scholarly journals Electrochemical Sensing in 3D Cell Culture Models: New Tools for Developing Better Cancer Diagnostics and Treatments

Cancers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1381
Author(s):  
Micaela Oliveira ◽  
Pedro Conceição ◽  
Krishna Kant ◽  
Alar Ainla ◽  
Lorena Diéguez
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Cancer Diagnostics ◽  
Electrochemical Sensing ◽  
Model Systems ◽  
Sensing Applications ◽  
Electrochemical Biosensing ◽  
3D Microenvironment ◽  
Culture Models ◽  
Cell Culture Models

Currently, conventional pre-clinical in vitro studies are primarily based on two-dimensional (2D) cell culture models, which are usually limited in mimicking the real three-dimensional (3D) physiological conditions, cell heterogeneity, cell to cell interaction, and extracellular matrix (ECM) present in living tissues. Traditionally, animal models are used to mimic the 3D environment of tissues and organs, but they suffer from high costs, are time consuming, bring up ethical concerns, and still present many differences when compared to the human body. The applications of microfluidic-based 3D cell culture models are advantageous and useful as they include 3D multicellular model systems (MCMS). These models have demonstrated potential to simulate the in vivo 3D microenvironment with relatively low cost and high throughput. The incorporation of monitoring capabilities in the MCMS has also been explored to evaluate in real time biophysical and chemical parameters of the system, for example temperature, oxygen, pH, and metabolites. Electrochemical sensing is considered as one of the most sensitive and commercially adapted technologies for bio-sensing applications. Amalgamation of electrochemical biosensing with cell culture in microfluidic devices with improved sensitivity and performance are the future of 3D systems. Particularly in cancer, such models with integrated sensing capabilities can be crucial to assess the multiple parameters involved in tumour formation, proliferation, and invasion. In this review, we are focusing on existing 3D cell culture systems with integrated electrochemical sensing for potential applications in cancer models to advance diagnosis and treatment. We discuss their design, sensing principle, and application in the biomedical area to understand the potential relevance of miniaturized electrochemical hybrid systems for the next generation of diagnostic platforms for precision medicine.

scholarly journals Conjunctival melanoma and electrochemotherapy: preliminary results using 2D and 3D cell culture models in vitro

2018 ◽  
Vol 97 (4) ◽  
pp. e632-e640 ◽  
Author(s):  
Miltiadis Fiorentzis ◽  
Periklis Katopodis ◽  
Helen Kalirai ◽  
Berthold Seitz ◽  
Arne Viestenz ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Conjunctival Melanoma ◽  
Preliminary Results ◽  
Culture Models ◽  
2D And 3D ◽  
Cell Culture Models

Organotypic 3D cell culture models: using the rotating wall vessel to study host–pathogen interactions

2010 ◽  
Vol 8 (11) ◽  
pp. 791-801 ◽  
Author(s):  
Jennifer Barrila ◽  
Andrea L. Radtke ◽  
Aurélie Crabbé ◽  
Shameema F. Sarker ◽  
Melissa M. Herbst-Kralovetz ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Host Pathogen Interactions ◽  
Rotating Wall ◽  
Rotating Wall Vessel ◽  
Host Pathogen ◽  
Culture Models ◽  
Cell Culture Models

Length vs. stiffness: which plays a dominant role in the cellular uptake of fructose-based rod-like micelles by breast cancer cells in 2D and 3D cell culture models?

2018 ◽  
Vol 6 (25) ◽  
pp. 4223-4231 ◽  
Author(s):  
Jiacheng Zhao ◽  
Hongxu Lu ◽  
Yin Yao ◽  
Sylvia Ganda ◽  
Martina H. Stenzel
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Dominant Role ◽  
3D Cell Culture ◽  
Culture Models ◽  
Nano Rods ◽  
2D And 3D ◽  
Cell Culture Models

Internalization of rod-like micelles by breast cancer cells is significantly affected by the stiffness of nano-rods.

scholarly journals Untargeted Metabolite Mapping in 3D Cell Culture Models Using High Spectral Resolution FT-ICR Mass Spectrometry Imaging

2019 ◽  
Vol 91 (15) ◽  
pp. 9522-9529 ◽  
Author(s):  
Lulu H. Tucker ◽  
Gregory R. Hamm ◽  
Rebecca J. E. Sargeant ◽  
Richard J. A. Goodwin ◽  
C. Logan Mackay ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Culture ◽  
Spectral Resolution ◽  
Mass Spectrometry Imaging ◽  
3D Cell Culture ◽  
High Spectral Resolution ◽  
Culture Models ◽  
Cell Culture Models ◽  
Ft Icr

scholarly journals The Communication between Ocular Surface and Nasal Epithelia in 3D Cell Culture Technology for Translational Research: A Narrative Review

2021 ◽  
Vol 22 (23) ◽  
pp. 12994
Author(s):  
Malik Aydin ◽  
Jana Dietrich ◽  
Joana Witt ◽  
Maximiliane S. C. Finkbeiner ◽  
Jonas J.-H. Park ◽  
...  
Keyword(s):  
Cell Culture ◽  
Nasal Cavity ◽  
Ocular Surface ◽  
Measles Virus ◽  
3D Culture ◽  
3D Cell Culture ◽  
Narrative Review ◽  
Culture Models ◽  
Cell Culture Models

There is a lack of knowledge regarding the connection between the ocular and nasal epithelia. This narrative review focuses on conjunctival, corneal, ultrastructural corneal stroma, and nasal epithelia as well as an introduction into their interconnections. We describe in detail the morphology and physiology of the ocular surface, the nasolacrimal ducts, and the nasal cavity. This knowledge provides a basis for functional studies and the development of relevant cell culture models that can be used to investigate the pathogenesis of diseases related to these complex structures. Moreover, we also provide a state-of-the-art overview regarding the development of 3D culture models, which allow for addressing research questions in models resembling the in vivo situation. In particular, we give an overview of the current developments of corneal 3D and organoid models, as well as 3D cell culture models of epithelia with goblet cells (conjunctiva and nasal cavity). The benefits and shortcomings of these cell culture models are discussed. As examples for pathogens related to ocular and nasal epithelia, we discuss infections caused by adenovirus and measles virus. In addition to pathogens, also external triggers such as allergens can cause rhinoconjunctivitis. These diseases exemplify the interconnections between the ocular surface and nasal epithelia in a molecular and clinical context. With a final translational section on optical coherence tomography (OCT), we provide an overview about the applicability of this technique in basic research and clinical ophthalmology. The techniques presented herein will be instrumental in further elucidating the functional interrelations and crosstalk between ocular and nasal epithelia.

Imaging and profiling lung fibroblasts in 3D cell culture models

Pneumologie ◽  
2011 ◽  
Vol 65 (12) ◽  
Author(s):  
G Burgstaller ◽  
M Kronberger ◽  
B Oehrle ◽  
O Eickelberg
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Lung Fibroblasts ◽  
Culture Models ◽  
Cell Culture Models
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