scholarly journals Surface Functionalization by Hydrophobin-EPSPS Fusion Protein Allows for the Fast and Simple Detection of Glyphosate

Biosensors ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 104 ◽  
Author(s):  
Julia Döring ◽  
David Rettke ◽  
Gerhard Rödel ◽  
Tilo Pompe ◽  
Kai Ostermann
Keyword(s):  
Surface Functionalization ◽  
Inorganic Phosphate ◽  
Surface Immobilization ◽  
Simple Method ◽  
New Approach ◽  
Simple Detection ◽  
Photometric Technique ◽  
Malachite Green Assay ◽  
Nanomolar Range

Glyphosate, the most widely used pesticide worldwide, is under debate due to its potentially cancerogenic effects and harmful influence on biodiversity and environment. Therefore, the detection of glyphosate in water, food or environmental probes is of high interest. Currently detection of glyphosate usually requires specialized, costly instruments, is labor intensive and time consuming. Here we present a fast and simple method to detect glyphosate in the nanomolar range based on the surface immobilization of glyphosate’s target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) via fusion to the hydrophobin Ccg2 and determination of enzyme activity with a malachite green assay, which is a common photometric technique to measure inorganic phosphate (Pi). The assay demonstrates a new approach for a fast and simple detection of pesticides.

Development of a simple method for the determination of single nucleotide polymorphisms

2010 ◽  
Vol 34 (8) ◽  
pp. S75-S75
Author(s):  
Weifeng Zhu ◽  
Zhuoqi Liu ◽  
Daya Luo ◽  
Xinyao Wu ◽  
Fusheng Wan
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Nucleotide Polymorphisms ◽  
Simple Method ◽  
Single Nucleotide

The Dudley Lee Case: A new approach to factual causation and its implications for transformative jurisprudence

2017 ◽  
Vol 30 (1) ◽  
pp. 273-289
Author(s):  
Anmari Meerkotter
Keyword(s):  
Case Note ◽  
Constitutional Court ◽  
Recent Contribution ◽  
New Approach ◽  
State Accountability ◽  
The Law ◽  
Transformative Impact ◽  
Constitutional Jurisprudence ◽  
Law Of Delict

The Constitutional Court (CC) judgment of Lee v Minister of Correction Services 2013 2SA 144 (CC) is a recent contribution to transformative constitutional jurisprudence in the field of the law of delict. This matter turned on the issue of factual causation in the context of wrongful and negligent systemic omissions by the state. In this case note, I explore the law relating to this element of delictual liability with specific regard to the traditional test for factual causation – the conditio sine qua non (‘but-for’) test. In particular, I note the problems occasioned by formalistic adherence to this test in the context of systemic state omissions as evidenced by the SCA judgment in the same matter. I also consider the manner in which English courts have addressed this problem. Thereafter, I analyse the CC’s broader approach to the determination of factual causation as one based on common sense and justice. I argue that this approach endorses a break from a formalistic application of the test and constitutes a step towards an approach which resonates with the foundational constitutional values of freedom, dignity and equality. Furthermore, it presents an appropriate solution to the problems associated with factual causation where systemic omissions are concerned. I then consider the transformative impact of the Lee judgment. In particular, I argue that the broader enquiry favoured by the CC facilitates the realisation of constitutionally guaranteed state accountability, and amounts to an extension of the existing norm of accountability jurisprudence. Hence, I contend that the judgment presents a further effort by the Constitutional Court to effect wholesale the constitutionalisation of the law of delict, as well as a vindicatory tool to be used by litigants who have been adversely affected by systemic state omissions.

Automatic Determination of Optimal FIB Operations for Improved Circuit Probing and Fast Reconfiguration

2000 ◽  
Author(s):  
Romain Desplats ◽  
Timothee Dargnies ◽  
Jean-Christophe Courrege ◽  
Philippe Perdu ◽  
Jean-Louis Noullet
Keyword(s):  
Integrated Circuit ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Semiconductor Devices ◽  
Front Side ◽  
Automatic Determination ◽  
Metal Line ◽  
New Approach ◽  
Metal Layers

Abstract Focused Ion Beam (FIB) tools are widely used for Integrated Circuit (IC) debug and repair. With the increasing density of recent semiconductor devices, FIB operations are increasingly challenged, requiring access through 4 or more metal layers to reach a metal line of interest. In some cases, accessibility from the front side, through these metal layers, is so limited that backside FIB operations appear to be the most appropriate approach. The questions to be resolved before starting frontside or backside FIB operations on a device are: 1. Is it do-able, are the metal lines accessible? 2. What is the optimal positioning (e.g. accessing a metal 2 line is much faster and easier than digging down to a metal 6 line)? (for the backside) 3. What risk, time and cost are involved in FIB operations? In this paper, we will present a new approach, which allows the FIB user or designer to calculate the optimal FIB operation for debug and IC repair. It automatically selects the fastest and easiest milling and deposition FIB operations.

A Standard Primary Toxicity Bioassay Using Daphnia Magna as a Test Species

1977 ◽  
Vol 12 (1) ◽  
pp. 27-50 ◽  
Author(s):  
L.A. Behie ◽  
J.E. Zajic ◽  
D. Berk ◽  
R.J.P. Brouzes ◽  
V.A. Naish
Keyword(s):  
Statistical Analysis ◽  
Daphnia Magna ◽  
Biological Indicator ◽  
Pulp And Paper ◽  
Toxicity Bioassay ◽  
Simple Method ◽  
Test Species ◽  
Test Organisms ◽  
Dilution Water

Abstract Although Daphnia magna have been widely used in the determination of the toxicity of various substances, there are no reports in the literature that describe a rigorous bioassay method using this organism as a test species. The test described herein involves the standariza-tion of various important aspects of the method such as the age of the test organisms, and the dilution water used for the preparation of the various toxicant concentrations. Also described is a simple method for the statistical analysis of the results. The sensitivity of the proposed bioassay is demonstrated by determining the toxicity of various pulp and paper effluents. Finally, extensive bioassays were carried out simultaneously with rainbow trout and Daphnia magna indicating that Daphnia are as good a biological indicator of acute toxicity as fish.

scholarly journals New Method for Analysis of the Temporomandibular Joint Using Cone Beam Computed Tomography

Sensors ◽  
2021 ◽  
Vol 21 (9) ◽  
pp. 3070
Author(s):  
Sebastian Iwaszenko ◽  
Jakub Munk ◽  
Stefan Baron ◽  
Adam Smoliński
Keyword(s):  
Computed Tomography ◽  
Temporomandibular Joint ◽  
Cross Section ◽  
Cone Beam Computed Tomography ◽  
Cone Beam ◽  
Section Plane ◽  
New Approach ◽  
Volume Of Interest ◽  
Gap Width

Modern dentistry commonly uses a variety of imaging methods to support diagnosis and treatment. Among them, cone beam computed tomography (CBCT) is particularly useful in presenting head structures, such as the temporomandibular joint (TMJ). The determination of the morphology of the joint is an important part of the diagnosis as well as the monitoring of the treatment results. It can be accomplished by measurement of the TMJ gap width at three selected places, taken at a specific cross-section. This study presents a new approach to these measurements. First, the CBCT images are denoised using curvilinear methods, and the volume of interest is determined. Then, the orientation of the vertical cross-section plane is computed based on segmented axial sections of the TMJ head. Finally, the cross-section plane is used to determine the standardized locations, at which the width of the gap between condyle and fossa is measured. The elaborated method was tested on selected TMJ CBCT scans with satisfactory results. The proposed solution lays the basis for the development of an autonomous method of TMJ index identification.

Hollow fiber supported liquid-phase microextraction combined with maltodextrin-modified capillary electrophoresis for the determination of citalopram enantiomers in urine samples

2015 ◽  
Vol 7 (5) ◽  
pp. 2012-2019 ◽  
Author(s):  
Jafar Abolhasani ◽  
Hamid Reza Jafariyan ◽  
Mohammad Mahdi khataei ◽  
Rahim Hosseinzadeh-khanmiri ◽  
Ebrahim Ghorbani-kalhor ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Liquid Phase ◽  
Hollow Fiber ◽  
Urine Samples ◽  
Simple Method ◽  
Liquid Phase Microextraction ◽  
Supported Liquid Phase

A simple method was developed for the separation and determination of citalopram enantiomers in urine samples.

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