Analysis of the Fungicide Boscalid in Horticultural Crops Using an Enzyme-Linked Immunosorbent Assay and an Immunosensor Based on Surface Plasmon Resonance

2015 ◽  
Vol 63 (36) ◽  
pp. 8075-8082 ◽  
Author(s):  
Yuki Hirakawa ◽  
Tomomi Yamasaki ◽  
Ayako Harada ◽  
Toshiya Ohtake ◽  
Kayo Adachi ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Immunosorbent Assay ◽  
Plasmon Resonance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Horticultural Crops

scholarly journals Comparison of Surface Plasmon Resonance Imaging and Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies against Iridovirus in Rock Bream (Oplegnathus Fasciatus)

2007 ◽  
Vol 19 (4) ◽  
pp. 414-416 ◽  
Author(s):  
Ho Seong Cho ◽  
Tae Jung Kim
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Immunosorbent Assay ◽  
Plasmon Resonance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Strong Positive Correlation ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Oplegnathus Fasciatus ◽  
Rock Bream

A protein chip based on surface plasmon resonance imaging (SPRI) was developed for detecting fish iridovirus antibody using a recombinant 50-kDa fragment of major capsid protein (MCP) as an antigen. The diagnostic potential of SPRI for measuring antibodies to the iridovirus MCP was compared with that of a conventional enzyme-linked immunosorbent assay (ELISA) using 40 juvenile rock bream ( Oplegnathus fasciatus) serum samples in a nursery. There was a strong positive correlation between the SPRI and ELISA ( n = 40, r = 0.939, P < 0.01). Therefore, this recombinant 50-kDa MCP can be used as an antigen for serological studies, and the SPRI, which is a label-free and high-throughput method, is potentially a valuable tool in the serodiagnosis of an iridoviral infection.

scholarly journals Noble Metal-Assisted Surface Plasmon Resonance Immunosensors

Sensors ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 1003 ◽  
Author(s):  
Jin-Ha Choi ◽  
Jin-Ho Lee ◽  
Joohyung Son ◽  
Jeong-Woo Choi
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Noble Metals ◽  
Specific Antigen ◽  
High Sensitivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Biomarkers ◽  
Detection Probe ◽  
Inducing Factors

For the early diagnosis of several diseases, various biomarkers have been discovered and utilized through the measurement of concentrations in body fluids such as blood, urine, and saliva. The most representative analytical method for biomarker detection is an immunosensor, which exploits the specific antigen-antibody immunoreaction. Among diverse analytical methods, surface plasmon resonance (SPR)-based immunosensors are emerging as a potential detection platform due to high sensitivity, selectivity, and intuitive features. Particularly, SPR-based immunosensors could detect biomarkers without labeling of a specific detection probe, as typical immunosensors such as enzyme-linked immunosorbent assay (ELISA) use enzymes like horseradish peroxidase (HRP). In this review, SPR-based immunosensors utilizing noble metals such as Au and Ag as SPR-inducing factors for the measurement of different types of protein biomarkers, including viruses, microbes, and extracellular vesicles (EV), are briefly introduced.

scholarly journals Surface Plasmon Resonance Analysis of Antipolysaccharide Antibody Specificity: Responses to Meningococcal Group C Conjugate Vaccines and Bacteria

2004 ◽  
Vol 72 (6) ◽  
pp. 3451-3460 ◽  
Author(s):  
Pablo A. García-Ojeda ◽  
Sharon Hardy ◽  
Steven Kozlowski ◽  
Kathryn E. Stein ◽  
Ian M. Feavers
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Diphtheria Toxin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antibody Specificity ◽  
Surface Plasmon Resonance Analysis ◽  
Conjugate Vaccines ◽  
Resonance Analysis ◽  
Meningococcal Group

ABSTRACT Antibody (Ab) responses to polysaccharides (PS), such as Neisseria meningitidis group C PS (MCPS), are characterized as being thymus independent and are restricted with regard to clonotype and isotype expression. PS conjugated to proteins, e.g., MCPS coupled with tetanus toxoid or the diphtheria toxin derivative CRM197, elicit thymus-dependent responses. The present study developed a surface plasmon resonance approach to evaluate Ab responses to MCPS conjugate vaccines, including either O-acetylated (OAc+) or de-O-acetylated (OAc−) forms of the PS. The results were generally consistent with those obtained by enzyme-linked immunosorbent assay and showed that sera from mice immunized with conjugate vaccines contain Abs that bind more effectively to OAc+ and OAc− MCPS than sera from mice immunized with fixed bacteria. The data suggest a critical shared or overlapping epitope recognized by all the conjugate vaccine immune sera and strategies for assessing polyclonal Ab avidity.

scholarly journals Surface Plasmon Resonance-Based Immunosensor for Igm detection with Gold Nanoparticles

Micromachines ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1092
Author(s):  
Nilay Bereli ◽  
Monireh Bakhshpour ◽  
Aykut Arif Topcu ◽  
Adil Denizli
Keyword(s):  
Aqueous Solution ◽  
Gold Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Limit Of Detection ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Artificial Plasma ◽  
Adsorption Desorption

In this work, a surface plasmon resonance (SPR) based immunosensor was prepared by the immobilization of the amine-functionalized gold nanoparticles (N-AuNPs) on the sensing surface to sense immunoglobulin M (IgM) antibodies in the aqueous solution and artificial plasma. The characterization studies of SPR based immunosensor for IgM detection were performed with scanning electron microscope (SEM), contact angle measurements, and ellipsometry. Kinetic studies for the IgM immunosensor were carried out in the range of 1.0 to 200 ng/mL IgM concentrations in an aqueous solution. The total IgM analysis time including adsorption, desorption, and regeneration cycles was nearly 10 min for the prepared immunosensor. The limit of detection (LOD) and limit of quantification (LOQ) were found as 0.08 and 0.26 ng/mL, respectively. The reusability of the proposed immunosensor was tested with 6 consecutive adsorption-desorption, and regeneration cycles. Also, enzyme-linked immunosorbent assay (ELISA) method was utilized in the validation of the immunosensor.

scholarly journals A surface plasmon resonance based approach for measuring response to pneumococcal vaccine

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marta Garrido-Jareño ◽  
Leonor Puchades-Carrasco ◽  
Leticia Orti-Pérez ◽  
José Miguel Sahuquillo-Arce ◽  
María del Carmen Meyer-García ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Pneumococcal Vaccine ◽  
Pneumococcal Disease ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Response Profiles ◽  
Analytical Approaches

AbstractIncidence of pneumococcal disease has increased worldwide in recent years. Response to pneumococcal vaccine is usually measured using the multiserotype enzyme-linked immunosorbent assay (ELISA) pneumococcal test. However, this approach presents several limitations. Therefore, the introduction of new and more robust analytical approaches able to provide information on the efficacy of the pneumococcal vaccine would be very beneficial for the clinical management of patients. Surface plasmon resonance (SPR) has been shown to offer a valuable understanding of vaccines’ properties over the last years. The aim of this study is to evaluate the reliability of SPR for the anti-pneumococcal capsular polysaccharides (anti-PnPs) IgGs quantification in vaccinated. Fast protein liquid chromatography (FPLC) was used for the isolation of total IgGs from serum samples of vaccinated patients. Binding-SPR assays were performed to study the interaction between anti-PnPs IgGs and PCV13. A robust correlation was found between serum levels of anti-PnPs IgGs, measured by ELISA, and the SPR signal. Moreover, it was possible to correctly classify patients into “non-responder”, “responder” and “high-responder” groups according to their specific SPR PCV13 response profiles. SPR technology provides a valuable tool for reliably characterize the interaction between anti-PnPs IgGs and PCV13 in a very short experimental time.

Comparison of sensitivity of the refractometric methods of frustrated total internal reflection and surface plasmon resonance

2020 ◽  
pp. 44-49
Author(s):  
I. N. Pavlov
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Total Internal Reflection ◽  
Resonance Method ◽  
Internal Reflection ◽  
Optical Methods ◽  
Thin Boundary Layer ◽  
Experimental Conditions ◽  
Frustrated Total Internal Reflection

Two optical methods, namely surface plasmon resonance imaging and frustrated total internal reflection, are described in the paper in terms of comparing their sensitivity to change of refractive index of a thin boundary layer of an investigated medium. It is shown that, despite the fact that the theoretically calculated sensitivity is higher for the frustrated total internal reflection method, and the fact that usually in practice the surface plasmon resonance method, on the contrary, is considered more sensitive, under the same experimental conditions both methods show a similar result.

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