scholarly journals Feasibility of Mechanical Extrusion to Coat Nanoparticles with Extracellular Vesicle Membranes

Cells ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1797 ◽  
Author(s):  
Jan Van Deun ◽  
Quentin Roux ◽  
Sarah Deville ◽  
Thibaut Van Acker ◽  
Pekka Rappu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Membrane Proteins ◽  
Protein Composition ◽  
Extracellular Vesicle ◽  
Characterization Methods ◽  
Membrane Morphology ◽  
Vesicle Membranes ◽  
Membrane Coating ◽  
Near Future

Biomimetic functionalization to confer stealth and targeting properties to nanoparticles is a field of intense study. Extracellular vesicles (EV), sub-micron delivery vehicles for intercellular communication, have unique characteristics for drug delivery. We investigated the top-down functionalization of gold nanoparticles with extracellular vesicle membranes, including both lipids and associated membrane proteins, through mechanical extrusion. EV surface-exposed membrane proteins were confirmed to help avoid unwanted elimination by macrophages, while improving autologous uptake. EV membrane morphology, protein composition and orientation were found to be unaffected by mechanical extrusion. We implemented complementary EV characterization methods, including transmission- and immune-electron microscopy, and nanoparticle tracking analysis, to verify membrane coating, size and zeta potential of the EV membrane-cloaked nanoparticles. While successful EV membrane coating of the gold nanoparticles resulted in lower macrophage uptake, low yield was found to be a significant downside of the extrusion approach. Our data incentivize more research to leverage EV membrane biomimicking as a unique drug delivery approach in the near future.

scholarly journals Application of Marine-Based Gold Nanomaterials in Cancer Therapy: A Mini-Review

2021 ◽  
Author(s):  
Neda Baghban ◽  
Arezoo Khoradmehr ◽  
Iraj Nabipour ◽  
Amin Tamadon
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Cancer Therapy ◽  
Drug Delivery System ◽  
Delivery System ◽  
Medical Sciences ◽  
Gold Nanocomposites ◽  
Marine Natural Compound ◽  
Gold Nanomaterials ◽  
Near Future

Cancer is one of the health concerns in modern societies. The application of nanotechnology in medical sciences has created new possibilities for the diagnosis, imaging and the treatment of tumors in humans. The present article reviews the application of marine-based gold nanoparticles in diagnosing and treating cancer. The main data were collected from research article on the application of different marine-based gold nanoparticles in detecting and imaging cancer cells as well as in drug delivery system in treatment of cancer. Chitosan is the most used marine natural compound used to fabricate gold nanocomposites and the most reported application of this type of nano-composites is related to drug delivery system. Despite the excellent anticancer potential of different marine natural products, less studies have been conducted on the use of their compositions with gold nanoparticles in cancer therapy than other materials. Moreover, most reports available in this filed are related to their application as a drug delivery system not anticancer drug. In general, there are still challenges and limitations to the use of nanoparticles in medicine, it is hoped that in the near future nanoparticles will create a dramatic revolution not only in oncology but also in medicine.

Spontaneous ultra fast synthesis of gold nanoparticles using Punica granatum for cancer targeted drug delivery

2013 ◽  
Vol 106 ◽  
pp. 208-216 ◽  
Author(s):  
Moorthy Ganeshkumar ◽  
Muniram Sathishkumar ◽  
Thangavel Ponrasu ◽  
Murugan Girija Dinesh ◽  
Lonchin Suguna
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Targeted Drug Delivery ◽  
Punica Granatum ◽  
Targeted Drug ◽  
Fast Synthesis

scholarly journals Cell-surface remodelling during mammalian erythropoiesis

1982 ◽  
Vol 208 (1) ◽  
pp. 239-242 ◽  
Author(s):  
D C Wraith ◽  
C J Chesterton
Keyword(s):  
Surface Modification ◽  
Membrane Proteins ◽  
Cell Surface ◽  
Protein Composition ◽  
Surface Protein ◽  
Current Evidence ◽  
Cell Surface Protein ◽  
Erythroid Cells ◽  
Selective Loss ◽  
Before And After

Current evidence suggests that the major cell-surface modification occurring during mammalian erythropoiesis could be generated by two separate mechanisms: either selective loss of membrane proteins during enucleation or endocytosis at the subsequent reticulocyte and erythrocyte stages. The former idea was tested by collecting developing rabbit erythroid cells before and after the enucleation step and comparing their cell-surface protein composition via radiolabelling and electrophoresis. Few changes were observed. Our data thus lend support to the endocytosis mechanism.

scholarly journals Structural membrane proteins and loosely associated proteins of the sarcoplasmic reticulum

1974 ◽  
Vol 139 (3) ◽  
pp. 509-513 ◽  
Author(s):  
A. Margreth ◽  
U. Carraro ◽  
G. Salviati
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Membrane Proteins ◽  
Osmotic Shock ◽  
Protein Composition ◽  
Polyacrylamide Gels ◽  
Associated Proteins

The protein composition of sarcoplasmic-reticulum vesicles, either unpurified or after fractionation on sucrose gradients, and with or without previous osmotic shock and sonication, was investigated by electrophoresis in acid polyacrylamide gels. The pattern of release of loosely bound proteins is discussed with respect to their localization in the interior of the vesicles.

scholarly journals Structural characterization of functionalized gold nanoparticles for drug delivery in cancer therapy: a NMR based approach

2015 ◽  
Vol 17 (29) ◽  
pp. 18971-18979 ◽  
Author(s):  
Sílvia C. Coelho ◽  
Maria Rangel ◽  
Maria C. Pereira ◽  
Manuel A. N. Coelho ◽  
Galya Ivanova
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Cancer Therapy ◽  
Structural Characterization ◽  
Au Nanoparticles ◽  
Functionalized Gold Nanoparticles ◽  
Loaded Surface

Structure of Bortezomib loaded, surface functionalized Au nanoparticles.

Sign in / Sign up

Export Citation Format

Share Document