scholarly journals Real-Time Polymerase Chain Reaction for Early Diagnosis of Toxoplasmosis in Stem Cell Transplant Recipients: Ready for Prime Time?

2005 ◽  
Vol 40 (1) ◽  
pp. 79-81 ◽  
Author(s):  
P. H. Chandrasekar
Keyword(s):  
Polymerase Chain Reaction ◽  
Stem Cell ◽  
Early Diagnosis ◽  
Real Time ◽  
Stem Cell Transplant ◽  
Prime Time ◽  
Cell Transplant ◽  
Transplant Recipients ◽  
Chain Reaction ◽  
Polymerase Chain

Comparison of two rapid test kits with real time polymerase chain reaction for early diagnosis of dengue in Sri Lanka

2021 ◽  
pp. 1-9
Author(s):  
MHJD Ariyaratne ◽  
Peshala Gunasekara ◽  
Poornima Hasanthi Wajirasena ◽  
Dilini Malsha Rathnayake ◽  
Desha Dilani ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sri Lanka ◽  
Early Diagnosis ◽  
Real Time ◽  
Rapid Test ◽  
Chain Reaction ◽  
Polymerase Chain

Quantitative assessment of hematopoietic chimerism after bone marrow transplantation by real-time quantitative polymerase chain reaction

Blood ◽  
2002 ◽  
Vol 99 (12) ◽  
pp. 4618-4625 ◽  
Author(s):  
Mehdi Alizadeh ◽  
Marc Bernard ◽  
Bruno Danic ◽  
Charly Dauriac ◽  
Brigitte Birebent ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Stem Cell ◽  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Assessment ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Hematopoietic Stem ◽  
Pcr Method ◽  
Polymerase Chain

We have developed a real-time quantitative polymerase chain reaction (PCR) assay using TaqMan technology (Applied Biosystems, Foster City, CA) for monitoring donor cell engraftment in allogenic hematopoietic stem cell transplant recipients. For this purpose, we selected 19 specific sequence polymorphisms belonging to 11 human biallelic loci located on 9 different chromosomes. Using a set of specially designed primers and fluorogenic probes, we evaluated the 19 markers' informativity on a panel of 126 DNA samples from 63 recipient/donor pairs. In more than 90% of these pairs, discrimination between recipient and donor genetic profile was possible. By using serial dilutions of mixed DNAs, we evaluated the linearity and sensitivity of the method. A linear correlation with rhigher than 0.98 and a sensitivity of 0.1% proved reproducible. Fluorescent-based PCR of short tandem repeats (STR-PCR) and real-time PCR chimerism assay were compared with a panel of artificial cell mixtures. The main advantage of the real-time PCR method over STR-PCR chimerism assays is the absence of PCR competition and plateau biases, and results evidenced greater sensitivity and linearity with the real-time PCR method. Furthermore, different samples can be tested in the same PCR run with a final result in fewer than 48 hours. Finally, we prospectively analyzed patients who received allografts and present 4 different clinical situations that illustrate the informativity level of our method. In conclusion, this new assay provides an accurate quantitative assessment of mixed chimerism that can be useful in guiding early implementation of additional treatments in hematopoietic stem cell transplantation.

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