scholarly journals Electrochemical Fingerprint Biosensor for Natural Indigo Dye Yielding Plants Analysis

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 155
Author(s):  
Boyuan Fan ◽  
Qiong Wang ◽  
Weihong Wu ◽  
Qinwei Zhou ◽  
Dongling Li ◽  
...  
Keyword(s):  
Active Components ◽  
Plant Identification ◽  
Health Concerns ◽  
Electrochemically Active ◽  
Gene Level ◽  
Ancient Civilizations ◽  
Indigo Dye ◽  
Electrochemical Fingerprint

Indigo is a plant dye that has been used as an important dye by various ancient civilizations throughout history. Today, due to environmental and health concerns, plant indigo is re-entering the market. Strobilanthes cusia (Nees) Kuntze is the most widely used species in China for indigo preparation. However, other species under Strobilanthes have a similar feature. In this work, 12 Strobilanthes spp. were analyzed using electrochemical fingerprinting technology. Depending on their electrochemically active molecules, they can be quickly identified by fingerprinting. In addition, the fingerprint obtained under different conditions can be used to produce scattered patter and heatmap. These patterns make plant identification more convenient. Since the electrochemically active components in plants reflect the differences at the gene level to some extent, the obtained electrochemical fingerprints are further used for the discussion of phylogenetics.

scholarly journals The Active Ingredients Identification and Antidiarrheal Mechanism Analysis of Plantago asiatica L. Superfine Powder

2021 ◽  
Vol 11 ◽  
Author(s):  
Chun-Liu Dong ◽  
Yue Qin ◽  
Jin-Xin Ma ◽  
Wen-Qiang Cui ◽  
Xing-Ru Chen ◽  
...  
Keyword(s):  
Angle Of Repose ◽  
Mechanism Analysis ◽  
Active Ingredients ◽  
Active Components ◽  
Effective Components ◽  
Tap Density ◽  
Plantago Asiatica ◽  
Gene Level ◽  
Quality Markers ◽  
Antidiarrheal Activity

Plantago asiatica L. is a natural medicinal plant that has been widely used for its various pharmacological effects such as antidiarrheal, anti-inflammatory, and wound healing. This study aims to explore the antidiarrheal active ingredients of Plantago asiatica L. that can be used as quality markers to evaluate P. asiatica L. superfine powder (PSP). Molecular docking experiment was performed to identify the effective components of P. asiatica L., which were further evaluated by an established mouse diarrhea model. Na+/K+-ATPase and creatine kinase (CK) activities and the Na+/K+ concentrations were determined. The gene expression of ckb and Atp1b3 was detected. PSP was prepared and evaluated in terms of the tap density and the angle of repose. The structures of PSPs of different sizes were measured by infrared spectra. The active ingredient contents of PSPs were determined by HPLC. The results indicated that the main antidiarrheal components of P. asiatica L. were luteolin and scutellarein that could increase the concentration of Na+ and K+ by upregulating the activity and gene level of CK and Na+/K+-ATPase. In addition, luteolin and scutellarein could also decrease the volume and weight of small intestinal contents to exert antidiarrheal activity. Moreover, as the PSP size decreased from 6.66 to 3.55 μm, the powder tended to be amorphous and homogenized and of good fluidity, the content of active compounds gradually increased, and the main structure of the molecule remained steady. The optimum particle size of PSP with the highest content of active components was 3.55 μm, and the lowest effective dose for antidiarrhea was 2,000 mg/kg. Therefore, the antidiarrheal active ingredients of PSP were identified as luteolin and scutellarein that exert antidiarrheal activity by binding with Na+/K+-ATPase. PSP was successfully prepared and could be used as a new dosage form for the diarrhea treatment.

Properties of Electrochemically Active Components in Mammalian Vitreous Humor

1997 ◽  
Vol 64 (5) ◽  
pp. 807-812 ◽  
Author(s):  
RICHARD C. ROSE ◽  
RAJESH GOGIA ◽  
STUART P. RICHER
Keyword(s):  
Vitreous Humor ◽  
Active Components ◽  
Electrochemically Active

scholarly journals Biometric Identification of Taxodium spp. and Their Hybrid Progenies by Electrochemical Fingerprints

Biosensors ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 403
Author(s):  
Yuhong Zheng ◽  
Da Wang ◽  
Xiaolong Li ◽  
Ziyang Wang ◽  
Qingwei Zhou ◽  
...  
Keyword(s):  
Pattern Recognition ◽  
Phylogenetic Study ◽  
Biometric Identification ◽  
Hybrid Progeny ◽  
Plant Identification ◽  
Electrochemically Active ◽  
Leaf Tissues ◽  
Plant Hybrid ◽  
The Relationship ◽  
Active Substances

The use of electrochemical fingerprints for plant identification is an emerging application in biosensors. In this work, Taxodium ascendens, T. distichum, T. mucronatum, and 18 of their hybrid progenies were collected for this purpose. This is the first attempt to use electrochemical fingerprinting for the identification of plant hybrid progeny. Electrochemical fingerprinting in the leaves of Taxodium spp. was recorded under two conditions. The results showed that the electrochemical fingerprints of each species and progeny possessed very suitable reproducibility. These electrochemical fingerprints represent the electrochemical behavior of electrochemically active substances in leaf tissues under specific conditions. Since these species and progenies are very closely related to each other, it is challenging to identify them directly using a particular electrochemical fingerprinting. Therefore, electrochemical fingerprints measured under different conditions were used to perform pattern recognition. We can identify different species and progenies by locating the features in different pattern maps. We also performed a phylogenetic study with data from electrochemical fingerprinting. The results proved that the electrochemical classification results and the relationship between them are closely related.

Sexual dysfunction and sexual health concerns in women with diabetes

1998 ◽  
Vol 1 (2) ◽  
pp. 83-87 ◽  
Author(s):  
Meeking ◽  
Fosbury ◽  
Cummings ◽  
Alexander ◽  
Shaw ◽  
...  
Keyword(s):  
Sexual Dysfunction ◽  
Sexual Health ◽  
Health Concerns
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