scholarly journals A Review of Single-Cell Adhesion Force Kinetics and Applications

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 577
Author(s):  
Ashwini Shinde ◽  
Kavitha Illath ◽  
Pallavi Gupta ◽  
Pallavi Shinde ◽  
Ki-Taek Lim ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Developmental Disorders ◽  
Quantitative Methods ◽  
Adhesion Force ◽  
Force Measurement ◽  
Adhesive Force ◽  
A Cell ◽  
Sense And Respond ◽  
Cellular Deformability

Cells exert, sense, and respond to the different physical forces through diverse mechanisms and translating them into biochemical signals. The adhesion of cells is crucial in various developmental functions, such as to maintain tissue morphogenesis and homeostasis and activate critical signaling pathways regulating survival, migration, gene expression, and differentiation. More importantly, any mutations of adhesion receptors can lead to developmental disorders and diseases. Thus, it is essential to understand the regulation of cell adhesion during development and its contribution to various conditions with the help of quantitative methods. The techniques involved in offering different functionalities such as surface imaging to detect forces present at the cell-matrix and deliver quantitative parameters will help characterize the changes for various diseases. Here, we have briefly reviewed single-cell mechanical properties for mechanotransduction studies using standard and recently developed techniques. This is used to functionalize from the measurement of cellular deformability to the quantification of the interaction forces generated by a cell and exerted on its surroundings at single-cell with attachment and detachment events. The adhesive force measurement for single-cell microorganisms and single-molecules is emphasized as well. This focused review should be useful in laying out experiments which would bring the method to a broader range of research in the future.

Single cell adhesion force measurement for cell viability identification using an AFM cantilever-based micro putter

2011 ◽  
Vol 22 (11) ◽  
pp. 115802 ◽  
Author(s):  
Yajing Shen ◽  
Masahiro Nakajima ◽  
Seiji Kojima ◽  
Michio Homma ◽  
Masaru Kojima ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Viability ◽  
Single Cell ◽  
Adhesion Force ◽  
Force Measurement ◽  
Afm Cantilever

scholarly journals Single cell micro-pillar-based characterization of endothelial and fibroblast cell mechanics

2021 ◽  
Author(s):  
Julia Eckert ◽  
Yasmine Abouleila ◽  
Thomas Schmidt ◽  
Alireza Mashaghi
Keyword(s):  
Single Cell ◽  
Force Measurement ◽  
Traction Force ◽  
Fibroblast Cell ◽  
Cell Types ◽  
Fibroblast Cells ◽  
Cellular Processes ◽  
Sense And Respond ◽  
Underlying Mechanisms

Mechanotransduction, the ability of cells to sense and respond to the mechanical cues from their microenvironment, plays an important role in numerous cellular processes, ranging from cell migration to differentiation. Several techniques have been developed to investigate the underlying mechanisms of mechanotransduction, in particular, force measurement-based techniques. However, we still lack basic single cell quantitative comparison on the mechanical properties of commonly used cell types, such as endothelial and fibroblast cells. Such information is critical to provide a precedent for studying complex tissues and organs that consist of various cell types. In this short communication, we report on the mechanical characterization of the commonly used endothelial and fibroblast cells at the single cell level. Using a micropillar-based assay, we measured the traction force profiles of these cells. Our study showcases differences between the two cell types in their traction force distribution and morphology. The results reported can be used as a reference and to lay the groundwork for future analysis of numerous disease models involving these cells.

scholarly journals Single-cell adhesion force kinetics of cell populations from combined label-free optical biosensor and robotic fluidic force microscopy

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Milan Sztilkovics ◽  
Tamas Gerecsei ◽  
Beatrix Peter ◽  
Andras Saftics ◽  
Sandor Kurunczi ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Temporal Resolution ◽  
Adhesion Force ◽  
Optical Biosensor ◽  
Cell Populations ◽  
Label Free ◽  
Kinetic Evaluation ◽  
Force Microscopy ◽  
Kinetics Of

AbstractSingle-cell adhesion force plays a crucial role in biological sciences, however its in-depth investigation is hindered by the extremely low throughput and the lack of temporal resolution of present techniques. While atomic force microcopy (AFM) based methods are capable of directly measuring the detachment force values between individual cells and a substrate, their throughput is limited to few cells per day, and cannot provide the kinetic evaluation of the adhesion force over the timescale of several hours. In this study a high spatial and temporal resolution resonant waveguide grating based label-free optical biosensor was combined with robotic fluidic force microscopy to monitor the adhesion of living cancer cells. In contrast to traditional fluidic force microscopy methods with a manipulation range in the order of 300–400 micrometers, the robotic device employed here can address single cells over mm-cm scale areas. This feature significantly increased measurement throughput, and opened the way to combine the technology with the employed microplate-based, large area biosensor. After calibrating the biosensor signals with the direct force measuring technology on 30 individual cells, the kinetic evaluation of the adhesion force and energy of large cell populations was performed for the first time. We concluded that the distribution of the single-cell adhesion force and energy can be fitted by log-normal functions as cells are spreading on the surface and revealed the dynamic changes in these distributions. The present methodology opens the way for the quantitative assessment of the kinetics of single-cell adhesion force and energy with an unprecedented throughput and time resolution, in a completely non-invasive manner.

scholarly journals Decoding Cytoskeleton-Anchored and Non-Anchored Receptors from Single-Cell Adhesion Force Data

2015 ◽  
Vol 109 (7) ◽  
pp. 1330-1333 ◽  
Author(s):  
Ediz Sariisik ◽  
Cvetan Popov ◽  
Jochen P. Müller ◽  
Denitsa Docheva ◽  
Hauke Clausen-Schaumann ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Adhesion Force ◽  
Force Data

scholarly journals Single-cell adhesion strength and contact density drops in the M phase of cancer cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rita Ungai-Salánki ◽  
Eleonóra Haty ◽  
Tamás Gerecsei ◽  
Barbara Francz ◽  
Bálint Béres ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Cancer Cells ◽  
Adhesion Strength ◽  
Adhesion Force ◽  
Single Cell Level ◽  
Cell Level ◽  
Contact Density ◽  
M Phase ◽  
Tissue Cells

AbstractThe high throughput, cost effective and sensitive quantification of cell adhesion strength at the single-cell level is still a challenging task. The adhesion force between tissue cells and their environment is crucial in all multicellular organisms. Integrins transmit force between the intracellular cytoskeleton and the extracellular matrix. This force is not only a mechanical interaction but a way of signal transduction as well. For instance, adhesion-dependent cells switch to an apoptotic mode in the lack of adhesion forces. Adhesion of tumor cells is a potential therapeutic target, as it is actively modulated during tissue invasion and cell release to the bloodstream resulting in metastasis. We investigated the integrin-mediated adhesion between cancer cells and their RGD (Arg-Gly-Asp) motif displaying biomimetic substratum using the HeLa cell line transfected by the Fucci fluorescent cell cycle reporter construct. We employed a computer-controlled micropipette and a high spatial resolution label-free resonant waveguide grating-based optical sensor calibrated to adhesion force and energy at the single-cell level. We found that the overall adhesion strength of single cancer cells is approximately constant in all phases except the mitotic (M) phase with a significantly lower adhesion. Single-cell evanescent field based biosensor measurements revealed that at the mitotic phase the cell material mass per unit area inside the cell-substratum contact zone is significantly less, too. Importantly, the weaker mitotic adhesion is not simply a direct consequence of the measured smaller contact area. Our results highlight these differences in the mitotic reticular adhesions and confirm that cell adhesion is a promising target of selective cancer drugs as the vast majority of normal, differentiated tissue cells do not enter the M phase and do not divide.

Cell-cell adhesion force measurement using nano picker via nanorobotic manipulators inside ESEM

2010 ◽  
Author(s):  
Yajing Shen ◽  
Mohd Ridzuan Ahmad ◽  
Masahiro Nakajima ◽  
Seiji Kojima ◽  
Michio Homma ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Adhesion Force ◽  
Force Measurement ◽  
Cell Cell

scholarly journals Influence of Yeast Extract on the Cytotoxicity of PSL Nanoparticles and Adhesion Force Measurement between a Nanoparticle and a Cell Using AFM

2016 ◽  
Vol 53 (12) ◽  
pp. 762-767 ◽  
Author(s):  
Shohei Yumiyama ◽  
Eri Fujisawa ◽  
Shunsuke Toyoda ◽  
Yuta Kuriyama ◽  
Yasuhiro Konishi ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Adhesion Force ◽  
Force Measurement ◽  
A Cell

scholarly journals Single-Cell Probe Force Studies to Identify Sox2 Overexpression-Promoted Cell Adhesion in MCF7 Breast Cancer Cells

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 935
Author(s):  
Jagoba Iturri ◽  
Andreas Weber ◽  
María d.M. Vivanco ◽  
José L. Toca-Herrera
Keyword(s):  
Breast Cancer ◽  
Cell Adhesion ◽  
Single Cell ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Force Measurement ◽  
Adhesion Forces ◽  
Adhesion Properties ◽  
Area Of Interest

The replacement of the cantilever tip by a living cell in Atomic Force Microscopy (AFM) experiments permits the direct quantification of cell–substrate and cell–cell adhesion forces. This single-cell probe force measurement technique, when complemented by microscopy, allows controlled manipulation of the cell with defined location at the area of interest. In this work, a setup based on two glass half-slides, a non-fouling one with bacterial S-layer protein SbpA from L. sphaericus CMM 2177 and the second with a fibronectin layer, has been employed to measure the adhesion of MCF7 breast cancer cells to fibronectin films (using SbpA as control) and to other cells (symmetric vs. asymmetric systems). The measurements aimed to characterize and compare the adhesion capacities of parental cells and cells overexpressing the embryonic transcription factor Sox2, which have a higher capacity for invasion and are more resistant to endocrine therapy in vivo. Together with the use of fluorescence techniques (epifluorescence, Total Internal Fluorescence Microscopy (TIRF)), the visualization of vinculin and actin distribution in cells in contact with fibronectin surfaces is enabled, facilitating the monitoring and quantification of the formation of adhesion complexes. These findings demonstrate the strength of this combined approach to assess and compare the adhesion properties of cell lines and to illustrate the heterogeneity of adhesive strength found in breast cancer cells.

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