scholarly journals Characterization of Soluplus/ASC-DP Nanoparticles Encapsulated with Minoxidil for Skin Targeting

2021 ◽  
Vol 5 (3) ◽  
pp. 44
Author(s):  
Rina Takayama ◽  
Moe Ishizawa ◽  
Miyuki Yamada ◽  
Yutaka Inoue ◽  
Ikuo Kanamoto
Keyword(s):  
Hydrophobic Interactions ◽  
Skin Penetration ◽  
Drug Carriers ◽  
Skin Permeability ◽  
Particle Structure ◽  
Spherical Micelle ◽  
Transmission Electron ◽  
Skin Targeting ◽  
1H Nuclear Magnetic Resonance ◽  
Poorly Soluble

Soluplus (Sol) is an amphiphilic graft copolymer capable of forming self-assembled micelles and L-ascorbyl 2,6-dipalmitate (ASC-DP) aggregates spontaneously to form micelles. Micelles are used as drug carriers and can nanoparticulate drugs that are poorly soluble in water, such as minoxidil. The study aimed to prepare minoxidil-encapsulated nanoparticles using Sol/ASC-DP and evaluate their potential for targeted skin application. Sol/ASC-DP nanoparticles or Sol/ASC-DP with minoxidil were prepared using the hydration method, and physical evaluations were carried out, including assessments of particle size and zeta potential. Particle structure was evaluated by transmission electron microscopy (TEM) and 1H-nuclear magnetic resonance spectra to assess particle stability and perform functional evaluations in skin penetration tests. TEM images showed spherical micelle-like particles of approximately 100 nm for Sol/ASC-DP at a 9:1 ratio and of approximately 80 nm for Sol/ASC-DP with incorporated minoxidil at a 9:1:0.5 ratio. Changes were also observed in the solid state, suggesting a hydrophobic interaction between Sol and ASC-DP. In addition, evaporated microparticles (Sol/ASC-DP/minoxidil = 9/1/0.5) improved the skin permeability of minoxidil. These results suggest that Sol/ASC-DP nanoparticles form a stable new nanoparticle due to hydrophobic interactions, which would improve the skin permeability of minoxidil.

scholarly journals Evaluating biological activity of folic acid-modified and 10-hydroxycamptothecin-loaded mesoporous silica nanoparticles

2021 ◽  
Author(s):  
Mei-Xia Zhao ◽  
Di-Feng Chen ◽  
Xue-Jie Zhao ◽  
Lin-Song Li ◽  
Yong-Fang Liu
Keyword(s):  
Folic Acid ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Folate Receptor ◽  
Mesoporous Silica Nanoparticles ◽  
Drug Carriers ◽  
Transmission Electron ◽  
Before And After ◽  
Drug Efficiency

Targeted nanocarrier can selectively deliver anti-tumor drugs to cancer sites improving drug efficiency. Accordingly, a targeted nanocarrier (MSN-FA) was synthesized based on folic acid (FA) modified mesoporous silica nanoparticles (MSNs). These loaded with 10-hydroxycamptothecin (HCPT) to obtain the nano-drug MSN-FA@HCPT. These nanocarriers were characterized by transmission electron microscopy (TEM), zeta potential, ultraviolet-visible spectroscopy (UV-Vis), fourier transform infrared spectroscopy (FT-IR), and thermogravimetric analysis (TGA). Notably, the nanocarriers were nearly spherical before and after loading HCPT and exhibited good dispersibility. Also, folate receptor (FR) over-expressing HeLa cells and FR deficient HepG2 cells were used to evaluate in vitro cellular uptake and cytotoxicity of MSN-FA@HCPT and MSN@HCPT. Interestingly, FA-modified nanocarriers enhanced the cytotoxicity of HCPT by improving drug targeting to tumor cells. Also, apoptotic and mitochondrial membrane potential (MMP) reducing effects of MSN-FA@HCPT were more prominent than the MSNs without FA modification. MSN-FA@HCPT can be excellent drug carriers with profound biomedical applications.

scholarly journals In Vitro Anticancer Properties of Copper Metallodendrimers

Biomolecules ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 155 ◽  
Author(s):  
Hołota ◽  
Magiera ◽  
Michlewska ◽  
Kubczak ◽  
del Olmo ◽  
...  
Keyword(s):  
Fluorescence Anisotropy ◽  
Drug Carriers ◽  
Haemolytic Activity ◽  
Surface Groups ◽  
Biological Processes ◽  
Normal Cells ◽  
Anticancer Properties ◽  
Transmission Electron ◽  
Anti Cancer

Newly synthesized carbosilane copper dendrimers (CCD) with chloride and nitrate surface groups seem to be good candidates to be used as gene and drug carriers in anti-cancer therapy, due to their properties such as size and surface charge. Copper attached to the nanoparticles is an important element of many biological processes and recently their anti-cancer properties have been widely examined. Zeta size and potential, transmission electron microscopy (TEM), circular dichroism (CD), analysis of haemolytic activity, and fluorescence anisotropy techniques were used to characterize copper dendrimers. Additionally, their cytotoxic properties toward normal (PBMC) and cancer (1301; HL-60) cells were examined. All tested dendrimers were more cytotoxic against cancer cells in comparison with normal cells.

scholarly journals Transmission Electron Microscopy of a Poorly Soluble Drug, Felodipine

2016 ◽  
pp. 720-721
Author(s):  
Mark S'ari ◽  
James Cattle ◽  
Andy Brown ◽  
Brydson Rik ◽  
Nicole Hondow ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Transmission Electron ◽  
Poorly Soluble ◽  
Poorly Soluble Drug

scholarly journals Effect of Hydrophobic Interactions on Lower Critical Solution Temperature for Poly(N-isopropylacrylamide-co-dopamine Methacrylamide) Copolymers

Polymers ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 991 ◽  
Author(s):  
Alberto García-Peñas ◽  
Chandra Sekhar Biswas ◽  
Weijun Liang ◽  
Yu Wang ◽  
Pianpian Yang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Lower Critical Solution Temperature ◽  
Hydrophobic Interactions ◽  
Structural Information ◽  
Drug Carriers ◽  
Solution Temperature ◽  
Polymerization Degree ◽  
Tissue Engineering Scaffolds ◽  
Critical Solution Temperature ◽  
Precise Control

For the preparation of thermoresponsive copolymers, for e.g., tissue engineering scaffolds or drug carriers, a precise control of the synthesis parameters to set the lower critical solution temperature (LCST) is required. However, the correlations between molecular parameters and LCST are partially unknown and, furthermore, LCST is defined as an exact temperature, which oversimplifies the real situation. Here, random N-isopropylacrylamide (NIPAM)/dopamine methacrylamide (DMA) copolymers were prepared under a systematical variation of molecular weight and comonomer amount and their LCST in water studied by calorimetry, turbidimetry, and rheology. Structural information was deduced from observed transitions clarifying the contributions of molecular weight, comonomer content, end-group effect or polymerization degree on LCST, which were then statistically modeled. This proved that the LCST can be predicted through molecular structure and conditions of the solutions. While the hydrophobic DMA lowers the LCST especially the onset, polymerization degree has an important but smaller influence over all the whole LCST range.

scholarly journals Dermal Delivery of the High-Molecular-Weight Drug Tacrolimus by Means of Polyglycerol-Based Nanogels

Pharmaceutics ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 394 ◽  
Author(s):  
Fiorenza Rancan ◽  
Hildburg Volkmann ◽  
Michael Giulbudagian ◽  
Fabian Schumacher ◽  
Jessica Isolde Stanko ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Skin Barrier ◽  
Skin Penetration ◽  
Jurkat Cells ◽  
Skin Permeability ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hydration Properties ◽  
Tacrolimus Ointment ◽  
Proliferative Effect ◽  
Inflammatory Conditions

Polyglycerol-based thermoresponsive nanogels (tNGs) have been shown to have excellent skin hydration properties and to be valuable delivery systems for sustained release of drugs into skin. In this study, we compared the skin penetration of tacrolimus formulated in tNGs with a commercial 0.1% tacrolimus ointment. The penetration of the drug was investigated in ex vivo abdominal and breast skin, while different methods for skin barrier disruption were investigated to improve skin permeability or simulate inflammatory conditions with compromised skin barrier. The amount of penetrated tacrolimus was measured in skin extracts by liquid chromatography tandem-mass spectrometry (LC-MS/MS), whereas the inflammatory markers IL-6 and IL-8 were detected by enzyme-linked immunosorbent assay (ELISA). Higher amounts of tacrolimus penetrated in breast as compared to abdominal skin or in barrier-disrupted as compared to intact skin, confirming that the stratum corneum is the main barrier for tacrolimus skin penetration. The anti-proliferative effect of the penetrated drug was measured in skin tissue/Jurkat cells co-cultures. Interestingly, tNGs exhibited similar anti-proliferative effects as the 0.1% tacrolimus ointment. We conclude that polyglycerol-based nanogels represent an interesting alternative to paraffin-based formulations for the treatment of inflammatory skin conditions.

Study on the Preparation, Property and Drug Release Carrier of Chitosan-Based Nanoparticles for Cancer Treatment

2011 ◽  
Vol 284-286 ◽  
pp. 1800-1803
Author(s):  
Chih Wei Chou ◽  
Yueh Hsiung Kuo ◽  
You Cheng Hseu ◽  
Yung Shiu Chen ◽  
Te Hsing Wu
Keyword(s):  
Electron Microscope ◽  
Near Infrared ◽  
Ultra Violet ◽  
Drug Carriers ◽  
Therapeutic Agents ◽  
Synthetic Method ◽  
Grafting Reaction ◽  
Transmission Electron ◽  
Infrared Spectrophotometer ◽  
Ft Ir

In this paper, the effective nanocompoties (EFNCs) as drug carriers are prepared by grafting reaction of cationic chitosan (CS) and anionic tripolyphosphate (TPP) to encapsulate cancer therapeutic agents such as berberine (BB), KCY-24B…etc. These EFNCs were featured by ultra violet–visible-near infrared spectrophotometer (UV-VIS-NIR spectrophotometer), fourier transform infrared spectrophotometer (FT-IR), zetasizer, transmission electron microscope (TEM) and field-emission scanning electron microscope (FESEM). So we can obtain here the synthetic method, characterization, and functionality of these effective nanoparticles.

scholarly journals OPTIMIZATION OF SELF-NANOEMULSIFYING DRUG DELIVERY SYSTEMS OF LEMONGRASS (CYMBOPOGON CITRATUS) ESSENTIAL OIL

2019 ◽  
Vol 11 (1) ◽  
pp. 144
Author(s):  
Tri Ujilestari ◽  
Bambang Ariyadi ◽  
Ronny Martien ◽  
Zuprizal . ◽  
Nanung Danar Dono
Keyword(s):  
Drug Delivery ◽  
Essential Oil ◽  
Coconut Oil ◽  
Tween 80 ◽  
Cymbopogon Citratus ◽  
Peg 400 ◽  
Transmission Electron ◽  
Poorly Soluble ◽  
Optimum Formula ◽  
Mixture Experimental Design

Objective: Focus of this study was to optimize and to characterize the self-Nano emulsifying drug delivery system using lemongrass (Cymbopogon citratus) essential oil.Methods: The optimum formulas were analyzed using a D-Optimal mixture experimental design and performed using a Design Expert® Ver. 7.1.5. Formulation variables which include in the design were: oil component X1 (a mixture of Cymbopogon citratus essential oil and virgin coconut oil/VCO), surfactant X2 (Tween 80), and co-surfactant (PEG 400), while emulsification time in a sec (Y1) and transmittance in percent (Y2) as responses.Results: The optimum formula for SNEDDS in the current study were: Cymbopogon citratus essential oil (7.147%), VCO (7.147%), Tween 80 (71.417%), and PEG 400 (14.290%). From the optimizing formula can be shown that the mean of droplet size, polydispersity-index, zeta potential, and viscosity were: 13.17±0.06 nm, 0.17±0.05,-20.90±1.47 mV, 200±0mPa. s (n=3), respectively. Furthermore, the optimized formula has passed the thermodynamic stability test; meanwhile, transmission electron microscopy displayed spherical shape.Conclusion: The optimized SNEDDS formula was improving solubility of poorly soluble Cymbopogon citratus essential oil.

scholarly journals Polyfluorene-Based Multicolor Fluorescent Nanoparticles Activated by Temperature for Bioimaging and Drug Delivery

Nanomaterials ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 1485 ◽  
Author(s):  
Marta Rubio-Camacho ◽  
Yolanda Alacid ◽  
Ricardo Mallavia ◽  
María José Martínez-Tomé ◽  
C. Reyes Mateo
Keyword(s):  
Mammalian Cells ◽  
Photophysical Properties ◽  
Drug Carriers ◽  
Fluorescent Nanoparticles ◽  
Tem Analysis ◽  
Transmission Electron ◽  
Hydrophilic Drug ◽  
Thermosensitive Liposomes ◽  
Size And Morphology ◽  
The Stability

Multifunctional nanoparticles have been attracting growing attention in recent years because of their capability to integrate materials with different features in one entity, which leads them to be considered as the next generation of nanomedicine. In this work, we have taken advantage of the interesting properties of conjugated polyelectrolytes to develop multicolor fluorescent nanoparticles with integrating imaging and therapeutic functionalities. With this end, thermosensitive liposomes were coated with three recently synthesized polyfluorenes: copoly-((9,9-bis(6′-N,N,N-trimethylammonium)hexyl)-2,7-(fluorene)-alt-1,4-(phenylene)) bromide (HTMA-PFP), copoly-((9,9-bis(6′-N,N,N-trimethylammonium)hexyl)-2,7-(fluorene)-alt-4,7-(2- (phenyl)benzo(d) (1,2,3) triazole)) bromide (HTMA-PFBT) and copoly-((9,9-bis(6′-N,N,N- trimethylammonium)hexyl)-2,7-(fluorene)-alt-1,4-(naphtho(2,3c)-1,2,5-thiadiazole)) bromide (HTMA-PFNT), in order to obtain blue, green and red fluorescent drug carriers, respectively. The stability, size and morphology of the nanoparticles, as well as their thermotropic behavior and photophysical properties, have been characterized by Dynamic Light Scattering (DLS), Zeta Potential, transmission electron microscope (TEM) analysis and fluorescence spectroscopy. In addition, the suitability of the nanostructures to carry and release their contents when triggered by hyperthermia has been explored by using carboxyfluorescein as a hydrophilic drug model. Finally, preliminary experiments with mammalian cells demonstrate the capability of the nanoparticles to mark and visualize cells with different colors, evidencing their potential use for imaging and therapeutic applications.

scholarly journals Curcumin-In-Deformable Liposomes-In-Chitosan-Hydrogel as a Novel Wound Dressing

Pharmaceutics ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 8 ◽  
Author(s):  
Selenia Ternullo ◽  
Laura Victoria Schulte Werning ◽  
Ann Mari Holsæter ◽  
Nataša Škalko-Basnet
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Chronic Wound ◽  
Skin Penetration ◽  
The Novel ◽  
Chitosan Hydrogel ◽  
Wound Model ◽  
Skin Delivery ◽  
Poorly Soluble ◽  
Dermal Delivery

A liposomes-in-hydrogel system as an advanced wound dressing for dermal delivery of curcumin was proposed for improved chronic wound therapy. Curcumin, a multitargeting poorly soluble active substance with known beneficial properties for improved wound healing, was incorporated in deformable liposomes to overcome its poor solubility. Chitosan hydrogel served as a vehicle providing superior wound healing properties. The novel system should assure sustained skin delivery of curcumin, and increase its retention at the skin site, utilizing both curcumin and chitosan to improve the therapy outcome. To optimize the properties of the formulation and determine the effect of the liposomal charge on the hydrogel properties, curcumin-containing deformable liposomes (DLs) with neutral (NDLs), cationic (CDLs), and anionic (ADLs) surface properties were incorporated in chitosan hydrogel. The charged DLs affected the hydrogel’s hardness, cohesiveness, and adhesiveness. Importantly, the incorporation of DLs, regardless of their surface charge, in chitosan hydrogel did not decrease the system’s bioadhesion to human skin. Stability testing revealed that the incorporation of CDLs in hydrogel preserved hydrogel´s bioadhesiveness to a higher degree than both NDLs and ADLs. In addition, CDLs-in-hydrogel enabled the most sustained skin penetration of curcumin. The proposed formulation should be further evaluated in a chronic wound model.

An Overview: Nanoparticles

1970 ◽  
Vol 1 (3) ◽  
pp. 215-220
Author(s):  
Jayvadan K. Patel ◽  
Dhaval J. Patel ◽  
Vikram M. Pandya
Keyword(s):  
New Technologies ◽  
Drug Effects ◽  
Drug Carriers ◽  
Clinical Aspects ◽  
Different Types ◽  
Long Time ◽  
Poorly Soluble ◽  
Nanoparticles Characterization ◽  
Pharmacological Screening ◽  
Single Material

Nanoparticles have unique properties as compared to micro or macro particles. Different types of nanoparticulate material use in electronic, magnetic, pharmaceutical, cosmetic, energy, catalytic and materials industries. Nanoparticles can be produced by biological, chemical or physical processes. Several of these processes have been known for a long time, while others are new technologies. Nanoparticles are also used in the medical field to aid in drug delivery and medical imaging. Nanoparticles are solid colloidal drug carriers, typically made of a single material, in which a drug is entrapped, encapsulated or adsorbed onto the surface. In pharmaceutical field, it is estimated that about 40% of newly developed drugs will be poorly soluble in future. The poor solubility even makes it very difficult to perform the pharmacological screening of compounds for potential drug effects. With help of nanoparticle technology, this can be solving. Nanoparticle technology is currently taking prominence in the market. This review presents the production process of nanoparticles, characterization techniques & its clinical aspects

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