scholarly journals Treatment of Grade 3 and 4 Osteoarthritis with Intraoperatively Separated Adipose Tissue-Derived Stromal Vascular Fraction: A Comparative Case Series

Cells ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 2096
Author(s):  
Denis Simunec ◽  
Honey Salari ◽  
Juliane Meyer
Keyword(s):  
Adipose Tissue ◽  
Platelet Rich Plasma ◽  
Subjective Evaluation ◽  
Stromal Vascular Fraction ◽  
Case Series ◽  
Joint Space ◽  
Substantial Improvement ◽  
Serious Adverse Events ◽  
Artificial Joint Replacement ◽  
Grade 3

Osteoarthritis (OA) is the most common form of arthritis of the joints. The stromal vascular fraction (SVF) is a regenerative cell population that can be isolated from adipose tissue. It is the immunomodulatory properties of the stromal vascular fraction that make it a promising candidate for the regenerative treatment of OA. Patients with grade 3 and 4 osteoarthritis were treated with the stromal vascular fraction with and without platelet-rich plasma (PRP) and followed up on their Knee Injury and Osteoarthritis Outcome Score (KOOS) score for 12 months, with MRI and subjective evaluation of the procedure. Magnetic resonance imaging (MRI) revealed a widening of the joint space, a restructuring of the cartilage, and an alleviation of effusions in the treated joints. In three of the four treatment groups, a substantial improvement of the KOOS scores was documented at the 12-month follow-up time point. According to the subjective evaluation, 67% of the patients were satisfied or very satisfied with the procedure and would recommend it to others. No serious adverse events or unwanted side effects related to the SVF treatment were observed or reported. Prior to an invasive artificial joint replacement, the treatment of arthritic knee joints with the intraarticular injection of autologous adipose tissue-derived SVF should be considered a regenerative treatment option.

scholarly journals Adipose tissue derived stromal vascular fraction transplantation can recover spinal cord injury in mice

2016 ◽  
Vol 3 (04) ◽  
pp. 144 ◽  
Author(s):  
Hau Thi-My Lam ◽  
Minh Nguyen-Thu Tran ◽  
Khoa Anh Bui ◽  
Thao Thi-Thu Le ◽  
Khanh Hong-Thien Bui ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Adipose Tissue ◽  
Mouse Model ◽  
Platelet Rich Plasma ◽  
Stromal Vascular Fraction ◽  
Paracrine Factors ◽  
Diabetic Ulcer ◽  
Motor Test ◽  
Cord Injury

Introduction: Stem cell therapy is one of the most promising therapies for degenerative diseases and related injuries. Adipose tissue derived stem cells (ADSCs) exhibit some particular properties such as high production of paracrine factors. Indeed, ADSCs have been successfully used to treat diseases, including osteoarthritis, diabetic ulcer, etc. Methods: In this study, ADSCs were used to treat spinal cord injury (SCI) in a mouse model. Non-expanded ADSCs, from stromal vascular fractions (SVFs) isolated from both autologous and allogeneic adipose tissues, were injected into injured sites of mice at a specified dose. The SCI mouse model were generated by transection of spinal cord at vertebrae T8 - T10. After 1 week of transection, mice exhibiting completed SCI were divided into 4 groups: group 1 was control (mice without any treatment), group 2 was placebo (mice treated with platelet rich plasma (PRP)), group 3 was allogeneic SVF transplantation (mice treated with allogeneic SVFs), and group 4 was autologous SVF transplantation (mice treated with autologous SVFs). For the treatment groups, mice were transplanted with 20 µL of activated PRP or/and with 106 cells of SVF (allogeneic or autologous) into the injured position through laminectomy. The recovery of SCI was evaluated by locomotor test, sensory test and sensory-motor test at 5 weeks after transplantation. The histology of the spinal cord also was checked after 5 weeks. Results: The results showed that in all groups with PRP injected with or without SVFs, the inflammation was efficiently controlled. The glial scar as well as myelin defragmentation were clearly reduced. However, a significant improvement of BBB score was only recorded in mice transplanted with autologous SVFs. Conclusion: The results of our study show that autologous SVF transplantation in combination with PRP can be a promising therapy for SCI.

scholarly journals Cartilage Regeneration in Human with Adipose Tissue-Derived Stem Cells: Current Status in Clinical Implications

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Jaewoo Pak ◽  
Jung Hun Lee ◽  
Wiwi Andralia Kartolo ◽  
Sang Hee Lee
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Platelet Rich Plasma ◽  
Stromal Vascular Fraction ◽  
Cartilage Regeneration ◽  
Clinical Effectiveness ◽  
The Elderly ◽  
Current Status ◽  
Ability To Regenerate ◽  
Underlying Causes

Osteoarthritis (OA) is one of the most common debilitating disorders among the elderly population. At present, there is no definite cure for the underlying causes of OA. However, adipose tissue-derived stem cells (ADSCs) in the form of stromal vascular fraction (SVF) may offer an alternative at this time. ADSCs are one type of mesenchymal stem cells that have been utilized and have demonstrated an ability to regenerate cartilage. ADSCs have been shown to regenerate cartilage in a variety of animal models also. Non-culture-expanded ADSCs, in the form of SVF along with platelet rich plasma (PRP), have recently been used in humans to treat OA and other cartilage abnormalities. These ADSCs have demonstrated effectiveness without any serious side effects. However, due to regulatory issues, only ADSCs in the form of SVF are currently allowed for clinical uses in humans. Culture-expanded ADSCs, although more convenient, require clinical trials for a regulatory approval prior to uses in clinical settings. Here we present a systematic review of currently available clinical studies involving ADSCs in the form of SVF and in the culture-expanded form, with or without PRP, highlighting the clinical effectiveness and safety in treating OA.

scholarly journals Evaluation of using stromal vascular fraction and platelet rich plasma in the treatment of androgenetic alopecia

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
A B Osama ◽  
Elfeky K Ahdallah ◽  
E K M Ali ◽  
N A Mamdouh
Keyword(s):  
Side Effects ◽  
Clinical Improvement ◽  
Platelet Rich Plasma ◽  
Stromal Vascular Fraction ◽  
Androgenetic Alopecia ◽  
Serious Adverse Events ◽  
Terminal Hair ◽  
Significant Difference ◽  
Potential Therapeutic Application ◽  
Fraction Versus

Abstract Background Platelet Rich Plasma (PRP) is based on the release of growth factors stimulating the initiation/extension of anagen phase as well as promoting vascularization, Adipose Derived Stem Cell (AT-ADSCs) treatment through injection of stromal vascular fraction were recently introduced as an alternative potential therapeutic application for hair growth. Purpose This study aims to compare the efficacy, safety and adverse effects of using lipo-aspirate stromal vascular fraction versus Platelet Rich Plasma injection in the treatment of Androgenetic Alopecia (AGA). Patients And Methods Forty randomized patients were treated by PRP, and SVF. Each patient was evaluated. And each lesion was treated by those modalities, patients received three sessions with one month interval for 3 months, follow up after 3 months. Results A highly significant improvement <0.001 in terminal hair count of SVF group evaluated by videodermoscopy assessment of AGA. That were confirmed by highly significant improvement in inter-mediate hair count and mean caliber (<0.001) associated with high incidence of side effects especially headache and erythema. In contrast. PRP group showed significant improvement 0.048 in terminal hair count and non-significant improvement in inter-mediate hair count and of mean caliber with minimum side effects. SVF group showed a significant improvement in terminal hair count than PRP and a highly significant improvement in Inter-mediate hair count. Regard the clinical improvement and photographic assessment, SVF evoked upper hand of clinical improvement than PRP without statistically difference. Also, side effects of SVF showed highly significant pain, headache and erythema but no serious adverse events. Our study suggests that was significant improvement in AGA after PRP and highly significant after SVF therapy, with significant difference of SVF in terminal hair count and highly significant in vellus hair. Both modalities could effectively and safely he used to treat AGA. Conclusion our study suggested that there was significant improvement in AGA after both PRP and highly significant after SVF therapy. The results indicated a significant difference between PRP and SVF regarding terminal hair count and highly significant for vellus hair count, but non-significant difference in hair diameter. Both modalities could effectively and safely be used to treat AGA and there was statistically improvement in AGA after both PRP and SVF therapies.

scholarly journals Application of Regenerative Cell Technologies in the Treatment of Severe Local Radiation Injuries in Victims of Emergency Situations: from the Experience of the Specialists of the Federal Medical Biophysical Center Named after A.I. Burnazyan of the Federal Medical and Biological Agency of Russia

2021 ◽  
pp. 58-64
Author(s):  
T.A. Astrelina ◽  
◽  
A.V. Aksenenko ◽  
I.V. Kobzeva ◽  
V.A. Brunchukov ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stromal Vascular Fraction ◽  
Healing Time ◽  
Radiation Injuries ◽  
Serious Adverse Events ◽  
Emergency Situations ◽  
Satisfactory Condition ◽  
Complex Therapy ◽  
Local Radiation ◽  
Radiation Ulcers

It has been noted that one of the most common types of radiation injuries when a person is exposed to ionizing radiation is radiation burns — severe local radiation injuries. The aim of the study is to apply stromal and vascular fraction of adipose tissue to increase the efficiency of complex therapy for local radiation lesions. Materials and methods of the study. In 2017-2019, 7 patients (all male; mean age — (54.83±9.41) years) with local radiation lesions of the skin — ulcerative-necrotic lesions of the skin and underlying tissues — were treated at the Federal Medical Biophysical Center named after A.I. Burnazyan of FMBA of Russia. For more than 6 months the patients received conventional conservative therapy of local radiation lesions and a single injection of cell suspension of autologous cells of stromal-vascular fraction of adipose tissue — the average number of cells was (60.33×106±64.04). Results of the study and their analysis. All patients had no serious adverse events and reactions associated with the introduction of autologous regenerative cells of adipose tissue. During the whole period of observation after stromal-vascular fraction of adipose tissue was injected, late radiation ulcers remained without signs of inflammation and infiltration. The patients were discharged from the hospital in satisfactory condition. According to the authors, the use of stromal-vascular fraction of adipose tissue in local radiation lesions provides favorable conditions: to increase the effectiveness of complex therapy; to reduce healing time of the wound surface; to regulate and activate immune and reparative processes in the dermis; to restore the damaged vascular network, lost skin without severe scarring changes; to heal and achieve a satisfactory result, decent quality of life of patients.

scholarly journals Autologous stromal vascular fraction cells combined with platelet-rich plasma for androgenic alopecia treatment: a case series

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 408
Author(s):  
Fonny Josh ◽  
Tomie Hermawan Soekamto ◽  
Muhammad Faruk
Keyword(s):  
Platelet Rich Plasma ◽  
Treatment Options ◽  
Stromal Vascular Fraction ◽  
Case Series ◽  
Androgenic Alopecia ◽  
Case Presentation ◽  
Therapeutic Outcomes ◽  
Multipotent Cells ◽  
Combined Use

Background: Stromal vascular fraction cells (SVFs), which can be produced using a mechanical or digestive enzymatic process, are heterogeneous cells with the potential to grow hair in androgenic alopecia patients. Platelet-rich plasma (PRP) is an autologous cell source that is widely used to treat androgenic alopecia. However, the combined use of PRP and SVFs to treat alopecia is rarely reported. Case presentation: This case series describes three cases of androgenic alopecia, including a32-year-old male, a 43-year-old male, and a 65-year-old male. Androgenic alopecia in these patients was treated by injecting the bald area of the scalp with local autologous PRP combined with SVFs. Follow-up was performed 1–3 months after treatment, which showed good results. The hair grew denser, with increased thickness for each strand. The hair pull test revealed that the hair remained intact. Conclusion: The combination of PRP and SVFs affected all cycles of the hair growth process. SVFs are multipotent cells with the potential to become antioxidant, anti-inflammatory, or anti-fibrotic cells. SVFs can regenerate cells that secrete the growth factors, that are essential for angiogenesis, which can improve therapeutic outcomes. This case series will enrich the existing literature by expanding available treatment options for androgenic alopecia.

scholarly journals Platelet Rich STROMA, the Combination of PRP and tSVF and Its Potential Effect on Osteoarthritis of the Knee

2020 ◽  
Vol 10 (14) ◽  
pp. 4691
Author(s):  
Hieronymus P. Stevens ◽  
Joeri van Boxtel ◽  
Robbert van Dijck ◽  
Joris A. van Dongen
Keyword(s):  
Single Injection ◽  
Platelet Rich Plasma ◽  
Joint Stiffness ◽  
Stromal Vascular Fraction ◽  
Case Series ◽  
Visual Analogue Score ◽  
Fixed Time ◽  
Long Term Results ◽  
Osteoarthritis Of The Knee ◽  
Subsequent Decrease

(1) Background: osteoarthritis (OA) of the knee is a degenerative disease accompanied by pain, reduced mobility and subsequent decrease in quality of life. Many studies on OA of the knee have reported that using an intercellular acting-derivate like platelet-rich plasma (PRP) results in a limited effect or none at all. Authors hypothesized that adding tissue-Stromal Vascular Fraction (tSVF) to PRP (Platelet Rich Stroma (PRS)) would reduce pain and improve functionality in osteoarthritis of the knee. (2) Methods: a consecutive case series of fifteen patients (aged 43–75 years) suffering from OA of the knee (Kellgren–Lawrence stage two to three) were treated with a single injection of autologous PRS. tSVF was mechanically isolated by means of the fractionation of adipose tissue (FAT) procedure. Clinical evaluation was done using a visual analogue score (VAS) score, an adapted Western Ontario and McMaster Universities Osteoarthritis index (WOMAC) and Lysholm score at fixed time points: pre-injection as well as three, six and twelve months post injection. (3) Results: VAS and WOMAC scores improved significantly after twelve months (p < 0.01 and p < 0.05). Lysholm instability scores were also improved at twelve months (p > 0.05) in comparison to pre-injection measurements. No complications were seen in any of the patients. One patient was excluded due to a total knee arthroplasty. (4) Conclusions: a single injection with PRS for OA of the knee seems to lead to an improvement of function and simultaneous reduction of pain and joint stiffness for a period of twelve months. Further controlled trials are required to determine the optimal treatment regimen and evaluate long-term results.

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