scholarly journals Antioxidant and Anti-Aging Potential of Indian Sandalwood Oil against Environmental Stressors In Vitro and Ex Vivo

Cosmetics ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 53
Author(s):  
Véronique Francois-Newton ◽  
Andrew Brown ◽  
Philippe Andres ◽  
Madiiha Bibi Mandary ◽  
Carli Weyers ◽  
...  
Keyword(s):  
Human Skin ◽  
Active Ingredient ◽  
Ex Vivo ◽  
Environmental Stressors ◽  
Alpha Tocopherol ◽  
Santalum Album ◽  
Aging Effects ◽  
Sandalwood Oil ◽  
Skin Explants

Distilled from the heartwood of Santalum album, Indian sandalwood oil is an essential oil that historically has been used as a natural active ingredient in cosmetics to condition and brighten the skin. It has been documented to exhibit antioxidant, anti-inflammatory, and anti-proliferative activities. Here, we investigated the protective and anti-aging effects of Indian sandalwood oil in scavenging reactive oxygen species (ROS) in HaCaT cells and in human skin explants after exposure to oxidative stress. Using a probe DCFH-DA, the antioxidant capacity of Indian sandalwood oil was monitored following exposure to blue light at 412 nm and 450 nm or cigarette smoke. The anti-aging effect of sandalwood oil was also explored in human skin explants via the assessment of collagenase level (MMP-1). We reported that Indian sandalwood oil possessed antioxidant potential that can scavenge the ROS generated by a free radical generating compound (AAPH). Subsequent exposure to environmental stressors revealed that Indian sandalwood oil possessed superior antioxidant activity in comparison to vitamin E (alpha tocopherol). Using human skin explants, this study demonstrated that Indian sandalwood oil can also inhibit the pollutant-induced level of MMP-1. The findings indicated that Indian sandalwood oil can potentially serve as a protective and anti-aging active ingredient in cosmetics and dermatology against environmental stressors.

scholarly journals Kinetic Cytokine Secretion Profile of LPS-Induced Inflammation in the Human Skin Organ Culture

Pharmaceutics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 299 ◽  
Author(s):  
Raanan Gvirtz ◽  
Navit Ogen-Shtern ◽  
Guy Cohen
Keyword(s):  
Organ Culture ◽  
Human Skin ◽  
Comprehensive Evaluation ◽  
Ex Vivo ◽  
Cytokine Secretion ◽  
Peak Time ◽  
Local Skin ◽  
The Impact ◽  
Skin Organ Culture

Several in vitro models that mimic different aspects of local skin inflammation exist. The use of ex vivo human skin organ culture (HSOC) has been reported previously. However, comprehensive evaluation of the cytokine secretory capacity of the system and its kinetics has not been performed. Objective: the aim of the current study was to investigate the levels and secretion pattern of key cytokine from human skin tissue upon lipopolysaccharide (LPS) stimulation. HSOC maintained in an air–liquid interface was used. Epidermal and tissue viability was monitored by MTT and Lactate Dehydrogenase (LDH) activity assay, respectively. Cytokine levels were examined by ELISA and multiplex array. HSOCs were treated without or with three different LPS subtypes and the impact on IL-6 and IL-8 secretion was evaluated. The compounds enhanced the secreted levels of both cytokines. However, differences were observed in their efficacy and potency. Next, a kinetic multiplex analysis was performed on LPS-stimulated explants taken from three different donors to evaluate the cytokine secretion pattern during 0–72 h post-induction. The results revealed that the pro-inflammatory cytokines IL-6, IL-8, TNFα and IL-1β were up-regulated by LPS stimuli. IL-10, an anti-inflammatory cytokine, was also induced by LPS, but exhibited a different secretion pattern, peak time and maximal stimulation values. IL-1α and IL-15 showed donor-specific changes. Lastly, dexamethasone attenuated cytokine secretion in five independent repetitions, supporting the ability of the system to be used for drug screening. The collective results demonstrate that several cytokines can be used as valid inflammatory markers, regardless of changes in the secretion levels due to donor’s specific alterations.

scholarly journals Nanostructured Lipid Carrier Gel for the Dermal Application of Lidocaine: Comparison of Skin Penetration Testing Methods

Pharmaceutics ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 310 ◽  
Author(s):  
Stella Zsikó ◽  
Kendra Cutcher ◽  
Anita Kovács ◽  
Mária Budai-Szűcs ◽  
Attila Gácsi ◽  
...  
Keyword(s):  
Drug Release ◽  
Human Skin ◽  
Ex Vivo ◽  
Study Drug ◽  
Skin Penetration ◽  
Human Epidermis ◽  
Nanostructured Lipid Carrier ◽  
Experimental Findings

The aim of this research was to investigate the stability of a lidocaine-loaded nanostructured lipid carrier dispersion at different temperatures, formulate a nanostructured lipid carrier gel, and test the penetration profile of lidocaine from the nanostructured lipid carrier gel using different skin penetration modeling methods. The formulations were characterized by laser diffraction, rheological measurements and microscopic examinations. Various in vitro methods were used to study drug release, diffusion and penetration. Two types of vertical Franz diffusion cells with three different membranes, including cellulose, Strat-M®, and heat separated human epidermis were used and compared to the Skin-parallel artificial membrane permeability assay (PAMPA) method. Results indicated that the nanostructured lipid carrier dispersion had to be gelified as soon as possible for proper stability. Both the Skin-PAMPA model and Strat-M® membranes correlated favorably with heat separated human epidermis in this research, with the Strat-M® membranes sharing the most similar drug permeability profile to an ex vivo human skin model. Our experimental findings suggest that even when the best available in vitro experiment is selected for modeling human skin penetration to study nanostructured lipid carrier gel systems, relevant in vitro/in vivo correlation should be made to calculate the drug release/permeation in vivo. Future investigations in this field are still needed to demonstrate the influence of membranes and equipment from other classes on other drug candidates.

Assessment of the biochemical effects of percutaneous exposure of sulphur mustard in an in vitro human skin system

1996 ◽  
Vol 15 (3) ◽  
pp. 237-244 ◽  
Author(s):  
CD Lindsay ◽  
P. Rice
Keyword(s):  
Inflammatory Response ◽  
Human Skin ◽  
Serine Proteases ◽  
Inflammatory Cells ◽  
Chemical Warfare Agent ◽  
Skin Damage ◽  
Sulphur Mustard ◽  
Nuclear Pyknosis ◽  
Skin Explants

1 Sulphur mustard (HD) is a potent chemical warfare agent which causes incapacitating blisters on human skin. There is no specific pretreatment nor therapy against this agent and the mechanism of dermo-epidermal cleavage is unclear. The aim of this study was to use a human skin explant system to determine the consequences of percuta neous exposure to HD. 2 Increased activities of serine proteases associated with blistering disorders in humans were detected from human skin explants after exposure to HD. The most consistent response and the highest protease activities measured were found for trypsin. This class of enzyme is therefore implicated in the dermo-epidermal separation which is associated with blistering in humans following exposure to HD. 3 An inflammatory response was observed in the skin explants exposed to HD. At low doses of HD it was characterised by the presence of neutrophils in the papillary dermis, culminating in the infiltration of the epidermis by these inflammatory cells at higher concen trations of HD. A variety of other histopathological changes in the explants was found such as focal dermo- epidermal separation, nuclear pyknosis and perinuclear vacuolation. 4 The study indicates that full thickness human skin explants can be used to investigate various aspects of the possible pathogenesis of HD-induced skin damage, in cluding the associated inflammatory response.

Cutaneous DNA delivery and gene expression in ex vivo human skin explants via wet-etch microfabricated microneedles

2005 ◽  
Vol 13 (7) ◽  
pp. 415-421 ◽  
Author(s):  
James Birchall ◽  
Sion Coulman ◽  
Marc Pearton ◽  
Chris Allender ◽  
Keith Brain ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Skin ◽  
Ex Vivo ◽  
Dna Delivery ◽  
Skin Explants ◽  
Wet Etch

scholarly journals Delivering nitric oxide into human skin from encapsulated S-nitrosoglutathione under UV light: An in vitro and ex vivo study

Nitric Oxide ◽  
2020 ◽  
Vol 94 ◽  
pp. 108-113 ◽  
Author(s):  
Milena T. Pelegrino ◽  
Richard B. Weller ◽  
André Paganotti ◽  
Amedea B. Seabra
Keyword(s):  
Nitric Oxide ◽  
Human Skin ◽  
Uv Light ◽  
Ex Vivo ◽  
Ex Vivo Study

scholarly journals Effect of Skin Model on In Vitro Performance of an Adhesive Dermally Applied Microarray Coated with Zolmitriptan

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Mahmoud Ameri ◽  
Hayley Lewis ◽  
Paul Lehman
Keyword(s):  
Human Skin ◽  
Ex Vivo ◽  
Full Thickness ◽  
Artificial Membrane ◽  
Skin Model ◽  
Hydrophobic Membrane ◽  
Thickness Skin ◽  
In Vitro Performance

Franz cell studies, utilizing different human skin and an artificial membrane, evaluating the influence of skin model on permeation of zolmitriptan coated on an array of titanium microprojections, were evaluated. Full thickness and dermatomed ex vivo human skin, as well as a synthetic hydrophobic membrane (Strat-M®), were assessed. It was found that the choice of model demonstrated different absorption kinetics for the permeation of zolmitriptan. For the synthetic membrane only 11% of the zolmitriptan coated dose permeated into the receptor media, whilst for the dermatomed skin 85% permeated into the receptor. The permeation of zolmitriptan through full thickness skin had a significantly different absorption profile and time to maximum flux in comparison to the dermatomed skin and synthetic model. On the basis of these results dermatomed skin may be a better estimate of in vivo performance of drug-coated metallic microprojections.

Sign in / Sign up

Export Citation Format

Share Document