scholarly journals Digital Droplet PCR-Based Environmental DNA Tool for Monitoring Cryptocaryon irritans in a Marine Fish Farm from Hong Kong

Diversity ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 350
Author(s):  
Hin Hung Tsang ◽  
Jose A. Domingos ◽  
Jacob A. F. Westaway ◽  
Maximilian H. Y. Kam ◽  
Roger Huerlimann ◽  
...  
Keyword(s):  
Hong Kong ◽  
Marine Fish ◽  
Environmental Control ◽  
Environmental Dna ◽  
Fish Farm ◽  
Fish Farms ◽  
Cryptocaryon Irritans ◽  
Digital Droplet Pcr ◽  
Physical Chemical ◽  
Droplet Pcr

The adoption of new investigative strategies based on environmental DNA (eDNA) can be used to monitor parasites, associated bacterial microbiomes, and physical-chemical parameters in fish farms. In this study, we used the economically important and globally distributed fish ciliate parasite Cryptocaryon irritans as a model to understand the parasite abundance and potential drivers of its presence in marine fish farms. Environmental (rainfall) and physical-chemical (temperature, oxygen, salinity, pH) data collected from a marine fish farm in Hong Kong were analyzed together with the eDNA approach targeting C. irritans abundance based on digital droplet PCR and 16S metagenomics to determine associations and triggers between parasites and specific bacterial groups. Rainfall and temperature demonstrated positive associations with high abundance of C. irritans (eDNA) at the studied marine fish cage farm. However, rainfall was the only parameter tested that demonstrated a significant association with parasite eDNA, indicating that the raining season is a risky period for fish farmers in Hong Kong. Coraliomargarita was the bacterial genus with the most significant relationship with low abundance of C. irritans in water. Understanding the environmental triggers of ciliate parasites propagation and associated bacterial microbiome could elucidate new insights into environmental control, microbial management, and promote the reduction of chemical use in marine fish farms.

Environmental Description in the Design of Fish Farms at Exposed Locations

2017 ◽  
Author(s):  
David Kristiansen ◽  
Vegard Aksnes ◽  
Biao Su ◽  
Pål Lader ◽  
Hans V. Bjelland
Keyword(s):  
Marine Fish ◽  
Environmental Conditions ◽  
Measurement Data ◽  
Field Measurements ◽  
Fish Farm ◽  
Fish Farms ◽  
Coastal Regions ◽  
Waves And Currents ◽  
Farm Structures

This paper addresses the description of exposure from waves and currents in coastal regions for design of marine fish farms. Representative descriptions of environmental conditions are important inputs to the design and dimensioning of reliable fish farm structures. A trend with moving production to more exposed sites and introduction of new and novel fish farm structures increase the need for more precise descriptions of the marine environment to keep control of uncertainties in design. Dedicated field measurements at two exposed aquaculture sites from February to December 2016 are presented. Results from statistical analyses of the measurement data demonstrate that common practice for characterization of exposure in design of fish farms has several deficiencies that should be improved to reduce uncertainties in design.

Design Rules for Marine Fish Farms in Norway: Calculation of the Structural Response of Such Flexible Structures to Verify Structural Integrity

2004 ◽  
Author(s):  
Are Johan Berstad ◽  
Harald Tronstad ◽  
Anders Ytterland
Keyword(s):  
Marine Fish ◽  
Structural Integrity ◽  
Flexible Structures ◽  
Structural Response ◽  
Fish Farm ◽  
Practical Calculation ◽  
Fish Farms ◽  
Good Correspondence ◽  
Design Rules ◽  
Farm Structures

As of April 1st 2004 all new marine fish farms in Norway need to be certified to comply with technical criteria in a new Norwegian Standard, NS 9415 (NAS, 2003). This paper gives an overview of the design rules. Marine fish farms have a strongly flexible hydroelastic behavior. The design rules have revealed the need for calculations to verify the structural capacity of such facilities. This paper describes how loads and response are derived on the fish farm structures. In order to account for the large geometrical deflections occurring in fish farm components such as the net and anchor cables which interacts with stiffer structural components, time domain simulations analysis of such facilities is required to assess the structural integrity. This paper report a proposed practical calculation method and results from model tests have been carried out to validate calculations. Good correspondence was shown. Additionally some applications are described.

scholarly journals Application of Environmental DNA for Monitoring Red Sea Bream Iridovirus at a Fish Farm

2021 ◽  
Author(s):  
Yasuhiko Kawato ◽  
Tohru Mekata ◽  
Mari Inada ◽  
Takafumi Ito
Keyword(s):  
Red Sea ◽  
Disease Outbreaks ◽  
Environmental Dna ◽  
Fish Farm ◽  
Sea Bream ◽  
Practical Application ◽  
Red Sea Bream ◽  
Fish Farms ◽  
Few Data ◽  
Waterborne Viruses

Environmental DNA (eDNA) could be applied in monitoring waterborne viruses of aquatic animals. However, there are few data for practical application of eDNA in fish farms for the control of disease outbreaks.

Water Quality in the Priority Watercourses of Hong Kong in Respect of Various Environmental Control Measures

1991 ◽  
Vol 23 (1-3) ◽  
pp. 85-91 ◽  
Author(s):  
Ho Kin-chung
Keyword(s):  
Water Quality ◽  
Hong Kong ◽  
Environmental Control ◽  
Control Measures ◽  
Livestock Waste ◽  
Water Control ◽  
Control Zone ◽  
Town Development ◽  
Control Scheme ◽  
Term Monitoring

The water quality in the 12 priority watercourses of Hong Kong was appraised in respect of the various environmental control measures being undertaken. It was noted that water quality in Lam Tsuen River had been significantly improved since 1983. This is attributed to recent resumption of unsewered lands for town development, training of river basin to increase flow, and declaration of the catchment as a “Water Control Zone” under the Water Pollution Control Ordinance. In contrast with the other heavily polluted watercourses to which little abatement measures were implemented, the water qualities of Shing Mun River and Tuen Mun River were slightly upgraded because of the efforts to rectify unauthorized industrial discharges back to foul sewer and provision of interceptors and sewers to villages. The 10 year Livestock Waste Control Scheme enforced on 24 June 1989 was found in parallel with BOD and suspended solids decrease in watercourses. To assess its effectiveness, however, a longer term monitoring is required to get a conclusive result.

scholarly journals Development of a Hydropower Turbine Using Seawater from a Fish Farm

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 266
Author(s):  
Md Rakibuzzaman ◽  
Sang-Ho Suh ◽  
Hyoung-Ho Kim ◽  
Youngtae Ryu ◽  
Kyung Yup Kim
Keyword(s):  
Power Plants ◽  
Guide Vane ◽  
Cross Flow ◽  
Synchronous Generator ◽  
Fish Farm ◽  
Hydropower Plant ◽  
Fish Farms ◽  
Data Simulation ◽  
Small Hydropower ◽  
Hydropower Plants

Discharge water from fish farms is a clean, renewable, and abundant energy source that has been used to obtain renewable energy via small hydropower plants. Small hydropower plants may be installed at offshore fish farms where suitable water is obtained throughout the year. It is necessary to meet the challenges of developing small hydropower systems, including sustainability and turbine efficiency. The main objective of this study was to investigate the possibility of constructing a small hydropower plant and develop 100 kW class propeller-type turbines in a fish farm with a permanent magnet synchronous generator (PMSG). The turbine was optimized using a computer simulation, and an experiment was conducted to obtain performance data. Simulation results were then validated with experimental results. Results revealed that streamlining the designed shape of the guide vane reduced the flow separation and improved the efficiency of the turbine. Optimizing the shape of the runner vane decreased the flow rate, reducing the water power and increasing the efficiency by about 5.57%. Also, results revealed that tubular or cross-flow turbines could be suitable for use in fish farm power plants, and the generator used should be waterproofed to avoid exposure to seawater.

scholarly journals Monitoring EGFR C797S mutation in Japanese NSCLC patients with serial cell free DNA evaluation using digital droplet PCR

2021 ◽  
Author(s):  
Ryo Ariyasu ◽  
Ken Uchibori ◽  
Takaaki Sasaki ◽  
Mika Tsukahara ◽  
Kazuma Kiyotani ◽  
...  
Keyword(s):  
Cell Free Dna ◽  
Digital Droplet Pcr ◽  
Free Dna ◽  
Droplet Pcr ◽  
Nsclc Patients

scholarly journals P34.06 The Clinical Utility of Liquid Biopsy by Digital Droplet PCR in Patients with Advanced NSCLC

2021 ◽  
Vol 16 (3) ◽  
pp. S417
Author(s):  
V. Lamberts ◽  
M. Aldea ◽  
L. Mezquita ◽  
C. Jovelet ◽  
D. Vasseur ◽  
...  
Keyword(s):  
Liquid Biopsy ◽  
Clinical Utility ◽  
Advanced Nsclc ◽  
Digital Droplet Pcr ◽  
Droplet Pcr

Feasibility of Repurposing Offshore Decommissioned Gas Rigs into Fish Farms

2021 ◽  
Author(s):  
Saptarshi Pal ◽  
Chengi Kuo
Keyword(s):  
North Sea ◽  
Net Present Value ◽  
Cost Benefit Analysis ◽  
Storage System ◽  
Artificial Reef ◽  
Cost Benefit ◽  
Fish Farm ◽  
Fish Feed ◽  
Offshore Platforms ◽  
Fish Farms

Abstract In the past 70 years the world has relied extensively for its energy needs based on hydrocarbons produced significantly offshore. In recent years many installations with fixed platforms and pipelines are reaching the end of their useful life and are required by law to be decommissioned and removed if an approved alternative use cannot be found. This process coincides with focus on decarbonization arising from global warming and climate change. The conventional way of decommissioning is to remove the structure and take it onshore for disposal. Such an activity costs around £28 million for smaller UKCS installations in the Southern North Sea. Possible alternative solutions include their use as a research-leisure complex and artificial reef. Such an approach would have less impact on the environment and it is therefore worthwhile to explore the feasibility of repurposing these decommissioned UKCS platforms. The paper begins by highlighting the background to UKCS offshore decommissioning and farming fish life-cycle. This is followed by a critical review of the three options of total and partial removals and leave-on-site. It is found that repurposing decommissioned platforms for aquaculture farm has not been given sufficient attention and thus offers scope for a project to explore the feasibility of such a solution. Existing offshore fish farming in various countries are examined before using a decision-making matrix to select the most suitable UKCS installation for conversion and this led to using a normally unattended gas platform for the case study. The focus for this paper is on design and operation of an unattended fish farm and its cost benefit analysis. The former covers fish cage selection, capacity calculation, fish handling procedures, fish feed characteristics, feed demand, designing feed logistics and storage system. The processing facilities are layout on two decks and power needs are generated using a hybrid system of diesel and Li-ion battery. The possibility of using renewable sources by connecting to wind energy grids was also considered. For the latter capital and operating expenditure, revenue generated and maintenance costs are estimated before performing net present value prediction of the profitability of the fish farm over 10 years with for example up to 8 cages and three discount rates. The main conclusions derived are: It is technically feasible to convert a decommissioned gas platform to a fish farm and the operation can be economic. However, liability transfer implications in a repurposed offshore decommissioned gas platforms to fish farms were not established to verify the project viability. The conversion of unattended offshore gas platforms in the UKCS to an automated offshore fish farm is a novel solution which has not been implemented in the North Sea before. The work will provide an economic and environmental friendly solution to decommissioning offshore platforms and provide with a possible profitable investment.

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