scholarly journals Ultrasensitive Detection of 2,4-Dinitrophenol Using Nanowire Biosensor

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Yuri D. Ivanov ◽  
Kristina A. Malsagova ◽  
Tatyana O. Pleshakova ◽  
Rafael A. Galiullin ◽  
Andrey F. Kozlov ◽  
...  
Keyword(s):  
Field Effect Transistors ◽  
Silicon Nanowire ◽  
Silicon On Insulator ◽  
Amino Silane ◽  
Protective Layers ◽  
Highly Sensitive ◽  
Wide Range ◽  
Detectable Concentration ◽  
Surface Modification Techniques ◽  
Highly Sensitive Detection

The method for the detection of 2,4-dinitrophenol (DNP) in solution is proposed. This method employs the sensors based on silicon nanowire field-effect transistors with protective layers of high-k dielectrics, whose surface is functionalized with an amino silane. Direct highly sensitive detection of DNP has been demonstrated, and the lowest detectable concentration of DNP was determined to be 10−14 M. Silicon-on-insulator nanowire (SOI-NW) sensors can well be employed for the rapid detection of a wide range of toxic and explosive compounds by selection of sensor surface modification techniques.

scholarly journals Highly Sensitive and Selective Sodium Ion Sensor Based on Silicon Nanowire Dual Gate Field-Effect Transistor

Sensors ◽  
2021 ◽  
Vol 21 (12) ◽  
pp. 4213
Author(s):  
Seong-Kun Cho ◽  
Won-Ju Cho
Keyword(s):  
Field Effect ◽  
Field Effect Transistor ◽  
Silicon Nanowire ◽  
Silicon On Insulator ◽  
Sodium Ion ◽  
Ion Sensor ◽  
Highly Sensitive ◽  
Selective Membrane ◽  
Dual Gate ◽  
Effect Transistor

In this study, a highly sensitive and selective sodium ion sensor consisting of a dual-gate (DG) structured silicon nanowire (SiNW) field-effect transistor (FET) as the transducer and a sodium-selective membrane extended gate (EG) as the sensing unit was developed. The SiNW channel DG FET was fabricated through the dry etching of the silicon-on-insulator substrate by using electrospun polyvinylpyrrolidone nanofibers as a template for the SiNW pattern transfer. The selectivity and sensitivity of sodium to other ions were verified by constructing a sodium ion sensor, wherein the EG was electrically connected to the SiNW channel DG FET with a sodium-selective membrane. An extremely high sensitivity of 1464.66 mV/dec was obtained for a NaCl solution. The low sensitivities of the SiNW channel FET-based sodium ion sensor to CaCl2, KCl, and pH buffer solutions demonstrated its excellent selectivity. The reliability and stability of the sodium ion sensor were verified under non-ideal behaviors by analyzing the hysteresis and drift. Therefore, the SiNW channel DG FET-based sodium ion sensor, which comprises a sodium-selective membrane EG, can be applied to accurately detect sodium ions in the analyses of sweat or blood.

scholarly journals Aptamer–field-effect transistors overcome Debye length limitations for small-molecule sensing

Science ◽  
2018 ◽  
Vol 362 (6412) ◽  
pp. 319-324 ◽  
Author(s):  
Nako Nakatsuka ◽  
Kyung-Ae Yang ◽  
John M. Abendroth ◽  
Kevin M. Cheung ◽  
Xiaobin Xu ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Debye Length ◽  
Stem Loop ◽  
Highly Sensitive ◽  
Close Proximity ◽  
Sphingosine 1 Phosphate ◽  
Specific Receptors ◽  
Highly Sensitive Detection

Detection of analytes by means of field-effect transistors bearing ligand-specific receptors is fundamentally limited by the shielding created by the electrical double layer (the “Debye length” limitation). We detected small molecules under physiological high–ionic strength conditions by modifying printed ultrathin metal-oxide field-effect transistor arrays with deoxyribonucleotide aptamers selected to bind their targets adaptively. Target-induced conformational changes of negatively charged aptamer phosphodiester backbones in close proximity to semiconductor channels gated conductance in physiological buffers, resulting in highly sensitive detection. Sensing of charged and electroneutral targets (serotonin, dopamine, glucose, and sphingosine-1-phosphate) was enabled by specifically isolated aptameric stem-loop receptors.

scholarly journals Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor

Biosensors ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 210
Author(s):  
Kristina A. Malsagova ◽  
Tatyana O. Pleshakova ◽  
Rafael A. Galiullin ◽  
Andrey F. Kozlov ◽  
Ivan D. Shumov ◽  
...  
Keyword(s):  
Protein Detection ◽  
Covalent Immobilization ◽  
Silicon On Insulator ◽  
Target Protein ◽  
Ca 125 ◽  
Minimum Concentration ◽  
Immobilized Antibodies ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Biospecific Interaction

The detection of CA 125 protein in a solution using a silicon-on-insulator (SOI)-nanowire biosensor with n-type chip has been experimentally demonstrated. The surface of nanowires was modified by covalent immobilization of antibodies against CA 125 in order to provide the biospecificity of the target protein detection. We have demonstrated that the biosensor signal, which results from the biospecific interaction between CA 125 and the covalently immobilized antibodies, increases with the increase in the protein concentration. At that, the minimum concentration, at which the target protein was detectable with the SOI-nanowire biosensor, amounted to 1.5 × 10−16 M.

scholarly journals Methods for rapid frequency-domain characterization of leakage currents in silicon nanowire-based field-effect transistors

2014 ◽  
Vol 5 ◽  
pp. 964-972 ◽  
Author(s):  
Tomi Roinila ◽  
Xiao Yu ◽  
Jarmo Verho ◽  
Tie Li ◽  
Pasi Kallio ◽  
...  
Keyword(s):  
Frequency Domain ◽  
Leakage Current ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Low Cost ◽  
Silicon Nanowire ◽  
Binary Sequence ◽  
Practical Applications ◽  
Frequency Domain Methods ◽  
Wide Range

Silicon nanowire-based field-effect transistors (SiNW FETs) have demonstrated the ability of ultrasensitive detection of a wide range of biological and chemical targets. The detection is based on the variation of the conductance of a nanowire channel, which is caused by the target substance. This is seen in the voltage–current behavior between the drain and source. Some current, known as leakage current, flows between the gate and drain, and affects the current between the drain and source. Studies have shown that leakage current is frequency dependent. Measurements of such frequency characteristics can provide valuable tools in validating the functionality of the used transistor. The measurements can also be an advantage in developing new detection technologies utilizing SiNW FETs. The frequency-domain responses can be measured by using a commercial sine-sweep-based network analyzer. However, because the analyzer takes a long time, it effectively prevents the development of most practical applications. Another problem with the method is that in order to produce sinusoids the signal generator has to cope with a large number of signal levels. This may become challenging in developing low-cost applications. This paper presents fast, cost-effective frequency-domain methods with which to obtain the responses within seconds. The inverse-repeat binary sequence (IRS) is applied and the admittance spectroscopy between the drain and source is computed through Fourier methods. The methods is verified by experimental measurements from an n-type SiNW FET.

scholarly journals SOI-Nanowire Biosensor for the Detection of Glioma-Associated miRNAs in Plasma

Chemosensors ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 95
Author(s):  
Kristina A. Malsagova ◽  
Tatyana O. Pleshakova ◽  
Rafael A. Galiullin ◽  
Andrey F. Kozlov ◽  
Tatyana S. Romanova ◽  
...  
Keyword(s):  
Elevated Level ◽  
Silicon Nanowire ◽  
Silicon On Insulator ◽  
Sensor Chip ◽  
Concentration Limit ◽  
Synthetic Analogue ◽  
Oligonucleotide Probes ◽  
System A ◽  
Highly Sensitive ◽  
Oligonucleotide Detection

Herein, we report the development of a highly sensitive nanotechnology-based system—silicon-on-insulator nanowire biosensor for the revelation of microRNAs (miRNAs), associated with the development of glioma in the human. In this system, a sensor chip, bearing an array of silicon nanowire structures, is employed. The sensor chip is fabricated using a top-down technology. In our experiments reported herein, we demonstrated the detection of DNA oligonucleotide (oDNA), which represents a synthetic analogue of microRNA-363 associated with the development of glioma. To provide biospecific detection of the target oligonucleotides, the surface of the nanowire structures is modified with oligonucleotide probes; the latter are complementary to the target ones. The concentration limit of the target oligonucleotide detection, attained using our nanowire biosensor, is at the level of DL~10−17 M. The revelation of the elevated level of glioma-associated miRNA in plasma is also demonstrated.

Site-specific modification of proteins by ExoT-mediated ADP-ribosylation

TECHNOLOGY ◽  
2015 ◽  
Vol 03 (01) ◽  
pp. 72-78
Author(s):  
Chia-Lun Jack Tsai ◽  
Slim Sassi ◽  
Brian Seed
Keyword(s):  
Chemical Diversity ◽  
Chain Reaction ◽  
Site Specific ◽  
Bar Codes ◽  
C Terminus ◽  
Highly Sensitive ◽  
Wide Range ◽  
Polymerase Chain ◽  
Highly Sensitive Detection ◽  
Adp Ribosyltransferase

Proteins bearing short sequence tags (ExoTags) can be specifically modified in a reaction catalyzed by the ADP-ribosyltransferase of Pseudomonas ExoT. ExoT tolerates a wide range of substituents at the adenosine N6 position, allowing the convenient installation of chemical diversity at the site of the tag. The ExoTag can be placed at either the N - or the C -terminus of the protein of interest or internally. The minimum tag is Arg-Leu-Ser-Arg (RLSR). Tandem copies of the minimal ExoTag enhance the efficiency of modification and allow modification at multiple sites within the tag. ExoT can be used to conjugate NAD-RNA to ExoTag bearing proteins, providing a convenient method for introducing nucleic acid bar codes for highly sensitive detection of proteins by polymerase chain reaction.

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