scholarly journals Beneficial Effects of Vitamins K and D3 on Redox Balance of Human Osteoblasts Cultured with Hydroxyapatite-Based Biomaterials

Cells ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 325 ◽  
Author(s):  
Ewa Ambrożewicz ◽  
Marta Muszyńska ◽  
Grażyna Tokajuk ◽  
Grzegorz Grynkiewicz ◽  
Neven Žarković ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Bone Cells ◽  
Antioxidant Properties ◽  
Redox Balance ◽  
The Body ◽  
Human Osteoblasts ◽  
Redox Imbalance ◽  
Beneficial Effects ◽  
Dna Biosynthesis ◽  
Bone Damage

Hydroxyapatite-based biomaterials are commonly used in surgery to repair bone damage. However, the introduction of biomaterials into the body can cause metabolic alterations, including redox imbalance. Because vitamins D3 and K (K1, MK-4, MK-7) have pronounced osteoinductive, anti-inflammatory, and antioxidant properties, it is suggested that they may reduce the adverse effects of biomaterials. The aim of this study was to investigate the effects of vitamins D3 and K, used alone and in combination, on the redox metabolism of human osteoblasts (hFOB 1.19 cell line) cultured in the presence of hydroxyapatite-based biomaterials (Maxgraft, Cerabone, Apatos, and Gen-Os). Culturing of the osteoblasts in the presence of hydroxyapatite-based biomaterials resulted in oxidative stress manifested by increased production of reactive oxygen species and decrease of glutathione level and glutathione peroxidase activity. Such redox imbalance leads to lipid peroxidation manifested by an increase of 4-hydroxynonenal level, which is known to influence the growth of bone cells. Vitamins D3 and K were shown to help maintain redox balance and prevent lipid peroxidation in osteoblasts cultured with hydroxyapatite-based biomaterials. The strongest effect was observed for the combination of vitamin D3 and MK-7. Moreover, vitamins promoted growth of the osteoblasts, manifested by increased DNA biosynthesis. Therefore, it is suggested that the use of vitamins D3 and K may protect redox balance and support the growth of osteoblasts affected by hydroxyapatite-based biomaterials.

scholarly journals The mTORC2 Regulator homer1 Modulates Protein Levels and Sub-Cellular Localization of the CaSR in Human Osteoblasts.

2021 ◽  
Author(s):  
Mark S Rybchyn ◽  
Tara C Brennan-Speranza ◽  
David Mor ◽  
Zhiqiang Cheng ◽  
Wenhan Chang ◽  
...  
Keyword(s):  
Cellular Localization ◽  
Bone Cells ◽  
Cell Protein ◽  
Osteosarcoma Cell ◽  
Wild Type ◽  
Human Osteoblasts ◽  
Beneficial Effects ◽  
Protein Levels ◽  
Mtorc1 Activation

We recently found that in human osteoblasts Homer1 complexes to CaSR and mediates AKT initiation via mTORC2 leading to beneficial effects in osteoblasts including -catenin stabilization and mTORC1 activation (doi: 10.1074/jbc.RA118.006587). Herein we further investigated the relationship between Homer1 and CaSR and demonstrate a link between the protein levels of CaSR and Homer1 in human osteoblasts in primary culture. Thus, when siRNA was used to suppress the CaSR, we observed upregulated Homer1 levels and when siRNA was used to suppress Homer1 we observed downregulated CaSR protein levels using immunofluorescence staining of cultured osteoblasts as well as western blot analyses of cell protein extracts. This finding was confirmed in vivo as the bone cells from osteoblast specific CaSR(-/-) mice showed increased Homer1 expression compared to wild-type. Furthermore, when the commonly used osteosarcoma cell lines MG63 and SAOS-2 were compared to primary osteoblasts, higher levels of Homer1 protein were associated with increased protein levels of the CaSR as well as mTOR and Rictor. CaSR and Homer1 protein were both expressed in osteocytes embedded in the long bones of wild-type mice, and immunofluorescent studies of these cells revealed that Homer1 protein sub-cellular localization was markedly altered in the osteocytes of CaSR(-/-) mice compared to wt. The study identifies additional roles for Homer1 in the control of the protein level and subcellular localization of CaSR in cells of the osteoblast lineage, in addition to its established role of mTORC2 activation downstream of the receptor.

Bone turnover

2013 ◽  
pp. 394-397
Author(s):  
Georg Schett
Keyword(s):  
Vitamin D ◽  
Bone Cells ◽  
Bone Matrix ◽  
The Body ◽  
Matrix Proteins ◽  
Parathyroid Hormones ◽  
Haematopoietic Cells ◽  
Bone Damage ◽  
Bone Matrix Proteins ◽  
The Right

Bone is a dynamic tissue, which undergoes continuous remodelling throughout life. This process requires deposition of new bone, a process which is controlled by the bone-forming osteoblasts, as well as the degradation of bone, exerted by the bone-resorbing osteoclasts. Osteoblast differentiate from mesenchymal precursors and are metabolically active cells, which produce bone matrix proteins such as collagen and osteocalcin, as well as alkaline phosphatase. These proteins can be used to measure osteoblast function in humans. Osteoclasts are haematopoietic cells of the monocyte linage degrading the bone by enzymes cleaving collagen such as metalloproteinases and cathepsins. Collagen cleavage products are indicators for bone resorption in the body. Osteoblast-osteoclast actions are controlled by a fine network of bone cells, osteocytes, which are responsible for sensing bone damage and directing bone remodelling to the right anatomical locations. Finally, mineralization of the newly formed bone is governed by vitamin D and parathyroid hormones, the key regulators of calcium homeostasis in our body.

Antioxidant properties of ferulic acid and the main results of their implementation in the industrial incubation of turkeys

2021 ◽  
Vol 1 (1) ◽  
pp. 76-84
Author(s):  
T. O. Azarnova ◽  
◽  
A. M. Rezvykh ◽  
V. I. Maksimov ◽  
I. I. Kochish ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Ferulic Acid ◽  
Carbohydrate Metabolism ◽  
Old Age ◽  
Antioxidant Properties ◽  
Live Weight ◽  
The Body ◽  
Diene Conjugates ◽  
Turkey Poults ◽  
Experimental Group

The presented article presented data confirming the antioxidant properties of ferulic acid, which is associated with inhibition of the intensity of lipid peroxidation in the body of day-old turkeys, expressed in a decrease in the concentration of its cytotoxic products formed at different stages of the peroxide cascade: isolated double bonds (IDB) by 1,2 times, diene conjugates (DC) 1,6 times (p<0,001,.1,1), triene conjugates (TC) and oxodienic conjugates (ODC) 1,4 times, Schiff bases (SB) 12,02 times compared to the control. This helped to optimize the central metabolic processes, in particular carbohydrate metabolism. The paper analyzes these changes, presents and explains their relationship with the increase in quantitative and qualitative indicators of young turkeys at the early stages of ontogenesis. Thus, the superiority of the experimental group over the control of hatching turkey poults was 0,01% (p<6,66), the hatchability of eggs was 8,23% with a tendency to an increase in live weight of individuals of day-old age by 0,5%, the score according to the «Pasgar» scales and «Optistart» by 0,05 (p<1,0) and 0,001 points (p<XNUMX), respectively.

The effect of the drug "Hepaton" on the reaction of lipid peroxidation

2020 ◽  
Vol 2 ◽  
pp. 112-115
Author(s):  
V.S. Ponamarev ◽  
◽  
O.S. Popova ◽  
Keyword(s):  
Lipid Peroxidation ◽  
Body Weight ◽  
Toxic Hepatitis ◽  
Antioxidant Properties ◽  
The Body ◽  
Intragastric Administration ◽  
Bile Formation ◽  
Laboratory Rats ◽  
Acute Toxic Hepatitis ◽  
Acute Toxic

The composition of the drug "Hepaton" includes many bioactive compounds that provide the antioxidant properties of the drug, manifested by the neutralization of reactive oxygen species (ROS) with the breakaway chain free radical reactions. The aim of the study was to determine the effect of the Hepaton preparation on lipid peroxidation reactions in laboratory rats. Evaluation of the antioxidant effect of the drug was carried out on twenty laboratory rats of both sexes with a body weight of 180-220 g, divided into 2 groups. Acute toxic hepatitis in rats was induced by a single intragastric administration of 1.0 ml of a dichloroethane solution. At the same time, rats of the experimental group (n = 10) 1 hour before the introduction of di-chloroethane were injected with a solution of the drug “Hepaton” in the amount of 10 ml / kg body weight and then 1 time per day for 21 days after the use of toxicants [1]. On the 21st day after the administration of toxicants, a blood was drawn for a biochemi-cal study, which took into account the pa-rameters of the antioxidant system (diene conjugates (DC), ketodienes (CD) and malondialdehyde (MDA), as well as the level of endogenous intoxication (according to the content of MSM). Based on the results obtained, it can be con-cluded that the use of “Hepaton” in modeling acute toxic hepatitis made it possible to re-store the disturbed homeostasis of the labora-tory animal organism, the structure and in-tegrity of the hepatocyte membranes, inhibit lipid peroxidation as one of the links in the pathogenesis of hepatitis, stimulate antioxi-dant defense and power the endogenous anti-oxidant system of the body, bile formation and biliary excretion, as well as activate the reparative processes of the liver tissue at the cellular and intracellular levels.

scholarly journals Studying the processes of lipid peroxidal oxidation in the organism of fresh cows under the antioxidant impact

2020 ◽  
Vol 176 ◽  
pp. 02001
Author(s):  
Lidiya Kashirina ◽  
Konstantin Ivanischev ◽  
Kirill Romanov
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Properties ◽  
The Body ◽  
Control Group ◽  
Lactation Period ◽  
Black And White ◽  
Inhibiting Effect ◽  
Peroxidation Processes ◽  
Quality Of Milk

The article contains information on the state of the processes of lipid peroxidation (LPO) in fresh cows under the influence of stress reaction caused by labor and the beginning of the lactation period. To reduce oxidative stress in the body of fresh cows, antioxidants were used in the form of preparations “E-selenium” and “Butofan”. The aim of the research was to determine the effect of vitamin-containing preparations “E-selenium” and “Butofan” on the productivity of fresh cows, the quality of milk and butter, made from it. The studies were carried out during the winter-stall period on one of the farms of Ryazan district of Ryazan region and involved 12 fresh black-and-white cows aged 4 years. The control group of animals was intact. As a result of the investigations, it was found that due to the action of the above preparations, which have antioxidant properties, the productivity parameters in the experimental groups of animals were higher compared to the control group, because they had an inhibiting effect on lipid peroxidation processes in the organism and activated the work of its own antioxidant system.

scholarly journals The quality of dairy products made from the milk of cows consuming vitamin-containing preparations

2020 ◽  
Vol 17 ◽  
pp. 00096
Author(s):  
Lydia G. Kashirina ◽  
Konstantin A. Ivanishchev ◽  
Kirill I. Romanov
Keyword(s):  
Lipid Peroxidation ◽  
Dairy Products ◽  
Antioxidant Properties ◽  
The Body ◽  
Control Group ◽  
Lactation Period ◽  
Black And White ◽  
Peroxidation Processes ◽  
Quality Of Milk

The article contains information on the state of the processes of lipid peroxidation (LPO) in fresh cows under the influence of stress reaction caused by labor and the beginning of the lactation period. To reduce oxidative stress in the body of fresh cows, antioxidants were used in the form of preparations “E-selenium” and “Butofan”. The aim of the research was to determine the effect of vitamin-containing preparations “E-selenium” and “Butofan” on the productivity of fresh cows, the quality of milk and butter, made from it. The studies were carried out during the winter-stall period on one of the farms of Ryazan district of Ryazan region and involved 12 fresh black-and-white cows aged 4 years. The control group of animals was intact. As a result of the investigations, it was found that due to the action of the above preparations, which have antioxidant properties, the productivity parameters in the experimental groups of animals were higher compared to the control group, because they had an inhibiting effect on lipid peroxidation processes in the organism and activated the work of its own antioxidant system.

scholarly journals Influence of feed additive “Butaselmevit-Plus” on antioxidant status of rats in conditions of oxidative stress

2019 ◽  
Vol 21 (90) ◽  
pp. 76-81 ◽  
Author(s):  
T. V. Martyshuk ◽  
B. V. Gutyj
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Reduced Glutathione ◽  
Antioxidant Properties ◽  
The Body ◽  
Feed Additive ◽  
Antioxidant Protection ◽  
Feed Supplement ◽  
Experimental Group

The purpose of the work was to investigate the effect of the feed supplement “Butaselmevit Plus” on the antioxidant status of the organism in rats under conditions of oxidative stress. The research was carried out on white, sexually-mature, young male rats of the Wistar line, with a body weight of 180–200 g, which was kept on a standard diet of the Institute vivarium of the State Scientific-Research Control Institute of Veterinary Preparations and Feed Supplements. The animals were divided into three groups of 20 animals in each: 1st group (K) intact animals; Group 2 (R1) – rats, affected with tetrachloromethane; Group 3 (R2) – rats, affected with tetrachloromethane and used as a feed additive “Butaselmevit-Plus”. The feed supplement “Butaselmevit-Plus” includes the fruits of thistle spotted, methionine, sodium selenite and vitamins A, E, D3. Significant violation of oxidative-antioxidant balance in animals under oxidative stress conditions, which is characterized, primarily, by activation of processes of radical lipid oxidation with excessive accumulation of both intermediate and final products of lipid peroxidation oxidation and inhibition of antioxidant defense system activity, is established. The development of oxidative stress leads to the inhibition of the activity of the glutathione system of antioxidant protection of the body of rats. This is evidenced by the low activity of glutathione peroxidase and the low level of reduced glutathione in the blood of experimental rats. The feed supplement “Butaselmeib-Plus” contributed to the activation of the system of antioxidant protection of the body of rats for tetrachloromethane poisoning, as evidenced by the increased activity of glutathione peroxidase and the level of reduced glutathione. In addition, in the blood of experimental rats, inhibition of lipid peroxidation and formation of free radicals was observed. It was determined that the level of lipids hydroperoxides on the 20th day of the experiment in blood of rats of experimental group R2 decreased by 35.7%, and the level of TBK-active products – by 21.6% relative to the indices of the first experimental group of rats. Thus, the feed supplement “Butaselmevit-Plus” when fed to rats for the development of oxidative stress inhibited lipid peroxidation, as indicated by the low level of lipids hydroperoxides and TBK-active products in their blood. This may be due to the fact that the feed additive includes two strong antioxidants, such as vitamin E and selenium, which in turn enhance the action of each other. It should also be noted that the antioxidant properties of thistle blisters, which, according to the literature, also possess antioxidant properties. It consists of vitamins B, A, E, K, precursors of vitamin D, carotenoids, macroelements – potassium, calcium, magnesium, ferrum and microelements – Cuprum, Zinc, Manganese, Iodine. The combined action of these biologically important elements exhibits high hepatoprotective and antioxidant activity.

scholarly journals Protective Effects of Fucoxanthin on High Glucose- and 4-Hydroxynonenal (4-HNE)-Induced Injury in Human Retinal Pigment Epithelial Cells

Antioxidants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1176
Author(s):  
Yi-Fen Chiang ◽  
Hsin-Yuan Chen ◽  
Yen-Jui Chang ◽  
Yin-Hwa Shih ◽  
Tzong-Ming Shieh ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Epithelial Cells ◽  
Protective Effect ◽  
High Glucose ◽  
Retinal Pigment ◽  
Antioxidant Properties ◽  
The Body ◽  
Biologically Active ◽  
Diabetic Patients ◽  
Blood Retinal Barrier

The incidence of diabetes mellitus is increasing due to the eating and living habits of modern people. As the disease progresses, the long-term effects of diabetes can cause microvascular disease, causing dysfunction in different parts of the body, which, in turn, leads to different complications, such as diabetic neuropathy, diabetic nephropathy, and diabetic retinopathy (DR). DR is the main cause of vision loss and blindness in diabetic patients. Persistent hyperglycemia may cause damage to the retina, induce the accumulation of inflammatory factors, and destroy the blood–retinal barrier function. Fucoxanthin (Fx) is a marine carotenoid extracted from seaweed. It accounts for more than 10% of the total carotenoids in nature. Fx is mainly found in brown algae and has strong antioxidant properties, due to its unique biologically active structure. This carotenoid also has the effects of reducing lipid peroxidation, reducing DNA damage, and preventing cardiovascular diseases as well as anti-inflammatory and anti-tumor effects. However, there is no relevant research on the protective effect of Fx in DR. Therefore, in this study, we explore the protective effect of Fx on the retina. Human retinal epithelial cells (ARPE-19) are used to investigate the protective effect of Fx on high glucose stress- (glucose 75 mM) and high lipid peroxidation stress (4-hydroxynonenal, 4-HNE (30 μM))-induced DR. The cell viability test shows that Fx recovered the cell damage, and Western blotting shows that Fx reduced the inflammation response and maintained the integrity of the blood–retinal barrier by reducing its apoptosis and cell adhesion factor protein expression. Using an antioxidant enzyme assay kit, we find that the protective effect of Fx may be related to the strong antioxidant properties of Fx, which increases catalase and reduces oxidative stress to produce a protective effect on the retina.

scholarly journals Anti-Osteoclastic Effect of Zinc Studied in MCF-7 Induced Osteoclastogenesis

2019 ◽  
Vol 9 (4-s) ◽  
pp. 94-97
Author(s):  
K Nagalakshmi ◽  
S Shila ◽  
P Rasappan
Keyword(s):  
Oxidative Stress ◽  
Bone Metastasis ◽  
Bone Cells ◽  
Bone Remodelling ◽  
Antioxidant Properties ◽  
Cathepsin K ◽  
Protective Role ◽  
The Body ◽  
Mrna Levels ◽  
Mcf 7

Bone which a key structural support of the body, undergoes dynamic micro structural remodelling all over life to control automatic stress and calcium requirement in the body. A number of risk factors including oxidative stress, apoptosis and abnormal intracellular Ca2+ metabolism have been postulated to play a function in the inception and progress of bone osteolysis. Cancer cells establish a tight relationship with the host tissue, secreting factors that stimulate or inhibit bone cells, receiving signals generated from the bone remodelling activity, and displaying some features of bone cells. This interplay between tumour and bone cells alters the physiological bone remodelling, leading to the generation of a vicious cycle that promotes bone metastasis growth. Zinc is one of the most relevant minerals to human health, because of its antioxidant properties. The present study was aimed to investigate protective role of zinc against bone metastasis. In the present study, TRAP positive multinucleated cell count was low compared to CM treated cells. Zinc treatment suppressed MCF-7 induced mRNA levels of cytoplasmic 1 (Nfatc1), TRAP and Cathepsin-K. Hence, it can be concluded that zinc decreases osteoclastogenesis induced by MCF-7 cells. Keywords: Oxidative stress; Metastasis; Bone remodelling; Zinc.

Effects of intermittent hypoxia different regimes on mitochondrial lipid peroxidation and glutathione-redox balance in stressed rats

2008 ◽  
Vol 3 (3) ◽  
pp. 233-242 ◽  
Author(s):  
Olga Gonchar
Keyword(s):  
Lipid Peroxidation ◽  
Intermittent Hypoxia ◽  
Antioxidant Defense System ◽  
Redox Balance ◽  
Liver Mitochondria ◽  
Sod Activity ◽  
Beneficial Effects ◽  
Late Preconditioning ◽  
Mitochondrial Superoxide

AbstractThe purpose of this study was to compare the influence of two regimes of intermittent hypoxia (IH) [repetitive 5 cycles of 5 min hypoxia (7% O2 or 12% O2 in N2) followed by 15 min normoxia, daily for three weeks] on oxidative stress protective systems in liver mitochondria. To estimate the effectiveness of hypoxia adaptation at the early and late preconditioning period, we exposed rats to acute 6-h immobilization at the 1st and 45th days after cessation of IH. We showed that severity of hypoxic episodes during IH might initiate different adaptive programs. Moderate hypoxia during IH prevents mitochondrial glutathione pool depletion induced by immobilization stress, maintains GSH-redox cycle via activation of glutathione peroxidase, glutathione-S-transferase, glutathione reductase, NADP+-dependent isocitrate dehydrogenase, and increases Mn-SOD activity. Such regimen of hypoxic preconditioning caused the decrease of mitochondrial superoxide anion generation as well as of basal and stimulated in vitro lipid peroxidation and this protective effect remained for 45 days under renormoxic conditions. Hypoxic adaptation in a more severe regimen exerted beneficial effects on the mitochondrial antioxidant defense system only at its later phase.

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