scholarly journals Highlighting the Crude Oil Bioremediation Potential of Marine Fungi Isolated from the Port of Oran (Algeria)

Diversity ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 196 ◽  
Author(s):  
Ahlem Maamar ◽  
Marie-Elisabeth Lucchesi ◽  
Stella Debaets ◽  
Nicolas Nguyen van Long ◽  
Maxence Quemener ◽  
...  
Keyword(s):  
Species Richness ◽  
Carbon Source ◽  
Crude Oil ◽  
Marine Environment ◽  
Marine Fungi ◽  
Oil Pollution ◽  
Fungal Communities ◽  
Contaminated Site ◽  
Fungal Isolates ◽  
Seawater Samples

While over hundreds of terrestrial fungal genera have been shown to play important roles in the biodegradation of hydrocarbons, few studies have so far focused on the fungal bioremediation potential of petroleum in the marine environment. In this study, the culturable fungal communities occurring in the port of Oran in Algeria, considered here as a chronically-contaminated site, have been mainly analyzed in terms of species richness. A collection of 84 filamentous fungi has been established from seawater samples and then the fungi were screened for their ability to utilize and degrade crude oil. A total of 12 isolates were able to utilize crude oil as a unique carbon source, from which 4 were defined as the most promising biodegrading isolates based on a screening test using 2,6-dichlorophenol indophenol as a proxy to highlight their ability to metabolize crude oil. The biosurfactant production capability was also tested and, interestingly, the oil spreading and drop-collapse tests highlighted that the 4 most promising isolates were also those able to produce the highest quantity of biosurfactants. The results generated in this study demonstrate that the most promising fungal isolates, namely Penicillium polonicum AMF16, P. chrysogenum AMF47 and 2 isolates (AMF40 and AMF74) affiliated to P. cyclopium, appear to be interesting candidates for bioremediation of crude oil pollution in the marine environment within the frame of bioaugmentation or biostimulation processes.

scholarly journals The culturable mycobiota associated with the Mediterranean sponges Aplysina cavernicola, Crambe crambe and Phorbas tenacior

2019 ◽  
Vol 366 (24) ◽  
Author(s):  
Elena Bovio ◽  
Estelle Sfecci ◽  
Anna Poli ◽  
Giorgio Gnavi ◽  
Valeria Prigione ◽  
...  
Keyword(s):  
Microbial Community ◽  
Mediterranean Sea ◽  
Marine Environment ◽  
Marine Fungi ◽  
Species Level ◽  
Fungal Communities ◽  
The Mediterranean ◽  
Satellite Analysis ◽  
First Time

ABSTRACT Marine fungi are part of the huge and understudied biodiversity hosted in the sea. To broaden the knowledge on fungi inhabiting the Mediterranean Sea and their role in sponge holobiont, three sponges namely Aplysina cavernicola, Crambe crambe and Phorbas tenacior were collected in Villefranche sur Mer, (France) at about 25 m depth. The fungal communities associated with the sponges were isolated using different techniques to increase the numbers of fungi isolated. All fungi were identified to species level giving rise to 19, 13 and 3 species for P. tenacior, A. cavernicola and C. crambe, respectively. Of note, 35.7% and 50.0% of the species detected were either reported for the first time in the marine environment or in association with sponges. The mini-satellite analysis confirmed the uniqueness of the mycobiota of each sponge, leading to think that the sponge, with its metabolome, may shape the microbial community.

scholarly journals Changes of Heart Rate and Lipid Composition in Mytilus Edulis and Modiolus Modiolus Caused by Crude Oil Pollution and Low Salinity Effects

2021 ◽  
Vol 11 (2) ◽  
pp. 46-60
Author(s):  
Igor Bakhmet ◽  
Natalia Fokina ◽  
Tatiana Ruokolainen
Keyword(s):  
Heart Rate ◽  
Crude Oil ◽  
Mytilus Edulis ◽  
Lipid Composition ◽  
Oil Pollution ◽  
Cardiac Activity ◽  
Blue Mussels ◽  
Low Salinity ◽  
Crude Oil Pollution ◽  
Modiolus Modiolus

Blue mussels, Mytilus edulis, inhabiting tidal zones, are naturally exposed to fluctuating environmental conditions (e.g., fluctuations in temperature and salinities), while horse mussels, Modiolus modiolus, live under relatively invariable shelf water conditions. The present investigation tested the hypothesis: blue mussels, in comparison to horse mussels, have an increased ability to tolerate the stress of pollution combined with low salinity. To assess the response of blue mussels and horse mussels to oil pollution at seawater salinities of 25 psu (normal) and 15 psu (low), we used a combination of heart rate and lipid composition as physiological and biochemical indicators, respectively. A sharp decrease in heart rate as well as important fluctuations in cardiac activity was observed under all oil concentrations. Modifications in the concentrations of the main membrane lipid classes (phosphatidylcholine, phosphatidylethanolamine, and cholesterol) and storage lipids (primarily triacylglycerols) in response to different crude oil concentrations were time- and dose-dependent. Both chosen indicators showed a high sensitivity to crude oil contamination. Furthermore, both bivalve species showed similar responses to oil pollution, suggesting a universal mechanism for biochemical adaptation to crude oil pollution.

scholarly journals Production of High Purity Biosurfactants Using Heavy Oil Residues as Carbon Source

Energies ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3557
Author(s):  
Athina Mandalenaki ◽  
Nicolas Kalogerakis ◽  
Eleftheria Antoniou
Keyword(s):  
Carbon Source ◽  
Heavy Oil ◽  
Oil Pollution ◽  
Batch Reactor ◽  
Production Method ◽  
Promising Alternative ◽  
Heavy Oil Residues ◽  
Oil Residue ◽  
Increasing Demand ◽  
Heavy Oil Residue

Typically, oil pollution cleanup procedures following first response actions include dispersion. Crude oil is biodegradable, and its bioavailability can be increased when dispersed into very fine droplets by means of chemical surfactants. Although their use is widely spread in many applications, the latter may prove toxic, depending on the extent of use. The use of biological means, such as bioremediation and biosurfactants, has emerged over the past years as a very promising ‘green’ alternative technology. Biosurfactants (BSs) are amphiphilic molecules produced by microorganisms during biodegradation, thus increasing the bioavailability of the organic pollutants. It is their biodegradability and low toxicity that render BSs as a very promising alternative to the synthetic ones. Alcanivorax borkumensis SK2 strain ability to produce BSs, without any impurities from the substrate, was investigated. The biosurfactant production was scaled up by means of a sequencing batch reactor (SBR) and a heavy oil residue substrate as the carbon source. The product is free from substrate impurities, and its efficiency is tested on oil bioremediation in the marine environment. The product’s dispersion efficiency was determined by the baffled flask test. The production method proposed can have a significant impact to the market, given the ever-increasing demand for ecologically friendly, reliable, commercially viable and economically competitive environmental cleanup techniques.

Marine Environmental Damage: The Compensability of Ecosystem Service Loss in International Law

2019 ◽  
Vol 34 (4) ◽  
pp. 602-641
Author(s):  
Günther Handl
Keyword(s):  
International Law ◽  
Marine Environment ◽  
Oil Pollution ◽  
Environmental Damage ◽  
Emerging Trends ◽  
Full Compensation ◽  
Environmental Loss ◽  
National Practice ◽  
Marine Environmental

AbstractKey maritime conventions governing liability and compensation for pollution of the marine environment, foremost among them the 1992 Civil Liability for Oil Pollution Convention and the 2003 Supplementary Fund Protocol (the CLC/Fund regime), exclude compensation for pure environmental loss. This article discusses whether anything less than full compensation of damage to the marine environment, including the loss of ecosystem services, comports with contemporary international public policy or law. After reviewing and rejecting traditional arguments opposing such compensability, the article contrasts the CLC/Fund regime’s environmental claims practice with emerging trends in decision on the international legal plane and in select domestic legal systems, all of which support full compensation. The article thus concludes that an adjustment of the CLC/Fund regime’s environmental claims approach is desirable to align it with this international (and national) practice and thereby to protect the long-term integrity of the regime itself.

Response of a Newly Identified Trichoderma Harzianum KY488466 to Crude Oil Pollution and Its Expression of Peroxidase Genes

2017 ◽  
Author(s):  
Segun Gbolagade Jonathan ◽  
Michael Asemoloye ◽  
Rafiq Ahmad ◽  
O. Joseph Olawuyi ◽  
Damilola Adejoye
Keyword(s):  
Crude Oil ◽  
Trichoderma Harzianum ◽  
Oil Pollution ◽  
Crude Oil Pollution

scholarly journals Study of a plugging microbial consortium using crude oil as sole carbon source

2008 ◽  
Vol 5 (4) ◽  
pp. 367-374 ◽  
Author(s):  
Jing Wang ◽  
Guiwen Yan ◽  
Mingquan An ◽  
Jieli Liu ◽  
Houming Zhang ◽  
...  
Keyword(s):  
Carbon Source ◽  
Crude Oil ◽  
Sole Carbon Source ◽  
Microbial Consortium

scholarly journals Sublethal Effects of Crude Oil and Chemical Dispersants on Multiple Life History Stages of the Eastern Oyster, Crassostrea virginica

2020 ◽  
Vol 8 (10) ◽  
pp. 808
Author(s):  
Sara M. Garcia ◽  
Kevin T. Du Clos ◽  
Olivia H. Hawkins ◽  
Brad J. Gemmell
Keyword(s):  
Life History ◽  
Crude Oil ◽  
Crassostrea Virginica ◽  
Sublethal Effects ◽  
Oil Pollution ◽  
Eastern Oyster ◽  
Clearance Rates ◽  
Important Species ◽  
Life History Stages ◽  
Oil Exposure

The eastern oyster Crassostrea virginica is an ecologically and economically important species that is vulnerable to oil pollution. We assessed sublethal effects of soluble fractions of crude oil alone (WAF) and crude oil in combination with Corexit 9500 dispersant (CEWAF) on oysters at three life history stages. Veliger swimming, pediveliger settlement, and adult clearance rates were quantified after 24 h exposures to the contaminants. Veliger swimming speeds were not significantly impacted by 24 h exposures to WAF or CEWAF. A larger proportion of veligers were inactive following WAF and CEWAF exposure as compared to the control, but the effect was greater for pediveligers, and pediveliger settlement in the highest concentration CEWAF treatment decreased by 50% compared to controls. Thus, pediveligers may be particularly vulnerable to oil exposure. In the adults, we found significant clearance rates reductions that persisted 33 days after acute exposure to CEWAF. Knowledge of sublethal effects of oil and dispersant at multiple life history stages aids understanding of how this important species will respond to an oil spill.

scholarly journals Isolation and Characterization of a Biosurfactant Producing Strain Planococcus sp. XW-1 from the Cold Marine Environment

2022 ◽  
Vol 19 (2) ◽  
pp. 782
Author(s):  
Ping Guo ◽  
Weiwei Xu ◽  
Shi Tang ◽  
Binxia Cao ◽  
Danna Wei ◽  
...  
Keyword(s):  
Low Temperature ◽  
Carbon Source ◽  
Crude Oil ◽  
Surface Activity ◽  
Yellow Sea ◽  
High Surface ◽  
Sole Source ◽  
The Yellow Sea ◽  
Isolation And Characterization ◽  
First Time

One cold-adapted strain, named Planococcus sp. XW-1, was isolated from the Yellow Sea. The strain can produce biosurfactant with petroleum as sole source of carbon at low temperature (4 °C). The biosurfactant was identified as glycolipid-type biosurfactant species by thin-layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR). It reduced the surface tension of water to 26.8 mN/m with a critical micelle concentration measurement of 60 mg/L. The produced biosurfactant possesses high surface activity at wide ranges of temperature (−18–105 °C), pH values (2–12), and salt concentrations (1–18%). The biosurfactant exhibited higher surface activity and higher growth rate of cells with hexadecane and diesel as carbon source. The strain Planococcus sp. XW-1 was also effective in degrading crude oil, after 21 days of growth at 4 °C in medium with 1% crude oil and 1% (v/v) bacteria broth, 54% of crude oil was degraded. The results suggest that Planococcus sp. XW-1 is a promising candidate for use in the bioremediation of petroleum-contaminated seawater in the Yellow Sea during winter. This study reported for the first time that Planococcus isolated from the Yellow Sea can produce biosurfactant using petroleum as the sole carbon source at low temperature (4 °C), showing its ecological role in the remediation of marine petroleum pollution.

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