus Disease-2019, throughout the past year, has put an unprecedented strain on the global schema of health infrastructure and health economy. The time-sensitive agenda of identifying the virus in humans and delivering a vaccine to the public constituted an effort to flatten the statistical curve of viral spread as it grew exponentially. At the forefront of this effort was an exigency of developing rapid and accurate diagnostic strategies. These have emerged in various forms over the past year—each with strengths and weaknesses. To date, they fall into three categories: (1) those isolating and replicating viral RNA in patient samples from the respiratory tract (Nucleic Acid Amplification Tests; NAATs), (2) those detecting the presence of viral proteins (Rapid Antigen Tests; RATs) and serology-based exams identifying antibodies to the virus in whole blood and serum. The latter vary in their detection of immunoglobulins of known prevalence in early-stage and late-stage infection. With this review, we delineate the categories of testing measures developed to date, analyze the efficacy of collecting patient specimens from diverse regions of the respiratory tract, and present the up and coming technologies which have made pathogen identification easier and more accessible to the public.

Commercialising genetically engineered animal biomedical products

2008 ◽  
Vol 20 (1) ◽  
pp. 61 ◽  
Author(s):  
Eddie J. Sullivan ◽  
Jerry Pommer ◽  
James M. Robl
Keyword(s):  
Biomedical Applications ◽  
Gap Analysis ◽  
Early Stage ◽  
Genetically Engineered ◽  
The Public ◽  
Public Benefit ◽  
The Past ◽  
Cell Banking ◽  
Stage Development ◽  
Founder Animal

Research over the past two decades has increased the quality and quantity of tools available to produce genetically engineered animals. The number of potentially viable biomedical products from genetically engineered animals is increasing. However, moving from cutting-edge research to development and commercialisation of a biomedical product that is useful and wanted by the public has significant challenges. Even early stage development of genetically engineered animal applications requires consideration of many steps, including quality assurance and quality control, risk management, gap analysis, founder animal establishment, cell banking, sourcing of animals and animal-derived material, animal facilities, product collection facilities and processing facilities. These steps are complicated and expensive. Biomedical applications of genetically engineered animals have had some recent successes and many applications are well into development. As researchers consider applications for their findings, having a realistic understanding of the steps involved in the development and commercialisation of a product, produced in genetically engineered animals, is useful in determining the risk of genetic modification to the animal v. the potential public benefit of the application.

scholarly journals Update on Malaria Diagnostics and Test Utilization

2017 ◽  
Vol 55 (7) ◽  
pp. 2009-2017 ◽  
Author(s):  
Blaine A. Mathison ◽  
Bobbi S. Pritt
Keyword(s):  
Gold Standard ◽  
Acute Infection ◽  
Laboratory Diagnosis ◽  
Rapid Diagnosis ◽  
Diagnostic Methods ◽  
Nucleic Acid Amplification ◽  
Advantages And Disadvantages ◽  
Life Threatening ◽  
Antigen Tests ◽  
Malaria Test

ABSTRACT Malaria is a potentially life-threatening disease requiring rapid diagnosis and treatment. Although microscopic examination of thick and thin blood films remains the gold standard for laboratory diagnosis, rapid antigen tests and nucleic acid amplification methods may also play a useful role in detection of acute infection. This review discusses the advantages and disadvantages of the commonly used diagnostic methods and provides important practice points for optimal malaria test utilization.

scholarly journals COVID-19 Diagnostic Strategies. Part I: Nucleic Acid-Based Technologies

2021 ◽  
Vol 8 (4) ◽  
pp. 49
Author(s):  
Tina Shaffaf ◽  
Ebrahim Ghafar-Zadeh
Keyword(s):  
Mass Production ◽  
Diagnostic Tests ◽  
Cost Effective ◽  
Molecular Diagnostic ◽  
The Novel ◽  
Comprehensive Review ◽  
Diagnostic Strategies ◽  
The Public ◽  
Viral Spread

The novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has caused respiratory infection, resulting in more than two million deaths globally and hospitalizing thousands of people by March 2021. A considerable percentage of the SARS-CoV-2 positive patients are asymptomatic or pre-symptomatic carriers, facilitating the viral spread in the community by their social activities. Hence, it is critical to have access to commercialized diagnostic tests to detect the infection in the earliest stages, monitor the disease, and follow up the patients. Various technologies have been proposed to develop more promising assays and move toward the mass production of fast, reliable, cost-effective, and portable PoC diagnostic tests for COVID-19 detection. Not only COVID-19 but also many other pathogens will be able to spread and attach to human bodies in the future. These technologies enable the fast identification of high-risk individuals during future hazards to support the public in such outbreaks. This paper provides a comprehensive review of current technologies, the progress in the development of molecular diagnostic tests, and the potential strategies to facilitate innovative developments in unprecedented pandemics.

scholarly journals “The Greatest Slovak” in the Historical Memory of Modern Slovakia

2020 ◽  
Vol 162 (6) ◽  
pp. 185-193
Author(s):  
O.S. Krylova ◽  
◽  
G.V. Rokina ◽  
Keyword(s):  
Slovak Republic ◽  
Early Stage ◽  
Historical Memory ◽  
Radio Station ◽  
The Public ◽  
The Past ◽  
Stage Of Development ◽  
Public Survey ◽  
Historical Figures ◽  
Places Of Memory

The development and preservation of historical memory in the Slovak Republic were discussed using the Slovak pantheon of national heroes as an example. The study aims to show commemorative practices used in the historical development of the country. The mechanisms and tools for defining the collective historical memory of Slovaks in the modern times were considered. Some examples of the political use of memories of the past were analyzed. The title of the paper traces back to the public survey about “the greatest Slovak” arranged by the Slovak radio station and television in May 2018–October 2019. A number of examples illustrating the process of formation of historical myths and stereotypes in the collective historical memory of Slovaks over several centuries were provided. In modern Slovakia, the public is generally rejecting the old stereotypes and new myths are emerging against the background of heated discussions between politicians and historians. A simultaneous destruction of the old “places of memory” and the formation of the new ones is taking place. Furthermore, the traditional pantheon of Slovak national heroes is being updated. These processes have a considerable influence on the development of the country. In this work, special attention was paid to such historical figures of Slovakia as L. Štúr, the Founding Father of the Slovak nation, and M.R. Štefánik, the outstanding politician. The results of the television survey were analyzed in detail: the public had to choose 100 most outstanding personalities of the Slovak history, politics, and culture, as well as to determine “the greatest Slovak” among them. The latter title was finally awarded to M.R. Štefánik. It was concluded that the collective historical memory in Slovakia is at its early stage of development and that Slovaks have not fully overcome the myths and stereotypes of the past.

scholarly journals Microbiological Laboratory Diagnosis of Human Brucellosis: An Overview

Pathogens ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1623
Author(s):  
Giovanni Di Bonaventura ◽  
Silvia Angeletti ◽  
Andrea Ianni ◽  
Tommasangelo Petitti ◽  
Giovanni Gherardi
Keyword(s):  
Clinical Symptoms ◽  
Early Stage ◽  
Point Of Care ◽  
Laboratory Diagnosis ◽  
Cross Reactivity ◽  
Diagnostic Methods ◽  
Nucleic Acid Amplification ◽  
Human Brucellosis ◽  
Microbiological Analysis ◽  
Serological Tests

Brucella spp. are Gram-negative, non-motile, non-spore-forming, slow-growing, facultative intracellular bacteria causing brucellosis. Brucellosis is an endemic of specific geographic areas and, although underreported, represents the most common zoonotic infection, with an annual global incidence of 500,000 cases among humans. Humans represent an occasional host where the infection is mainly caused by B. melitensis, which is the most virulent; B. abortus; B. suis; and B. canis. A microbiological analysis is crucial to identifying human cases because clinical symptoms of human brucellosis are variable and aspecific. The laboratory diagnosis is based on three different microbiological approaches: (i) direct diagnosis by culture, (ii) indirect diagnosis by serological tests, and (iii) direct rapid diagnosis by molecular PCR-based methods. Despite the established experience with serological tests and highly sensitive nucleic acid amplification tests (NAATs), a culture is still considered the “gold standard” in the laboratory diagnosis of brucellosis due to its clinical and epidemiological relevance. Moreover, the automated BC systems now available have increased the sensitivity of BCs and shortened the time to detection of Brucella species. The main limitations of serological tests are the lack of common interpretative criteria, the suboptimal specificity due to interspecies cross-reactivity, and the low sensitivity during the early stage of disease. Despite that, serological tests remain the main diagnostic tool, especially in endemic areas because they are inexpensive, user friendly, and have high negative predictive value. Promising serological tests based on new synthetic antigens have been recently developed together with novel point-of-care tests without the need for dedicated equipment and expertise. NAATs are rapid tests that can help diagnose brucellosis in a few hours with high sensitivity and specificity. Nevertheless, the interpretation of NAAT-positive results requires attention because it may not necessarily indicate an active infection but rather a low bacterial inoculum, DNA from dead bacteria, or a patient that has recovered. Refined NAATs should be developed, and their performances should be compared with those of commercial and home-made molecular tests before being commercialized for the diagnosis of brucellosis. Here, we review and report the most common and updated microbiological diagnostic methods currently available for the laboratory diagnosis of brucellosis.

scholarly journals Effective mitigation strategy in early stage of COVID-19 pandemic in China

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Xiao-Yue Yu ◽  
Chen Xu ◽  
Hu-Wen Wang ◽  
Rui-Jie Chang ◽  
Yin-Qiao Dong ◽  
...  
Keyword(s):  
Public Health ◽  
Early Stage ◽  
Mitigation Strategy ◽  
Health Interventions ◽  
Public Health Interventions ◽  
Epidemic Control ◽  
The Public ◽  
The Past ◽  
Health Measures ◽  
Global Pandemic

Abstract In the past five months, success in control the national epidemic of coronavirus disease 2019 (COVID-19) has been witnessed in China. The implementation of public health measures accounts for the success which include different interventions in the early or later stages of the outbreak. It is clear that although not all measures were universally effective worldwide, their achievements have been significant. More solidarity is needed to deal with this global pandemic with more learning and understanding. Understanding which of the public health interventions implemented in China were effective may provide ideas for international epidemic control.

The Suffering of the Perpetrators: The Ethics of Traumatic German Historicity in Karen Bass's Young Adult World War II Novels

2014 ◽  
Vol 7 (1) ◽  
pp. 64-77
Author(s):  
Doris Wolf
Keyword(s):  
World War Ii ◽  
Young Adult ◽  
Public Sphere ◽  
Childhood Studies ◽  
Historical Narratives ◽  
World War ◽  
The Public ◽  
The Past ◽  
Adult World ◽  
The Public Sphere

This paper examines two young adult novels, Run Like Jäger (2008) and Summer of Fire (2009), by Canadian writer Karen Bass, which centre on the experiences of so-called ordinary German teenagers in World War II. Although guilt and perpetration are themes addressed in these books, their focus is primarily on the ways in which Germans suffered at the hands of the Allied forces. These books thus participate in the increasingly widespread but still controversial subject of the suffering of the perpetrators. Bringing work in childhood studies to bear on contemporary representations of German wartime suffering in the public sphere, I explore how Bass's novels, through the liminal figure of the adolescent, participate in a culture of self-victimisation that downplays guilt rather than more ethically contextualises suffering within guilt. These historical narratives are framed by contemporary narratives which centre on troubled teen protagonists who need the stories of the past for their own individualisation in the present. In their evacuation of crucial historical contexts, both Run Like Jäger and Summer of Fire support optimistic and gendered narratives of individualism that ultimately refuse complicated understandings of adolescent agency in the past or present.

The End of History Museums: What's Plan B??

2008 ◽  
Vol 30 (4) ◽  
pp. 9-27 ◽  
Author(s):  
Cary Carson
Keyword(s):  
Historic Sites ◽  
The Public ◽  
End Of History ◽  
House Museums ◽  
The Past ◽  
History Museums ◽  
Plan B ◽  
The End Of History ◽  
The Way

Abstract Are historic sites and house museums destined to go the way of Oldsmobiles and floppy disks?? Visitation has trended downwards for thirty years. Theories abound, but no one really knows why. To launch a discussion of the problem in the pages of The Public Historian, Cary Carson cautions against the pessimistic view that the past is simply passéé. Instead he offers a ““Plan B”” that takes account of the new way that learners today organize information to make history meaningful.

E-Projects Challenges in India – Role of Human Resource Management and Technology

2018 ◽  
Vol 6 (8) ◽  
pp. 263
Author(s):  
Ramnik Kaur
Keyword(s):  
Developing Countries ◽  
Public Administration ◽  
Paradigm Shift ◽  
Successful Implementation ◽  
The Public ◽  
Government Services ◽  
The Past ◽  
The Government ◽  
Traditional Approaches

E-governance is a paradigm shift over the traditional approaches in Public Administration which means rendering of government services and information to the public by using electronic means. In the past decades, service quality and responsiveness of the government towards the citizens were least important but with the approach of E-Government the government activities are now well dealt. This paper withdraws experiences from various studies from different countries and projects facing similar challenges which need to be consigned for the successful implementation of e-governance projects. Developing countries like India face poverty and illiteracy as a major obstacle in any form of development which makes it difficult for its government to provide e-services to its people conveniently and fast. It also suggests few suggestions to cope up with the challenges faced while implementing e-projects in India.

Prawne regulacje pobierania i transplantacji narządów oraz tkanek w ujęciu europejskiej konwencji bioetycznej

2016 ◽  
pp. 52-65
Author(s):  
Patryk Kołodyński ◽  
Paulina Drab
Keyword(s):  
Ex Vivo ◽  
Legal Aspects ◽  
European Convention ◽  
The Public ◽  
Additional Protocol ◽  
The Past ◽  
Reliable Source ◽  
International Legal Order ◽  
Definition Of ◽  
Source Of Information

Over the past several years, transplantology has become one of the fastest developing areas of medicine. The reason is, first and foremost, a significant improvement of the results of successful transplants. However, much controversy arouse among the public, on both medical and ethical grounds. The article presents the most important concepts and regulations relating to the collection and transplantation of organs and tissues in the context of the European Convention on Bioethics. It analyses the convention and its additional protocol. The article provides the definition of transplantation and distinguishes its types, taking into account the medical criteria for organ transplants. Moreover, authors explained the issue of organ donation ex vivo and ex mortuo. The European Convention on Human Rights and Biomedicine clearly regulates the legal aspects concerning the transplantation and related basic concepts, and therefore provides a reliable source of information about organ transplantation and tissue. This act is a part of the international legal order, which includes the established codification of bioethical standards.

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