scholarly journals Comparison of Periodontopathic Bacterial Profiles of Different Periodontal Disease Severity Using Multiplex Real-Time Polymerase Chain Reaction

Diagnostics ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 965
Author(s):  
Jin Uk Choi ◽  
Jun-Beom Lee ◽  
Kyoung-Hwa Kim ◽  
Sungtae Kim ◽  
Yang-Jo Seol ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Periodontal Disease ◽  
Real Time ◽  
Disease Severity ◽  
Sampling Methods ◽  
Study Groups ◽  
Periodontopathic Bacteria ◽  
Severe Periodontitis ◽  
Polymerase Chain ◽  
Subgingival Microbiota

Periodontopathic bacteria are known to have a pivotal role in the pathogenesis of periodontitis. The aim of the study was to quantitatively compare bacterial profile of patients with different severity of periodontal disease using samples from mouthwash and the subgingival area. Further analysis was performed to evaluate the correlation between mouthwash and two subgingival sampling methods: paperpoint and gingival retraction cord; 114 subjects enrolled in the study, and were divided equally into three groups according to disease severity. Mouthwash and subgingival sampling were conducted, and the samples were quantitatively analyzed for 11 target periodontopathic bacteria using multiplex real-time PCR. There were statistically significant differences in bacterial counts and prevalence of several species between the study groups. Mouthwash sampling showed significant correlations with two different subgingival sampling methods in regard to the detection of several bacteria (e.g., ρ = 0.793 for Porphyromonas gingivalis in severe periodontitis), implying that mouthwash sampling can reflect subgingival microbiota. However, the correlation was more prominent as disease severity increased. Although bacteria in mouthwash have potential to become a biomarker, it may be more suitable for the diagnosis of severe periodontitis, rather than early diagnosis. Further research is required for the discovery of biomarkers for early diagnosis of periodontitis.

Comparison of two rapid test kits with real time polymerase chain reaction for early diagnosis of dengue in Sri Lanka

2021 ◽  
pp. 1-9
Author(s):  
MHJD Ariyaratne ◽  
Peshala Gunasekara ◽  
Poornima Hasanthi Wajirasena ◽  
Dilini Malsha Rathnayake ◽  
Desha Dilani ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sri Lanka ◽  
Early Diagnosis ◽  
Real Time ◽  
Rapid Test ◽  
Chain Reaction ◽  
Polymerase Chain

Clinical Utility of Blood E2F3 MRNA Assay in the Early Diagnosis of Prostatic Cancer by Real - Time Polymerase Chain Reaction

2013 ◽  
Vol 64 (7-9) ◽  
pp. 655-663
Author(s):  
Mona Mohamed Zaki ◽  
Tarek Mostafa Elzayat ◽  
Manal Abdel Baky Mahmoud ◽  
Eman Saleh El Hadidi ◽  
Hala Abdel Al Ahmed ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Early Diagnosis ◽  
Real Time ◽  
Prostatic Cancer ◽  
Clinical Utility ◽  
Chain Reaction ◽  
Polymerase Chain

Application of a real time Polymerase Chain Reaction (PCR) assay for the early diagnosis of human leptospirosis in Sri Lanka

Biologicals ◽  
2016 ◽  
Vol 44 (6) ◽  
pp. 497-502 ◽  
Author(s):  
D.T.H. Denipitiya ◽  
N.V. Chandrasekharan ◽  
W. Abeyewickreme ◽  
C.M. Hartskeerl ◽  
R.A. Hartskeerl ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sri Lanka ◽  
Early Diagnosis ◽  
Real Time ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Human Leptospirosis ◽  
Polymerase Chain

scholarly journals Development of a Real-Time Polymerase Chain Reaction Assay for Quantifying Verticillium albo-atrum DNA in Resistant and Susceptible Alfalfa

2007 ◽  
Vol 97 (11) ◽  
pp. 1519-1525 ◽  
Author(s):  
R. C. Larsen ◽  
G. J. Vandemark ◽  
T. J. Hughes ◽  
C. R. Grau
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Disease Severity ◽  
Verticillium Wilt ◽  
Real Time Pcr ◽  
Severity Index ◽  
Disease Severity Index ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Pathogen Dna

A precise real-time polymerase chain reaction (PCR) assay was developed for quantifying Verticillium albo-atrum DNA. The assay was used in a repeated experiment to examine the relationship between the quantity of pathogen DNA detected in infected leaves and shoots and the severity of Verticillium wilt symptoms in several alfalfa cultivars expressing a range of disease symptoms. Plants were visually inspected for symptoms and rated using a disease severity index ranging from 1 to 5, and the quantity of pathogen DNA present in leaves and stems was determined with real-time PCR. No significant differences in pathogen DNA quantity or disease severity index were observed for experiments or for cultivar–experiment interactions. Significant differences were observed between cultivars for the quantity of pathogen DNA detected with real-time PCR and also for disease severity index ratings. In both experiments, the highly resistant check cultivar Oneida VR had significantly less pathogen DNA, and significantly lower disease severity index ratings than the resistant cultivar Samauri, the moderately resistant cultivar Vernema, and the susceptible check cultivar Saranac. In both experiments, the Spearman rank correlation between the amount of V. albo-atrum DNA detected in leaves and stems with real-time PCR and disease severity index ratings based on visual examination of symptoms was positive (>0.52) and significant (P < 0.0001). These results suggest that resistance to Verticillium wilt in alfalfa is characterized by a reduced colonization of resistant genotypes by the fungus.

scholarly journals Improved Sampling Methods for Real-Time Polymerase Chain Reaction Diagnosis of Citrus Canker from Field Samples

2004 ◽  
Vol 94 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Vessela Mavrodieva ◽  
Laurene Levy ◽  
Dean W. Gabriel
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Sampling Methods ◽  
Target Cells ◽  
Bacterial Canker ◽  
Chain Reaction ◽  
Specificity And Sensitivity ◽  
Field Samples ◽  
Citrus Bacterial Canker ◽  
Polymerase Chain

Citrus bacterial canker disease has been introduced at least three times into Florida in the last 15 years and, despite federal and state quarantine and eradication efforts, continues to spread in Florida. Accurate, fast, and reliable detection of the causal agent is of great importance. However, citrus bacterial canker is caused by at least two groups of phylogenetically distinct Xanthomonas citri strains, and there is host range variation within both groups. We developed a fast, sensitive and reliable real-time polymerase chain reaction (PCR) assay using a portable, field-hardened RAPID machine and primers designed to detect all canker-causing strains. Single-lesion sampling methods were developed that required minimal handling and allowed complete real-time PCR diagnosis in a total time of 4 h and with an apparent sensitivity of less than 10 CFU of target cells from diseased lesions. This sensitivity allowed molecular detection for the first time of X. citri in a herbarium sample from a 1912 canker outbreak. Sensitivity was improved significantly by the use of CaCO3 and Silwet L-77, and by either minimizing the amount of citrus lesion tissue sampled or by soaking or swiping but not grinding the lesions. Primer design also was of significant importance in both specificity and sensitivity.

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