scholarly journals A DNA barcode reference library of the French Polynesian shore fishes

2019 ◽  
Author(s):  
Erwan Delrieu-Trottin ◽  
Jeffrey T. Williams ◽  
Diane Pitassy ◽  
Amy Driskell ◽  
Nicolas Hubert ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Biological Diversity ◽  
Dna Barcode ◽  
Reef Fishes ◽  
French Polynesia ◽  
Morphological Characters ◽  
Reference Library ◽  
Society Islands ◽  
Extensive Collection ◽  
I Gene

AbstractThe emergence of DNA barcoding and metabarcoding opened new ways to study biological diversity, however, the completion of DNA barcode libraries is fundamental for such approaches to succeed. This dataset is a DNA barcode reference library (fragment of Cytochrome Oxydase I gene) for 2,190 specimens representing at least 540 species of shore fishes collected over 10 years at 154 sites across the four volcanic archipelagos of French Polynesia; the Austral, Gambier, Marquesas and Society Islands, a 5,000,000 km2area. At present, 65% of the known shore fish species of these archipelagoes possess a DNA barcode associated with preserved, photographed, tissue sampled and cataloged specimens, and extensive collection locality data. This dataset represents one of the most comprehensive DNA barcoding efforts for a vertebrate fauna to date. Considering the challenges associated with the conservation of coral reef fishes and the difficulties of accurately identifying species using morphological characters, this publicly available library is expected to be helpful for both authorities and academics in various fields.

scholarly journals Establishing the first DNA barcode reference library for freshwater decapod species (Crustacea: Decapoda) in Republic of North Macedonia

2021 ◽  
Vol 4 ◽  
Author(s):  
Valentina Slavevska Stamenkovic ◽  
Jelena Hinic ◽  
Michal Grabowski ◽  
Tomasz Mamos ◽  
Leona Lovrenčić ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Dna Barcode ◽  
Morphological Characters ◽  
Freshwater Ecosystems ◽  
Aquatic Biota ◽  
Reference Library ◽  
Irreversible Loss ◽  
The Republic ◽  
Different Parts ◽  
Comprehensive Study

The freshwater ecosystems in the Republic of North Macedonia are considered as biodiversity hotspot on the European level since they provide diverse habitats that correspond with the complex of ecological preferences that many species require. This specially applies to the freshwater crustaceans that, unfortunately, have never been in the focus of a continuous research. In R. North Macedonia, freshwater crustaceans usually inhabit ecosystems exposed to negative anthropogenic impact. Thus, some species may become extinct presenting an irreversible loss for the Macedonian natural heritage. Although DNA barcoding, as a highly effective tool for fast species detection, is already a routine protocol in many taxonomical studies all over the world, there is still no official national DNA barcoding initiative in Republic of North Macedonia. This study employs DNA barcoding based on the ca. 650-bp long standard fragment of the mt COI gene of Astacus astacus, Austropotamobius torrentium, Potamon fluviatile and Potamon ibericum previously identified based on morphological characters, collected from different parts in R. North Macedonia. The ability of the DNA barcoding to rapidly identify all species has been proven. Our research presents the first comprehensive study that employs DNA barcoding as a molecular tool in decapod taxonomy in Republic of North Macedonia, giving the first attempt to establish DNA barcode reference library for freshwater decapod species in the country. We hope that further application of this approach will lead to the construction of DNA barcode reference library for different aquatic biota in the country. Such a library will find purpose in effective and modern bioassessment protocols as well as in phylogenetic research detecting interpopulation genetic variability.

Spirocamallanus species of French Polynesian coral reef fishes

1997 ◽  
Vol 75 (8) ◽  
pp. 1270-1279 ◽  
Author(s):  
Mark C. Rigby ◽  
Martin L. Adamson
Keyword(s):  
Coral Reef ◽  
Ecological Factors ◽  
Buccal Capsule ◽  
Reef Fishes ◽  
French Polynesia ◽  
New Host ◽  
Society Islands ◽  
Single Clade ◽  
Taxonomic Importance ◽  
New Host Records

Spirocamallanus monotaxis is redescribed from Monotaxis grandoculis (Lethrinidae) and reported from 10 other species of coral reef associated fishes from both the Society Islands and the Tuamotu Islands. This represents a new locality for S. monotaxis and 10 new host records. Spirocamallanus colei n.sp. is described from Acanthurus achilles (Acanthuridae) and from Acanthurus guttatus, Acanthurus lineatus, Acanthurus triostegus, and Zebrasoma scopas from both the Society Islands and the Tuamotu Islands. Spirocamallanus chaimha n.sp. is described from Ctenochaetus striatus and Acanthurus olivaceous (Acanthuridae) from Moorea in the Society Islands. The number of buccal capsule ridges in Spirocamallanus varies and minor differences have no taxonomic importance. Marine Spirocamallanus species appear to belong to a single clade characterized by 3 preanal papillae and 5 postanal papillae. This clade may be subdivided on the basis of the shape of the female tail. In French Polynesia, ecological factors as opposed to phylogenetic factors appear to determine host specificity for Spirocamallanus. A new system of reporting the positions of the caudal papillae, based on the position of the papillae relative to the length of the alae, is used.

An example of problems associated with DNA barcoding in tardigrades: a novel method for obtaining voucher specimens

Zootaxa ◽  
2011 ◽  
Vol 3104 (1) ◽  
pp. 42 ◽  
Author(s):  
MICHELE CESARI ◽  
ILARIA GIOVANNINI ◽  
ROBERTO BERTOLANI ◽  
LORENA REBECCHI
Keyword(s):  
Dna Barcoding ◽  
Dna Barcode ◽  
Intraspecific Variability ◽  
Original Description ◽  
Morphological Characters ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Type Locality ◽  
Moss Sample ◽  
Voucher Specimens

We have in recent papers revealed that an integrative taxonomy approach helps to solve taxonomic problems in tardigrades. However, whole tardigrades are required for DNA work, which leaves no hologenophore voucher specimens with adult morphology. Using a novel methodology for the Tardigrada, we introduce the practice of collecting high quality maximum magnification light microscopy images of recently thawed animals to act as hologenophore voucher specimens of animals later used for DNA barcode sequencing. Within the framework of a DNA barcoding project on tardigrades, we collected a moss sample from the type locality of Macrobiotus terminalis Bertolani & Rebecchi, 1993 (Castelsantangelo, Central Apennines, Italy), a species of the “Macrobiotus hufelandi group”. Within the moss sample we found several animals and eggs with a morphology that corresponded to the original description of M. terminalis, while others were attributable to Macrobiotus macrocalix Bertolani & Rebecchi, 1993. In this study, molecular (cox1 mtDNA) analyses demonstrated no intraspecific variability in M. terminalis from the type locality but very large interspecific differences when compared with M. macrocalix and GenBank data for other species within the M. “hufelandi group”. There was also a large difference between our M. terminalis sequences and the GenBank data of a specimen attributed to the same species. The GenBank sequence originated from a population in the Northern Apennines, whose morphology appeared to be like that of the specimens of the locus typicus. This confirmed the importance in utilising material from the type locality for linking molecular data to the species’ morphological characters. Our paper underlines the importance of an integrative taxonomy in species diagnoses and demonstrates a scenario where morphological observations alone are not always sufficient. Lastly, this work adds reliable information to the sequence reference library that provides a useful building block for further studies on similar and related tardigrade taxa.

scholarly journals A DNA barcode-based survey of terrestrial arthropods in the Society Islands of French Polynesia: host diversity within the SymbioCode Project

2017 ◽  
Author(s):  
Thibault Ramage ◽  
Patricia Martins-Simoes ◽  
Gladys Mialdea ◽  
Roland Allemand ◽  
Anne Duplouy ◽  
...  
Keyword(s):  
Molecular Diversity ◽  
Genetic Material ◽  
Dna Barcode ◽  
French Polynesia ◽  
Molecular Data ◽  
Species Boundaries ◽  
Bacterial Symbionts ◽  
Society Islands ◽  
Terrestrial Arthropods ◽  
Natural Laboratory

We report here on the taxonomic and molecular diversity of 10 929 terrestrial arthropod specimens, collected on four islands of the Society Archipelago, French Polynesia. The survey was part of the ‘SymbioCode Project’ that aims to establish the Society Islands as a natural laboratory in which to investigate the flux of bacterial symbionts (e.g., Wolbachia) and other genetic material among branches of the arthropod tree. The sample includes an estimated 1127 species, of which 1098 included at least one DNA-barcoded specimen and 29 were identified to species level using morphological traits only. Species counts based on molecular data emphasize that some groups have been understudied in this region and deserve more focused taxonomic effort, notably Diptera, Lepidoptera and Hymenoptera. Some taxa that were also subjected to morphological scrutiny reveal a consistent match between DNA and morphology-based species boundaries in 90% of the cases, with a larger than expected genetic diversity in the remaining 10%. Many species from this sample are new to this region or are undescribed. Some are under description, but many await inspection by motivated experts, who can use the online images or request access to ethanol-stored specimens.

scholarly journals DNA barcoding of commercially important reef fishes in Weh Island, Aceh, Indonesia

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9641 ◽  
Author(s):  
Nur Fadli ◽  
Siti Azizah Mohd Nor ◽  
Ahmad Sofiman Othman ◽  
Hizir Sofyan ◽  
Zainal A. Muchlisin
Keyword(s):  
Dna Barcoding ◽  
Dna Barcode ◽  
Reef Fishes ◽  
Morphological Identification ◽  
Species Discovery ◽  
Indonesian Archipelago ◽  
Aceh Province ◽  
Commercially Important ◽  
Catch Composition ◽  
Fisheries Statistics

Knowledge on the precise identification of fish resources is critical for sustainable fisheries management. This study employs the DNA barcoding approach to generate a molecular taxonomic catalogue of commercially important reef fishes in the waters of Weh Island (Aceh Province), the most northerly inhabited island in the biodiverse Indonesian Archipelago. The waters not only support artisanal fisheries but also a feeder for the industry in the greater island of Aceh. In total, 230 specimens from 72 species belonging to 32 genera and 17 families were DNA barcoded, representing a major segment of the captured reef fish taxa and a quarter of fish species diversity that had previously been recorded. The sequence read lengths were 639 bp revealing 359 conserved sites, 280 variable sites, 269 parsimony informative and 11 singletons. Our molecular findings paralleled the morphological identification with no evidence of cryptic species or new species discovery. This study is a significant contribution to the fisheries statistics of this area, which would facilitate assessment of species catch composition and hence for strategizing management plans. It is an important input to the DNA barcode library of Indonesian marine fishes and to the global DNA barcode entries in general.

First records raise questions: DNA barcoding of Odonata in the middle of the Mediterranean

Genome ◽  
2020 ◽  
pp. 1-11 ◽  
Author(s):  
Tomasz Rewicz ◽  
Arnold Móra ◽  
Grzegorz Tończyk ◽  
Ada Szymczak ◽  
Michal Grabowski ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Related Species ◽  
Dna Barcode ◽  
Taxonomic Status ◽  
Index Number ◽  
Reference Library ◽  
Nuclear Markers ◽  
Closely Related Species ◽  
Maltese Islands ◽  
Morphological Species

We present the results of the first-ever DNA barcoding study of odonates from the Maltese Islands. In total, 10 morphologically identified species were collected during a two-week long expedition in 2018. Eighty cytochrome c oxidase subunit I (COI) barcodes were obtained from the collected specimens. Intra- and interspecific distances ranged from 0.00% to 2.24% and 0.48% to 17.62%, respectively. Successful species identification based on ascribing a single morphological species to a single Barcode Index Number (BIN) was achieved for eight species (80%). In the case of two species, Ischnura genei and Anax parthenope, BINs were shared with other closely related species. The taxonomic status of I. genei is questionable and the phylogenetic relationship between A. imperator/parthenope is not clear. Further studies involving a series of adult specimens collected in a wide spatial range and nuclear markers are necessary to resolve these cases. Therefore, this dataset serves as an initial DNA barcode reference library for Maltese odonates, within a larger project: Aquatic Macroinvertebrates DNA Barcode Library of Malta.

scholarly journals Identification of Nepeta olgae Regel and phylogenetic status of some genera in subtribe Nepetinae (Lamiaceae) using DNA markers

2021 ◽  
Vol 38 ◽  
pp. 00087
Author(s):  
Elena Nikitina ◽  
Abdurashid Rakhmatov
Keyword(s):  
Dna Barcoding ◽  
Large Scale ◽  
Dna Barcode ◽  
Dna Barcodes ◽  
Biodiversity Monitoring ◽  
Reference Library ◽  
Accurate Identification ◽  
Unknown Species ◽  
Phylogenetic Status ◽  
Nuclear Its

The species level diversity is the reference unit for biodiversity accounting, should be systematized and include full information about the species. Reliable identification of any species is critical for a large-scale biodiversity monitoring and conservation. A DNA barcode is a DNA sequence that identifies a species by comparing the sequence of an unknown species with barcodes of a known species sequence database. Accurate identification of important plants is essential for their conservation, inventory. The species diversity assessing exampled on the subtribe Nepetinae (Lamiaceae) representatives, growing in Uzbekistan is given, using DNA barcoding method. The study was aimed to identify indigenous important plants with the nuclear (ITS) and plastid (matK, rbcL, trnL-F) genomes. This work demonstrates the phylogenetic relationships of some genera within the subtribe Nepetinae Coss. & Germ. (Lamiaceae), based on ITS locus gene. All results indicate that the DNA barcoding tool can be successfully used to reliably identify important plants, to inventory the botanical resources of Uzbekistan and to create a reference library of DNA barcodes. So, the combination of three-four locus gene is a good candidate for this approach.

scholarly journals BIN overlap confirms transcontinental distribution of pest aphids (Hemiptera: Aphididae)

2019 ◽  
Author(s):  
Muhammad Tayyib Naseem ◽  
Muhammad Ashfaq ◽  
Arif Muhammad Khan ◽  
Akhtar Rasool ◽  
Muhammad Asif ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Insect Pests ◽  
Rhopalosiphum Padi ◽  
Aphis Gossypii ◽  
Dna Barcode ◽  
Reference Sequence ◽  
Neighbor Joining ◽  
Reference Library ◽  
Index Numbers ◽  
Intraspecific Divergence

AbstractDNA barcoding is highly effective for identifying specimens once a reference sequence library is available for the species assemblage targeted for analysis. Despite the great need for an improved capacity to identify the insect pests of crops, the use of DNA barcoding is constrained by the lack of a well-parameterized reference library. The current study begins to address this limitation by developing a DNA barcode reference library for the pest aphids of Pakistan. It also examines the affinities of these taxa with conspecific taxa from other geographic regions based on both conventional taxonomy and Barcode Index Numbers (BINs). A total of 809 aphids were collected from 123 plant species at 87 sites across Pakistan. Morphological study and DNA barcoding allowed 774 specimens to be identified to one of 42 species while the others were placed to a genus or subfamily. The 801 sequences obtained from these specimens were assigned to 52 BINs whose monophyly were supported by neighbor-joining (NJ) clustering and Bayesian inference. The 42 species were assigned to 41 BINs with 38 showing BIN concordance; one species (Rhopalosiphum padi) was assigned to two BINs, while two others (Aphis affinis, Aphis gossypii) were assigned to the same BIN, while one species (Aphis astragalina) lacked a qualifying sequence. The 42 Linnaean species were represented on BOLD by 7,870 records from 69 countries. Combining these records with those from Pakistan produced to 60 BINs with 12 species showing a BIN split and three a BIN merger. Geo-distance correlations showed that intraspecific divergence values for 18 of 37 species were not affected by the distance between populations. Forty four of the 52 BINs from Pakistan had counterparts in 73 countries across six continents, documenting the broad distributions of pest aphids.

scholarly journals BIOSCAN - Revealing Eukaryote Diversity, Dynamics, and Interactions

2019 ◽  
Vol 3 ◽  
Author(s):  
Donald Hobern ◽  
Paul Hebert
Keyword(s):  
Dna Barcoding ◽  
Species Interactions ◽  
Dna Barcode ◽  
Cost Effective ◽  
Global Scale ◽  
Lessons Learned ◽  
Reference Library ◽  
Local Conservation ◽  
Insect Fauna ◽  
Sequencing Platforms

Between 2010 and 2015, the International Barcode of Life (iBOL) consortium successfully completed the BARCODE 500K project, a $125 million effort that delivered DNA barcode coverage for 500,000 species. BIOSCAN is a seven-year program (2019-2025) that builds on this foundation, expanding coverage of the barcode reference library to two million species and operationalising metabarcoding for eukaryote communities globally. BIOSCAN will scan species assemblages from at least 2,500 ecosystems and will codify species interactions for at least 2,500 sites. DNA barcoding is a well-established approach for rapid, cost-effective species diagnosis, with many applications in support of taxonomy, biosecurity, conservation, and monitoring. Uptake has been particularly significant in hyperdiverse invertebrate groups where morphological approaches to species identification are often limiting (because of the scale of diversity and the small number of expert taxonomists) or inapplicable (for example in associating individuals from different life stages). The barcode reference library maintained as BOLD Systems by the Centre for Biodiversity Genomics in Guelph, Ontario is a significant biodiversity informatics infrastructure for bridging genomics and classical taxonomy, collections research, and field surveys. Effort across multiple years in Canada has delivered a library of reference sequences for the COI mitochondrial barcode that covers most of the known insect fauna for the country, enabling a comprehensive assessment of Canadian arthropod diversity (Hebert et al. 2016, Langor and Sheffield 2019). The Global Malaise Trap Program is expanding lessons learned in Canada to support species inventories in new regions such as Kruger National Park in South Africa. As DNA barcode libraries approach completeness for any site, analysis can employ metabarcoding to lower costs significantly for monitoring programs that track changes in species composition. Data from this program, and from barcode-based exploration in other regions, will greatly expand the fraction of biodiversity that can be monitored and compared over time and space. GBIF has collected more than one billion species records, but around 60% of these are for birds, with another 25% for vascular plants. Metabarcoding offers the opportunity for a wider selection of taxa to be included in global data sets and in support of local conservation and planning. The BIOSCAN program, launched by iBOL in 2019, seeks to operationalise DNA barcoding at the global scale for development of species inventories and preliminary exploration of undescribed diversity, for surveying community composition across the world's ecosystems, and codifying species interactions (the symbiome). BIOSCAN will exploit the latest advances in sequencing platforms to lower costs, increase precision, and accelerate processing of samples, to speed the uptake of DNA barcoding for protecting life on Earth.

scholarly journals An overview of molecular identification of insect fauna with special emphasis on chalcid wasps (Hymenoptera: Chalcidoidea) of India

2018 ◽  
Vol 111 (1) ◽  
pp. 229
Author(s):  
Ajaz RASOOL ◽  
Tariq AHMAD ◽  
Bashir Ahmad GANAI ◽  
Shaziya GULL
Keyword(s):  
Dna Barcoding ◽  
Dna Sequences ◽  
Biological Diversity ◽  
Global Climate ◽  
Biological Effects ◽  
Dna Barcode ◽  
Habitat Destruction ◽  
Insect Fauna ◽  
Processed Material ◽  
The Face

Identifying organisms has grown in importance as we monitor the biological effects of global climate change and attempt to preserve species diversity in the face of accelerating habitat destruction. Classical taxonomy falls short in this race to catalogue biological diversity before it disappears. Differentiating subtle anatomical differences between closely related species requires the subjective judgment of highly trained specialists – and few are being trained in institutes today. DNA barcodes allow non-experts to objectively identify species – from small, damaged, or even industrially processed material. The aim of DNA barcoding is to establish a shared community resource of DNA sequences commonly used for identification, discrimination or taxonomic classification of organisms. It is a method that uses a short genetic marker in an organism's DNA to identify and distinguish its belonging from particular species, varieties or inter varieties. This simple technique has attracted attention from taxonomists, ecologists, conservation biologists, agriculturists, plant-quarantine officers and studies using the DNA barcode has rapidly increased. The extreme diversity of insects and their economical, epidemiological and agricultural importance have made them a major target of DNA barcoding. In this review, we present an overview of DNA barcoding of insects with emphasis on Chalcid wasps of India.

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