scholarly journals Using ISSR Genomic Fingerprinting to Study the Genetic Differentiation of Artemia Leach, 1819 (Crustacea: Anostraca) from Iran and Neighbor Regions with the Focus on the Invasive American Artemia franciscana

Diversity ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 132 ◽  
Author(s):  
Amin Eimanifar ◽  
Alireza Asem ◽  
Pei-Zheng Wang ◽  
Weidong Li ◽  
Michael Wink
Keyword(s):  
Genetic Variability ◽  
Salt Lake ◽  
Artemia Franciscana ◽  
Coi Gene ◽  
Source Population ◽  
Genomic Fingerprinting ◽  
Mt Dna ◽  
Private Haplotype ◽  
Western Asia ◽  
Native Populations

Due to the rapid developments in the aquaculture industry, Artemia franciscana, originally an American species, has been introduced to Eurasia, Africa and Australia. In the present study, we used a partial sequence of the mitochondrial DNA Cytochrome Oxidase subunit I (mt-DNA COI) gene and genomic fingerprinting by Inter-Simple Sequence Repeats (ISSRs) to determine the genetic variability and population structure of Artemia populations (indigenous and introduced) from 14 different geographical locations in Western Asia. Based on the haplotype spanning network, Artemia urmiana has exhibited higher genetic variation than native parthenogenetic populations. Although A. urmiana represented a completely private haplotype distribution, no apparent genetic structure was recognized among the native parthenogenetic and invasive A. franciscana populations. Our ISSR findings have documented that despite that invasive populations have lower variation than the source population in Great Salt Lake (Utah, USA), they have significantly revealed higher genetic variability compared to the native populations in Western Asia. According to the ISSR results, the native populations were not fully differentiated by the PCoA analysis, but the exotic A. franciscana populations were geographically divided into four genetic groups. We believe that during the colonization, invasive populations have experienced substantial genetic divergences, under new ecological conditions in the non-indigenous regions.

scholarly journals Using ISSR Genomic Fingerprinting to Study the Genetic Differentiation of Artemia Leach, 1819 (Crustacea: Anostraca) from Iran and Neighbor Regions with the Focus on the Invasive American Artemia franciscana

2020 ◽  
Author(s):  
Amin Eimanifar ◽  
Alireza Asem ◽  
Pei-Zheng Wang ◽  
Weidong Li ◽  
Michael Wink
Keyword(s):  
Genetic Variability ◽  
Salt Lake ◽  
Artemia Franciscana ◽  
Coi Gene ◽  
Source Population ◽  
Genomic Fingerprinting ◽  
Mt Dna ◽  
Private Haplotype ◽  
Western Asia ◽  
Native Populations

Due to the rapid developments in aquaculture industry, Artemia franciscana, originally an American species, has been intentionally introduced to the Eurasia, Africa and Australia. In the present study, we used a partial sequence of the mitochondrial DNA Cytochrome Oxidase subunit I (mt-DNA COI) gene and genomic fingerprinting by Inter-Simple Sequence Repeats (ISSRs) to determine the genetic variability and population structure of Artemia populations (indigenous and introduced) from 14 different geographical locations in Western Asia. Based on the haplotype spanning network, Artemia urmiana has exhibited higher genetic variation than native parthenogenetic populations. Although A. urmiana represented a completely private haplotype distribution, no apparent genetic structure was recognized among the native parthenogenetic and invasive A. franciscana populations. Our ISSR findings have documented that despite invasive populations have lower variation than source population in Great Salt Lake (Utah, USA), they have significantly revealed higher genetic variability compare to the native populations in Western Asia. According to the ISSR results, the native populations were not fully differentiated by the PCoA analysis, but the exotic A. franciscana populations were geographically divided in four genetic groups. We believe that during the colonization, invasive populations have experienced substantial genetic divergences, under new ecological conditions in the non-indigenous regions.

scholarly journals The Impact of the Colonization of the Invasive American Artemia franciscana (Crustacea: Anostraca) on Genetic Differentiation in the United Arab Emirates (Asia)

2020 ◽  
Author(s):  
Alireza Asem ◽  
Rolf Schuster ◽  
Amin Eimanifar ◽  
Hao Lu ◽  
Muhan Li ◽  
...  
Keyword(s):  
Genetic Differentiation ◽  
San Francisco ◽  
United Arab Emirates ◽  
Salt Lake ◽  
Artemia Franciscana ◽  
Indigenous Populations ◽  
Coi Gene ◽  
Mediterranean Regions ◽  
Demographic Patterns ◽  
The Impact

Artemia franciscana, native to America, has recently colonized non-indigenous populations in Eurasia, Mediterranean regions and Australia. In present we sought to evaluate the potential effects of colonization of A. franciscana on genetic differentiation in the new environments in UAE. We used the COI marker to determine population genetic structure and identify the origins of exotic populations in UAE. Our findings have confirmed the colonization of both localities by A. franciscana. Genetic variation of invasive A. franciscana were exclusively lower than native population in Great Salt Lake and San Francisco Bay. Results have showed the studied population could not possibly have colonized directly from natural American localities, perhaps resulting from secondary introduction events from other non-indigenous populations. Genetic analysis have yielded different demographic patterns for invasive studied populations. Al Wathba Wetland Reserve (AWWR) population have represented demographic expansion. In contrast, Godolphin Lakes (GL) population was at demographic equilibrium. Neutrality tests have documented the excess of both recent and historical mutations in the COI gene pool of invasive AWWR Artemia throughout establishment in the new environment.

Genetic variability at allozyme markers in sycamore (Acer pseudoplatanus) populations from northwestern Italy

2007 ◽  
Vol 37 (2) ◽  
pp. 395-403 ◽  
Author(s):  
P. Belletti ◽  
I. Monteleone ◽  
D. Ferrazzini
Keyword(s):  
Genetic Variability ◽  
Single Gene ◽  
Acer Pseudoplatanus ◽  
Effective Number ◽  
Expected Heterozygosity ◽  
Slight Excess ◽  
Forest Reproductive Material ◽  
Native Populations ◽  
Reproductive Material ◽  
The Mean

Genetic diversity and differentiation of 12 native populations of sycamore ( Acer pseudoplatanus L.) from northwestern Italy were investigated by means of variation detected in 10 polymorphic isozyme systems encoded by 16 loci. The trees showed relatively large genetic variability (mean number of alleles per locus = 2.78, effective number of alleles per locus = 1.47, percentage of polymorphic loci = 96.9, expected heterozygosity = 0.280) but small interpopulational variation (FST = 0.019), suggesting the presence of a single gene pool. A slight excess of heterozygotes was found, and the overall inbreeding coefficient (FIS) was –0.038. There was a positive correlation between the age of the individuals sampled and heterozygosity, suggesting the occurrence of natural selection against homozygotes. No significant correlation was detected between genetic and geographic distances of populations, confirming the lack of any barriers to gene flow. Estimate of the mean effective number of migrants exchanged between populations was 4.399, based on the private alleles method. The contribution of the results in relation to terms of the most appropriate strategies to preserve genetic variability of sycamore and to collect forest reproductive material is discussed.

scholarly journals DIVERSITAS GENETIK DAN HAPLOGROUP KAMBING GEMBRONG BERSTATUS KRITIS DI KABUPATEN KARANGASEM, BALI (Genetic Diversity and Haplogroup of Endangered Gembrong Goat In Karangasem, Bali)

2016 ◽  
Vol 10 (2) ◽  
pp. 181-185
Author(s):  
M. Syamsul Arifin Zein ◽  
Sri Sulandari ◽  
Jakaria Jakaria ◽  
I Made Londra ◽  
Suprio Guntoro ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Genetic Variability ◽  
South Asia ◽  
Maternal Origin ◽  
Mitochondrial Dna Control Region ◽  
Hypervariable Segment ◽  
Phylogeny Analysis ◽  
Western Asia ◽  
Endangered Breed

This study was conducted to evaluate the genetic diversity and phylogeny of Gembrong goat. For this purpose, 21 goats from endangered breed in Karangasem Bali were used. Molecular analysis of genetic diversity and phylogeography used hypervariable segment 1 of mitochondrial DNA control region. The result showed that genetic variability of Gembrong goat was homogeneous with only one different sites, namely the substitution pyrimidines of C ↔ T (transitional). Phylogeny analysis results showed maternal origin of Gembrong goat is lineage (subhaplogroup) B1 with frequency of 100%. Haplogroup B were known has been domesticated from wild goat in western Asia, then headed to south Asia and infiltrated to southeast Asia, including Gembrong goat in Bali, Indonesia. As a conclusion, genetic diversity of Gembrong goat from remaining population in Karangasem very low and originate from lineages/haplogroup B1 with a frequency of 100%.

scholarly journals Genetic diversity and conservation of native populations of Maytenus Ilicifolia Mart. ex Reiss

2009 ◽  
Vol 69 (2) ◽  
pp. 447-453 ◽  
Author(s):  
AJ. Mossi ◽  
RL. Cansian ◽  
O. Leontiev-Orlov ◽  
JL. Cechet ◽  
AZ. Carvalho ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Genetic Variability ◽  
Rio Grande ◽  
Jaccard Coefficient ◽  
Rio Grande Do Sul ◽  
Mato Grosso ◽  
Native Populations ◽  
Maytenus Ilicifolia ◽  
Mato Grosso Do Sul

The aim of this work was to analyze genetic variability in 18 populations of Maytenus ilicifolia, and representatives of Maytenus aquifolia and Maytenus evonymoidis, collected in the states of Mato Grosso do Sul, Paraná, Santa Catarina and Rio Grande do Sul, using RAPD molecular markers. Considering total samples of the three species, 263 amplified fragments were identified, of which 72.2% showed to be polymorphous. The index of similarity (Jaccard coefficient) was on average 0.64 between M. ilicifolia and M. aquifolia; 0.47 between M. ilicifolia and M. evonymoidis; and 0.44 between M. aquifolia and M. evonymoidis. The analysis of groupings by the UPGMA algorithm allowed to clearly separate the three analyzed species. In determining the variability in M. ilicifolia, 222 bands were identified, on average 11.1 bands per primer, being 43.2% polymorphous. The index of similarity (Jaccard coefficient) in the bulks of each population in M. ilicifolia was, on average, 0.92 and the index of similarities among the populations was 0.83. The analysis of groupings with the UPGMA algorithm and the analysis of the main coordination (PCO), allowed the separation of the analyzed populations into three groups, the populations from the south of Rio Grande do Sul and the population from Mato Grosso do Sul standing out. A relation between the groupings found and the edaphoclimatic conditions of the collecting places was observed.

scholarly journals Molecular characterization of COI gene of Ixodes ricinus (Linnaeus, 1758) from Serbia

2014 ◽  
Vol 66 (3) ◽  
pp. 1243-1251
Author(s):  
Sanja Cakic ◽  
Miljana Mojsilovic ◽  
Darko Mihaljica ◽  
Marija Milutinovic ◽  
Andjeljko Petrovic ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Ixodes Ricinus ◽  
Intraspecific Variability ◽  
Balkan Peninsula ◽  
Coi Gene ◽  
Gene Sequences ◽  
Ricinus Tick ◽  
Pathogenic Agents ◽  
Font Color

The Ixodes ricinus tick is common in the central part of the Balkan Peninsula. It is a vector of pathogenic agents causing diseases in humans and animals. Little is known about the genetic structure of I. ricinus in this region. We have investigated intraspecific variability of the COI gene among I. ricinus ticks collected from different regions of Serbia, and the correlation between the various types of habitat and genetic variability of ticks. The obtained COI gene sequences are the first barcoding sequences of I. ricinus ticks collected at localities in Serbia. Intraspecific variability of these COI gene sequences was very low, and there was no correlation between the various types of habitat and genetic variability of ticks. Samples from isolated localities (canyon/gorge) showed no genetic differentiations from the majority of samples from open areas. [Projekat Ministarstva nauke Republike Srbije, br. ON 173006] <br><br><font color="red"><b> This article has been retracted. Link to the retraction <u><a href='http://dx.doi.org/10.2298/ABS1404689U'>10.2298/ABS1404689U</a><u></b></font>

scholarly journals Phylogenetic Relationships among Populations of the Vineyard Snail Cernuella virgata (Da Costa, 1778)

ISRN Zoology ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Jasna Puizina ◽  
Sanja Puljas ◽  
Željana Fredotović ◽  
Ivica Šamanić ◽  
Grgur Pleslić
Keyword(s):  
Genetic Variability ◽  
16S Rdna ◽  
Phylogenetic Relationships ◽  
Western Europe ◽  
Population Analysis ◽  
Shell Growth ◽  
Shell Height ◽  
Haplotype Network ◽  
Coi Gene ◽  
Island Population

Cernuella virgata (Da Costa, 1778) (Mollusca: Hygromiidae), commonly known as the “vineyard snail,” is endemic species in Mediterranean and Western Europe including the British Isles, but in the Eastern USA and Australia it represents an introduced invasive species. The present work examines the genetic variability and phylogenetic relationships among the four populations of this land snail sampled along the east Adriatic region of Croatia using mitochondrial markers (partial 16S rDNA and COI gene) in addition to traditional methods of shell’s shape analysis. All the three molecular-phylogenetic approaches (median joining haplotype network analysis and Bayesian analysis, as well as maximum likelihood analysis) revealed two-three major subnetworks for both 16S rDNA and COI, with a clear distinction between south Adriatic haplotypes (Pisak) and north Adriatic haplotypes (Krk and Cres). The population from Karlobag was comprised of both north and south haplotypes, thus representing a putative contact zone between these two groups. The morphometric analysis showed that individuals from Cres island population were statistically significantly wider and higher than individuals from Pisak population. Analysis of the SW/SH ratio and the relationship between shell width and shell height showed no differences in shell growth between the two examined populations, indicating equal shell growth and shape, which gives the possibility that differences in size of individuals between those two populations could be influenced by biotic (physiological) or abiotic (environmental) factors. This study represents the first analysis of genetic variability and relatedness among native populations of C. virgata.

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