scholarly journals Allelic Variants of Glutamine Synthetase and Glutamate Synthase Genes in a Collection of Durum Wheat and Association with Grain Protein Content

Diversity ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 52 ◽  
Author(s):  
Domenica Nigro ◽  
Stefania Fortunato ◽  
Stefania Giove ◽  
Giacomo Mangini ◽  
Ines Yacoubi ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Durum Wheat ◽  
Protein Content ◽  
Glutamate Synthase ◽  
Grain Protein Content ◽  
Grain Protein ◽  
Allelic Variants

Isolation and characterisation of cytosolic glutamine synthetase (GSe) genes and association with grain protein content in durum wheat

2014 ◽  
Vol 65 (1) ◽  
pp. 38 ◽  
Author(s):  
Agata Gadaleta ◽  
Domenica Nigro ◽  
Ilaria Marcotuli ◽  
Angelica Giancaspro ◽  
Stefania L. Giove ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Durum Wheat ◽  
Protein Content ◽  
Quantitative Trait ◽  
Glutamate Synthase ◽  
Grain Protein Content ◽  
Grain Protein ◽  
Quantitative Real Time Pcr ◽  
Concerted Action ◽  
Cytosolic Glutamine Synthetase

Glutamine synthetase (GS) enzyme (EC 6.3.1.2) plays a central role in assimilating ammonia produced in the leaf from metabolic processes, spanning from assimilation to transamination reactions and catabolic processes. GS is located in both cytoplasm (GS1, GSe and GSr) and plastids (GS2) of plant cells. Glutamine and glutamate, produced by the concerted action of GS and glutamate synthase, are then transported from the leaf to the developing sinks or grain in wheat. The goal of the present study was to characterise GSe genes and to assess the linkage with grain protein content, an important quantitative trait controlled by multiple genes. Here, we report the isolation of the complete cytosolic GS gene sequences of the durum wheat cvv. ‘Ciccio’ and ‘Svevo’ (characterised by low and high protein content, respectively). GSe-A4 located on 4A chromosome comprises 12 exons separated by 11 introns, while the GSe-B4 gene on 4B chromosome comprises 11 exons separated by 10 introns. Quantitative real-time PCR indicated different expression levels of GSe-A4 and GSe-B4 genes in the two wheat cvv. ‘Ciccio’ and ‘Svevo’. The two GSe genes were significantly associated to quantitative trait loci for grain protein content.

scholarly journals Glutamine synthetase in Durum Wheat: Genotypic Variation and Relationship with Grain Protein Content

2016 ◽  
Vol 7 ◽  
Author(s):  
Domenica Nigro ◽  
Stefania Fortunato ◽  
Stefania L. Giove ◽  
Annalisa Paradiso ◽  
Yong Q. Gu ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Durum Wheat ◽  
Protein Content ◽  
Genotypic Variation ◽  
Grain Protein Content ◽  
Grain Protein

The glutamine synthetase (GS2) genes in relation to grain protein content of durum wheat

2011 ◽  
Vol 11 (4) ◽  
pp. 665-670 ◽  
Author(s):  
Agata Gadaleta ◽  
Domenica Nigro ◽  
Angelica Giancaspro ◽  
Antonio Blanco
Keyword(s):  
Glutamine Synthetase ◽  
Durum Wheat ◽  
Protein Content ◽  
Grain Protein Content ◽  
Grain Protein

scholarly journals Nitrogen Metabolism at Tillering Stage Differently Affects the Grain Yield and Grain Protein Content in Two Durum Wheat Cultivars

Diversity ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 186
Author(s):  
Fortunato ◽  
Nigro ◽  
Paradiso ◽  
Cucci ◽  
Lacolla ◽  
...  
Keyword(s):  
Amino Acids ◽  
Grain Yield ◽  
Durum Wheat ◽  
Protein Content ◽  
Grain Protein Content ◽  
Cultivar Differences ◽  
Grain Protein ◽  
Wheat Cultivars ◽  
Nitrogen Use ◽  
Nitrogen Abundance

Soil nitrogen abundance, as well as nitrogen use efficiency (NUE), significantly affect the crop yield and grain protein content (GPC). Depending on the genotype, a negative correlation between the yield and GPC can occur. The aim of the study was to assess the agronomic performance, and to explore physiological pathways for the efficient use of N fertilizer for two durum wheat cultivars, “Aureo” and “Vespucci”. After fertilization, the nitrogen content and values of some of the agronomic parameters and yield-related traits increased in both cultivars; nevertheless, a simultaneous rise in both the yield and GPC occurred only in Aureo. The biochemical parameters, analyzed at tillering, confirm the genotypic specificity of nitrogen use. In Vespucci’s roots, the nitrogen supply did not affect the nitrate reductase (NR), but greatly increased the amino acids and proteins, suggesting that ammonium is preferentially assimilated. In Aureo, nitrate is in part assimilated by the roots, as suggested by the ammonium increase and NR enhancement. In the leaves of both cultivars, organic nitrogen significantly increased after fertilization; however, the rise in amino acids, as well as in NR activity, was higher in Aureo than in Vespucci. These results indicate that the different nitrogen use, and in particular the diverse NR behavior, at tillering, are in part responsible of the cultivar differences in grain yield and GPC.

scholarly journals Seeding rate and genotype effects on agronomic performance and grain protein content of durum wheat (Triticum turgidum L. Durum) in south-eastern Ethiopia

2013 ◽  
Vol 13 (58) ◽  
pp. 7693-7710
Author(s):  
Haile D ◽  
◽  
R Nigussie-Dechassa ◽  
W Abdo ◽  
F Girma ◽  
...  
Keyword(s):  
Grain Yield ◽  
Durum Wheat ◽  
Protein Content ◽  
Agronomic Traits ◽  
Grain Protein Content ◽  
Grain Protein ◽  
Agronomic Performance ◽  
Seeding Rate ◽  
Eastern Ethiopia ◽  
Number Of Seeds

The use of optimum seeding rate for the genotype may enhance productivity and grain protein content of durum wheat. Therefore, an experiment was conducted at two locations in south-eastern Ethiopia during the main cropping season of 2008 with the objective of elucidating the effects of seeding rate and genotype on agronomic performance and grain protein content of the crop. The experiment consisted of factorial arrangements of four improved durum wheat genotypes and five seeding rates, which were laid out as a randomized complete block design with three replicates. Seeding rates significantly influenced agronomic performances including number of fertile spikes m-2, plant height, number of seeds spike-1, and grain yield. Number of fertile spikes m-2 was increased proportionally with the seeding rate and the highest number (382 spikes m-2) was recorded in the highest seeding rate of 200kg ha-1. Inversely, the highest number of kernels spike-1 (29.8) was at the seeding rate of 100 kg ha-1. The highest grain yield (4341 kg ha-1) was obtained in response to seeding rate of 175 kg ha-1, which was in statistical parity with the yield obtained at the seeding rate of 150 kg ha-1. However, grain protein content was not influenced by the seeding rates. There were significant (P ≤ 0.05) variations among the genotypes for all the agronomic traits measured. The largest number of fertile spikes m-2 was recorded for the genotypes Oda (360 spikes m-2) and Bakalcha (345 spikes m-2). Genotype Illani produced the longest spike (6.9 cm). Oda and Illani produced the highest numbers of seeds spike-1, 38.8 and 36.9, respectively. The number of fertile spikes m-2, number of seeds spike-2 and kernels weight significantly contributed grain yield. The genotypes had exhibited less variation for grain protein content. Except for grain yield and harvest index, seeding rate x genotype interaction had no significant effect on other agronomic traits. Highest grain yields of 4938 kg ha-1 and 4774 kg ha1 were obtained from genotypes Ejersa and Bakalcha when sown at the seeding rate of 150 kg ha-1 and 175 kg ha-1, respectively. Grain protein response was significantly influenced by the interaction effect in which genotype Oda had the highest (12.9%) and lowest (10.5%) protein contents at the highest (200 kg ha-1) and lowest (100 kg ha-1) seeding rates, respectively.

Tos17 insertion in NADH-dependent glutamate synthase genes leads to an increase in grain protein content in rice

2018 ◽  
Vol 84 ◽  
pp. 38-43 ◽  
Author(s):  
Fumi Imagawa ◽  
Haruka Minagawa ◽  
Yosuke Nakayama ◽  
Keiichi Kanno ◽  
Toshihiko Hayakawa ◽  
...  
Keyword(s):  
Protein Content ◽  
Glutamate Synthase ◽  
Grain Protein Content ◽  
Grain Protein

Candidate genes and genome-wide association study of grain protein content and protein deviation in durum wheat

Planta ◽  
2019 ◽  
Vol 249 (4) ◽  
pp. 1157-1175 ◽  
Author(s):  
D. Nigro ◽  
A. Gadaleta ◽  
G. Mangini ◽  
P. Colasuonno ◽  
I. Marcotuli ◽  
...  
Keyword(s):  
Association Study ◽  
Durum Wheat ◽  
Protein Content ◽  
Candidate Genes ◽  
Genome Wide Association Study ◽  
Grain Protein Content ◽  
Genome Wide Association ◽  
Grain Protein ◽  
Genome Wide
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