scholarly journals Determination of Genetic Variations of Toll-Like Receptor (TLR) 2, 4, and 6 with Next-Generation Sequencing in Native Cattle Breeds of Anatolia and Holstein Friesian

Diversity ◽  
2016 ◽  
Vol 8 (4) ◽  
pp. 23 ◽  
Author(s):  
Nuket Bilgen ◽  
Bengi Cinar Kul ◽  
Victoria Offord ◽  
Dirk Werling ◽  
Okan Ertugrul
Keyword(s):  
Next Generation Sequencing ◽  
Genetic Variations ◽  
Toll Like Receptor ◽  
Next Generation ◽  
Cattle Breeds ◽  
Holstein Friesian ◽  
Native Cattle ◽  
Generation Sequencing

scholarly journals Determination of Genetic Variations of Toll-like Receptor (TLR) 2, 4 and 6 with Next Generation Sequencing in Native Cattle Breeds of Anatolia and Holstein Friesian

2016 ◽  
Author(s):  
Nuket Bilgen ◽  
Bengi Cinar Kul ◽  
Victoria Offord ◽  
Dirk Werling ◽  
Okan Ertugrul
Keyword(s):  
Disease Resistance ◽  
Next Generation Sequencing ◽  
Bacterial Infections ◽  
Bovine Genome ◽  
Genetic Selection ◽  
Next Generation ◽  
Indigenous Cattle ◽  
Cattle Breeds ◽  
Holstein Friesian ◽  
Generation Sequencing

In recent years, the focus of disease resistance and susceptibility studies in cattle have been on determining patterns in the innate immune response of key proteins, such as Toll-like receptors (TLR). In the bovine genome, there are 10 TLR family members and, of these, TLR2, TLR4 and TLR6 are specialized in recognition of bacterial ligands. Indigenous cattle breeds of Anatolia have been reported to show fewer signs of clinical bacterial infections, such as bovine tuberculosis and mastitis, and it is hypothesized that this might be due to a less stringent genetic selection during breeding. In contrast, Holstein-Friesian cattle have been under strong selection for milk production, which may have resulted in greater susceptibility to diseases. To test this hypothesis, we have compared the TLR2, TLR4 and TLR6 genes of Anatolian Black (AB), East Anatolian Red (EAR), South Anatolian Red (SAR), Turkish Grey (TG), and Holstein (HOL) cattle using Next Generation Sequencing. The SAR breed had the most variations overall, followed by EAR, AB, TG and HOL. TG had the most variations for TLR2 whereas SAR had the most variations in TLR4 and TLR6. We compared these variants with those associated with disease and susceptibility traits. We used exon variants to construct haplotypes, investigated shared haplotypes within breeds and determined candidate haplotypes for disease resistance phenotype in Anatolian cattle breeds.

A Benchmark of Structural Variant Analysis Tools for Next Generation Sequencing Data

2013 ◽  
Vol 2 (4) ◽  
pp. 86-98
Author(s):  
Chatzinikolaou Panagiotis ◽  
Makris Christos ◽  
Dimitrios Vlachakis ◽  
Sophia Kossida
Keyword(s):  
Next Generation Sequencing ◽  
Level Structure ◽  
Atomic Level ◽  
Protein Sequencing ◽  
Next Generation Sequencing Data ◽  
Next Generation ◽  
Sequencing Data ◽  
Variant Analysis ◽  
Generation Sequencing

In language of genetics and biochemistry, sequencing is the determination of an unbranched biopolymer's primary structure. A sequence is a symbolic linear depiction, result of sequencing. This sequence is a succinct summary of the most of the sequenced molecule's atomic-level structure. (Most known is DNA-sequencing, RNA-sequencing, Protein-sequencing and Next-Generation-sequencing)

Next generation sequencing: implications in personalized medicine and pharmacogenomics

2016 ◽  
Vol 12 (6) ◽  
pp. 1818-1830 ◽  
Author(s):  
Bahareh Rabbani ◽  
Hirofumi Nakaoka ◽  
Shahin Akhondzadeh ◽  
Mustafa Tekin ◽  
Nejat Mahdieh
Keyword(s):  
Next Generation Sequencing ◽  
Personalized Medicine ◽  
Genetic Variations ◽  
Next Generation ◽  
Health And Disease ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing

A breakthrough in next generation sequencing (NGS) in the last decade provided an unprecedented opportunity to investigate genetic variations in humans and their roles in health and disease.

scholarly journals Next-Generation Sequencing of HIV-1 RNA Genomes: Determination of Error Rates and Minimizing Artificial Recombination

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e74249 ◽  
Author(s):  
Francesca Di Giallonardo ◽  
Osvaldo Zagordi ◽  
Yannick Duport ◽  
Christine Leemann ◽  
Beda Joos ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Error Rates ◽  
Next Generation ◽  
Generation Sequencing ◽  
Hiv 1

scholarly journals Gestational Psittacosis With Secondary Hemophagocytic Syndrome: A Case Report and Literature Review

2021 ◽  
Vol 8 ◽  
Author(s):  
Li Sun ◽  
Pulin Li ◽  
Bo Pang ◽  
Peipei Wu ◽  
Ran Wang
Keyword(s):  
Bone Marrow ◽  
Next Generation Sequencing ◽  
Hemophagocytic Syndrome ◽  
Nk Cell ◽  
Cell Activity ◽  
Lung Infection ◽  
Next Generation ◽  
Nk Cell Activity ◽  
Generation Sequencing

Gestational psittacosis and hemophagocytic syndrome (HPS) are rare clinical diseases. In this article, a case of gestational psittacosis concomitant with secondary HPS was reported. An analysis was performed on the clinical characteristics, signs, laboratory findings, progression, diagnosis, and treatment of a patient with gestational psittacosis concomitant with secondary HPS. Besides, the literature with respect to this disease was reviewed. This patient was definitively diagnosed through metagenomic next-generation sequencing techniques, bone marrow puncture and smear examination, and the determination of sCD25 level and natural killer (NK) cell activity. Anti-infectives such as doxycycline and etoposide combined with hormone chemotherapy achieved significant improvement in cough and expectoration, a return to normal temperature, and a significant improvement in oxygenation index. In addition, chest computed tomography revealed obvious absorption of lung lesions and a return of NK cell activity and sCD25 levels to normal ranges. Chlamydia psittaci pneumonia requires a clear determination of etiology, while HPS requires bone marrow puncture and smear examination, together with the determination of sCD25 level and NK cell activity in the blood. The findings of this study suggest that metagenomic next-generation sequencing is an effective instrument in clearly identifying pathogens that cause lung infection. Clinicians should consider atypical pathogens of lung infection in patients with poor response to empirical anti-infectives, and strive to design an effective treatment strategy as per an accurate diagnosis based on the etiology. As for patients suffering from long-term high fever and poor temperature control after broad-spectrum antibiotic treatment, non-infectious fever should be taken into account. A rapid and clear diagnosis would significantly improve patient prognosis.

Abstract 3585: Determination of gene amplifications with a next-generation sequencing cancer panel

2017 ◽  
Author(s):  
Ina L. Deras ◽  
Aaron Wise ◽  
Chen Zhao ◽  
Christine Glidewell-Kenney ◽  
Phillip Le ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Next Generation ◽  
Generation Sequencing ◽  
Cancer Panel

The use of next-generation sequencing for the determination of rare blood group genotypes

2017 ◽  
Vol 29 (3) ◽  
pp. 162-168 ◽  
Author(s):  
M. A. Jakobsen ◽  
C. Dellgren ◽  
C. Sheppard ◽  
M. Yazer ◽  
U. Sprogøe
Keyword(s):  
Next Generation Sequencing ◽  
Blood Group ◽  
Next Generation ◽  
Generation Sequencing
Sign in / Sign up

Export Citation Format

Share Document