scholarly journals Comparison of Surface Plasmon Resonance, Resonant Waveguide Grating Biosensing and Enzyme Linked Immunosorbent Assay (ELISA) in the Evaluation of a Dengue Virus Immunoassay

Biosensors ◽  
2013 ◽  
Vol 3 (3) ◽  
pp. 297-311 ◽  
Author(s):  
Dongmei Hu ◽  
Scott Fry ◽  
Johnny Huang ◽  
Xixia Ding ◽  
Liwen Qiu ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Dengue Virus ◽  
Surface Plasmon ◽  
Immunosorbent Assay ◽  
Plasmon Resonance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Waveguide Grating ◽  
Resonant Waveguide Grating

scholarly journals Comparison of Surface Plasmon Resonance Imaging and Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies against Iridovirus in Rock Bream (Oplegnathus Fasciatus)

2007 ◽  
Vol 19 (4) ◽  
pp. 414-416 ◽  
Author(s):  
Ho Seong Cho ◽  
Tae Jung Kim
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Immunosorbent Assay ◽  
Plasmon Resonance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Strong Positive Correlation ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Oplegnathus Fasciatus ◽  
Rock Bream

A protein chip based on surface plasmon resonance imaging (SPRI) was developed for detecting fish iridovirus antibody using a recombinant 50-kDa fragment of major capsid protein (MCP) as an antigen. The diagnostic potential of SPRI for measuring antibodies to the iridovirus MCP was compared with that of a conventional enzyme-linked immunosorbent assay (ELISA) using 40 juvenile rock bream ( Oplegnathus fasciatus) serum samples in a nursery. There was a strong positive correlation between the SPRI and ELISA ( n = 40, r = 0.939, P < 0.01). Therefore, this recombinant 50-kDa MCP can be used as an antigen for serological studies, and the SPRI, which is a label-free and high-throughput method, is potentially a valuable tool in the serodiagnosis of an iridoviral infection.

scholarly journals Noble Metal-Assisted Surface Plasmon Resonance Immunosensors

Sensors ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 1003 ◽  
Author(s):  
Jin-Ha Choi ◽  
Jin-Ho Lee ◽  
Joohyung Son ◽  
Jeong-Woo Choi
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Noble Metals ◽  
Specific Antigen ◽  
High Sensitivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Biomarkers ◽  
Detection Probe ◽  
Inducing Factors

For the early diagnosis of several diseases, various biomarkers have been discovered and utilized through the measurement of concentrations in body fluids such as blood, urine, and saliva. The most representative analytical method for biomarker detection is an immunosensor, which exploits the specific antigen-antibody immunoreaction. Among diverse analytical methods, surface plasmon resonance (SPR)-based immunosensors are emerging as a potential detection platform due to high sensitivity, selectivity, and intuitive features. Particularly, SPR-based immunosensors could detect biomarkers without labeling of a specific detection probe, as typical immunosensors such as enzyme-linked immunosorbent assay (ELISA) use enzymes like horseradish peroxidase (HRP). In this review, SPR-based immunosensors utilizing noble metals such as Au and Ag as SPR-inducing factors for the measurement of different types of protein biomarkers, including viruses, microbes, and extracellular vesicles (EV), are briefly introduced.

scholarly journals Quantitative and Selective Surface Plasmon Resonance Response Based on a Reduced Graphene Oxide–Polyamidoamine Nanocomposite for Detection of Dengue Virus E-Proteins

Nanomaterials ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 569 ◽  
Author(s):  
Nur Alia Sheh Omar ◽  
Yap Wing Fen ◽  
Jaafar Abdullah ◽  
Amir Reza Sadrolhosseini ◽  
Yasmin Mustapha Kamil ◽  
...  
Keyword(s):  
Thin Film ◽  
Graphene Oxide ◽  
Surface Plasmon Resonance ◽  
Dengue Virus ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
E Proteins ◽  
Spr Sensor ◽  
Amine Functionalized ◽  
Reduced Graphene

Dengue viral infection is one of the most common deadliest diseases and has become a recurrent issue for public health in tropical countries. Although the spectrum of clinical diagnosis and treatment have recently been established, the efficient and rapid detection of dengue virus (DENV) during viremia and the early febrile phase is still a great challenge. In this study, a dithiobis (succinimidyl undecanoate, DSU)/amine-functionalized reduced graphene oxide-–polyamidoamine dendrimer (DSU/amine-functionalized rGO–PAMAM) thin film-based surface plasmon resonance (SPR) sensor was developed for the detection of DENV 2 E-proteins. Different concentrations of DENV 2 E-proteins were successfully tested by the developed SPR sensor-based system. The performance of the developed sensor showed increased shift in the SPR angle, narrow full-width–half-maximum of the SPR curve, high detection accuracy, excellent figure of merit and signal-to-noise ratio, good sensitivity values in the range of 0.08–0.5 pM (S = 0.2576°/pM, R2 = 0.92), and a high equilibrium association constant (KA) of 7.6452 TM−1. The developed sensor also showed a sensitive and selective response towards DENV 2 E-proteins compared to DENV 1 E-proteins and ZIKV (Zika virus) E-proteins. Overall, it was concluded that the Au/DSU/amine-functionalized rGO–PAMAM thin film-based SPR sensor has potential to serve as a rapid clinical diagnostic tool for DENV infection.

scholarly journals Development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus E-protein

2018 ◽  
Vol 20 ◽  
pp. 16-21 ◽  
Author(s):  
Nur Alia Sheh Omar ◽  
Yap Wing Fen ◽  
Jaafar Abdullah ◽  
Che Engku Noramalina Che Engku Chik ◽  
Mohd Adzir Mahdi
Keyword(s):  
Surface Plasmon Resonance ◽  
Dengue Virus ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Optical Sensor ◽  
Resonance Phenomenon ◽  
E Protein

scholarly journals The detection and identification of dengue virus serotypes with quantum dot and AuNP regulated localized surface plasmon resonance

2020 ◽  
Vol 2 (2) ◽  
pp. 699-709 ◽  
Author(s):  
Ankan Dutta Chowdhury ◽  
Kenshin Takemura ◽  
Indra Memdi Khorish ◽  
Fahmida Nasrin ◽  
Mya Myat Ngwe Tun ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Surface Plasmon Resonance ◽  
Dengue Virus ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Localized Surface Plasmon ◽  
Sensing System ◽  
Detection And Identification ◽  
Dengue Virus Serotypes

A fluorometric sensing system using LSPR phenomenon between fluorescent quantum dots and its adjacent AuNP to detect four closely related but serologically distinct dengue viruses (DENVs).

scholarly journals Surface plasmon resonance analysis for detecting non-structural protein 1 of dengue virus in Indonesia

2020 ◽  
Vol 27 (8) ◽  
pp. 1931-1937
Author(s):  
Widoretno ◽  
Agus Sjahrurachman ◽  
Beti Ernawati Dewi ◽  
Kenny Lischer ◽  
Diah Kartika Pratami ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Dengue Virus ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Structural Protein ◽  
Surface Plasmon Resonance Analysis ◽  
Resonance Analysis
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