scholarly journals Biocompatibility of Blank, Post-Processed and Coated 3D Printed Resin Structures with Electrogenic Cells

Biosensors ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 152
Author(s):  
Cacie Hart ◽  
Charles M. Didier ◽  
Frank Sommerhage ◽  
Swaminathan Rajaraman
Keyword(s):  
Cell Culture ◽  
3D Printing ◽  
Culture Vessel ◽  
Standard Cell ◽  
Post Processing ◽  
3D Printed ◽  
Processing Techniques

The widespread adaptation of 3D printing in the microfluidic, bioelectronic, and Bio-MEMS communities has been stifled by the lack of investigation into the biocompatibility of commercially available printer resins. By introducing an in-depth post-printing treatment of these resins, their biocompatibility can be dramatically improved up to that of a standard cell culture vessel (99.99%). Additionally, encapsulating resins that are less biocompatible with materials that are common constituents in biosensors further enhances the biocompatibility of the material. This investigation provides a clear pathway toward developing fully functional and biocompatible 3D printed biosensor devices, especially for interfacing with electrogenic cells, utilizing benchtop-based microfabrication, and post-processing techniques.

Custom Design & Fabrication of 3D printed cast for ankle immobilisation

2017 ◽  
Vol 2 (2) ◽  
pp. 98 ◽  
Author(s):  
Guruprasad Kuppu Rao ◽  
Tanmay Shah ◽  
Vijay Dayanand Shetty ◽  
B. Ravi
Keyword(s):  
3D Printing ◽  
Data Acquisition ◽  
Bone Fracture ◽  
Patient Specific ◽  
Post Processing ◽  
Tibia Bone ◽  
Joint Injuries ◽  
Custom Design ◽  
Inner Surface ◽  
3D Printed

<p>Management of bone and joint injuries is commonly done by immobilisation using plaster/fibreglass casts. This study describes design and fabrication of patient specific cast using 3D printing.  The 3D printed cast while being patient friendly is superior to earlier casts in healing efficacy and hence redefines the joint immobilisation practice. We present here a case of “walk on brace” design and fabrication using 3D printing. The custom design of ankle immobilisation cast was done for an 18-year-old boy having tibia bone fracture during gymnastic activity. The workflow comprises of anatomical data acquisition, CAD, 3D printing, post processing and clinical approval for use. Additional features such as straps, anti-slip inner surface and tread for floor grip were incorporated in the design. </p>

Investigation on the Effects of Acetone Vapor-Polishing to Fracture Behavior of ABS Printed Materials at Different Operating Temperature

2020 ◽  
Vol 1005 ◽  
pp. 141-149
Author(s):  
Brian Jumaquio Tuazon ◽  
Michaela Tayag Espino ◽  
John Ryan Cortez Dizon
Keyword(s):  
Rough Surface ◽  
Surface Finish ◽  
Fracture Behavior ◽  
Operating Temperature ◽  
Acetone Vapor ◽  
Post Processing ◽  
3D Printed ◽  
Printed Materials ◽  
Processing Techniques ◽  
Rough Surface Finish

Fused Deposition Modelling (FDM) technology is one of most common technique used in 3D printing as of today for several reasons such as it is low cost and high speed printing capacity. However, common characteristic of FDM 3D printed materials are poor layer adhesion strength and rough surface finish which requires post-processing to improve it. Heat treatment and vapor-polishing are post-processing techniques used to address the poor layer adhesion and rough surface finish of 3D printed materials, respectively. This study will combine these two post-processing techniques and investigate its effect on the mechanical properties of 3D printed materials. The present study describes the effect of acetone vapor-polishing to facture behavior of ABS 3D printed material at higher operating temperatures. The study will compare the fracture behavior of ABS 3D-printed material when polished using acetone vapor bath and tested at high operating temperature to unpolished material. Five replications for each test condition were conducted. All experiment was carried out using ASTM Izod Type E tests with a 2.75J pendulum. The results showed that acetone vapor polishing strongly affects the fracture behavior of ABS 3D printed materials when operating at high temperature.

scholarly journals 3D printing – a key technology for tailored biomedical cell culture lab ware

2016 ◽  
Vol 2 (1) ◽  
pp. 105-108 ◽  
Author(s):  
Florian Schmieder ◽  
Joachim Ströbel ◽  
Mechthild Rösler ◽  
Stefan Grünzner ◽  
Bernd Hohenstein ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Printing ◽  
Ex Vivo ◽  
Vascular Perfusion ◽  
Laboratory Equipment ◽  
Key Technology ◽  
Cell Culture Systems ◽  
First Results ◽  
3D Printed

AbstractToday’s 3D printing technologies offer great possibilities for biomedical researchers to create their own specific laboratory equipment. With respect to the generation of ex vivo vascular perfusion systems this will enable new types of products that will embed complex 3D structures possibly coupled with cell loaded scaffolds closely reflecting the in-vivo environment. Moreover this could lead to microfluidic devices that should be available in small numbers of pieces at moderate prices. Here, we will present first results of such 3D printed cell culture systems made from plastics and show their use for scaffold based applications.

scholarly journals Post-Processing of 3D-Printed Polymers

Technologies ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 61
Author(s):  
John Ryan C. Dizon ◽  
Ciara Catherine L. Gache ◽  
Honelly Mae S. Cascolan ◽  
Lina T. Cancino ◽  
Rigoberto C. Advincula
Keyword(s):  
Additive Manufacturing ◽  
3D Printing ◽  
3D Models ◽  
Industrial Applications ◽  
Post Processing ◽  
Processing Methods ◽  
Stl File ◽  
Advantages And Disadvantages ◽  
Manufacturing Operations ◽  
3D Printed

Additive manufacturing, commonly known as 3D printing, is an advancement over traditional formative manufacturing methods. It can increase efficiency in manufacturing operations highlighting advantages such as rapid prototyping, reduction of waste, reduction of manufacturing time and cost, and increased flexibility in a production setting. The additive manufacturing (AM) process consists of five steps: (1) preparation of 3D models for printing (designing the part/object), (2) conversion to STL file, (3) slicing and setting of 3D printing parameters, (4) actual printing, and (5) finishing/post-processing methods. Very often, the 3D printed part is sufficient by itself without further post-printing processing. However, many applications still require some forms of post-processing, especially those for industrial applications. This review focuses on the importance of different finishing/post-processing methods for 3D-printed polymers. Different 3D printing technologies and materials are considered in presenting the authors’ perspective. The advantages and disadvantages of using these methods are also discussed together with the cost and time in doing the post-processing activities. Lastly, this review also includes discussions on the enhancement of properties such as electrical, mechanical, and chemical, and other characteristics such as geometrical precision, durability, surface properties, and aesthetic value with post-printing processing. Future perspectives is also provided towards the end of this review.

scholarly journals Real-Time Live-Cell Imaging Technology Enables High-Throughput Screening to Verify in Vitro Biocompatibility of 3D Printed Materials

Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2125 ◽  
Author(s):  
Ina G. Siller ◽  
Anton Enders ◽  
Tobias Steinwedel ◽  
Niklas-Maximilian Epping ◽  
Marline Kirsch ◽  
...  
Keyword(s):  
3D Printing ◽  
High Throughput Screening ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Post Processing ◽  
In Vitro Biocompatibility ◽  
3D Printed ◽  
Printed Materials

With growing advances in three-dimensional (3D) printing technology, the availability and diversity of printing materials has rapidly increased over the last years. 3D printing has quickly become a useful tool for biomedical and various laboratory applications, offering a tremendous potential for efficiently fabricating complex devices in a short period of time. However, there still remains a lack of information regarding the impact of printing materials and post-processing techniques on cell behavior. This study introduces real-time live-cell imaging technology as a fast, user-friendly, and high-throughput screening strategy to verify the in vitro biocompatibility of 3D printed materials. Polyacrylate-based photopolymer material was printed using high-resolution 3D printing techniques, post-processed using three different procedures, and then analyzed with respect to its effects on cell viability, apoptosis, and necrosis of adipogenic mesenchymal stem cells (MSCs). When using ethanol for the post-processing procedure and disinfection, no significant effects on MSCs could be detected. For the analyses a novel image-based live-cell analysis system was compared against a biochemical-based standard plate reader assay and traditional flow cytometry. This comparison illustrates the superiority of using image-based detection of in vitro biocompatibility with respect to analysis time, usability, and scientific outcome.

scholarly journals 3D-Printed Sensors and Actuators in Cell Culture and Tissue Engineering: Framework and Research Challenges

Sensors ◽  
2020 ◽  
Vol 20 (19) ◽  
pp. 5617
Author(s):  
Pablo Pérez ◽  
Juan Alfonso Serrano ◽  
Alberto Olmo
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
3D Printing ◽  
Regenerative Medicine ◽  
Electrical Impedance Spectroscopy ◽  
Special Focus ◽  
Tissue Formation ◽  
Sensors And Actuators ◽  
Printed Sensors ◽  
3D Printed

Three-dimensional printing technologies have been recently proposed to monitor cell cultures and implement cell bioreactors for different biological applications. In tissue engineering, the control of tissue formation is crucial to form tissue constructs of clinical relevance, and 3D printing technologies can also play an important role for this purpose. In this work, we study 3D-printed sensors that have been recently used in cell culture and tissue engineering applications in biological laboratories, with a special focus on the technique of electrical impedance spectroscopy. Furthermore, we study new 3D-printed actuators used for the stimulation of stem cells cultures, which is of high importance in the process of tissue formation and regenerative medicine. Key challenges and open issues, such as the use of 3D printing techniques in implantable devices for regenerative medicine, are also discussed.

scholarly journals On the intrinsic sterility of 3D printing

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2661 ◽  
Author(s):  
Russell Y. Neches ◽  
Kaitlin J. Flynn ◽  
Luis Zaman ◽  
Emily Tung ◽  
Nicholas Pudlo
Keyword(s):  
Cell Culture ◽  
3D Printing ◽  
Fabrication Process ◽  
3D Printers ◽  
3D Printed

3D printers that build objects using extruded thermoplastic are quickly becoming commonplace tools in laboratories. We demonstrate that with appropriate handling, these devices are capable of producing sterile components from a non-sterile feedstock of thermoplastic without any treatment after fabrication. The fabrication process itself results in sterilization of the material. The resulting 3D printed components are suitable for a wide variety of applications, including experiments with bacteria and cell culture.

scholarly journals On the intrinsic sterility of 3D printing

2014 ◽  
Author(s):  
Russell Y Neches ◽  
Kaitlin J. Flynn ◽  
Luis Zaman ◽  
Emily Tung ◽  
Nicholas Pudlo
Keyword(s):  
Cell Culture ◽  
3D Printing ◽  
Fabrication Process ◽  
3D Printers ◽  
3D Printed

3D printers that build objects using extruded thermoplastic are quickly becoming common place tools in laboratories. We demonstrate that with appropriate handling, these devices are capable of producing sterile components from a non-sterile feedstock of thermoplastic without any treatment after fabrication. The fabrication process itself results in sterilization of the material. The resulting 3D printed components are suitable for a wide variety of applications, including experiments with bacteria and cell culture.

scholarly journals On the intrinsic sterility of 3D printing

2016 ◽  
Author(s):  
Russell Y Neches ◽  
Kaitlin J. Flynn ◽  
Luis Zaman ◽  
Emily Tung ◽  
Nicholas Pudlo
Keyword(s):  
Cell Culture ◽  
3D Printing ◽  
Fabrication Process ◽  
3D Printers ◽  
3D Printed

3D printers that build objects using extruded thermoplastic are quickly becoming common place tools in laboratories. We demonstrate that with appropriate handling, these devices are capable of producing sterile components from a non-sterile feedstock of thermoplastic without any treatment after fabrication. The fabrication process itself results in sterilization of the material. The resulting 3D printed components are suitable for a wide variety of applications, including experiments with bacteria and cell culture.

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