scholarly journals Waveguide-Based Fluorescent Immunosensor for the Simultaneous Detection of Carbofuran and 3-Hydroxy-Carbofuran

Biosensors ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 191
Author(s):  
Weiming Sun ◽  
Lanhua Liu ◽  
Abdul Ghaffar Memon ◽  
Xiaohong Zhou ◽  
Hongwei Zhao
Keyword(s):  
Water Quality ◽  
Monoclonal Antibody ◽  
Human Health ◽  
Broad Spectrum ◽  
Rapid Detection ◽  
Detection Method ◽  
Simultaneous Detection ◽  
Agricultural Products ◽  
Linear Detection ◽  
High Binding Affinity

Carbofuran (CBF) is an efficient and broad-spectrum insecticide. As testing indicators for water quality and agricultural products, CBF and its metabolite 3-hydroxy-carbofuran (3-OH-CBF) are regulated by many countries. The detection of CBF and 3-OH-CBF is of great importance for the environment and human health. However, an immunosensor detection method for the simultaneous analysis of CBF and 3-OH-CBF remains unavailable. Herein, we report a waveguide-based fluorescent immunosensor for detecting CBF and 3-OH-CBF, synchronously. The immunosensor is based on a broad-spectrum monoclonal antibody with high binding affinity against CBF and 3-OH-CBF. The linear detection ranges for CBF and 3-OH-CBF are 0.29–2.69 and 0.12–4.59 μg/L, with limits of detection of 0.13 μg/L for CBF and 0.06 μg/L for 3-OH-CBF, respectively. The whole detection process for each cycle is approximately 30 min. The results show a good application prospect for the rapid detection of CBF and 3-OH-CBF in water or agricultural products.

Simultaneous detection of 4-chlorophenol and 4-nitrophenol using Ti3C2Tx MXene based electrochemical sensor

The Analyst ◽  
2021 ◽  
Author(s):  
Ling Lei ◽  
Caoling Li ◽  
Wensheng Huang ◽  
K. Wu
Keyword(s):  
Electrochemical Sensor ◽  
Rapid Detection ◽  
Detection Method ◽  
Simultaneous Detection ◽  
High Toxicity

Developing sensitive and rapid detection method for 4-chlorophenol (4-CP) and 4-nitrophenol (4-NP) is very important due to their high toxicity. In this work, bulk Ti3AlC2 powder was firstly etched to...

scholarly journals Development of a fluorescent immunochromatographic assay based on quantum dots for the detection of fleroxacin

RSC Advances ◽  
2021 ◽  
Vol 11 (36) ◽  
pp. 22005-22013
Author(s):  
Qingbao Yang ◽  
Yanhua Qi ◽  
Jingming Zhou ◽  
Yumei Chen ◽  
Chao Liang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantum Dots ◽  
Fluorescent Probe ◽  
Rapid Detection ◽  
Detection Method ◽  
Water Soluble ◽  
Fluorescent Detection ◽  
Immunochromatographic Assay

(1) Water-soluble CdSe/ZnS QDs and an anti-FLE monoclonal antibody were used to prepare a fluorescent probe. (2) Primary rapid detection of FLE residues with visual fluorescent detection method.

Development of Enzyme linked Immunosorbent Assay and Lateral Flow Immunoassay for the Rapid Detection of Dapsone in Foods

2021 ◽  
Author(s):  
Chuanlai Xu ◽  
xin guo ◽  
lu lin ◽  
Shanshan Song ◽  
aihong wu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Health ◽  
Immunosorbent Assay ◽  
Food Chain ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Food Animals

The abuse of dapsone (DDS) in food animals could result in its accumulation in humans through the food chain thus endangering human health. A sensitive monoclonal antibody (mAb) against DDS...

Water Quality and Health

1991 ◽  
Vol 23 (1-3) ◽  
pp. 201-209 ◽  
Author(s):  
W. Kreisel
Keyword(s):  
Water Quality ◽  
Developing Countries ◽  
Drinking Water ◽  
Water Supply ◽  
Human Health ◽  
Pollution Control ◽  
Wastewater Reuse ◽  
Water Supplies ◽  
Minimum Quality Standards ◽  
Quality Guidelines

Water quality can affect human health in various ways: through breeding of vectors, presence of pathogenic protozoa, helminths, bacteria and viruses, or through inorganic and organic chemicals. While traditional concern has been with pathogens and gastro-intestinal diseases, chemical pollutants in drinking-water supplies have in many instances reached proportions which affect human health, especially in cases of chronic exposure. Treatment of drinking-water, often grossly inadequate in developing countries, is the last barrier of health protection, but control at source is more effective for pollution control. Several WHO programmes of the International Drinking-Water Supply and Sanitation Decade have stimulated awareness of the importance of water quality in public water supplies. Three main streams have been followed during the eighties: guidelines for drinking-water quality, guidelines for wastewater reuse and the monitoring of freshwater quality. Following massive investments in the community water supply sector to provide people with adequate quantities of drinking-water, it becomes more and more important to also guarantee minimum quality standards. This has been recognized by many water and health authorities in developing countries and, as a result, WHO cooperates with many of them in establishing water quality laboratories and pollution control programmes.

Development of a monoclonal antibody-based immunochromatographic strip for the rapid detection of tigecycline in human serum

2021 ◽  
Author(s):  
Chuanlai Xu ◽  
qianqian lu ◽  
Xin-Xin Xu ◽  
Shanshan Song ◽  
aihong wu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Serum ◽  
Rapid Detection ◽  
Immunochromatographic Assay ◽  
Immunochromatographic Strip

In this study, a gold labelled immunochromatographic assay was developed to detect tigecycline (TGC) in human serum. For this purpose, an anti-TGC monoclonal antibody, 2G7, was produced and characterized, and...

scholarly journals The Detection of Bovine Estrus by Lactoferrin Monoclonal Antibody

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1582
Author(s):  
Jihwan Lee ◽  
Suhyun Lee ◽  
Younbae Park ◽  
Seokhyun Lee ◽  
Seungmin Ha ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Binding Affinity ◽  
Reproductive Performance ◽  
Economic Loss ◽  
Cervical Mucus ◽  
Female Rats ◽  
Economic Damage ◽  
Bovine Lactoferrin ◽  
Estrous Synchronization ◽  
High Binding Affinity

To improve reproductive performance in cattle, the accurate detection of estrus and optimization of insemination relative to ovulation are necessary. However, poor heat detection by farm staff leads to a decreased conception rate, thus inflicting economic damage to the beef and dairy industries. This study aimed to develop monoclonal antibodies (mAb) that can specifically bind to the bovine lactoferrin (bLF) protein, which we have previously demonstrated to be overexpressed in bovine cervical mucus during estrus. Female rats were intraperitoneally immunized with bLF protein as the antigen. Anti-bLF mAbs were then purified by affinity chromatography, and their binding affinity for the bLF antigen was examined using ELISA. We found a high binding affinity between mAbs and bLF. Finally, we developed a rapid bovine heat detection kit using the anti-bLF mAbs that we generated and tested on cervical mucus from 12 cows (estrous synchronization, n = 2; natural cycling, n = 10). We found that the kits accurately detected estrus. Overall, our fabricated heat detection kit based on rat anti-bLF mAbs could pave the way for the development of potent tools for heat detection devices for dairy cattle, thereby preventing economic loss.

scholarly journals MnO2@Reduced Graphene Oxide Nanocomposite-Based Electrochemical Sensor for the Simultaneous Determination of Trace Cd(II), Zn(II) and Cu(II) in Water Samples

Membranes ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 517
Author(s):  
Siyamthanda Hope Mnyipika ◽  
Tshimangadzo Saddam Munonde ◽  
Philiswa Nosizo Nomngongo
Keyword(s):  
Heavy Metals ◽  
Electrochemical Sensor ◽  
Simultaneous Determination ◽  
Rapid Detection ◽  
Electrochemical Sensors ◽  
Simultaneous Detection ◽  
Cyclic Voltammograms ◽  
Current Response ◽  
Mno2 Nanoparticles

The rapid detection of trace metals is one of the most important aspect in achieving environmental monitoring and protection. Electrochemical sensors remain a key solution for rapid detection of heavy metals in environmental water matrices. This paper reports the fabrication of an electrochemical sensor obtained by the simultaneous electrodeposition of MnO2 nanoparticles and RGO nanosheets on the surface of a glassy carbon electrode. The successful electrodeposition was confirmed by the enhanced current response on the cyclic voltammograms. The XRD, HR-SEM/EDX, TEM, FTIR, and BET characterization confirmed the successful synthesis of MnO2 nanoparticles, RGO nanosheets, and MnO2@RGO nanocomposite. The electrochemical studies results revealed that MnO2@RGO@GCE nanocomposite considerably improved the current response on the detection of Zn(II), Cd(II) and Cu(II) ions in surface water. These remarkable improvements were due to the interaction between MnO2 nanomaterials and RGO nanosheets. Moreover, the modified sensor electrode portrayed high sensitivity, reproducibility, and stability on the simultaneous determination of Zn(II), Cd(II), and Cu(II) ions. The detection limits of (S/N = 3) ranged from 0.002–0.015 μg L−1 for the simultaneous detection of Zn(II), Cd(II), and Cu(II) ions. The results show that MnO2@RGO nanocomposite can be successfully used for the early detection of heavy metals with higher sensitivity in water sample analysis.

scholarly journals ACE2-targeting monoclonal antibody as potent and broad-spectrum coronavirus blocker

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Yuning Chen ◽  
Ya-Nan Zhang ◽  
Renhong Yan ◽  
Guifeng Wang ◽  
Yuanyuan Zhang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Broad Spectrum ◽  
Management Strategy ◽  
Spectrum Management ◽  
Clinical Development ◽  
Severe Toxicity ◽  
Converting Enzyme ◽  
Angiotensin Converting Enzyme 2 ◽  
Binding Residues

AbstractThe evolution of coronaviruses, such as SARS-CoV-2, makes broad-spectrum coronavirus preventional or therapeutical strategies highly sought after. Here we report a human angiotensin-converting enzyme 2 (ACE2)-targeting monoclonal antibody, 3E8, blocked the S1-subunits and pseudo-typed virus constructs from multiple coronaviruses including SARS-CoV-2, SARS-CoV-2 mutant variants (SARS-CoV-2-D614G, B.1.1.7, B.1.351, B.1.617.1, and P.1), SARS-CoV and HCoV-NL63, without markedly affecting the physiological activities of ACE2 or causing severe toxicity in ACE2 “knock-in” mice. 3E8 also blocked live SARS-CoV-2 infection in vitro and in a prophylactic mouse model of COVID-19. Cryo-EM and “alanine walk” studies revealed the key binding residues on ACE2 interacting with the CDR3 domain of 3E8 heavy chain. Although full evaluation of safety in non-human primates is necessary before clinical development of 3E8, we provided a potentially potent and “broad-spectrum” management strategy against all coronaviruses that utilize ACE2 as entry receptors and disclosed an anti-coronavirus epitope on human ACE2.

A simple rapid detection method of DNA based on ligation-mediated real-time fluorescence PCR

The Analyst ◽  
2013 ◽  
Vol 138 (19) ◽  
pp. 5745 ◽  
Author(s):  
Yu Qing Du ◽  
Peng Fei Gao ◽  
Wei Wang ◽  
Ting Ting Wang ◽  
Yong Chang ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Detection Method
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