scholarly journals Broussonetia papyrifera Promotes Hair Growth Through the Regulation of β-Catenin and STAT6 Target Proteins: A Phototrichogram Analysis of Clinical Samples

Cosmetics ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 40
Author(s):  
Young Han Lee ◽  
Gaewon Nam ◽  
Myong-Ki Kim ◽  
Seok-Cheol Cho ◽  
Bu Young Choi
Keyword(s):  
Hair Growth ◽  
Dermal Papilla ◽  
Interleukin 4 ◽  
Clinical Samples ◽  
Broussonetia Papyrifera ◽  
Dermal Papilla Cells ◽  
Target Proteins ◽  
Start Date ◽  
Growth Assay

Broussonetia papyrifera (B.papyrifera), belonging to the Moraceae family, is known to elicit anti-inflammatory, antioxidant, anti-tyrosinase, anticancer, antinociceptive, and antimicrobial effects. The present study has been designed to examine the effects of B. papyrifera extract on hair growth through in vitro and clinical samples. Real-time cell growth assay, T-cell factor/lymphoid enhancer-binding factor (TCF/LEF), activation of signal transducer and activator of transcription-6(STAT6) and STAT3 reporter gene function, and Western blotting was performed to examine whether B. papyrifera regulates the expression of target proteins implicated in the proliferation of human hair follicle dermal papilla (hHFDP) cells. In this human trial, using a phototrichogram, the effect of B. papyrifera on hair growth was examined by reconstitution analysis after shaving the hair of the clinical subject’s dorsal skin. B. papyrifera promoted growth equally in hHFDP cells, which is comparable to that of minoxidil and tofacitinib. Treatment with B. papyrifera extract enhanced the TCF/LEF-luciferase activity and increased the level of β-catenin protein. Moreover, B. papyrifera extract significantly suppressed interleukin-4 (IL4)-induced STAT6 phosphorylation. In clinical trial, using a phototrichogram, we assessed the hair density and total hair counts at 0, 6, and 12 weeks after the use of hair tonic containing B. papyrifera extract. After using the hair tonic for 12 weeks, the total hair count was significantly increased as compared with the subjects at the start date (n = 11). B. papyrifera promotes dermal papilla cells proliferation in vitro and clinically among human volunteers through the regulation of WNT-β-catenin and STAT6 pathways.

scholarly journals Preclinical and Clinical Studies Demonstrate That the Proprietary Herbal Extract DA-5512 Effectively Stimulates Hair Growth and Promotes Hair Health

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Jae Young Yu ◽  
Biki Gupta ◽  
Hyoung Geun Park ◽  
Miwon Son ◽  
Joon-Ho Jun ◽  
...  
Keyword(s):  
Clinical Study ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Herbal Extract ◽  
Control Group ◽  
Dependent Manner ◽  
Ki 67 ◽  
Dermal Papilla Cells

The proprietary DA-5512 formulation comprises six herbal extracts from traditional oriental plants historically associated with therapeutic and other applications related to hair. Here, we investigated the effects of DA-5512 on the proliferation of human dermal papilla cells (hDPCs) in vitro and on hair growth in C57BL/6 mice and conducted a clinical study to evaluate the efficacy and safety of DA-5512. DA-5512 significantly enhanced the viability of hDPCs in a dose-dependent manner (p<0.05), and 100 ppm of DA-5512 and 1 μM minoxidil (MXD) significantly increased the number of Ki-67-positive cells, compared with the control group (p<0.05). MXD (3%) and DA-5512 (1%, 5%) significantly stimulated hair growth and increased the number and length of hair follicles (HFs) versus the controls (each p<0.05). The groups treated with DA-5512 exhibited hair growth comparable to that induced by MXD. In clinical study, we detected a statistically significant increase in the efficacy of DA-5512 after 16 weeks compared with the groups treated with placebo or 3% MXD (p<0.05). In conclusion, DA-5512 might promote hair growth and enhance hair health and can therefore be considered an effective option for treating hair loss.

scholarly journals Nature-derived lignan compound VB-1 exerts hair growth-promoting effects by augmenting Wnt/β-catenin signaling in human dermal papilla cells

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4737 ◽  
Author(s):  
Jieshu Luo ◽  
Mengting Chen ◽  
Yingzi Liu ◽  
Hongfu Xie ◽  
Jian Yuan ◽  
...  
Keyword(s):  
Hair Growth ◽  
Dermal Papilla ◽  
Hair Shaft ◽  
Cell Counting ◽  
Dependent Manner ◽  
Rt Pcr ◽  
Concentration Dependent Manner ◽  
Dermal Papilla Cells ◽  
Growth Promoting

Background Vitexin is a kind of lignan compound which has been shown to possess a variety of pharmacological effects, such as anti-inflammatory, anti-oxidative and anti-cancer activities. However the effect of vitexin on hair regeneration has not been elaborated. Methods The proliferation of human dermal papilla cells (hDPCs) was examined by cell counting and continuous cell culture after vitexin compound 1 (VB-1) was treated. The expression of lef1, wnt5a, bmp2, bmp4, alpl and vcan was examined by RT-PCR. The expression of dkk1, tgf-β1, active-β-Catenin, and AXIN2 was examined by RT-PCR or immunoblotting. Hair shaft growth was measured in the absence or presence of VB-1. Results We demonstrated that VB-1 significantly promotes the proliferation of hDPCs in a concentration-dependent manner within a certain concentration range. Among the hair growth-related genes investigated, dkk1 was clearly down-regulated in hDPCs treated with VB-1. The increased active β-Catenin and decreased AXIN2 protein levels suggest that VB-1 facilitates Wnt/β-catenin signaling in hDPCs in vitro. The expression of DP signature genes was also upregulated after VB-1 treatment. Our study further indicated that VB-1 promotes human hair follicle (HF) growth by HF organ culture assay. Discussion VB-1 may exert hair growth-promoting effects via augmenting Wnt/β-catenin signaling in hDPCs.

scholarly journals Establishment of 3D Spheroid Human Dermal Papilla Cells as an Effective Model for Hair Growth Screening Compounds Compared with the 2D System: An Example of Minoxidil and 3,4,5-Tri-O-caffeoylquinic acid (TCQA)

2021 ◽  
Author(s):  
Meriem Bejaoui ◽  
Aprill Kee Oliva ◽  
May Sin Ke ◽  
Farhana Ferdousi ◽  
Hiroko Isoda
Keyword(s):  
Hair Follicle ◽  
Hair Growth ◽  
In Vitro Model ◽  
3D Model ◽  
Dermal Papilla ◽  
Dermal Papilla Cells ◽  
Caffeoylquinic Acid ◽  
Vitro Model

Abstract IntroductionDermal papilla cells (DPc) is an important element in studying the hair follicle (HF) niche. The human hair follicle dermal papilla cells (HFDPC) are widely used as an in vitro model to study hair growth related research. These cells are usually grown in 2D culture, nevertheless, this system did not show efficient therapeutic effect on HF regeneration and growth, and key differences were observed between cell activity in vitro and in vivo. ObjectiveRecent studies have showed that HFDPC grown in 3D hanging spheroids is more morphologically akin to intact DPc microenvironment. This current study showed that the 3D model is applicable to the commercial cell line with new insights on its variability by comparing to previous studies of gene signature restored by 3D culture.Methods and Results Our data demonstrated that HFDPCS grown in 3D in vitro model can influence not only hair growth-related pathways but also immune system -related pathways compared to 2D cell monolayer. Furthermore, we compared the expression of signalling molecules and metabolism-associated proteins of HFDPC in minoxidil (FDA approved drug for hair loss treatment) and 3,4,5-tri-O-caffeoylquinic acid (TCQA) (recently found to induce hair growth in vitro and in vivo) treated 3D and 2D cell cultures using microarray analysis. Conclusion Further validation of the results confirms the suitability of this cell line for 3D model while providing new insights such as to the mechanisms behind the hair growth effects of 3D spheroid treated with hair growth promoting agents.

scholarly journals In Vitro Hair Dermal Papilla Cells Induction by Fagraea berteroana, a Tree of the Marquesan Cosmetopoeia (French Polynesia)

Cosmetics ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 13
Author(s):  
Kristelle Hughes ◽  
Raimana Ho ◽  
Claire Chazaud ◽  
Stéphanie Hermitte ◽  
Stéphane Greff ◽  
...  
Keyword(s):  
Transforming Growth Factor Beta ◽  
Hair Growth ◽  
Transforming Growth Factor ◽  
Dermal Papilla ◽  
Fold Increase ◽  
French Polynesia ◽  
Bone Morphogenetic Protein 2 ◽  
Dermal Papilla Cells ◽  
Growth State

Fagraea berteroana is a tree used in traditional medicine in various islands of the South Pacific. Here, we studied its hair growth-inducing properties as suggested by one of its Marquesan ethno-uses in haircare. The ethyl acetate extract of the fruits of F. berteroana (FEAE) and four resulting fractions (FEAE-F0, FEAE-F1, FEAE-F2, and FEAE-F3) were tested on hair follicle dermal papilla cells to determine their cell proliferative activity. Furthermore, RT-qPCR analysis enabled gene modulation analysis, while immunostaining of the β-catenin protein was used to follow protein regulation. We found that the plant extracts induced a controlled, dose-dependent cell proliferation. FEAE-F0 simultaneously down-regulated Bone Morphogenetic Protein 2 (BMP2) mRNA expression and upregulated Cyclin-D1 (CCND1) gene expression, which suggests an involvement in the regulation of the Wnt and Transforming Growth Factor beta (TGFβ) pathways that control the hair cycle. FEAE-F0 exhibited a 1.34-fold increase of nuclear β-catenin protein. This is indicative of an active hair growth state. Thus, we conclude that FEAE-F0 could be an innovative candidate in hair care, which opens interesting leads to promote the Marquesan cosmetopoeia.

scholarly journals In Vitro Hair Growth Promoting Effect of a Noncrosslinked Hyaluronic Acid in Human Dermal Papilla Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Nicola Zerbinati ◽  
Sabrina Sommatis ◽  
Cristina Maccario ◽  
Maria C. Capillo ◽  
Serena Di Francesco ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
In Vitro Assays ◽  
Promoting Effect ◽  
Dermal Papilla Cells ◽  
Clinical Investigations ◽  
Endothelial Growth Factor

Dermal papilla cells (DPCs) are a source of nutrients and growth factors, which support the proliferation and growth of keratinocytes as well as promoting the induction of new hair follicles and maintenance of hair growth. The protection from reactive oxygen species (ROS) and the promotion of angiogenesis are considered two of the basal mechanisms to preserve the growth of the hair follicle. In this study, a noncrosslinked hyaluronic acid (HA) filler (HYDRO DELUXE BIO, Matex Lab S.p.A.) containing several amino acids was tested with in vitro assays on human follicle dermal papilla cells (HFDPCs). The experiments were carried out to investigate the possible protection against oxidative stress and the ability to increase the vascular endothelial growth factor (VEGF) release. The results demonstrated the restoration of cell viability against UVB-induced cytotoxicity and an increase in the VEGF secretion. These data demonstrate the capability of the product to modulate human dermal papilla cells, suggesting a future use in mesotherapy, a minimally invasive local intradermal therapy (LIT), after further clinical investigations.

scholarly journals Dermal Papilla Cells and Melanocytes Response to Physiological Oxygen Levels Depends on Their Interactions 

2020 ◽  
Author(s):  
Carla Abreu ◽  
Rui L Reis ◽  
Alexandra P Marques
Keyword(s):  
Hair Growth ◽  
Cultured Cells ◽  
Dermal Papilla ◽  
Tyrosinase Activity ◽  
Ros Production ◽  
Dermal Papilla Cells ◽  
Biologically Relevant ◽  
Oxygen Levels ◽  
Alp Activity

Abstract Background: The specialized dermal papilla (DP) cells and the tyrosinase-active melanocytes are central players in hair growth and pigmentation, respectively. In the hair follicle (HF), oxygen levels average about 5%O2 (physoxia) and are intimately coupled with the production of reactive oxygen species (ROS), which contribute to hair growth. Considering that oxygen and ROS might have a major role in the maintenance of the HF cellular functions, we studied the effect of physoxia over human DP cells and melanocytes (hMel) and investigated if these cells interaction altered this response.Results: Physoxia decreased DP cells senescence and improved their secretome and phenotype, representing a reliable in vitro culture environment. Further, hMel proliferation, migration and tyrosinase activity were also enhanced, demonstrating physoxia capacity to sustain biologically relevant features. Physoxia affected DP cells alkaline phosphatase (ALP) activity, but their signalling did not influence hMel proliferation or tyrosinase activity when indirectly co-cultured. Additionally, ROS production in co-cultured cells was higher than in the respective monocultures, but lower in response to physoxia than in normoxia (21%O2). Considering the results, we further assessed a potential link between DP cells ALP activity and ROS levels, but DP cells treatment with H2O2 showed that these are not directly correlated. Moreover, and given their proximity within the HF, hMel were directly cultured with DP spheroids, rendering 3D-aggregates that recreated these cells native microarchitecture and phenotype. Further, both hMel tyrosinase activity and DP cells ALP activity, their main functional indicators, as well as ROS production were higher in the 3D-aggregates cultured under physoxia than in normoxia. Conclusions: Overall, we provide evidence that the response to physoxia differs according to hMel-DP cells interactions, and that the microenvironment recreated when they are in direct contact favours their follicular functions, including hair growth and pigmentation promoting capacity.

scholarly journals In Vitro Assessment of Biofield Energy Healing Treatment on Hair Growth by Enhanced Proliferation of Human Follicular Dermal Papilla Cells (HFDPCs)

2018 ◽  
Vol 4 (1) ◽  
pp. 30-44
Author(s):  
Dahryn Trivedi ◽  
Snehasis Jana
Keyword(s):  
Hair Growth ◽  
Dermal Papilla ◽  
Growth Promoter ◽  
Androgenic Alopecia ◽  
Dermal Papilla Cells ◽  
Test Items ◽  
Energy Healing ◽  
The One

Patterned hair loss or androgenic alopecia is a disorder affecting millions of peoples, in which hair sheds without adequate regrowth. Authors propose a new approach to increase hair growth. In this study, authors examined the in vitro potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test items, Dulbecco’s Modified Eagle Medium (DMEM) on the Human Follicular Dermal Papilla Culture Cells (HFDPC) for the assessment of growth and development. The test items (DMEM) were divided into three parts. First part did not receive any sort of treatment and defined as the untreated DMEM group. The second and third parts were treated with the one-time and two-times Biofield Energy Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and coded as the one-time Biofield Energy Treated DMEM (BT-I) and two-times Biofield Energy Treated DMEM (BT-II) groups, respectively. The Biofield Energy Healing Treatment was provided by a renowned Biofield Energy Healer (The Trivedi Effect®), remotely for ~5 minutes under standard laboratory conditions through unique energy transmission process. The experimental results showed that one-time Biofield Energy Treated DMEM showed 70.24% and two-times Biofield Energy Treated DMEM significantly (p ≤ 0.001) increased the proliferation of dermal papilla cells by 207.62% as compared to the untreated DMEM group. The overall results demonstrated that the Biofield Energy Healing Treatment significantly increased the proliferation of human hair follicle dermal papilla cells in vitro. Therefore, the Consciousness Energy Healing (The Trivedi Effect®) Treatment might be useful as a hair growth promoter in vivo against different types of skin injuries, hair disorders, and opens new research venues.

Effects of glucocorticoid on human dermal papilla cells in vitro

2013 ◽  
Vol 135 ◽  
pp. 24-29 ◽  
Author(s):  
Soon-Jin Choi ◽  
A-Ri Cho ◽  
Seong-Jin Jo ◽  
Sungjoo Tommy Hwang ◽  
Kyu Han Kim ◽  
...  
Keyword(s):  
Dermal Papilla ◽  
Dermal Papilla Cells
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