scholarly journals Building a Plant DNA Barcode Reference Library for a Diverse Tropical Flora: An Example from Queensland, Australia

Diversity ◽  
2016 ◽  
Vol 8 (1) ◽  
pp. 5 ◽  
Author(s):  
Craig Costion ◽  
Andrew Lowe ◽  
Maurizio Rossetto ◽  
Robert Kooyman ◽  
Martin Breed ◽  
...  
Keyword(s):  
Dna Barcode ◽  
Reference Library ◽  
Plant Dna ◽  
Tropical Flora

Accelerating plant DNA barcode reference library construction using herbarium specimens: improved experimental techniques

2015 ◽  
Vol 15 (6) ◽  
pp. 1366-1374 ◽  
Author(s):  
Chao Xu ◽  
Wenpan Dong ◽  
Shuo Shi ◽  
Tao Cheng ◽  
Changhao Li ◽  
...  
Keyword(s):  
Dna Barcode ◽  
Experimental Techniques ◽  
Herbarium Specimens ◽  
Reference Library ◽  
Library Construction ◽  
Plant Dna

scholarly journals A DNA barcode reference library of the French Polynesian shore fishes

2019 ◽  
Author(s):  
Erwan Delrieu-Trottin ◽  
Jeffrey T. Williams ◽  
Diane Pitassy ◽  
Amy Driskell ◽  
Nicolas Hubert ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Biological Diversity ◽  
Dna Barcode ◽  
Reef Fishes ◽  
French Polynesia ◽  
Morphological Characters ◽  
Reference Library ◽  
Society Islands ◽  
Extensive Collection ◽  
I Gene

AbstractThe emergence of DNA barcoding and metabarcoding opened new ways to study biological diversity, however, the completion of DNA barcode libraries is fundamental for such approaches to succeed. This dataset is a DNA barcode reference library (fragment of Cytochrome Oxydase I gene) for 2,190 specimens representing at least 540 species of shore fishes collected over 10 years at 154 sites across the four volcanic archipelagos of French Polynesia; the Austral, Gambier, Marquesas and Society Islands, a 5,000,000 km2area. At present, 65% of the known shore fish species of these archipelagoes possess a DNA barcode associated with preserved, photographed, tissue sampled and cataloged specimens, and extensive collection locality data. This dataset represents one of the most comprehensive DNA barcoding efforts for a vertebrate fauna to date. Considering the challenges associated with the conservation of coral reef fishes and the difficulties of accurately identifying species using morphological characters, this publicly available library is expected to be helpful for both authorities and academics in various fields.

Strengthening the DNA barcode reference library for South African estuarine macrofauna

2021 ◽  
Vol 43 (1) ◽  
pp. 141-145
Author(s):  
C Fagg ◽  
NL Phair ◽  
L Claassens ◽  
RSK Barnes ◽  
S von der Heyden
Keyword(s):  
South African ◽  
Dna Barcode ◽  
Reference Library

scholarly journals BOKP: A DNA Barcode Reference Library for Monitoring Herbal Drugs in the Korean Pharmacopeia

2017 ◽  
Vol 8 ◽  
Author(s):  
Jinxin Liu ◽  
Linchun Shi ◽  
Jingyuan Song ◽  
Wei Sun ◽  
Jianping Han ◽  
...  
Keyword(s):  
Dna Barcode ◽  
Herbal Drugs ◽  
Reference Library

scholarly journals Barcode DNA Tanaman Leilem (Clerodendrum minahassae L.) Berdasarkan Gen matK

Jurnal MIPA ◽  
2014 ◽  
Vol 3 (2) ◽  
pp. 108
Author(s):  
Cindy Kalangi ◽  
Vanda S. Kamu ◽  
Maureen Kumaunang
Keyword(s):  
Dna Barcode ◽  
Plant Dna ◽  
Pcr Product ◽  
Barcode Dna ◽  
Two Samples ◽  
Matk Gene ◽  
Plant Chloroplast ◽  
Reverse Primer ◽  
Total Dna ◽  
Pcr Method

Gen matK merupakan gen pengkode protein maturaseK yang terdapat pada kloroplas tumbuhan. Penelitian ini bertujuan untuk menentukan urutan nukleotida dari barcode DNA tanaman leilem (Clerodendrum minahassae L.) berdasarkan gen matK. Prosedur penelitian yang dilakukan meliputi: isolasi DNA total tanaman leilem, amplifikasi gen matK melalui PCR, sekuensing hasil PCR, serta penentuan barcode DNA leilem. Isolasi DNA total dari tanaman leilem telah dilakukan berdasarkan prosedur manual dari InnuPrep Plant DNA Kit yang dimodifikasi dengan menghasilkan larutan berwarna hijau kekuningan yang menunjukkan adanya klorofil yang larut. Gen matK parsial telah diisolasi dengan metode Polymerase Chain Reaction (PCR) menggunakan Primer Forward matK-1RKIM-f dan Primer Reverse matK-3FKIM-r. Analisis urutan nukleotida matK menghasilkan fragmen berukuran 843 pb. Kedua urutan nukleotida matK dari sampel tanaman leilem yang berasal dari Kauditan dan Tomohon menunjukkan hasil barcode DNA yang sama.MaturaseK is a protein encoded by matK gene which is located in plant chloroplast. The aim of this research was to determine the DNA barcode of leilem plant (Clerodendrum minahassae L.) based on matK nucleotides sequence. This research was done by isolating total DNA of leilem, amplified matK gene by PCR, sequencing the PCR product, and determined the DNA barcode of leilem. Total DNA of leilem plant was isolated by using the modified procedure from InnuPrep Plant DNA Kit. The DNA isolation resulted a green-yellowish solution which shows dissolved chlorophyll. Partial matK gene was amplified using PCR method with matK-1RKIM-f as forward primer and matK-3FKIM-r as reverse primer. Amplification by PCR resulted a 843 bp DNA fragment of matK. Both nucleotide sequences of matK from two samples of leilem plant taken from Kauditan and Tomohon showed the same DNA barcode.

scholarly journals A DNA barcode reference library for the superorder Peracarida (Crustacea) from the Southern European Atlantic coast

2016 ◽  
Vol 3 ◽  
Author(s):  
Vieira Pedro ◽  
Gomes Nuno ◽  
Lobo Jorge ◽  
Borges Luisa ◽  
Queiroga Henrique ◽  
...  
Keyword(s):  
Atlantic Coast ◽  
Dna Barcode ◽  
Reference Library

scholarly journals Towards an Iberian DNA barcode reference library of freshwater macroinvertebrates and fishes

Limnetica ◽  
2020 ◽  
Vol 39 (1) ◽  
pp. 73-92
Author(s):  
Cesc Múrria ◽  
Simona Somma ◽  
Owen S. Wangensteen ◽  
Miquel A. Arnedo ◽  
Narcís Prat
Keyword(s):  
Dna Barcode ◽  
Reference Library ◽  
Freshwater Macroinvertebrates

scholarly journals DNA barcode reference library for Iberian butterflies enables a continental-scale preview of potential cryptic diversity

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Vlad Dincă ◽  
Sergio Montagud ◽  
Gerard Talavera ◽  
Juan Hernández-Roldán ◽  
Miguel L. Munguira ◽  
...  
Keyword(s):  
Dna Barcode ◽  
Cryptic Diversity ◽  
Reference Library ◽  
Continental Scale

The nuclear internal transcribed spacer (ITS2) as a practical plant DNA barcode for herbal medicines

2016 ◽  
Vol 3 (3) ◽  
pp. 94-100 ◽  
Author(s):  
Claire-Iphanise Michel ◽  
Rachel S. Meyer ◽  
Yanille Taveras ◽  
Jeanmaire Molina
Keyword(s):  
Internal Transcribed Spacer ◽  
Dna Barcode ◽  
Herbal Medicines ◽  
Plant Dna

scholarly journals Calibrating the taxonomy of a megadiverse insect family: 3000 DNA barcodes from geometrid type specimens (Lepidoptera, Geometridae)

Genome ◽  
2016 ◽  
Vol 59 (9) ◽  
pp. 671-684 ◽  
Author(s):  
Axel Hausmann ◽  
Scott E. Miller ◽  
Jeremy D. Holloway ◽  
Jeremy R. deWaard ◽  
David Pollock ◽  
...  
Keyword(s):  
18Th Century ◽  
Dna Barcode ◽  
Data Systems ◽  
Dna Barcodes ◽  
Reference Library ◽  
Type Specimens ◽  
Voucher Specimen ◽  
Tropical Regions ◽  
Taxonomic Assignments ◽  
Insect Family

It is essential that any DNA barcode reference library be based upon correctly identified specimens. The Barcode of Life Data Systems (BOLD) requires information such as images, geo-referencing, and details on the museum holding the voucher specimen for each barcode record to aid recognition of potential misidentifications. Nevertheless, there are misidentifications and incomplete identifications (e.g., to a genus or family) on BOLD, mainly for species from tropical regions. Unfortunately, experts are often unavailable to correct taxonomic assignments due to time constraints and the lack of specialists for many groups and regions. However, considerable progress could be made if barcode records were available for all type specimens. As a result of recent improvements in analytical protocols, it is now possible to recover barcode sequences from museum specimens that date to the start of taxonomic work in the 18th century. The present study discusses success in the recovery of DNA barcode sequences from 2805 type specimens of geometrid moths which represent 1965 species, corresponding to about 9% of the 23 000 described species in this family worldwide and including 1875 taxa represented by name-bearing types. Sequencing success was high (73% of specimens), even for specimens that were more than a century old. Several case studies are discussed to show the efficiency, reliability, and sustainability of this approach.

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